A three-stage intrathymic development pathway for the mucosal-associated invariant T cell lineage
- Koay, Hui-Fern, Gherardin, Nicholas, Enders, Anselm, Loh, Liyen, Mackay, Laura, Almeida, Catarina, Russ, Brendan, Nold-Petry, Claudia, Nold, Marcel, Bedoui, Sammy, Chen, Zhenjun, Corbett, Alexandra, Eckle, Sidonia, Meehan, Bronwyn, d'Udekem, Yves, Konstantinov, Igor, Lappas, Martha, Liu, Ligong, Goodnow, Chris, Fairlie, David, Rossjohn, Jamie, Chong, Mark, Kedzierska, Katherine, Berzins, Stuart, Belz, Gabrielle, McCluskey, James, Uldrich, Adam, Godfrey, Dale, Pellicci, Daniel
- Authors: Koay, Hui-Fern , Gherardin, Nicholas , Enders, Anselm , Loh, Liyen , Mackay, Laura , Almeida, Catarina , Russ, Brendan , Nold-Petry, Claudia , Nold, Marcel , Bedoui, Sammy , Chen, Zhenjun , Corbett, Alexandra , Eckle, Sidonia , Meehan, Bronwyn , d'Udekem, Yves , Konstantinov, Igor , Lappas, Martha , Liu, Ligong , Goodnow, Chris , Fairlie, David , Rossjohn, Jamie , Chong, Mark , Kedzierska, Katherine , Berzins, Stuart , Belz, Gabrielle , McCluskey, James , Uldrich, Adam , Godfrey, Dale , Pellicci, Daniel
- Date: 2016
- Type: Text , Journal article
- Relation: Nature Immunology Vol. 17, no. 11 (2016), p. 1300-1311
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- Reviewed:
- Description: Mucosal-associated invariant T cells (MAIT cells) detect microbial vitamin B2 derivatives presented by the antigen-presenting molecule MR1. Here we defined three developmental stages and checkpoints for the MAIT cell lineage in humans and mice. Stage 1 and stage 2 MAIT cells predominated in thymus, while stage 3 cells progressively increased in abundance extrathymically. Transition through each checkpoint was regulated by MR1, whereas the final checkpoint that generated mature functional MAIT cells was controlled by multiple factors, including the transcription factor PLZF and microbial colonization. Furthermore, stage 3 MAIT cell populations were expanded in mice deficient in the antigen-presenting molecule CD1d, suggestive of a niche shared by MAIT cells and natural killer T cells (NKT cells). Accordingly, this study maps the developmental pathway and checkpoints that control the generation of functional MAIT cells.
- Authors: Koay, Hui-Fern , Gherardin, Nicholas , Enders, Anselm , Loh, Liyen , Mackay, Laura , Almeida, Catarina , Russ, Brendan , Nold-Petry, Claudia , Nold, Marcel , Bedoui, Sammy , Chen, Zhenjun , Corbett, Alexandra , Eckle, Sidonia , Meehan, Bronwyn , d'Udekem, Yves , Konstantinov, Igor , Lappas, Martha , Liu, Ligong , Goodnow, Chris , Fairlie, David , Rossjohn, Jamie , Chong, Mark , Kedzierska, Katherine , Berzins, Stuart , Belz, Gabrielle , McCluskey, James , Uldrich, Adam , Godfrey, Dale , Pellicci, Daniel
- Date: 2016
- Type: Text , Journal article
- Relation: Nature Immunology Vol. 17, no. 11 (2016), p. 1300-1311
- Full Text:
- Reviewed:
- Description: Mucosal-associated invariant T cells (MAIT cells) detect microbial vitamin B2 derivatives presented by the antigen-presenting molecule MR1. Here we defined three developmental stages and checkpoints for the MAIT cell lineage in humans and mice. Stage 1 and stage 2 MAIT cells predominated in thymus, while stage 3 cells progressively increased in abundance extrathymically. Transition through each checkpoint was regulated by MR1, whereas the final checkpoint that generated mature functional MAIT cells was controlled by multiple factors, including the transcription factor PLZF and microbial colonization. Furthermore, stage 3 MAIT cell populations were expanded in mice deficient in the antigen-presenting molecule CD1d, suggestive of a niche shared by MAIT cells and natural killer T cells (NKT cells). Accordingly, this study maps the developmental pathway and checkpoints that control the generation of functional MAIT cells.
An acute bout of static stretching : Effects on force and jumping performance
- Power, Kevin, Behm, David, Cahill, Farrell, Carroll, Michael, Young, Warren
- Authors: Power, Kevin , Behm, David , Cahill, Farrell , Carroll, Michael , Young, Warren
- Date: 2004
- Type: Text , Journal article
- Relation: Medicine and Science in Sports and Exercise Vol. 36, no. 8 (2004), p. 1389-1396
- Full Text: false
- Reviewed:
- Description: Introduction/Purpose: The objectives of this study were to examine whether a static stretching (SS) routine decreased isometric force, muscle activation, and jump power while improving range of motion (ROM). Second, the study attempted to compare the duration of the dependent variable changes with the duration of the change in ROM. Methods: Twelve participants were tested pre- and post- (POST, 30, 60, 90, and 120 min) SS of the quadriceps and plantar flexors (PF) or a similar period of no stretch (control). Measurements during isometric contractions included maximal voluntary force (MVC), evoked contractile properties (peak twitch and tetanus), surface integrated electromyographic (iEMG) activity of the agonist and antagonistic muscle groups, and muscle inactivation as measured by the interpolated twitch technique (ITT). Vertical jump (VJ) measurements included unilateral concentric-only (no countermovement) jump height as well as drop jump height and contact time. ROM associated with seated hip flexion, prone hip extension, and plantar flexion-dorsiflexion was also recorded. Results: After SS, there were significant overall 9.5% and 5.4% decrements in the torque or force of the quadriceps for MVC and ITT, respectively. Force remained significantly decreased for 120 min (10.4%), paralleling significant percentage increases (6%) in sit and reach ROM (120 min). After SS, there were no significant changes in jump performance or PF measures. Conclusion: The parallel duration of changes in ROM and quadriceps isometric force might suggest an association between stretch-induced changes in muscle compliance and isometric force output.
- Description: C1
- Description: 2003001185
Creative activation of the past: Mechanics' Institutes, GLAM, heritage, and creativity in the twenty-first century
- Authors: Tsilemanis, Amy
- Date: 2020
- Type: Text , Thesis , PhD
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- Description: This is an interdisciplinary, mixed-method thesis that explores contemporary curation as a means to creatively activate heritage collections and places. The central case study is Ballaarat Mechanics’ Institute (BMI), in Ballarat, Australia, where practice and action-led research was undertaken by the curator over the three-year period 2016–2019. Creative connections between five interlinked areas are critically examined: heritage; curatorial practice, by which heritage sites, collections and experiences are managed; historic cultural organisations; their city contexts; and the ways in which such cultural work is valued. The framework for analysis encompasses museology, critical heritage, and approaches to cultural value. Contemporary urban Mechanics’ Institutes (MIs) are placed in the museum context both through historic parallels and their contemporary positioning in the GLAM (galleries, libraries, archives and museums) industry sector. This is in order to ask how heritage collections, and the organisations that house and present them, can creatively connect with the publics they serve with greater connectivity and relevance. Exhibitions and events held at BMI within Ballarat city are treated as case studies. Together with qualitative interviews with staff in the Ballarat GLAM sector and urban MIs, insights derived illuminate the role and challenges of such cultural organisations in the twenty-first century. It is argued that, when employing the practice and energy of the curator, creative activations have the potential to open new points of entry to, and provide alternative perspectives upon, heritage places and collections. This is achieved through arts practice, organisational thinking, and bringing to life the links between past, present and future. In this process, new and dynamic measures of value can be explored and create dialogic encounters between people, heritage and ideas.
- Description: Doctor of Philosophy
- Authors: Tsilemanis, Amy
- Date: 2020
- Type: Text , Thesis , PhD
- Full Text:
- Description: This is an interdisciplinary, mixed-method thesis that explores contemporary curation as a means to creatively activate heritage collections and places. The central case study is Ballaarat Mechanics’ Institute (BMI), in Ballarat, Australia, where practice and action-led research was undertaken by the curator over the three-year period 2016–2019. Creative connections between five interlinked areas are critically examined: heritage; curatorial practice, by which heritage sites, collections and experiences are managed; historic cultural organisations; their city contexts; and the ways in which such cultural work is valued. The framework for analysis encompasses museology, critical heritage, and approaches to cultural value. Contemporary urban Mechanics’ Institutes (MIs) are placed in the museum context both through historic parallels and their contemporary positioning in the GLAM (galleries, libraries, archives and museums) industry sector. This is in order to ask how heritage collections, and the organisations that house and present them, can creatively connect with the publics they serve with greater connectivity and relevance. Exhibitions and events held at BMI within Ballarat city are treated as case studies. Together with qualitative interviews with staff in the Ballarat GLAM sector and urban MIs, insights derived illuminate the role and challenges of such cultural organisations in the twenty-first century. It is argued that, when employing the practice and energy of the curator, creative activations have the potential to open new points of entry to, and provide alternative perspectives upon, heritage places and collections. This is achieved through arts practice, organisational thinking, and bringing to life the links between past, present and future. In this process, new and dynamic measures of value can be explored and create dialogic encounters between people, heritage and ideas.
- Description: Doctor of Philosophy
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