Evaluation of the role of galectins in parasite immunity
- Authors: Preston, Sarah , Dunphy, Jillian , Beddoe, Travis , Meeusen, Els , Young, Anna
- Date: 2014
- Type: Text , Book chapter
- Relation: Galectins: Methods and Protocols (Methods in Molecular Biology series) Chapter 25 p. 371-395
- Full Text: false
- Reviewed:
- Description: Galectin-11 and galectin-14 are ruminant galectins involved in parasitic infections. Although their roles in parasite immunity are still being elucidated, its appears that their functions are parasite specific. In gastrointestinal infections with the nematode Haemonchus contortus, both galectin-11 and galectin-14 appear to be protective. However, in a chronic infection of liver fluke, Fasciola hepatica, these galectins may aid parasite survival. This chapter discusses the methods designed to study parasitic infections in sheep, which have provided us with insight into the functions of galectin-11 and galectin-14 during host–parasite interactions. These methods include parasite cultivation and infection, galectin staining of host and parasite tissue, surface staining of parasites with recombinant galectins and in vitro assays to monitor the effect of galectins on larval development. © Springer Science+Business Media New York 2015.
Evaluation of the role of galectins in parasite immunity
- Authors: Swan, Jaclyn , Sakthivel, Dhanasekaran , Beddoe, Travis , Stear, Michael , Piedrafita, David , Preston, Sarah
- Date: 2022
- Type: Text , Book chapter
- Relation: Galectins: Methods in Molecular Biology Chapter 7 p. 475-515
- Full Text: false
- Reviewed:
- Description: Galectin-11 (LGALS-11) and galectin-14 (LGALS-14) are ruminant specific galectins, first reported in sheep. Although their roles in parasite immunity are still being elucidated, it appears that they influence protection against parasites. In gastrointestinal infections with the nematode Haemonchus contortus, both galectin-11 and galectin-14 appear to be protective. However, in a chronic infection of liver fluke, Fasciola hepatica, these galectins may aid parasite survival. To unravel the structural, functional, and ligand profile of galectin-11 and galectin-14, recombinant production of these proteins is vital. Here we present the recombinant production of soluble galectin-11 and galectin-14 from domestic sheep for in vitro and structural biology studies. These methods include parasite cultivation and infection, galectin staining of host and parasite tissue, surface staining of parasites with recombinant galectins, pull-down assays to identify endogenous galectin binding proteins, and in vitro assays to monitor the effect of galectins on parasite development. © 2022, Springer Science+Business Media, LLC, part of Springer Nature.