- Title
- Denaturation and physical characteristics of spray-dried whey protein isolate powders produced in the presence and absence of Lactose, Trehalose, and Polysorbate-80
- Creator
- Haque, M. Amdadul; Chen, Jie; Aldred, Peter; Adhikari, Benu
- Date
- 2015
- Type
- Text; Journal article
- Identifier
- http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/96716
- Identifier
- vital:10148
- Identifier
-
https://doi.org/10.1080/07373937.2015.1023311
- Identifier
- ISSN:0737-3937
- Abstract
- The denaturation (loss of protein through aggregation and/or change in secondary structure) and physical characteristics such as powder morphology, particle size and size distribution, amorphous/crystalline behavior, and solubility of whey protein isolate (WPI) were investigated in a spray-drying process. The protective efficacy of sugars (lactose and trehalose) and low-molecular-weight surfactant polysorbate-80 (Tween-80) on the secondary structure (-turn, -sheet and -helix) and physical characteristics of spray-dried WPI was quantified. The WPI, WPI+sugar, and WPI+Tween-80 formulations were spray dried maintaining the total solids at 10% (w/w). The inlet and outlet temperatures were maintained at 180 and 80 degrees C, respectively. The results showed that the loss of protein through denaturation and aggregation was not significant (p>0.05). However, a significant (p<0.05) alteration of the secondary structural elements was observed. Due to spray drying of WPI without protectants, the -sheet and -turn were decreased by 4.4 and 14.5%, respectively, and the random coil increased by 20.7%. The -helix of WPI remained unaltered during the spray-drying process. The presence of Tween-80 effectively protected the -helix and -sheet but the -turn remained vulnerable and was decreased. No significant (p>0.05) change in the solubility of WPI was observed due to spray drying. Spray drying of WPI+sugar produced essentially amorphous particles. The dried powder particles were spherical with wrinkled or folded surface.
- Publisher
- Taylor & Francis
- Relation
- Drying Technology Vol. 33, no. 10 (2015), p. 1243-1254
- Rights
- Copyright (C) Taylor & Francis
- Rights
- This metadata is freely available under a CCO license
- Subject
- 0904 Chemical Engineering; Denaturation; Secondary structure; Sugar; Surface morphology; Surfactant; Whey protein isolate
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