- Title
- Novel method using 3-dimensional segmentation in spectral domain-optical coherence tomography imaging in the chick reveals defocus-induced regional and time-sensitive asymmetries in the choroidal thickness
- Creator
- Nava, Diane; Antony, Bhavna; Zhang, L. I.; Abràmoff, Michael; Wildsoet, Christine
- Date
- 2016
- Type
- Text; Journal article
- Identifier
- http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/197513
- Identifier
- vital:18889
- Identifier
-
https://doi.org/10.1017/S0952523816000067
- Identifier
- ISSN:0952-5238
- Abstract
- Studies into the mechanisms underlying the active emmetropization process by which neonatal refractive errors are corrected, have described rapid, compensatory changes in the thickness of the choroidal layer in response to imposed optical defocus. While high frequency A-scan ultrasonography, as traditionally used to characterize such changes, offers good resolution of central (on-axis) changes, evidence of local retinal control mechanisms make it imperative that more peripheral, off-axis changes also be tracked. In this study, we used in vivo high resolution spectral domain-optical coherence tomography (SD-OCT) imaging in combination with the Iowa Reference Algorithms for 3-dimensional segmentation, to more fully characterize these changes, both spatially and temporally, in young, 7-day old chicks (n = 15), which were fitted with monocular +15 D defocusing lenses to induce choroidal thickening. With these tools, we were also able to localize the retinal area centralis, which was used as a landmark along with the ocular pectin in standardizing the location of scans and aligning them for subsequent analyses of choroidal thickness (CT) changes across time and between eyes. Values were derived for each of four quadrants, centered on the area centralis, and global CT values were also derived for all eyes. Data were compared with on-axis changes measured using ultrasonography. There were significant on-axis choroidal thickening that was detected after just one day of lens wear (
- Publisher
- Cambridge University Press
- Relation
- Vis Neurosci Vol. 33, no. (2016), p. E010-E010
- Rights
- All metadata describing materials held in, or linked to, the repository is freely available under a CC0 licence
- Rights
- Copyright Cambridge
- Subject
- Algorithms; Animals; Axial Length, Eye - pathology; Biomedical engineering; Chickens; Choroid - diagnostic imaging; Choroid - pathology; clinical medicine; Coherence (signal processing); Disease Models, Animal; Emmetropia - physiology; Eye - growth & development; eye diseases; Imaging, Three-Dimensional; In vivo Retinal Imaging; Lens (optics); Materials science; Medical and health sciences; Monocular; neurology & neurosurgery; ophthalmology & optometry; Optical coherence tomography; Organ Size; Refractive Errors - physiopathology; Resolution (electron density); Retinal; Segmentation; Sense organs; Time Factors; Tomography; Tomography, Optical Coherence
- Reviewed
- Hits: 463
- Visitors: 461
- Downloads: 0