- Title
- Consecutive bouts of diverse contractile activity alter acute responses in human skeletal muscle
- Creator
- Coffey, V. G.; Pilegaard, H.; Garnham, A. P.; O'Brien, Brendan; Hawley, J. A.
- Date
- 2009
- Type
- Text; Journal article
- Identifier
- http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/45029
- Identifier
- vital:1915
- Identifier
-
https://doi.org/10.1152/japplphysiol.91221.2008
- Identifier
- ISSN:8750-7587
- Abstract
- We examined acute molecular responses in skeletal muscle to divergent exercise stimuli by combining consecutive bouts of resistance and endurance exercise. Eight men [22.9 ± 6.3 yr, body mass of 73.2 ± 4.5 kg, peak O2 uptake (V̇O2peak) of 54.0 ± 5.7 ml·kg-1·min-1] were randomly assigned to complete trials consisting of either resistance exercise (8 x 5 leg extension, 80% 1 repetition maximum) followed by a bout of endurance exercise (30 min cycling, 70% V̇O2peak) or vice versa. Muscle biopsies were obtained from the vastus lateralis at rest, 15 min after each exercise bout, and after 3 h of passive recovery to determine early signaling and mRNA responses. Phosphorylation of Akt and Akt1Ser473 were elevated 15 min after resistance exercise compared with cycling, with the greatest increase observed when resistance exercise followed cycling (∼55%; P < 0.01). TSC2-mTOR-S6 kinase phosphorylation 15 min after each bout of exercise was similar regardless of the exercise mode. The cumulative effect of combined exercise resulted in disparate mRNA responses. IGF-I mRNA content was reduced when cycling preceded resistance exercise (-42%), whereas muscle ring finger mRNA was elevated when cycling was undertaken after resistance exercise (∼52%; P < 0.05). The hexokinase II mRNA level was higher after resistance cycling (∼45%; P < 0.05) than after cycling-resistance exercise, whereas modest increases in peroxisome proliferator-activated receptor gamma coactivator-1α mRNA did not reveal an order effect. We conclude that acute responses to diverse bouts of contractile activity are modified by the exercise order. Moreover, undertaking divergent exercise in close proximity influences the acute molecular profile and likely exacerbates acute "interference." Copyright © 2009 the American Physiological Society.
- Relation
- Journal of Applied Physiology Vol. 106, no. 4 (2009), p. 1187-1197
- Rights
- Copyright American Physiological Society
- Rights
- Open Access
- Rights
- This metadata is freely available under a CCO license
- Subject
- Acute responses; Concurrent training; Endurance
- Full Text
- Reviewed
- Hits: 1175
- Visitors: 1553
- Downloads: 435
Thumbnail | File | Description | Size | Format | |||
---|---|---|---|---|---|---|---|
View Details Download | SOURCE1 | Published Version | 514 KB | Adobe Acrobat PDF | View Details Download |