A novel plasmid-mediated polymyxin resistance determinant (mcr-1.8) in escherichia coli recovered from broiler chickens in Brunei Darussalam
- Authors: Momin, Abdul , Liakopoulos, Apostolos , Bean, David , Phee, Lynette , Wareham, David
- Date: 2019
- Type: Text , Journal article , Letter
- Relation: Journal of Antimicrobial Chemotherapy Vol. 74, no. 11 (2019), p. 3392-3394
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- Description: Sir, MDR Gram-negative bacteria are identified as critical pathogens and their effective treatment increasingly relies on the polymyxins (polymyxin B, colistin), either alone or as part of unorthodox combination therapies.1 The rapid emergence of polymyxin resistance due to mutations/insertions in genes involved in LPS modifications (lpxCAD, pmrA/B, mgrB, phoP/Q, ccrAB) has been reported among individuals exposed to or treated with polymyxins.1Of greater concern are increasing reports of resistance due to the acquisition of phosphoethanolamine (PEtN) transferases, enzymes that catalyse the addition of phosphoethanolamine to lipid A, resulting in lower binding affinity of polymyxins.1 Since the first identification in China,1 multiple gene variants have been reported in diverse bacterial genera recovered from a range of human, retail food, foodproducing animal and environmental sources.2 Here, we report a novel variant of PEtN transferase, designated MCR-1.8, and its genetic context in an MDR Escherichia coli isolate recovered from poultry in Brunei Darussalam.
Singlet molecular oxygen regulates vascular tone and blood pressure in inflammation
- Authors: Stanley, Christopher , Maghzal, Ghassan , Ayer, Anita , Talib, Jihan , Giltrap, Andrew , Shengule, Sudhir , Wolhuter, Kathryn , Wang, Yutang , Chadha, Preet , Suarna, Cacang , Prysyazhna, Oleksandra , Scotcher, Jenna , Dunn, Louise , Prado, Fernanda , Nguyen, Nghi , Odiba, Jephthah , Baell, Johathan , Stasch, Johannes-Peter , Yamamoto, Yorihiro , Di Mascio, Paolo , Eaton, Philip , Payne, Richard , Stocker, Roland
- Date: 2019
- Type: Text , Journal article , Letter
- Relation: Nature Vol. 566, no. 7745 (2019), p. 548-552
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- Description: Singlet molecular oxygen (O-1(2)) has well-established roles in photosynthetic plants, bacteria and fungi(1-3), but not in mammals. Chemically generated O-1(2) oxidizes the amino acid tryptophan to precursors of a key metabolite called N-formylkynurenine(4), whereas enzymatic oxidation of tryptophan to N-formylkynurenine is catalysed by a family of dioxygenases, including indoleamine 2,3-dioxygenase 1(5). Under inflammatory conditions, this haem-containing enzyme is expressed in arterial endothelial cells, where it contributes to the regulation of blood pressure(6). However, whether indoleamine 2,3-dioxygenase 1 forms O-1(2) and whether this contributes to blood pressure control have remained unknown. Here we show that arterial indoleamine 2,3-dioxygenase 1 regulates blood pressure via formation of O-1(2). We observed that in the presence of hydrogen peroxide, the enzyme generates O-1(2) and that this is associated with the stereoselective oxidation of L-tryptophan to a tricyclic hydroperoxide via a previously unrecognized oxidative activation of the dioxygenase activity. The tryptophan-derived hydroperoxide acts in vivo as a signalling molecule, inducing arterial relaxation and decreasing blood pressure; this activity is dependent on Cys42 of protein kinase G1 alpha. Our findings demonstrate a pathophysiological role for O-1(2) in mammals through formation of an amino acid-derived hydroperoxide that regulates vascular tone and blood pressure under inflammatory conditions.
Comment : Translating guidelines into practice : Interpreting the 2016 ACC expert consensus decision pathway on the role of non-statin therapies for LDL cholesterol lowering in the management of atherosclerotic cardiovascular disease risk
- Authors: Steicke, Michelle , Wang, Yutang
- Date: 2018
- Type: Text , Journal article , Letter
- Relation: Annals of Pharmacotherapy Vol. 52, no. 1 (2018), p. 91
- Relation: http://purl.org/au-research/grants/nhmrc/1062671
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Letter by Wang regarding article, "Efficacy and safety of catheter-based radiofrequency renal denervation in stented renal arteries"
- Authors: Wang, Yutang
- Date: 2015
- Type: Text , Journal article , Letter
- Relation: Circulation: Cardiovascular Interventions Vol. 8, no. 2 (2015), p.1
- Relation: http://purl.org/au-research/grants/nhmrc/1062671
- Full Text: false
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Ambulatory blood pressure may be designed as the primary efficacy outcome in clinical trials on renal denervation
- Authors: Wang, Yutang
- Date: 2014
- Type: Text , Letter , Journal article
- Relation: International Journal of Cardiology Vol. 176, no. 3 (2014), p. 1262-1263
- Relation: http://purl.org/au-research/grants/nhmrc/1062671
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It is urgent to investigate predictors of the response of blood pressure to renal denervation
- Authors: Wang, Yutang
- Date: 2014
- Type: Text , Journal article , Letter
- Relation: Canadian Journal of Cardiology Vol. 30, no. 4 (2014), p. 465.e7
- Relation: http://purl.org/au-research/grants/nhmrc/1062671
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It may be not suitable to perform renal denervation in renal arteries with significant stenosis
- Authors: Wang, Yutang
- Date: 2014
- Type: Text , Journal article , Letter
- Relation: International Journal of Cardiology Vol. 174, no. 3 (2014), p. 750
- Relation: http://purl.org/au-research/grants/nhmrc/1062671
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Limitations in current clinical trials on renal denervation
- Authors: Wang, Yutang
- Date: 2014
- Type: Text , Journal article , Letter
- Relation: International Journal of Cardiology Vol. 174, no. 1 (2014), p. 225
- Relation: http://purl.org/au-research/grants/nhmrc/1062671
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Single-sided renal denervation may be not suitable for patients with significant renal artery stenosis
- Authors: Wang, Yutang
- Date: 2014
- Type: Text , Journal article , Letter
- Relation: Clinical Research in Cardiology Vol. 103, no. 11 (2014), p. 950-951
- Relation: http://purl.org/au-research/grants/nhmrc/1062671
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