Evaluation of the performance of Ortho T. cruzi ELISA test system for the detection of antibodies to Trypanosoma cruzi
- Rivera, Hilda, McAuliffe, Isabel, Aderohunmu, TaLesa, Wiegand, Ryan, Montgomery, Susan, Bradbury, Richard, Handali, Sukwan
- Authors: Rivera, Hilda , McAuliffe, Isabel , Aderohunmu, TaLesa , Wiegand, Ryan , Montgomery, Susan , Bradbury, Richard , Handali, Sukwan
- Date: 2022
- Type: Text , Journal article
- Relation: Journal of Clinical Microbiology Vol. 60, no. 8 (2022), p.
- Full Text:
- Reviewed:
- Description: The serologic diagnosis of chronic Chagas disease, caused by infection with the parasite Trypanosoma cruzi, is challenging and lacks a gold-standard assay. To overcome the problem, CDC uses an algorithm that uses two tests on different platforms and applies a third test as a tiebreaker. The Ortho T. cruzi ELISA Test System from Ortho Diagnostics was cleared by FDA for clinical diagnosis usage. We evaluated this test against the CDC algorithm for chronic Chagas disease. We tested several sets of serum specimens: 104 specimens tested positive for T. cruzi specific antibody and 283 (including 30 specimens positive for antibody to Leishmania spp.) tested negative based on the current CDC chronic T. cruzi infection diagnostic testing algorithm. Concordance of the Ortho T. cruzi ELISA Test System with the CDC algorithm result was 90% (95% CI 87 to 93%) overall and 92% (95% CI 89 to 95%) when excluding Leishmania spp. antibody positive specimens. The cross-reactivity of the Ortho T. cruzi ELISA Test System was 37% to Leishmania spp. serologically positive specimens, 1% to specimens from patients diagnosed with other parasitic infections, and 0% against specimens from a US noninfected population. In conclusion, the Ortho T. cruzi ELISA Test System compares well against the CDC diagnostic algorithm for chronic Chagas disease. The availability of this FDA-cleared assay will improve the chronic Chagas disease diagnosis. © 2022 American Society for Microbiology. All rights reserved.
- Authors: Rivera, Hilda , McAuliffe, Isabel , Aderohunmu, TaLesa , Wiegand, Ryan , Montgomery, Susan , Bradbury, Richard , Handali, Sukwan
- Date: 2022
- Type: Text , Journal article
- Relation: Journal of Clinical Microbiology Vol. 60, no. 8 (2022), p.
- Full Text:
- Reviewed:
- Description: The serologic diagnosis of chronic Chagas disease, caused by infection with the parasite Trypanosoma cruzi, is challenging and lacks a gold-standard assay. To overcome the problem, CDC uses an algorithm that uses two tests on different platforms and applies a third test as a tiebreaker. The Ortho T. cruzi ELISA Test System from Ortho Diagnostics was cleared by FDA for clinical diagnosis usage. We evaluated this test against the CDC algorithm for chronic Chagas disease. We tested several sets of serum specimens: 104 specimens tested positive for T. cruzi specific antibody and 283 (including 30 specimens positive for antibody to Leishmania spp.) tested negative based on the current CDC chronic T. cruzi infection diagnostic testing algorithm. Concordance of the Ortho T. cruzi ELISA Test System with the CDC algorithm result was 90% (95% CI 87 to 93%) overall and 92% (95% CI 89 to 95%) when excluding Leishmania spp. antibody positive specimens. The cross-reactivity of the Ortho T. cruzi ELISA Test System was 37% to Leishmania spp. serologically positive specimens, 1% to specimens from patients diagnosed with other parasitic infections, and 0% against specimens from a US noninfected population. In conclusion, the Ortho T. cruzi ELISA Test System compares well against the CDC diagnostic algorithm for chronic Chagas disease. The availability of this FDA-cleared assay will improve the chronic Chagas disease diagnosis. © 2022 American Society for Microbiology. All rights reserved.
Medical parasitology taxonomy update, January 2018 to May 2020
- Mathison, Blaine, Bradbury, Richard, Pritt, Bobbi
- Authors: Mathison, Blaine , Bradbury, Richard , Pritt, Bobbi
- Date: 2021
- Type: Text , Journal article , Review
- Relation: Journal of Clinical Microbiology Vol. 59, no. 2 (2021), p.
- Full Text:
- Reviewed:
- Description: The taxonomy of parasites of medical and public health importance is rapidly evolving. This minireview provides an update of taxonomic revisions and additions in the field of medical parasitology from January 2018 to May 2020. Several established human parasites have been reassigned to different genera over the past 2 years, while a number of novel parasites of humans have been identified. A comprehensive summary of these changes is provided here, and Taenia suihominis is proposed as a replacement name for Taenia asiaticus Eom et al., which is a homonym of Taenia asiatica von Linstow. © 2021 American Society for Microbiology. All rights reserved. Erratum: Medical parasitology taxonomy update, January 2018 to May 2020 (Journal of Clinical Microbiology (2021) 59:2 (e01308-20) DOI: https://doi.org/10.1128/JCM.01308-20
- Authors: Mathison, Blaine , Bradbury, Richard , Pritt, Bobbi
- Date: 2021
- Type: Text , Journal article , Review
- Relation: Journal of Clinical Microbiology Vol. 59, no. 2 (2021), p.
- Full Text:
- Reviewed:
- Description: The taxonomy of parasites of medical and public health importance is rapidly evolving. This minireview provides an update of taxonomic revisions and additions in the field of medical parasitology from January 2018 to May 2020. Several established human parasites have been reassigned to different genera over the past 2 years, while a number of novel parasites of humans have been identified. A comprehensive summary of these changes is provided here, and Taenia suihominis is proposed as a replacement name for Taenia asiaticus Eom et al., which is a homonym of Taenia asiatica von Linstow. © 2021 American Society for Microbiology. All rights reserved. Erratum: Medical parasitology taxonomy update, January 2018 to May 2020 (Journal of Clinical Microbiology (2021) 59:2 (e01308-20) DOI: https://doi.org/10.1128/JCM.01308-20
Where have all the diagnostic morphological parasitologists gone?
- Bradbury, Richard, Sapp, Sarah, Potters, Idzi, Mathison, Blaine, Frean, John, Mewara, Abhishek, Sheorey, Harsha, Tamarozzi, Francesca, Couturier, Marc, Chiodini, Peter, Pritt, Bobbi
- Authors: Bradbury, Richard , Sapp, Sarah , Potters, Idzi , Mathison, Blaine , Frean, John , Mewara, Abhishek , Sheorey, Harsha , Tamarozzi, Francesca , Couturier, Marc , Chiodini, Peter , Pritt, Bobbi
- Date: 2022
- Type: Text , Journal article
- Relation: Journal of Clinical Microbiology Vol. 60, no. 11 (2022), p.
- Full Text:
- Reviewed:
- Description: Advances in laboratory techniques have revolutionized parasitology diagnostics over the past several decades. Widespread implementation of rapid antigen detection tests has greatly expanded access to tests for global parasitic threats such as malaria, while next-generation amplification and sequencing methods allow for sensitive and specific detection of human and animal parasites in complex specimen matrices. Recently, the introduction of multiplex panels for human gastrointestinal infections has enhanced the identification of common intestinal protozoa in feces along with bacterial and viral pathogens. Despite the benefits provided by novel diagnostics, increased reliance on nonmicroscopy-based methods has contributed to the progressive, widespread loss of morphology expertise for parasite identification. Loss of microscopy and morphology skills has the potential to negatively impact patient care, public health, and epidemiology. Molecular- and antigen-based diagnostics are not available for all parasites and may not be suitable for all specimen types and clinical settings. Furthermore, inadequate morphology experience may lead to missed and inaccurate diagnoses and erroneous descriptions of new human parasitic diseases. This commentary highlights the need to maintain expert microscopy and morphological parasitology diagnostic skills within the medical and scientific community. We proposed that light microscopy remains an important part of training and practice in the diagnosis of parasitic diseases and that efforts should be made to train the next generation of morphological parasitologists before the requisite knowledge, skills, and capacity for this complex and important mode of diagnosis are lost. In summary, the widespread, progressive loss of morphology expertise for parasite identification negatively impacts patient care, public health, and epidemiology. © 2022 American Society for Microbiology.
- Authors: Bradbury, Richard , Sapp, Sarah , Potters, Idzi , Mathison, Blaine , Frean, John , Mewara, Abhishek , Sheorey, Harsha , Tamarozzi, Francesca , Couturier, Marc , Chiodini, Peter , Pritt, Bobbi
- Date: 2022
- Type: Text , Journal article
- Relation: Journal of Clinical Microbiology Vol. 60, no. 11 (2022), p.
- Full Text:
- Reviewed:
- Description: Advances in laboratory techniques have revolutionized parasitology diagnostics over the past several decades. Widespread implementation of rapid antigen detection tests has greatly expanded access to tests for global parasitic threats such as malaria, while next-generation amplification and sequencing methods allow for sensitive and specific detection of human and animal parasites in complex specimen matrices. Recently, the introduction of multiplex panels for human gastrointestinal infections has enhanced the identification of common intestinal protozoa in feces along with bacterial and viral pathogens. Despite the benefits provided by novel diagnostics, increased reliance on nonmicroscopy-based methods has contributed to the progressive, widespread loss of morphology expertise for parasite identification. Loss of microscopy and morphology skills has the potential to negatively impact patient care, public health, and epidemiology. Molecular- and antigen-based diagnostics are not available for all parasites and may not be suitable for all specimen types and clinical settings. Furthermore, inadequate morphology experience may lead to missed and inaccurate diagnoses and erroneous descriptions of new human parasitic diseases. This commentary highlights the need to maintain expert microscopy and morphological parasitology diagnostic skills within the medical and scientific community. We proposed that light microscopy remains an important part of training and practice in the diagnosis of parasitic diseases and that efforts should be made to train the next generation of morphological parasitologists before the requisite knowledge, skills, and capacity for this complex and important mode of diagnosis are lost. In summary, the widespread, progressive loss of morphology expertise for parasite identification negatively impacts patient care, public health, and epidemiology. © 2022 American Society for Microbiology.
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