${session.getAttribute("locale")} 5 Learning a trade in New Zealand : On and off the job learning in engineering trades Wed 11 Jul 2018 16:23:57 AEST ]]> Investigations into the ecology and management of the invasive plant Galenia pubescens within the native temperate grasslands of Victoria, Australia 80% at a depth of only 0.5 cm in soil. This suggests that fire regimes might be useful in removing mature plants and controlling seed numbers on the surface, and that subsequent native seeding of undisturbed areas may assist in the long-term management of this noxious weed. A trial was conducted to determine the effect of treatment with a plant essential oil (pine oil) to limit the seed germination and seedling emergence of G. pubescens. This trial showed that the effects of pine oil application were significant (P<0.05). Germination was completely inhibited by application of pine oil at 5% or higher concentrations directly to seeds, and seedling emergence was reduced by between 90 and 100% in pot trials. These outcomes demonstrate the potential viability of pine oil as a long-term control option for this species. In field-based experiments, control strategies including herbicide control with glyphosate, organic herbicide control with pine oil, the application of mulch, and the addition of native seeds to the available seedbank (and all possible combinations of these techniques), were tested. The study showed that one single treatment of a G. pubescens infestation without undertaking a secondary treatment is insufficient to control the G. pubescens infestation or to encourage native regeneration, but that a combined strategy, employing all the aforementioned techniques, is more effective. It is however suggested that full regeneration of the area may not be possible unless further restoration programs are instituted after the cycle of G. pubescens’ treatment has been completed. The thesis also assessed the control effects of chemical control combined with a prescribed spring burning. Assessment of the resulting aboveground vegetation of G. pubescens has shown that a combination of chemical control and late-spring burning can reduce the cover of non-native species such as G. pubescens, suggesting that this could be a useful tool in their management. Finally, this study has supported the view that the growth of G. pubescens will be significantly enhanced in a future climate with an enriched atmospheric CO2 concentration. These climatic changes will have important implications for management of this noxious weed in the future.]]> Wed 08 Nov 2017 07:26:34 AEDT ]]> Becoming "Brave and Gallant" : Decolonising the myths of Burke and Wills; Cross-cultural exchanges and the co-production of knowledge during the Victorian Exploring Expedition and the subsequent Relief Expeditions Wed 08 Nov 2017 06:56:03 AEDT ]]> A conceptual re-alignment of methodology underpinning tax effect accounting : An Australian exploration of the contemporary normalising effect Wed 08 Nov 2017 06:48:41 AEDT ]]> Cultural influence on China's household saving Wed 06 Jun 2018 11:24:06 AEST ]]> The development of the Malaysian vocational education and training system Wed 05 Dec 2018 16:37:11 AEDT ]]> Applying prospect-refuge theory to a local environment : My house and the nature strip outside my house Wed 04 Jul 2018 16:39:03 AEST ]]> Video game classification in Australia : Does it enable parents to make informed game choices for their children Wed 04 Jul 2018 16:36:58 AEST ]]> Assessing productive soil - landscapes in Victoria using digital soil mapping Wed 04 Jul 2018 16:35:25 AEST ]]> Accurate and efficient clustering algorithms for very large data sets Wed 04 Jul 2018 16:30:48 AEST ]]> Progressive rebels of Boy's Own Adventure? The 1935 Australian Cricket tour of India; breaking down social and racial barriers Wed 04 Jul 2018 16:28:30 AEST ]]> Injury epidemiology among Australian female cricketers Wed 04 Jul 2018 16:18:15 AEST ]]> The immanence of traumatic rupture : From the extra/ordinary to the originary Wed 04 Jul 2018 16:16:47 AEST ]]> Evaluation of knowledge translation and workforce development through targeted exercise for falls prevention education programs for exercise physiologists Wed 04 Jul 2018 16:15:42 AEST ]]> Motion picture production : A micro-budget model Wed 04 Jul 2018 16:13:34 AEST ]]> Enhancing the implementation of injury prevention exercise programs in professional soccer Wed 04 Jul 2018 16:11:27 AEST ]]> About regularity properties in variational analysis and applications in optimization Wed 04 Jul 2018 16:07:30 AEST ]]> Percy : A life in China - The life and times of Percy Nettle : 1886-1964 Wed 04 Jul 2018 16:06:03 AEST ]]> Employee perception on causes and forms of conflict in the Botswana contruction industry : A comparative study between domestic-owned and Chinese-owned companies Wed 04 Jul 2018 16:04:01 AEST ]]> A three tier forensic model for automatic identification of evidence of child exploitation by analysing the content of chat-logs Wed 04 Jul 2018 16:01:16 AEST ]]> International students and social connectedness : The role sport can play Wed 04 Jul 2018 15:58:39 AEST ]]> Enhancing integration of specialised exercise training into coach practice to prevent lower-limb injury : Using theory and exploring coaches' salient beliefs Wed 04 Jul 2018 15:56:20 AEST ]]> Historical mine sites as modern-day sources of contamination : Measurement and characterisation of arsenic in historical gold mine wastes to identify the potential for mobility and human exposure Wed 04 Jul 2018 15:54:49 AEST ]]> The inimitable Mr Meek : Re-discovering a lost art Wed 04 Jul 2018 15:48:53 AEST ]]> Reflective leadership : A self-study of practice Wed 04 Jul 2018 15:46:13 AEST ]]> Pixel N-grams for Mammographic Image Classification Wed 04 Jul 2018 15:40:56 AEST ]]> Negotiating policy - renegotiating practice : Understanding language, literacy and numeracy teachers' professional identities within discursive weather systems Wed 04 Jul 2018 15:39:33 AEST ]]> Microencapsulation of omega-3 fatty acids from flaxseed oil in flaxseed protein and flaxseed gum based matrix Wed 04 Jul 2018 15:36:43 AEST ]]> Firm growth by women-owned Small and Medium Enterprises in a developing economy setting Wed 04 Jul 2018 15:30:52 AEST ]]> Integrating human communication strategies with project management for effective outcomes Wed 04 Jul 2018 15:29:29 AEST ]]> Market valuation of junior natural resources companies Wed 04 Jul 2018 15:26:42 AEST ]]> Theoretical perspectives on using epidemiology and systems thinking to better understand the aetiology and prevention of distance running-related injury Wed 04 Jul 2018 15:25:24 AEST ]]> Enculturated beliefs: A grounded theory inquiry into club rugby coaching in Australia, South Africa and New Zealand Wed 04 Jul 2018 15:20:14 AEST ]]> Optimality conditions for nonsmooth optimization problems via generalised derivatives Wed 04 Jul 2018 15:18:52 AEST ]]> An Australian Rules for radicals? Community activism and genuine empowerment Wed 04 Jul 2018 15:17:25 AEST ]]> Misogynology and the impossibility of dwelling Wed 04 Jul 2018 14:56:03 AEST ]]> Framed by reconciliation : Reading cross-cultural space in early twenty-first century Australian literature Wed 04 Jul 2018 14:54:59 AEST ]]> Reliability-based design optimisation methods in large scale systems Wed 04 Jul 2018 14:43:28 AEST ]]> Co-encapsulation of Omega-3 fatty acids and probiotic bacteria through complex coacervation Wed 04 Jul 2018 14:42:09 AEST ]]> Telomere, DNA Methylation and Gene Expression changes caused by exercise training 0.05). This finding indicates that cardiorespiratory fitness is an important determinant of telomere biology. Together, these two cross-sectional studies suggest that regular endurance exercise training is associated with longer leukocytes telomeres and that this is likely achieved through higher TPP1 and TERT mRNA expression gained through improved cardiorespiratory fitness. The findings in Chapters 3 and 4 provide evidence for extensive endurance exercise training as an effective lifestyle strategy to attenuate biological ageing. In parallel to telomere length changes, epigenetic modifications (e.g. DNA methylation) caused by environmental factors alter the transcriptomic milieu of cells. My thorough literature review (Chapter 5) revealed that exercise training seems to rearrange chromatin by modifying the DNA methylome in a variety of cells and that the extent is dictated by exercise duration and intensity. Therefore, in the second part of my thesis, I investigated the DNA methylation changes in leukocytes (which are somatic cells) and sperm (male germ cells) from healthy men before and after sprint interval training (SIT). Unlike traditional, long duration training at moderate intensity training, SIT involves short, intense (>85% VO2max to supra-maximal) efforts followed by periods of rest (3–4 min), typically repeated 3–8 times. It is an effective type of training that improves cardiorespiratory fitness quicker than traditional long slow distance training. Thus, to establish the DNA methylome changes associated with SIT, I conducted two training studies and analysed the leukocyte and sperm methylomes using the Infinium HumanMethylation450 BeadChip (Illumina). My third study (Chapter 6) provides the first evidence showing an association between DNA methylation changes paralleled with improvements to lipid profile and cardiorespiratory fitness in humans. Twelve young men (18–24 years) undertook SIT (thrice weekly) for four weeks. Resting blood samples were obtained and whole-blood leukocytes were isolated by red blood cell lysis. Genome-wide DNA methylation was assessed using the 450K BeadChip (Illumina). Cardiorespiratory fitness, determined by maximal oxygen uptake, was improved by 2.1 and low-density lipo-protein cholesterol was decreased by 3.9% after SIT (p < 0.05). Notably, the leukocyte methylome was significantly affected by SIT, in regions throughout the genome in relation to CpG islands – CpG islands, North shores, N shelves, South shores and South shelve – and the nearest genes – 3’ untranslated region (UTR), 5’ UTR, exonic, intergenic, intronic, non-coding and promoter regions (all p < 0.001). Genes with differentially methylated CpG sites (q < 0.005) after SIT were enriched for cardiovascular gene ontology (GO) terms that included metabolic activity, biological adhesion and antioxidant activity. Similarly, pathway analysis revealed genes involved in focal adhesion, calcium signaling and mitogen activated protein kinase were modulated by SIT-induced DNA methylation changes. Amongst the 205,987 probes relating 32,445 transcripts differentially methylated after SIT (q < 0.05), with methylation changes between 0.1 – 62.8%, the largest and most statistically significant demethylated site was in the epidermal growth factor (EGF) gene, causing decreased mRNA expression. As with EGF, the microRNA-21 and microRNA-210 genes (MIR21 and MIR210, respectively), known for their roles in cardiovascular disease (ischemic heart disease and coronary atherosclerosis), had modest but consistently statistically significant DNA methylation changes at numerous CpG sites, which altered mature microRNA abundance. Together, these data suggest that genome-wide DNA methylation changes occur after short-term intense exercise training concurrently with improvements to blood cholesterol profile and cardiorespiratory fitness. The data presented in this thesis provided evidence that the epigenome of somatic cells is malleable to exercise. There is mounting evidence supporting the premise that environmental perturbations cause DNA methylation changes and these are subsequently transgenerationally inherited, altering phenotypes of future generations. In the current study I also asked the question; can exercise training reconfigure the DNA methylome of male germ cells (sperm)? Therefore, my next study (Chapter 7) entails an analysis of the impact that three months of SIT has on genome-wide DNA methylation of sperm in healthy men. Thirteen subjects undertook twice-weekly SIT for three months, while the controls were asked not to change their current physical activity habits (if any). Sperm samples were donated before and after the three-month intervention. Mature sperm were isolated using density gradient centrifugation and DNA was extracted using the Purelink Genomic DNA Mini Kit (Life Technologies). Global and genome-wide DNA methylation was assessed using an enzyme-linked immunosorbent assay-based kit and the 450K BeadChip (Illumina), respectively. Relative to controls, the cases decreased their resting heart rate and had a higher maximal treadmill speed during exercise testing (both p < 0.05). Cases had decreased global DNA methylation after SIT compared to controls (p < 0.05). Genome-wide DNA methylation analysis revealed numerous modest (0.3 – 6%) methylation changes to 7509 CpG sites, relating to 4602 transcripts (q ≤ 0.1). Differentially methylated CpG sites were in genes associated with developmental biology, which included GO terms, such as developmental process, anatomical structure, embryonic morphogenesis and organ development, together with known pathways regulated by exercise training (MAPK, ErbB and PI3K-Akt signalling). Genes with increased methylation were associated with numerous human diseases, with most overrepresented being psychiatric disorders (schizophrenia, Parkinson’s disease and autism). Notably, paternally imprinted genes associated with other diseases were also differentially methylated after SIT. Therefore, exercise training is associated with the modifications to genome-wide DNA methylation of both somatic and germ cells. In conclusion, the studies presented as a series of peer-reviewed publications, outlines investigations that describe an influence of strenuous exercise training on leukocyte telomere length regulation and the DNA methylome of both leukocytes and germ cells. Both of these molecular changes in leukocytes and sperm provide evidence for novel molecular mechanisms by which exercise improves cardiovascular health and fitness. Future investigations should focus on longitudinal studies determining whether these changes are required for improved health and fitness, and should establish whether exercise-induced DNA methylation changes are transgenerationally inherited, and if so, what impact this has to future generations. Such discoveries could change national physical activity guidelines and policies, by emphasising the benefit of regular exercise both in the present and to future offspring.]]> Wed 04 Jul 2018 14:33:41 AEST ]]> Dr James Stewart : Irish doctor and philanthropist on the Ballarat goldfields Wed 04 Jul 2018 14:32:10 AEST ]]> Optimising the management of invasive aquatic plants targeted for extirpation from catchments and waterways : Utilising alligator weed (Alternanthera philoxeroides (Mart.) Griseb.) as a target species Wed 04 Jul 2018 14:26:18 AEST ]]> Canonical Duality Theory for Global Optimization problems and applications Wed 04 Jul 2018 14:06:01 AEST ]]> Contemporary applications of Fresco : The narrative of the artist Wed 04 Jul 2018 14:04:43 AEST ]]> The role of Telomeres in the development of Cardiac Hypertrophy Tue 19 Feb 2019 09:26:34 AEDT ]]> Gas chromatographic-mass spectrometry analysis of volatile organic compounds from cancer cell cultures - The effect of hypoxia Tue 18 Dec 2018 16:21:22 AEDT ]]> Of railways, wine and flowers : A biography of George William Knight Tue 18 Dec 2018 15:18:22 AEDT ]]> Rainfall prediction in Australia : Clusterwise linear regression approach Tue 17 Jul 2018 11:32:52 AEST ]]> Avenue and Arch : Ballarat's commemoration. How are community attitudes to war and peace reflected in the civic management of the Avenue of Honour and the Arch of Victory? Tue 16 Jul 2019 13:59:01 AEST ]]> Teacher pedagogies of dialogic imagination - A narrative inquiry Tue 10 Jul 2018 11:36:27 AEST ]]> First World War Avenues of Honour : Social history through the landscape Tue 09 Jul 2019 12:21:28 AEST ]]> Physical activity promotion in physiotherapy practice Tue 09 Jul 2019 12:18:44 AEST ]]> What is the value of meaning created through social learning in informal virtual communities? Tue 08 May 2018 14:35:44 AEST ]]> Sustainable practices in small and medium sized enterprises in regional Australia Tue 08 May 2018 13:25:39 AEST ]]> Capital budgeting practices and firm performance : A comparative study of Australia and Sri Lanka Tue 08 May 2018 13:21:22 AEST ]]> Managing risk and enhancing corporate sustainability in the Australian extractive sector : An exploratory study of leading mining and oil & gas firms in Australia Tue 08 May 2018 12:24:58 AEST ]]> An investigation into the relationships between novel Y chromosome-linked long non-coding RNAs and coronary artery disease Tue 08 May 2018 12:18:57 AEST ]]> Effective and efficient kernel-based image representations for classification and retrieval Tue 06 Nov 2018 15:32:07 AEDT ]]> Investigating the impact of participation in alternative education from a positive youth development perspective : A case study of the School for Student Leadership Tue 06 Nov 2018 14:50:08 AEDT ]]> Relationships between pumping costs and water quality in optimal operation of regional multiquality water distribution systems Tue 05 Mar 2019 10:34:49 AEDT ]]> Mining a rich lode : The making of the Springdallah Deep Lead Goldfield communities Tue 05 Mar 2019 10:25:39 AEDT ]]> Detection and prevention of the dispersal of the seeds of Chilean needle grass (Nassella neesiana Trin. & Rupr. (Barkworth)) in hay bales Tue 05 Mar 2019 10:22:43 AEDT ]]> Create, curate, cooperate : Exploring the process of sport safety resource development Tue 03 Jul 2018 16:42:25 AEST ]]> Higher education massification and pedagogic adaptation : An investigation of business teaching excellence in inclusive university environments Tue 03 Jul 2018 16:38:51 AEST ]]> Classification of network information flow analysis (CONIFA) to detect new application versions Tue 03 Jul 2018 16:32:40 AEST ]]> Cardiovascular data analytics for real time patient monitoring Tue 03 Jul 2018 16:12:29 AEST ]]> Causes, magnitude and implications of Griefing in Massively Multiplayer Online Role-Playing Games Tue 03 Jul 2018 15:58:48 AEST ]]> The influence of controlled fire on the mobilization of Potentially Toxic Elements (PTE) in a mined landscape : Implications for land management and environmental health Tue 03 Jul 2018 15:22:51 AEST ]]> Anti-war, radical youth revolt, Victoria, 1965-1975 Thu 24 Jan 2019 16:00:14 AEDT ]]> Assessing healthcare providers' performance with and without risk adjustment Thu 24 Jan 2019 15:04:50 AEDT ]]> Organisational change in Department of Defence : A comparison of change management acceptance and interdependencies between military and non-military personnel Thu 23 May 2019 12:40:08 AEST ]]> Structural analysis of Online Romance Scams by applying the transtheoretical model in conjunction with the theory of personal love stories Thu 23 Aug 2018 12:19:54 AEST ]]> Innate-like T cells in patients with cancer Thu 20 Dec 2018 13:52:26 AEDT ]]> Visiting Friends and Relatives (VFR) travel in Australia : An examination of the role of VFR hosts Thu 20 Dec 2018 10:38:52 AEDT ]]> Tertiary student connectedness : Intervention influence on student connectedness as measured in health and academic behaviours of regional tertiary students Thu 20 Dec 2018 10:31:51 AEDT ]]> Ecological practice : Performance making in the age of the anthropocene Thu 20 Dec 2018 10:10:51 AEDT ]]> Algae-based models to configure consumptive flows for ecological benefit in the highly regulated MacKenzie River, south-east Australia Thu 17 May 2018 11:57:00 AEST ]]> Bi what means : Paratextual and filmic representations of bisexuality in contemporary cinema Thu 09 May 2019 17:02:53 AEST ]]> Design and modelling of the limaçon positive displacement gas expander Thu 08 Nov 2018 14:54:07 AEDT ]]> Manipulating a conditioning activity to enhance potentiation and its application to jumping and aprinting performance Thu 08 Nov 2018 14:47:07 AEDT ]]> Teachers' emotional intelligence as a predisposition for discrimiation against students with severe emotional and behavioural disorders Thu 08 Nov 2018 14:33:08 AEDT ]]> A financial stress index to model and forecast financial stress in Australia Thu 08 Nov 2018 14:33:07 AEDT ]]> Reducing climate change related fugitive greenhouse gas emissions from operational longwall coal mines Thu 04 Jul 2019 15:52:48 AEST ]]> Investigating bird responses to fire in the Heathy Dry Forests of Victoria, Australia Mon 29 Apr 2019 10:29:16 AEST ]]> Cognitive behaviour therapy for insomnia and depression among older adults : A mixed methods randomised controlled clinical trial Mon 24 Jun 2019 10:06:00 AEST ]]> Epidemiology, injury and illness prevention in Olympic combat sports Mon 17 Dec 2018 14:41:22 AEDT ]]> Dr Fanny Reading : 'A clever little bird' Mon 13 Aug 2018 12:13:52 AEST ]]> Development and evaluation of optimization based data mining techniques analysis of brain data Mon 09 Jul 2018 16:41:22 AEST ]]> James Curtis and spiritualism in Nineteenth-Century Ballarat Mon 09 Jul 2018 16:32:14 AEST ]]> Your stories, my stories, our stories : Power/knowledge relations and Koorie perspectives in discourses of Australian History Education Mon 09 Jul 2018 16:28:57 AEST ]]> Effective leadership behaviours to manage people issues of cross border mergers and aquisitions in China Mon 09 Jul 2018 16:27:25 AEST ]]> Organisational resilience within a complexity science framework : A case study of Ballarat City Council Mon 09 Jul 2018 15:58:02 AEST ]]> The contribution of silverfish (insecta: zygentoma) to Australian invertebrate biodiversity and endemism 4% divergence in COI sequences and each geographically restricted. Intracluster divergences are also large, and despite the well-supported phylogeny no clear “barcode gap” (distinction between intracluster and intercluster distances) was found for three of the six NSW populations. The 28S data distinguished only four of the six COI clusters from NSW with essentially no variation within each cluster. The 28S data generally aligned well with morphological evidence, clearly identifying Heterolepisma sp. B as a distinct species, and supporting also the description of Heterolepisma sp. A even though it only appears to differ from H. sclerophylla in the number of styli. Similar genetic distances are observed in 28S data for H. sclerophylla populations from North Nowra, Glenbrook/Burralow/Nattai and Megalong, however the Broulee and Wellington populations have identical 28S sequences. The low levels of variation in 28S sequences between NSW populations accord with the lack of unambiguous morphological differences.]]> Mon 09 Jul 2018 15:51:59 AEST ]]> Tourism governance for sustainable heritage tourism in Sri Lankan heritage destinations Mon 09 Jul 2018 15:50:04 AEST ]]> A collaborative exploration of creativity, doodling and flow : A practice-based education inquiry Mon 07 Jan 2019 13:20:56 AEDT ]]> A new perceptual dissimilarity measure for image retrieval and clustering Fri 21 Jun 2019 13:58:01 AEST ]]> Efficiacy and understanding of the safety hierarchy of controls Fri 09 Nov 2018 12:01:07 AEDT ]]> Epigenetic modifications in essential hypertension Fri 09 Nov 2018 11:40:12 AEDT ]]> Spatial epidemiological investigation of sport and leisure injuries in Victoria, Australia Fri 09 Nov 2018 09:27:09 AEDT ]]> A conceptual framework for a theory of liquidity Fri 05 Apr 2019 15:17:51 AEDT ]]>