- Title
- Relationship between anti-DFS70 autoantibodies and oxidative stress
- Creator
- Krzemień, Pawel; Kasperczyk, Slawomir; Banach, Maciej; Kasperczyk, Aleksandra; Dobrakowski, Michal; Tomasik, Tomasz; Windak, Adam; Mastej, Miroslaw; Catapano, Alberico; Ray, Kausik; Mikhailidis, Dimitri; Toth, Peter; Howard, George; Lip, Gregory; Tomaszewski, Maciej; Charchar, Fadi; Sattar, Naveed; Williams, Bryan; MacDonald, Thomas; Penson, Peter; Jóźwiak, J; Al-Shaer, B.; Andrusewicz, W.; Andrzejczuk-Rosa, M.; Anusz-Gaszewska, E.; Bagińska, A.; Balawajder, P.; Bańka, G.; Barańska-Skubisz, E.; Barbara Przyczyna, B.
- Date
- 2022
- Type
- Text; Journal article
- Identifier
- http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/187799
- Identifier
- vital:17150
- Identifier
-
https://doi.org/10.1177/11772719211066791
- Identifier
- ISSN:1177-2719 (ISSN)
- Abstract
- Background: The anti-DFS70 autoantibodies are one of the most commonly and widely described agent of unknown clinical significance, frequently detected in healthy individuals. It is not known whether the DFS70 autoantibodies are protective or pathogenic. One of the factors suspected of inducing the formation of anti-DFS70 antibodies is increased oxidative stress. We evaluated the coexistence of anti-DFS70 antibodies with selected markers of oxidative stress and investigated whether these antibodies could be considered as indirect markers of oxidative stress. Methods: The intensity of oxidative stress was measured in all samples via indices of free-radical damage to lipids and proteins such as total oxidant status (TOS), concentrations of lipid hydroperoxides (LPH), lipofuscin (LPS), and malondialdehyde (MDA). The parameters of the non-enzymatic antioxidant system, such as total antioxidant status (TAS) and uric acid concentration (UA), were also measured, as well as the activity of superoxide dismutase (SOD). Based on TOS and TAS values, the oxidative stress index (OSI) was calculated. All samples were also tested with indirect immunofluorescence assay (IFA) and 357 samples were selected for direct monospecific anti DFS70 enzyme-linked immunosorbent assay (ELISA) testing. Results: The anti-DFS70 antibodies were confirmed by ELISA test in 21.29% of samples. Compared with anti-DFS70 negative samples we observed 23% lower concentration of LPH (P =.038) and 11% lower concentration of UA (P =.005). TOS was 20% lower (P =.014). The activity of SOD was up to 5% higher (P =.037). The Pearson correlation showed weak negative correlation for LPH, UA, and TOS and a weak positive correlation for SOD activity. Conclusion: In samples positive for the anti-DFS70 antibody a decreased level of oxidative stress was observed, especially in the case of samples with a high antibody titer. Anti-DFS70 antibodies can be considered as an indirect marker of reduced oxidative stress or a marker indicating the recent intensification of antioxidant processes. © The Author(s) 2022. **Please note that there are multiple authors for this article therefore only the name of the first 30 including Federation University Australia affiliate “Fadi Charchar” is provided in this record**
- Publisher
- SAGE Publications Ltd
- Relation
- Biomarker Insights Vol. 17, no. (2022), p.
- Rights
- All metadata describing materials held in, or linked to, the repository is freely available under a CC0 licence
- Rights
- https://creativecommons.org/licenses/by-nc/4.0/
- Rights
- Copyright © The Author(s) 2022
- Rights
- Open Access
- Subject
- 3205 Medical biochemistry and metabolomics; 3214 Pharmacology and pharmaceutical sciences; Antibodies; Biomarkers; Free radicals; Oxidative stress
- Full Text
- Reviewed
- Funder
- The present study was an initiative of the Polish Lipid Association (PoLA) and the College of Family Physician in Poland (CFPiP). The present study was funded by an unrestricted educational grant from Valeant (Warsaw, Poland). Valeant had no role in study design, data analysis, data interpretation, or writing of the report. The present study was also supported by Silesian Analytical Laboratories (SLA), CHDE, BIO-RAD, and Euroimmun. The study was funded by the Medical University of Silesia, grant no. PCN-2-001/N/1/O.
- Hits: 4006
- Visitors: 3312
- Downloads: 69
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | Published version | 499 KB | Adobe Acrobat PDF | View Details Download |