A field trial to test effects of watering, seed addition and disturbance on perennial species recruitment in Belah woodland
- Authors: Callister, Katrina , Westbrooke, Martin
- Date: 2017
- Type: Text , Journal article
- Relation: Ecological Management and Restoration Vol. 18, no. 3 (2017), p. 246-249
- Full Text: false
- Reviewed:
- Description: Failure of perennial species to regenerate is a significant threat to semi-arid woodlands across south-eastern Australia. High grazing pressure eliminates the recruitment of many perennial species in semi-arid woodlands, but little is known about requirements for regeneration under low grazing pressure. We tested the effects of addition of water (irrigation to match the largest rainfall events of the last century), seed, soil disturbance and fire within a grazing exclosure in Belah (Casuarina pauper) woodland in the Murray-Sunset National Park, Victoria. Recruitment was observed in 13 perennial species and was dominated by chenopods. Addition of water, seed and soil disturbance increased abundance of juvenile perennial species above the low-level background recruitment that occurred in the prevailing drought conditions. This supports the view that continuous recruitment occurs for many semi-arid perennials. Low seed availability and an inability to maintain soil moisture conditions matching that of regeneration events are likely factors in the lack of recruitment for tree species and limited response of shrubs in this experiment. © 2017 Ecological Society of Australia and John Wiley & Sons Australia, Ltd
A linear optimization problem for repetitive processes
- Authors: Dymkou, Siarhei
- Date: 2002
- Type: Text , Journal article
- Relation: The National Academy of Sciences of Belarus Vol. 4, no. (2002), p. 35-44
- Full Text: false
- Reviewed:
- Description: C1
- Description: 2003000106
A Markov-blanket-based model for gene regulatory network inference
- Authors: Ram, Ramesh , Chetty, Madhu
- Date: 2011
- Type: Text , Journal article
- Relation: Transactions on Computational Biology and Bioinformatics Vol. 8, no. 2 (2011), p.
- Full Text: false
- Reviewed:
- Description: An efficient two-step Markov blanket method for modeling and inferring complex regulatory networks from large-scale microarray data sets is presented. The inferred gene regulatory network (GRN) is based on the time series gene expression data capturing the underlying gene interactions. For constructing a highly accurate GRN, the proposed method performs: 1) discovery of a gene's Markov Blanket (MB), 2) formulation of a flexible measure to determine the network's quality, 3) efficient searching with the aid of a guided genetic algorithm, and 4) pruning to obtain a minimal set of correct interactions. Investigations are carried out using both synthetic as well as yeast cell cycle gene expression data sets. The realistic synthetic data sets validate the robustness of the method by varying topology, sample size, time delay, noise, vertex in-degree, and the presence of hidden nodes. It is shown that the proposed approach has excellent inferential capabilities and high accuracy even in the presence of noise. The gene network inferred from yeast cell cycle data is investigated for its biological relevance using well-known interactions, sequence analysis, motif patterns, and GO data. Further, novel interactions are predicted for the unknown genes of the network and their influence on other genes is also discussed.
A roadmap to generate renewable protein binders to the human proteome
- Authors: Colwill, Karen , Persson, Helena , Jarvik, Nicholas , Wyrzucki, Arkadiusz , Wojcik, John , Koide, Akiko , Kossiakoff, Anthony , Koide, Shohei , Sidhu, Sachdev , Dyson, Michael , Pershad, Kritika , Pavlovic, John , Karatt-Vellatt, Aneesh , Schofield, Darren , Kay, Brian , McCafferty, John , Mersmann, Michael , Meier, Doris , Mersmann, Jana , Helmsing, Saskia , Hust, Michael , Dubel, Stefan , Berkowicz, Susan , Freemantle, Alexia , Spiegel, Michael , Sawyer, Alan , Layton, Daniel , Nice, Edouard , Dai, Anna , Rocks, Oliver , Williton, Kelly , Fellouse, Frederic , Hersi, Kadija , Pawson, Tony , Nilsson, Peter , Sundberg, Marten , Sjoberg, Ronald , Sivertsson, Asa , Schwenk, Jochen , Takanen, Jenny , Hober, Sophia , Uhlen, Mathias , Dahlgren, Lars-Goran , Flores, Alex , Johansson, Ida , Weigelt, Johan , Crombet, Lissette , Loppnau, Peter , Kozieradzki, Ivona , Cossar, Doug , Arrowsmith, C. , Edwards, Aled , Graslund, Susanne
- Date: 2011
- Type: Text , Journal article
- Relation: Nature Methods Vol. 8, no. 7 (2011), p. 551-558
- Full Text: false
- Reviewed:
- Description: Despite the wealth of commercially available antibodies to human proteins, research is often hindered by their inconsistent validation, their poor performance and the inadequate coverage of the proteome. These issues could be addressed by systematic, genome-wide efforts to generate and validate renewable protein binders. We report a multicenter study to assess the potential of hybridoma and phage-display technologies in a coordinated large-scale antibody generation and validation effort. We produced over 1,000 antibodies targeting 20 SH2 domain proteins and evaluated them for potency and specificity by enzyme-linked immunosorbent assay (ELISA), protein microarray and surface plasmon resonance (SPR). We also tested selected antibodies in immunoprecipitation, immunoblotting and immunofluorescence assays. Our results show that high-affinity, high-specificity renewable antibodies generated by different technologies can be produced quickly and efficiently. We believe that this work serves as a foundation and template for future larger-scale studies to create renewable protein binders.
A sequential extraction procedure for acid sulfate soils : Partitioning of iron
- Authors: Claff, Salirian , Sullivan, Leigh , Burton, Edward , Bush, Richard
- Date: 2010
- Type: Text , Journal article
- Relation: Geoderma Vol. 155, no. 3-4 (2010), p. 224-230
- Full Text: false
- Reviewed:
- Description: A new sequential extraction scheme for acid sulfate soil materials has been evaluated for iron partitioning in a range of synthetic iron-bearing minerals and natural acid sulfate soil materials. This sequential extraction procedure employs six steps to quantify (1) exchangeable (magnesium chloride extractable), (2) acid (hydrochloric acid) soluble, (3) reactive organic-bound (pyrophosphate extractable), (4) crystalline oxide (citrate buffered dithionite (CBD)) extractable, (5) pyrite-bound (nitric acid extractable) and (6) residual (acid/peroxide digestible) forms of iron. Given its intended use for acid sulfate soil materials that frequently contain pyrite, a primary aim of this new sequential extraction procedure was to differentiate iron bound in pyrite from iron contained in other minerals. The results demonstrated that dissolution of pyrite was effectively isolated in the pyrite-bound extraction step, with dissolution of other iron mineral phases (i.e. akaganeite, ferrihydrite, goethite, hematite, jarosite, magnetite, and schwertmannite) occurring within the other five extraction steps. Following a systematic examination of these synthetic iron mineral phases, the sequential extraction scheme was applied to an acid sulfate soil profile, with detailed data presented for two soil layers: one representative of the sulfidic (unoxidised) conditions, and the other sulfuric (oxidised) conditions. Partitioning data for pyrite-bound iron in the acid sulfate soil profile showed good agreement with that calculated via the independently measured pyrite-bound reduced sulfur. This study indicates that the new sequential extraction procedure is suitable for the assessment of iron partitioning in acid sulfate soil materials. © 2009 Elsevier B.V. All rights reserved.
A small-scale test for rapid assessment of the soil development potential in post-mining soils
- Authors: Bucka, Franziska , Pihlap, Evelin , Kaiser, Jara , Baumgartl, Thomas , Kögel-Knabner, Ingrid
- Date: 2021
- Type: Text , Journal article
- Relation: Soil and Tillage Research Vol. 211, no. (2021), p.
- Full Text: false
- Reviewed:
- Description: When ceasing brown coal mining activities, the restoration of the disturbed landscape is required, and a rapid development of functional soils is of utter importance for the rehabilitation of these areas. In order to accelerate soil development and rehabilitation, soils are designed or engineered by applying alternative approaches in order to construct a soil-like substrate, e.g., by mixing different substrates and organic carbon (OC) sources. We used a rapid and easy to perform laboratory approach to simulate the initial steps of soil development. We investigated the mixtures’ properties and the initial development of soil structure, in order to identify suitable soil mixtures for subsequent field trials. We tested six different mixtures composed of increasing complexity, based on the mixture used for a rehabilitation program at a coal mine in southern Australia. The components were overburden, fly ash, paper mulch, brown coal and plant litter. We performed a short-term laboratory incubation in regularly irrigated microcosms for forty days at constant water tension. Our results showed that the addition of fly ash to the overburden led to a higher moisture content. Fly ash together with paper mulch and brown coal improved nutrient supply and OC content, but led also to a very wide C/N ratio >95. The molecular composition of the paper mulch and brown coal OC shows the potential for long-term OC storage because of slow microbial degradation. Microbial activity, as measured by CO2 release, was high in all mixtures with litter addition, but only the additional presence of fly ash, brown coal and paper mulch led to a higher microbial carbon use efficiency (CUE). Soil structure formation, as measured by isolating water-stable aggregates, was induced in all mixtures and intensified in the presence of litter. In the mixtures with litter, there was a predominant formation of large macroaggregates (0.63−30 mm) which stored >80 % of the total OC. We suggest that the complex rehabilitation mixture indicates the potential for soil structure development within a short timeframe also in field scale, because the tested substrates are known to store moisture, deliver nutrients and OC for sustainable microbial growth. © 2021 Elsevier B.V.
Abundant stocks and mobilization of elements in boreal acid sulfate soils
- Authors: Yli-Halla, Markku , Virtanen, Seija , Mäkelä, Minna , Simojoki, Asko , Hirvi, Mirva , Innanen, Saila , Mäkelä, Jaakko , Sullivan, Leigh
- Date: 2017
- Type: Text , Journal article
- Relation: Geoderma Vol. 308, no. (2017), p. 333-340
- Full Text: false
- Reviewed:
- Description: Large amounts of sulfate and divalent iron (Fe) are released into the pore water of acid sulfate (AS) soils upon oxidation of sulfidic materials. The simultaneously produced acidity dissolves metals from the soil matrix. Reduced horizons of AS soils commonly have a large mineral nitrogen (N) stock in the form of NH4 +-N, which is a potential source of N leaching and gaseous emissions. This study was carried out at three AS soil sites in Finland. Cation composition of pore water was monitored in a monolithic lysimeter experiment. Timothy samples grown in an AS soil were analysed for mineral elements and the soil was investigated for zinc (Zn) distribution in different chemical species at four depths down to 85 cm. The composition of pipestems formed in previous root channels was investigated by SEM, X-ray EDX and XRD. Emissions of CO2 and N2O were measured in an AS field which had a peaty topsoil. Monitoring by closed chambers was carried out at three sites differing in the depth of peat layer (15, 30 and 60 cm). In the sulfuric horizon, large amounts of calcium and magnesium were mobilized. Reflooding stopped the mobilization of those elements but resulted in abundant dissolution of Fe, which became the dominant cation in the pore water. Timothy growing in an AS soil showed only small deviations from the average composition measured in Finland with the exception of Zn that was at the deficiency level. As Zn in the root zone had been dissolved and subsequently leached, it was recovered in easily soluble forms in the subsoil. Pipestem composition indicated accumulation of Fe and formation of a new solid phase probably mostly in the form of schwertmannite even though jarosite was also detected. The annual CO2 emissions from the peaty AS soil were about 6000 kg C ha− 1 but the N2O emissions were relatively small, with a magnitude more typical of mineral rather than organic soils. Thus, large N stock of an AS soil do not necessarily contribute to abundant gaseous N emissions. © 2017 Elsevier B.V.
Activation of proteases in an anaerobic sulphidogenic bioreactor
- Authors: Whiteley, Chris , Pletschke, Brett , Rose, Peter , Tshivhunge, Sylvia , Watson, Shaun , Whittington-Jones, Kevin
- Date: 2004
- Type: Text , Journal article
- Relation: Biotechnology Letters Vol. 26, no. 1 (2004), p. 55-59
- Full Text: false
- Reviewed:
- Description: Activities of proteases were stimulated by specific sulphur metabolites during the enhanced hydrolysis of complex polymeric organic carbon in an anaerobic sulphidogenic environment. While sulphate at 1000 mg l(-1) inhibited proteases by 50%, there was a 2.5-fold increase in activity of proteases by added sulphite and a 3.6-fold increase from added sulphide. Two hypothetical models are proposed. First the sulphur species, sulphite (HSO3-) and sulphide (HS-), liberated at different times during the sulphate reduction process, directly activate the proteases, which are associated with the organic particulate matter, leading to a subsequent enhancement of hydrolysis of polymeric material. Second, they indirectly activate the proteases by neutralising the cations on the floc surface disrupting the integrity of the organic particulate floc therebye releasing further entrapped enzymes from the organic particulate matter.
Age-related differences revealed in australian fur seal arctocephalus pusillus doriferus gut microbiota
- Authors: Smith, Stuart , Chalker, Andrea , Dewar, Meagan , Arnould, John
- Date: 2013
- Type: Text , Journal article
- Relation: FEMS Microbiology Ecology Vol. 86, no. 2 (2013), p. 246-255
- Full Text: false
- Reviewed:
- Description: The gut microbiota of Australian fur seals (Arctocephalus pusillus doriferus) was examined at different age classes using fluorescent in situ hybridisation (FISH) and 16S rRNA gene pyrosequencing. The FISH results indicated that in the fur seal groups, the predominant phyla are Firmicutes (22.14-67.33%) followed by Bacteroidetes (3.11-15.45%) and then Actinobacteria (1.4-5.9%) consistent with other mammals. Phylum Proteobacteria had an initial abundance of 1.8% in the 2-month-old pups, but < 1% of bacterial numbers for the other fur seal age groups. Significant differences did occur in the abundance of Clostridia, Lactobacilli and Bifidobacteria between 2 months pups and 9 months pups and adult fur seals. Results from the 16S rRNA gene pyrosequencing supported the FISH data and identified significant differences in the composition of Firmicutes, Bacteroidetes, Actinobacteria, Proteobacteria, Verrucomicrobia and Fusobacteria at all ages. Class Clostridia in phylum Firmicutes dominates the microbiota of the 2 months and 9 months seal pups, whilst class Bacilli dominates the 6 months pups. In addition, a high level of dissimilarity was observed between all age classes. This study provides novel insight into the gut microbiota of Australian fur seals at different age classes. © 2013 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.
An antarctic biogeographical anomaly resolved: the true identity of a widespread species of Collembola
- Authors: Greenslade, Penelope
- Date: 2018
- Type: Text , Journal article
- Relation: Polar Biology Vol. 41, no. 5 (2018), p. 969-981
- Full Text: false
- Reviewed:
- Description: Springtails and mites are the most abundant and species rich micro arthropods in the Antarctic and species tend to be short-range endemics. It was thought that the Springtail (Collembola), Friesea grisea (Schäffer) was an exception. It was described briefly nearly 150 years ago from South Georgia and has not been redescribed from the type locality since. However, published localities include the South Shetland Islands, the Antarctic Peninsula, Enderby Land, Marguerite Bay and South and North Victoria Land suggesting that it is unusually widespread. Recent sequence data have indicated that some Antarctic populations could be putative species but individuals were allegedly morphologically identical. No sequence data were available for South Georgian specimens. These results suggested that a new examination of the South Georgian species’ morphology was needed in order to resolve the anomalous biogeographic patterns. I here redescribe F. grisea from South Georgia and show that it has subtle morphological characters that distinguish it from Antarctic individuals allowing it to be unambiguously distinguished from all known Antarctic populations. Consequently, Friesea antarctica is taken out of synonymy with F. grisea. These results emphasise the endemism of Antarctic faunas and allows more targeted conservation planning, especially for short-range endemic species located in likely refugia during glacial periods. Descriptions of putative species from the Continental populations are currently in progress. A new Friesea species from South Georgia, F. fantaba, is also described here. The genus Friesea is now the most species-rich genus of terrestrial arthropods in both the Antarctic and subantarctic and includes twelve species.
An assessment of riparian restoration outcomes in two rural catchments in south-western Victoria : Focusing on tree and shrub species richness, structure and recruitment characteristics
- Authors: Wevill, Tricia , Florentine, Singarayer
- Date: 2014
- Type: Text , Journal article
- Relation: Ecological Management and Restoration Vol. 15, no. 2 (2014), p. 133-139
- Full Text: false
- Reviewed:
- Description: Riparian ecosystems are among the most degraded systems in the landscape, and there has been substantial investment in their restoration. Consequently, monitoring restoration interventions offers opportunities to further develop the science of riparian restoration, particularly how to move from small-scale implementation to a broader landscape scale. Here, we report on a broad range of riparian revegetation projects in two regions of south-western Victoria, the Corangamite and Glenelg-Hopkins Catchment Management Areas. The objectives of restoration interventions in these regions have been stated quite broadly, for example, to reinstate terrestrial habitat and biodiversity, control erosion and improve water quality. This study reports on tree and shrub composition, structure and recruitment after restoration works compared with remnant vegetation found regionally. Within each catchment, a total of 57 sites from six subcatchments were identified, representing three age-classes: <4, 4-8 and >8-12 years after treatment, as well as untreated (control) sites. Treatments comprised fencing to exclude stock, spraying or slashing to reduce weed cover, followed by planting with tube stock. Across the six subcatchments, 12 reference (remnant) sites were used to provide a benchmark for species richness, structural and recruitment characteristics and to aid interpretation of the effects of the restoration intervention. Vegetation structure was well developed in the treated sites by 4-8 years after treatment. However, structural complexity was higher at remnant sites than at treated or untreated sites due to a higher richness of small shrubs. Tree and shrub recruitment occurred in all remnant sites and at 64% of sites treated >4 years ago. Most seedling recruitment at treatment sites was by Acacia spp. This assessment provides data on species richness, structure and recruitment characteristics following restoration interventions. Data from this study will contribute to longitudinal studies of vegetation processes in riparian landscapes of south-western Victoria. © 2014 Ecological Society of Australia and Wiley Publishing Asia Pty Ltd.
Ancylostoma ceylanicum
- Authors: Colella, Vito , Bradbury, Richard , Traub, Rebecca
- Date: 2021
- Type: Text , Journal article
- Relation: Trends in Parasitology Vol. 37, no. 9 (2021), p. 844-845
- Full Text: false
- Reviewed:
Assessing capture and tagging methods for brolgas, Antigone rubicunda (Gruidae)
- Authors: Veltheim, Inka , Chavez-Ramirez, Felipe , Hill, Richard , Cook, Simon
- Date: 2015
- Type: Text , Journal article
- Relation: Wildlife Research Vol. 42, no. 5 (2015), p. 373-381
- Full Text: false
- Reviewed:
- Description: Context Studies assessing welfare issues and efficiency of animal capture methods are important, as capture can pose a significant welfare risk and methods can be time consuming to develop. It is imperative to choose methods that minimise injuries, maximise capture rates and minimise time spent on capture. Aims To test methods of crane capture and tagging (transmitter, colour and metal band fitting), and to compare and develop safe and time efficient methods for capturing brolgas (Antigone rubicunda). Methods We tested three types of noose traps, cannon nets, clap-nets, spotlighting at night roosts and active pursuit of pre-fledged chicks. We also tested two band sizes and two transmitter attachment methods. We compared the success and capture efficiency of these methods and considered welfare issues by comparing the number of injuries and mortalities resulting from these methods. Key results We successfully captured brolgas with noose traps and a cannon net, and by using active pursuit of pre-fledged chicks. Noose traps became more efficient when deployed with call playback and taxidermy decoys. Australian noose traps and active pursuit of pre-fledged chicks were the safest methods with fewest injuries. Conclusions For maximum capture success with minimum injuries to target and non-target species, we recommend noose traps with call playback and taxidermy decoys for capturing adult and juvenile brolgas at feeding areas, and active pursuit for pre-fledged chicks at breeding sites. Noose lines should have elastic at both pegged ends, to avoid injuries. To minimise injuries from tagging, we recommend leg-band-fitted transmitters in preference to harness-fitted transmitters, Australian size 35 bands and colour bands with an internal diameter of 22mm. Implications The use of Australian noose traps with call playback and taxidermy decoys is a safe and time efficient method for capturing brolgas, and is also likely to work for other crane species. Our recommendations can help reduce capture-related mortalities and injuries to brolgas (and potentially other cranes), which is crucial, given the brolga is a threatened species. © 2015 CSIRO.
Bird responses to riparian management of degraded lowland streams in southeastern Australia
- Authors: Hale, Robin , Reich, Paul , Johnson, Matthew , Hansen, Birgita , Lake, Philip , Thomson, James , Mac Nally, Ralph
- Date: 2015
- Type: Text , Journal article
- Relation: Restoration Ecology Vol. 23, no. 2 (2015), p. 104-112
- Full Text: false
- Reviewed:
- Description: We assessed the degree to which fencing, livestock exclusion, and replanting of riparian zones affected avian assemblages in massively cleared landscapes. Measurements were made at three creeks in the southern Murray-Darling Basin in southeastern Australia, each of which had circa 1-km long treated sections and paired "untreated" circa 1-km sections, where no fencing, planting, or stock exclusion was done. We measured the change in vegetation characteristics and abundances of native birds for up to 8 years after works were completed. Prior to data collection, we developed expected responses of bird species based on the anticipated time-courses of change in vegetation structure. We used hierarchical Bayesian models to explore the effects of the management actions, and to account for within-site variation in vegetation characteristics. There were major changes in vegetation structure (reductions in bare ground and increases in shrubs and tree recruitment) but avian responses generally were small and not as expected. There are at least four possible reasons for the limited avian responses: (1) there has been a long-term decline in woodland birds across the region; (2) the study was conducted during the longest drought in the instrumental record in the study region; (3) the total amount of replanted vegetation was small in a massively denuded region; and (4) monitoring may have been over too short a term to detect responses to longer-term changes in structural vegetation. © 2014 Society for Ecological Restoration.
Bloodstream infection due to Acinetobacter spp: Epidemiology, risk factors and impact of multi-drug resistance
- Authors: Wareham, David , Bean, David , Khanna, Pooja , Hennessy, Enid , Krahe, Daniel , Ely, Andrew , Millar, Michael
- Date: 2008
- Type: Text , Journal article
- Relation: European Journal of Clinical Microbiology and Infectious Diseases Vol. 27, no. 7 (2008), p. 607-612
- Full Text: false
- Reviewed:
- Description: Acinetobacter spp. are increasingly reported as important causes of human infection. Many isolates exhibit multi-drug resistance, raising concerns over our ability to treat serious infections with these organisms. The impact of infection on clinical outcome as well as the importance of multi-drug resistance is poorly defined. A descriptive retrospective observational study was undertaken of all episodes of Acinetobacter bacteremia occurring in a UK tertiary care centre from 1998-2006. Demographics of infected patients, characteristics and antimicrobial susceptibility of infecting strains were recorded and the impact of antimicrobial therapy on all causes of 30-day mortality assessed. Three hundred ninety-nine episodes of Acinetobacter bacteremia were identified, with A. baumannii being the most frequently isolated species. Most episodes occurred in critical care and were associated with multidrug resistance, with carbapenem resistance rising from 0% in 1998 to 55% in 2006. Although bacteremia due to carbapenem-resistant Acinetobacter and a requirement for critical care were associated with a higher mortality, mortality was not reduced by the administration of appropriate empirical antimicrobial therapy. A prospective study is required to identify both the most effective intervention and those most likely to benefit from treatment.
- Description: C1
Broad-scale patterns in plant diversity vary between land uses in a variegated temperate Australian agricultural landscape
- Authors: Schultz, Nick , Reid, Nick , Lodge, Greg , Hunter, John
- Date: 2014
- Type: Text , Journal article
- Relation: Austral Ecology Vol. 39, no. 7 (2014), p. 855-863
- Full Text: false
- Reviewed:
- Description: Plant diversity is threatened in many agricultural landscapes. Our understanding of patterns of plant diversity in these landscapes is mainly based on small-scale (<1000 m2) observations of species richness. However, such observations are insufficient for detecting the spatial heterogeneity of vegetation composition. In a case-study farm on the North-West Slopes of New South Wales, Australia, we observed species richness at four scales (quadrat, patch, land use and landscape) across five land uses (grazed and ungrazed woodlands, native pastures, roadsides and crops). We applied two landscape ecological models to assess the contribution of these land uses to landscape species richness: (i) additive partitioning of diversity at multiple spatial scales, and (ii) a measure of habitat specificity – the effective number of species that a patch contributes to landscape species richness. Native pastures had less variation between patches than grazed and ungrazed woodlands, and hence were less species-rich at the landscape scale, despite having similar richness to woodlands at the quadrat and patch scale. Habitat specificity was significantly higher for ungrazed woodland patches than all other land uses. Our results showed that in this landscape, ungrazed woodland patches had a higher contribution than the grazed land uses to landscape species richness. These results have implications for the conservation management of this landscape, and highlighted the need for greater consensus on the influence of different land uses on landscape patterns of plant diversity.
Carbon bio-sequestration within the phytoliths of economic bamboo species
- Authors: Parr, Jeffrey , Sullivan, Leigh , Chen, Bihua , Ye, Gongfu , Zheng, Weipeng
- Date: 2010
- Type: Text , Journal article
- Relation: Global Change Biology Vol. 16, no. 10 (2010), p. 2661-2667
- Full Text: false
- Reviewed:
- Description: The rates of carbon bio-sequestration within silica phytoliths of the leaf litter of 10 economically important bamboo species indicates that (a) there is considerable variation in the content of carbon occluded within the phytoliths (PhytOC) of the leaves between different bamboo species, (b) this variation does not appear to be directly related to the quantity of silica in the plant but rather the efficiency of carbon encapsulation by the silica. The PhytOC content of the species under the experimental conditions ranged from 1.6% to 4% of the leaf silica weight. The potential phytolith carbon bio-sequestration rates in the leaf-litter component for the bamboos ranged up to 0.7 tonnes of carbon dioxide (CO
Challenges in applying scientific evidence to width recommendations for riparian management in agricultural Australia
- Authors: Hansen, Birgita , Reich, Paul , Cavagnaro, Timothy , Lake, Philip
- Date: 2015
- Type: Text , Journal article
- Relation: Ecological Management and Restoration Vol. 16, no. 1 (2015), p. 50-57
- Full Text: false
- Reviewed:
- Description: Intact riparian zones maintain aquatic-terrestrial ecosystem function and ultimately, waterway health. Effective riparian management is a major step towards improving the condition of waterways and usually involves the creation of a 'buffer' by fencing off the stream and planting vegetation. Determination of buffer widths often reflects logistical constraints (e.g. private land ownership, existing infrastructure) of riparian and adjacent areas, rather than relying on rigorous science. We used published information to support riparian width recommendations for waterways in agricultural Victoria, Australia. We focused on different ecological management objectives (e.g. nutrient reduction or erosion control) and scrutinised the applicability of data across different environmental contexts (e.g. adjacent land use or geomorphology). Not surprisingly, the evidence supported variable 'effective' riparian widths, depending on the objective and environmental context. We used this information to develop a framework for determining riparian buffer widths to meet a variety of ecological objectives in south-east Australia. Widths for reducing nutrient inputs to waterways were most strongly supported with quantitative evidence and varied between 20 and 38 m depending on environmental context. The environmental context was inconsistently reported, making it difficult to recommend appropriate widths, under different land-use and physiographic scenarios. The evidence to guide width determination generally had high levels of uncertainty. Despite the considerable amount of published riparian research, there was insufficient evidence to demonstrate that implemented widths achieved ecological objectives. We emphasise the need for managers to clearly articulate the objectives of proposed riparian management and carefully consider the environmental context. Monitoring ecological responses associated with different riparian buffer widths is essential to support future management decisions.
Changes in dominance of dipteran families on Coral Sea cays over ten years during a period of substantial vegetation change
- Authors: Rich, Deborah , Greenslade, Penelope , Bickel, Dan
- Date: 2014
- Type: Text , Journal article
- Relation: Northern Territory Naturalist Vol. 25, no. (2014), p. 64-76
- Full Text: false
- Reviewed:
- Description: The habitat on the Coringa-Herald group of coral cays within the northern Coral Sea underwent profound change after about 2000 because of extensive dieback of the dominant forest trees. This work summarises surveys on these cays in 1995, 1996, 1997 and 2007, in order to understand the effect on the native insect fauna of the introduced biological control agent, the non-specific predatory ladybird beetle Cryptolaemus montrouzieri (Coleoptera: Coccinellidae), that was liberated to control scale insects (Hemiptera: Coccidae) whose infestation was responsible for the dieback of the forest trees. This paper documents the families of Diptera (flies) collected on the surveys and it records changes in presence and abundance of Chloropidae, Phoridae, Sarcophagidae, Canacidae, Lonchaeidae and Chironomidae sampled in pitfall traps and yellow pans. Despite some differences in collecting methods and seasons between surveys, there is evidence of substantial changes in the presence and relative abundance of these families. The decline of several families on Coringa Cay and on North East Herald Cay between 1995/96 and 2007 could be the result of a trophic cascade of species loss following tree dieback. One of the most conspicuous changes was that Chloropidae increased on North East Herald Cay between 1997 and 2007 following increases in populations of scale insects, but chloropids are not thought to have had a direct role in the control of scale insects.
Changes in the leukocyte methylome and its effect on cardiovascular-related genes after exercise
- Authors: Denham, Joshua , O'Brien, Brendan , Marques, Francine , Charchar, Fadi
- Date: 2015
- Type: Text , Journal article
- Relation: Journal of Applied Physiology Vol. 118, no. 4 (2015), p. 475-488
- Relation: http://purl.org/au-research/grants/nhmrc/1009490
- Full Text: false
- Reviewed:
- Description: Physical exercise has proven cardiovascular benefits, yet there is no clear understanding of the related molecular mechanisms leading to this. Here we determined the beneficial epigenetic effects of exercise after sprint interval training, a form of exercise known to improve cardiometabolic health. We quantified genome-wide leukocyte DNA methylation of 12 healthy young (18-24 yr) men before and after 4 wk (thrice weekly) of sprint interval training using the 450K BeadChip (Illumina) and validated gene expression changes in an extra seven subjects. Exercise increased subjects' cardiorespiratory fitness and maximal running performance, and decreased low-density lipoprotein cholesterol concentration in conjunction with genome-wide DNA methylation changes. Notably, many CpG island and gene promoter regions were demethylated after exercise, indicating increased genome-wide transcriptional changes. Among genes with DNA methylation changes, epidermal growth factor (EGF), a ligand of the epidermal growth factor receptor known to be involved in cardiovascular disease, was demethylated and showed decreased mRNA expression. Additionally, we found that in microRNAs miR-21 and miR-210, gene DNA methylation was altered by exercise causing a cascade effect on the expression of the mature microRNA involved in cardiovascular function. Our findings demonstrate that exercise alters DNA methylation in circulating blood cells in microRNA and protein-coding genes associated with cardiovascular physiology. Copyright © 2015 the American Physiological Society