Description:
The negative-sense asp open reading frame (ORF) positioned opposite to the human immunodeficiency virus type 1 (HIV-1) env gene encodes the 189 amino acid, membrane-associated ASP protein. Negative-sense transcription, regulated by long terminal repeat sequences, has been observed early in HIV-1 infection in vitro. All subtypes of HIV-1 were scanned to detect the negative-sense asp ORF and to identify potential regulatory sequences. A series of highly conserved upstream short open reading frames (sORFs) was identified. This potential control region from HIV-1NL4-3, containing six sORFs, was cloned upstream of the reporter gene EGFP. Expression by transfection of HEK293 cells indicated that the introduction of this sORF region inhibits EGFP reporter expression; analysis of transcripts revealed no significant changes in levels of EGFP mRNA. Reverse transcriptase–polymerase chain reaction analysis (RT-PCR) further demonstrated that the upstream sORF region undergoes alternative splicing in vitro. The most abundant product is spliced to remove sORFs I to V, leaving only the in-frame sORF VI upstream of asp. Sequence analysis revealed the presence of typical splice donor- and acceptor-site motifs. Mutation of the highly conserved splice donor and acceptor sites modulates, but does not fully relieve, inhibition of EGFP production. The strong conservation of asp and its sORFs across all HIV-1 subtypes suggests that the asp gene product may have a role in the pathogenesis of HIV-1. Alternative splicing of the upstream sORF region provides a potential mechanism for controlling expression of the asp gene.
Description:
As part of Latrobe City’s community engagement strategy, a survey was developed and distributed during May to June 2021, collecting both quantitative and qualitative data to capture feedback and suggestions on how Latrobe City could be a better place to live, work, visit and play. The survey also invited residents to share their thoughts on current and future challenges for the City. A total of 1552 responses to the survey were received of which 69 were blank, with 1453 making up the final data set. This report highlights the findings from the survey. The majority of the participants were female (65%, n=744), 33% were from Traralgon, 35% from smaller townships with the remaining 32% from Moe, Newborough, Morwell and Churchill. The participants ranged in age from 17-30 years (15%), 31-40 years (27%), 41-70 years (43%) 70+ years (10%). The majority of the participants were employed in healthcare, education and administrative roles with 89% stating that they live and work in Latrobe City. Friends are family was cited as the most common reason why participants believed that Latrobe City was a good place, with Location in Victoria, Affordability and Natural environment rounding off the top four responses. The most common reason people gave for Latrobe City being a good place to work was Distance from home (n=504) and similarly the most common reason participants gave for it being a good place to study was Close to home. Safety, Economy and Built environment were considered the things most important for Latrobe City Council to focus on this was evident in both the quantitative and qualitative responses. A thematic analysis process was used to analyse the content from the six open-ended questions in the survey. Six main themes emerged; Employment, Safety, Transport, Education, Sport and Entertainment and Hope for the future.
Description:
As part of Latrobe City’s community engagement strategy, a survey was developed and distributed during May to June 2021, collecting both quantitative and qualitative data to capture feedback and suggestions on how Latrobe City could be a better place to live, work, visit and play. The survey also invited residents to share their thoughts on current and future challenges for the City. A total of 1552 responses to the survey were received of which 69 were blank, with 1453 making up the final data set. This report highlights the findings from the survey. The majority of the participants were female (65%, n=744), 33% were from Traralgon, 35% from smaller townships with the remaining 32% from Moe, Newborough, Morwell and Churchill. The participants ranged in age from 17-30 years (15%), 31-40 years (27%), 41-70 years (43%) 70+ years (10%). The majority of the participants were employed in healthcare, education and administrative roles with 89% stating that they live and work in Latrobe City. Friends are family was cited as the most common reason why participants believed that Latrobe City was a good place, with Location in Victoria, Affordability and Natural environment rounding off the top four responses. The most common reason people gave for Latrobe City being a good place to work was Distance from home (n=504) and similarly the most common reason participants gave for it being a good place to study was Close to home. Safety, Economy and Built environment were considered the things most important for Latrobe City Council to focus on this was evident in both the quantitative and qualitative responses. A thematic analysis process was used to analyse the content from the six open-ended questions in the survey. Six main themes emerged; Employment, Safety, Transport, Education, Sport and Entertainment and Hope for the future.
Description:
The ‘I Am Ready’ Program was an innovative approach to engaging secondary school students with learning barriers, to think, plan and actively move towards employment and or further education and training. This summary has been divided into five sections which demonstrates the triangulation of the evaluation findings, highlights the key findings and includes; Reach of the program, Lessons learnt, Breaking down barriers, Building confidence and Bright future for participants and the Program.
Description:
The ‘I Am Ready’ Program was an innovative approach to engaging secondary school students with learning barriers, to think, plan and actively move towards employment and or further education and training. This summary has been divided into five sections which demonstrates the triangulation of the evaluation findings, highlights the key findings and includes; Reach of the program, Lessons learnt, Breaking down barriers, Building confidence and Bright future for participants and the Program.
Description:
Reflective practice post clinical placement in nursing education is essential to integrate knowledge, skills, attitudes, and experiences. Many nursing programs in-cooperate reflective practice to permit the integration of core nursing education with clinical experiences to develop safe and component nurses. The purpose of this literature review is to examine the current trends in nursing education around reflective practice post clinical practice experiences.
Description:
Reflective practice post clinical placement in nursing education is essential to integrate knowledge, skills, attitudes, and experiences. Many nursing programs in-cooperate reflective practice to permit the integration of core nursing education with clinical experiences to develop safe and component nurses. The purpose of this literature review is to examine the current trends in nursing education around reflective practice post clinical practice experiences.
Description:
The primary focus of the Yinnar Health and Wellness Program is to improve the health and wellbeing of older adults in the Gippsland region by removing barriers to participation in facilitated health and wellbeing activities. This report provides analysis and evaluation of the impact of the Program using the CEU Participatory Evaluation Framework for the period February – December 2019. Working in collaboration with key stakeholders, a mixed methods approach was taken and included the analysis of both quantitative and qualitative data. A literature review was also completed. Results of the data show that the Program successfully delivered weekly classes for a period of twelve months to a consistent number of participants with some significant positive outcomes. The Program was adaptive in design so that it met the individual needs of each participant. Participants reported improvements in their physical health and in their confidence. Results also showed that the Program had a positive impact on the social connectiveness of participants. In confirmation of the literature that was analysed the Program has shown that regular exercise classes have a positive physical, social, and psychological impact on individuals. Although the majority of the participants lived in Yinnar, there were a number of regulars that travelled to attend the program from neighbouring communities. There was a direct impact to the participant’s health with many individuals stating that their overall health, function and movement had improved because of attending the program. A total of 23 out of 24 participants agreed or strongly agreed that the Program had improved their fitness levels and overall confidence. Participants stated that the Program addressed a gap in the service delivery for the region. Not only did the Program improve recovery from illness it helped to maintain overall fitness levels of the participants which ultimately may lead to fewer hospital admissions. It also addressed the need for organised social connection activities for the aging population. The participants agreed that the Program met the needs of the community and the individuals, providing significant physical, mental and social benefits from regular attendance.
Description:
The primary focus of the Yinnar Health and Wellness Program is to improve the health and wellbeing of older adults in the Gippsland region by removing barriers to participation in facilitated health and wellbeing activities. This report provides analysis and evaluation of the impact of the Program using the CEU Participatory Evaluation Framework for the period February – December 2019. Working in collaboration with key stakeholders, a mixed methods approach was taken and included the analysis of both quantitative and qualitative data. A literature review was also completed. Results of the data show that the Program successfully delivered weekly classes for a period of twelve months to a consistent number of participants with some significant positive outcomes. The Program was adaptive in design so that it met the individual needs of each participant. Participants reported improvements in their physical health and in their confidence. Results also showed that the Program had a positive impact on the social connectiveness of participants. In confirmation of the literature that was analysed the Program has shown that regular exercise classes have a positive physical, social, and psychological impact on individuals. Although the majority of the participants lived in Yinnar, there were a number of regulars that travelled to attend the program from neighbouring communities. There was a direct impact to the participant’s health with many individuals stating that their overall health, function and movement had improved because of attending the program. A total of 23 out of 24 participants agreed or strongly agreed that the Program had improved their fitness levels and overall confidence. Participants stated that the Program addressed a gap in the service delivery for the region. Not only did the Program improve recovery from illness it helped to maintain overall fitness levels of the participants which ultimately may lead to fewer hospital admissions. It also addressed the need for organised social connection activities for the aging population. The participants agreed that the Program met the needs of the community and the individuals, providing significant physical, mental and social benefits from regular attendance.