- Title
- Evaluation of the role of galectins in parasite immunity
- Creator
- Preston, Sarah; Dunphy, Jillian; Beddoe, Travis; Meeusen, Els; Young, Anna
- Date
- 2014
- Type
- Text; Book chapter
- Identifier
- http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/163105
- Identifier
- vital:12782
- Identifier
-
https://doi.org/10.1007/978-1-4939-1396-1_25
- Identifier
- ISBN:1064-3745 (ISSN); 978-1-4939-1396-1 (ISBN)
- Abstract
- Galectin-11 and galectin-14 are ruminant galectins involved in parasitic infections. Although their roles in parasite immunity are still being elucidated, its appears that their functions are parasite specific. In gastrointestinal infections with the nematode Haemonchus contortus, both galectin-11 and galectin-14 appear to be protective. However, in a chronic infection of liver fluke, Fasciola hepatica, these galectins may aid parasite survival. This chapter discusses the methods designed to study parasitic infections in sheep, which have provided us with insight into the functions of galectin-11 and galectin-14 during host–parasite interactions. These methods include parasite cultivation and infection, galectin staining of host and parasite tissue, surface staining of parasites with recombinant galectins and in vitro assays to monitor the effect of galectins on larval development. © Springer Science+Business Media New York 2015.
- Publisher
- Humana Press Inc.
- Relation
- Galectins: Methods and Protocols (Methods in Molecular Biology series) Chapter 25 p. 371-395
- Rights
- Copyright © Springer Science+Business Media New York 2015.
- Rights
- This metadata is freely available under a CCO license
- Subject
- Fasciola hepatica; Galectin-11; Galectin-14; Haemonchus contortus; Immunohistochemistry; Larval culture; Larval feeding assay; Larval growth assay; Larval molting assay
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