- Title
- An examination of peripheral blood to reflect transcriptomic adaptation to physical exercise training in sedentary men compared with sex-matched athletic phenotypes
- Creator
- Marin, Sergio
- Date
- 2021
- Type
- Text; Thesis; PhD
- Identifier
- http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/182594
- Identifier
- vital:16145
- Abstract
- There is renewed interest in exercise genomics that peripheral blood RNA expression may be important to understand exercise mediated adaptations to exercise. However, there is little direct supporting evidence. Therefore, this thesis involved two studies to examine the relationship between RNA expression and exercise, and two experimental studies to examine relationships and adaptive response of peripheral blood RNA in sedentary compared with athletic phenotypes. In the first two studies, we conducted both meta-analysis and network meta-analysis to examine current randomised controlled trial (RCT) evidence to determine current best evidence on the link between RNA expression and athletic phenotype in addition to head-to-head comparison of different exercise types to induce differential expression of RNA transcripts in sedentary compared with athletic phenotypes. We observed that current available body of RCTs in peripheral blood exercise genomics presents too large heterogeneity in study design, methodological and data reporting aspects. Thus, we concluded that peripheral blood cannot be established as a valid source for identifying, neither the effect of physical exercise training on transcriptomic markers nor the distinction of divergent transcriptomic profiles in response to different exercise modalities. In the third and fourth studies, we aimed to determine whether peripheral blood RNA and circular RNA (circRNA) expression was different between sedentary and athletic phenotypes, and whether these transcripts were consistent in response to exercise training in exercise-naïve men. To achieve this, we conducted two STROBE compliant observational experiments of n=71 participants with distinct athletic phenotypes. We concluded that peripheral blood transcriptome expression might allow for identification of divergent athletic phenotypes, although this is not supported by further examination of peripheral blood RNA expression levels in response to an exercise training intervention. The sum of works presented in this thesis does not agree with many propositions relating to the strength of evidence in peripheral blood transcriptomics literature. This is principally due to the heterogeneity and lack of consistency of research in this field which is currently insufficient to provide any strong conclusions. In conclusion, peripheral blood RNA and circRNA do not yet offer useful avenues to predict the adaptive response to different exercise types in athletic and non-athletic men.; Doctor of Philosophy
- Publisher
- Federation University Australia
- Rights
- All metadata describing materials held in, or linked to, the repository is freely available under a CC0 licence
- Rights
- Copyright Sergio Marin
- Rights
- Open Access
- Subject
- Physical exercise; Transcriptomics; Gene expression; Peripheral blood
- Full Text
- Thesis Supervisor
- Grace, Fergal
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