Physicochemical and functional properties of lentil protein isolates prepared by different drying methods
- Authors: Joshi, Matina , Adhikari, Benu , Aldred, Peter , Panozzo, Joe , Kasapis, Stefan
- Date: 2011
- Type: Text , Journal article
- Relation: Food Chemistry Vol. 129, no. 4 (2011), p. 1513-1522
- Full Text: false
- Description: Lentil protein isolate (LPI) extract was converted into powder by freeze drying, spray drying and vacuum drying. Differences in particle size distribution, protein subunit composition and colour and surface morphology were observed amongst the three drying methods. Spray and freeze-dried LPI powders exhibited higher solubility (81% and 78%, respectively) compared to vacuum dried powders (50%). The spray dried powders showed a low water absorption capacity (0.43 ± 0.02 g/g) compared to freeze (0.48 ± 0.02 g/g) and vacuum-dried (0.47 ± 0.01 g/g) LPI powders. Spray and freeze-dried powders displayed better gelation ability and higher gel strength, compared to vacuum-dried powder. Both spray and freeze-dried gels showed typical viscoelastic gel characteristics, with G′ dominating over G″ and very low loss tangent. The holding time required for gelation of vacuum dried powder at 90 °C was significantly longer, compared to spray and freeze dried powders. Hence, drying methods used for preparation of lentil protein isolate powders can affect physicochemical and associated functional properties. © 2011 Elsevier Ltd. All rights reserved.
Survival, fermentation activity and storage stability of spray dried Lactococcus lactis produced via different atomization regimes
- Authors: Ghandi, Amir , Powell, Ian , Broome, Melcolm , Adhikari, Benu
- Date: 2013
- Type: Text , Journal article
- Relation: Journal of Food Engineering Vol. 115, no. 1 (2013), p. 83-90
- Full Text:
- Description: Dried powders containing Lactococcus lactis ssp. cremoris were produced using laboratory and pilot scale spray dryers with lactose:whey protein isolate (3:1) as a protective medium. The effects of storage temperature (25, 4 and -18 Â°C) and time (30, 60 and 90 days) were studied. The survival and fermentation activity of the dried bacterial cells were significantly lower when the powders were stored at 25 Â°C compared to those stored at 4 and -18 Â°C; powders stored at 4 and -18 Â°C were statistically similar. The survival and fermentation activity of bacterial cells obtained from a laboratory scale two-fluid nozzle spray dryer were found to be higher than those of cells obtained from a pilot scale two-fluid spray dryer. A rotary wheel atomizer gave significantly higher survival and activity in the same dryer. These observations are consistent with cell damage due to high characteristic shear rates in the atomization process in nozzle type atomizers. The presence of ascorbic acid (oxygen scavenger) in the powder composition was found to improve both the survival and the maintenance of fermentation activity of the dried bacterial cells significantly during storage. The survival and fermentation activity of dried bacterial cells in stored powders indicated that these parameters are system-specific and can be strongly affected by the storage temperature and presence or absence of antioxidant, and also by upstream processing conditions such as the mode of atomization and presence or absence of antioxidants in the dryer feed. © 2012 Elsevier Ltd. All rights reserved.
- Description: 2003010581