The quantitative analysis of thiamin and riboflavin and their respective vitamers in fermented alcoholic beverages

Title: The quantitative analysis of thiamin and riboflavin and their respective vitamers in fermented alcoholic beverages
Creator: Hucker, Barry; Wakeling, Lara; Vriesekoop, Frank
Date: 2011
Type: Text; Journal article
Identifier: http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/56935
Identifier: vital:4547
Identifier: https://doi.org/10.1021/jf202647x
Identifier: ISSN:0021-8561
Abstract: This research aimed to develop a simple and effective method for analyzing thiamin (B 1), riboflavin (B 2) and their respective vitamers by high performance liquid chromatography (HPLC) in fermented alcoholic beverages. The method developed here employs a phosphate buffer/methanol gradient elution on a single reverse phase column, coupled with independent fluorescent detection regimes. It also employs a precolumn derivatization to convert thiamin to thiochrome via an alkaline potassium ferricyanide solution. The method described here allowed a spike recovery of better than 97%, with a typical linear detection range (R 2 ≥ 0.9997) between ≥5 and ≥500
Relation: Journal of Agricultural and Food Chemistry Vol. 59, no. 23 (2011), p. 12278-12285
Rights: American Chemical Society
Rights: This metadata is freely available under a CCO license
Subject: Beer; HPLC; Precolumn derivatization; Riboflavin; Thiamin; Vitamers; High performance liquid chromatography; Potassium; Beverages
Reviewed

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