Identification of tumor antigens in ovarian cancers using local and circulating tumor‐specific antibodies

- Duarte, Jessica, Quigley, Luke, Young, Anna, Hayashi, Masaru, Meeusen, Els

Title
Identification of tumor antigens in ovarian cancers using local and circulating tumor‐specific antibodies
Creator
Duarte, Jessica; Quigley, Luke; Young, Anna; Hayashi, Masaru; Meeusen, Els
Date
2021
Type
Text; Journal article
Identifier
http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/180609
Identifier
vital:15768
Identifier
https://doi.org/10.3390/ijms222011220
Identifier
ISBN:1661-6596 (ISSN)
Abstract
Ovarian cancers include several disease subtypes and patients often present with advanced metastatic disease and a poor prognosis. New biomarkers for early diagnosis and targeted therapy are, therefore, urgently required. This study uses antibodies produced locally in tumor-draining lymph nodes (ASC probes) of individual ovarian cancer patients to screen two separate protein microarray platforms and identify cognate tumor antigens. The resulting antigen profiles were unique for each individual cancer patient and were used to generate a 50‐antigen custom mi-croarray. Serum from a separate cohort of ovarian cancer patients encompassing four disease sub-types was screened on the custom array and we identified 28.8% of all ovarian cancers, with a higher sensitivity for mucinous (50.0%) and serous (40.0%) subtypes. Combining local and circulating antibodies with high‐density protein microarrays can identify novel, patient‐specific tumor‐associated antigens that may have diagnostic, prognostic or therapeutic uses in ovarian cancer. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. **Please note that there are multiple authors for this article therefore only the name of the first 5 including Federation University Australia affiliate “Els Meeusen” is provided in this record**
Publisher
MDPI
Relation
International Journal of Molecular Sciences Vol. 22, no. 20 (2021), p.
Rights
All metadata describing materials held in, or linked to, the repository is freely available under a CC0 licence
Rights
https://creativecommons.org/licenses/by/4.0/
Rights
Copyright © 2021 by the authors
Rights
Open Access
Subject
0399 Other Chemical Sciences; 0604 Genetics; 0699 Other Biological Sciences; Antibody‐secreting B cells; Biomarkers; Circulating antibodies; Diagnosis; Ovarian cancer; Protein microarrays
Full Text
Reviewed
Funder
This research was funded by the Global Connections Fund—a project run by the Austral‐ ian Academy of Technology and Engineering and supported by the Australian Government—and the Williamstown Rotary Club, Victoria 3016, Australia. The Olivia Newton‐John Cancer Research Institute acknowledges the support of the Victorian Government Operational Infrastructure Sup‐ port Program. J.D.G.D. is supported by Cure Cancer Australia through the Cancer Australia Prior‐ ity‐Driven Cancer Research Scheme (#1187815). The contents of the published material are solely the responsibility of La Trobe University and do not reflect the views of Cancer Australia. A.B. is supported by a fellowship from the Department of Health and Human Services, acting through the Victorian Cancer Agency (VCA).
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