Effect of environmental factors on the germination and emergence of Salvia verbenaca L. cultivars (verbenaca and vernalis) : An invasive species in semi-arid and arid rangeland regions
- Authors: Javaid, Muhammad , Florentine, Singarayer , Ali, Hafiz , Weller, Sandra
- Date: 2018
- Type: Text , Journal article
- Relation: PLoS ONE Vol. 13, no. 3 (2018), p. 1-20
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- Description: Salvia verbenaca (wild sage) is a commonly cultivated herbal medicine plant, which is native to the Mediterranean climate regions of Europe, Africa, Asia and the Middle East. However, it has become an invasive species in semi-arid and arid regions of southern Australia. Two varieties are present in this region, var. verbenaca and var. vernalis, each of which can be distinguished by differences in morphology and flowering period. Following trials to determine the optimum temperate regime for germination and response to light and dark, seeds of both varieties were tested for their response to variations in pH, moisture stress, salinity, and burial depth. The temperature and light trial was carried out using three different temperature regimes; 30/20C, 25/15C and 20/12C, and two light regimes; 12 hours light/12 hours dark and 24 hours dark, with var. vernalis responding to relatively higher temperatures than var. verbenaca. The germination rate of neither species was significantly inhibited by complete darkness when compared to rates under periodic light exposure. Both varieties germinated at near optimum rates strongly to very strongly in all pH buffer solutions, from pH 5 to pH 10, but they responded most strongly at neutral pH. Var. vernalis showed slightly more tolerance to reduced moisture availability, moderate to strong salinity, and burial depth, compared to var. verbenaca. However, even a fairly shallow burial depth of 2 cm completely inhibited germination of both varieties. Thus, in circumstances where both varieties are present in a soil seedbank, var. vernalis could be expected to establish in more challenging conditions, where moisture is limited and salinity is ‘moderate to high’, implying that it is a more serious threat for invasive weed in conditions where crop plants are already challenged.
Polyclonal T-Cells Express CD1a in Langerhans Cell Histiocytosis (LCH) Lesions
- Authors: West, Jennifer , Olsen, Sharon , Mitchell, Jenée , Priddle, Ross , Luke, Jennifer , Åkefeldt, Selma Olsson , Henter, Jan-Inge , Turville, Christopher , Kannourakis, George
- Date: 2014
- Type: Text , Journal article
- Relation: PLoS ONE Vol. 9, no. 10 (2014), p. 1-12
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- Description: Langerhans cell histiocytosis (LCH) is a complex and poorly understood disorder that has characteristics of both inflammatory and neoplastic disease. By using eight-colour flow cytometry, we have identified a previously unreported population of CD1a+/CD3+ T-cells in LCH lesions. The expression of CD1a is regarded as a hallmark of this disease; however, it has always been presumed that it was only expressed by pathogenic Langerhans cells (LCs). We have now detected CD1a expression by a range of T-cell subsets within all of the LCH lesions that were examined, establishing that CD1a expression in these lesions is no longer restricted to pathogenic LCs. The presence of CD1a+ T-cells in all of the LCH lesions that we have studied to date warrants further investigation into their biological function to determine whether these cells are important in the pathogenesis of LCH. [ABSTRACT FROM AUTHOR] Copyright of PLoS ONE is the property of Public Library of Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Influence of fasting during moult on the faecal microbiota of penguins
- Authors: Dewar, Meagan , Arnould, John , Krause, Lutz , Trathan, Phil , Dann, Peter , Smith, Stuart
- Date: 2014
- Type: Text , Journal article
- Relation: PLoS ONE Vol. 9, no. 6 (2014), p.
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- Description: Many seabirds including penguins are adapted to long periods of fasting, particularly during parts of the reproductive cycle and during moult. However, the influence of fasting on the gastrointestinal (GI) microbiota has not been investigated in seabirds. Therefore, the present study aimed to examine the microbial composition and diversity of the GI microbiota of fasting little (Eudyptula minor) and king penguins (Aptenodytes patagonicus) penguins during early and late moult. The results from this study indicated that there was little change in the abundance of the major phyla during moult, except for a significant increase in the level of Proteobacteria in king penguins. In king penguins the abundance of Fusobacteria increases from 1.73% during early moult to 33.6% by late moult, whilst the abundance of Proteobacteria (35.7% to 17.2%) and Bacteroidetes (19.5% to 11%) decrease from early to late moult. In little penguins, a decrease in the abundances of Firmicutes (44% to 29%) and an increase in the abundance of Bacteroidetes (11% to 20%) were observed from early to late moult respectively. The results from this study indicate that the microbial composition of both king and little penguins alters during fasting. However, it appears that the microbial composition of king penguins is more affected by fasting than little penguins with the length of fast the most probable cause for this difference.
Detecting the seeds of Nassella neesiana in large round hay bales, by means of non-destructive core sampling
- Authors: Weller, Sandra , Florentine, Singarayer , Sillitoe, Jim , Grech, Charles , McLaren, David , Chauhan, Bhagirath
- Date: 2015
- Type: Text , Journal article
- Relation: Plos One Vol. 10, no. 9 (2015), p.
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- Description: In the last three decades or so there has been a significant increase in fodder trading, both in terms of the quantity of fodder traded and in terms of its economic value to the industry. Often, this fodder type may be supplied free of charge to graziers in distress due to circumstances that follow natural disasters such as bushfires, drought, and flood. However, because of the obvious urgency arising from these situations, it is suspected that much relief fodder may unintentionally pose an elevated risk for dispersal of weeds since it may be supplied from pasture not normally used for trade in fodder, and therefore is of unknown quality. Previous destructive method to detect weed propagules in bales of fodder are cumbersome, time consuming and of limited ecological and statistical value. Therefore, objective of this paper was to development of a convenient method to assess round pasture hay bales for the presence of weed propagules, to prevent unintentional spread of noxious species in hay bales. To examine this objective known quantity of seeds were added in a series of distributions to bales of seed free pasture hay, and a positive correlation for the amount of seed added per bale with that recovered in core samples was observed. Whilst the number of seeds detected per bale varied according to the distribution of seeds within the bales and the number of cores analysed, the absolute detection of seeds suggests that this sampling method is worthy of further examination. In addition, a pragmatic estimation of bale remnants after stock feeding has been investigated to more closely estimate the potential size of the remaining seed bank. The authors propose that development of this approach is timely, in the light of future climatic uncertainty driving extreme weather events that increase the need for relief fodder, which can be a potential vector for the spread of noxious weed seeds.
Fast mode decision in the HEVC Video coding standard by exploiting region with dominated motion and saliency features
- Authors: Podder, Pallab , Paul, Manoranjan , Murshed, Manzur
- Date: 2012
- Type: Text , Journal article
- Relation: PLoS ONE Vol. Vol.11, no. 3 (2012), p. p.e0150673
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- Description: The emerging High Efficiency Video Coding (HEVC) standard introduces a number of innovative and powerful coding tools to acquire better compression efficiency compared to its predecessor H.264. The encoding time complexities have also increased multiple times that is not suitable for realtime video coding applications. To address this limitation, this paper employs a novel coding strategy to reduce the time complexity in HEVC encoder by efficient selection of appropriate block-partitioning modes based on human visual features (HVF). The HVF in the proposed technique comprise with human visual attention modelling-based saliency feature and phase correlation-based motion features. The features are innovatively combined through a fusion process by developing a content-based adaptive weighted cost function to determine the region with dominated motion/saliency (RDMS)- based binary pattern for the current block. The generated binary pattern is then compared with a codebook of predefined binary pattern templates aligned to the HEVC recommended block-paritioning to estimate a subset of inter-prediction modes. Without exhaustive exploration of all modes available in the HEVC standard, only the selected subset of modes are motion estimated and motion compensated for a particular coding unit. The experimental evaluation reveals that the proposed technique notably down-scales the average computational time of the latest HEVC reference encoder by 34% while providing similar rate-distortion (RD) performance for a wide range of video sequences.
Microbiota of little penguins and short-tailed shearwaters during development
- Authors: Dewar, Meagan , Arnould, John , Allnutt, Theo , Crowley, Tamsyn , Krause, Lutz , Reynolds, John , Dann, Peter , Smith, Stuart
- Date: 2017
- Type: Text , Journal article
- Relation: PLoS ONE Vol. 12, no. 8 (2017), p.
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- Description: The establishment and early colonisation of the gastrointestinal (GI) tract has been recognised as a crucial stage in chick development, with pioneering microbial species responsible for influencing the development of the GI tract and influencing host health, fitness and disease status throughout life. Development of the microbiota in long lived seabirds is poorly understood. This study characterised the microbial composition of little penguin and short-tailed shearwater chicks throughout development, using Quantitative Real Time PCR (qPCR) and 16S rRNA sequencing. The results indicated that microbial development differed between the two seabird species with the short-tailed shearwater microbiota being relatively stable throughout development whilst significant fluctuations in the microbial composition and an upward trend in the abundance of Firmicutes and Bacteroidetes were observed in the little penguin. When the microbial composition of adults and chicks was compared, both species showed low similarity in microbial composition, indicating that the adult microbiota may have a negligible influence over the chick’s microbiota.
Influence of soil moisture regimes on growth, photosynthetic capacity, leaf biochemistry and reproductive capabilities of the invasive agronomic weed; Lactuca serriola
- Authors: Chadha, Aakansha , Florentine, Singarayer , Chauhan, Bhagirath , Long, Benjamin , Jayasundera, Mithila
- Date: 2019
- Type: Text , Journal article
- Relation: PLoS ONE Vol. 14, no. 6 (2019), p. 1-17
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- Description: Global temperatures are predicted to increase by 1.5–5.9C during this century, and this change is likely to impact average rainfall, with predictions that water deficit will perhaps be the most severe threat to sustainable agriculture. In this respect, invasive weeds, which have traits better adapted to drought stress than crops, add to concerns regarding crop sustainability. Lactuca serriola, an aggressive agronomic weed is thought to be a successful weed because of its ability to maintain high water use efficiency under drought conditions. In this study, experiments were conducted to examine the influence of different soil moisture regimes (100%, 75%, 50% and 25% water holding capacity (WHC)) on growth, photosynthetic capacity, leaf biochemistry and reproduction of this species. Soil moisture significantly affected plant’s height, stem diameter, number of leaves and biomass. The highest plant height (115.14 cm ± 11.64), shoot diameter (9.4 mm ± 0.18), leaf area (1206.5 mm2 ± 73.29), plant fresh weight (83.1 ± 3.98) and dry weight (22.38 ± 1.24) were recorded at 75% soil moisture content. A fundamental adaptation to drought was observed as plants in the 25% WHC treatment had the highest root: shoot ratio. Soluble sugars and total phenolic content were highest in the 25% WHC treatment and significantly different to 100% WHC which was a response to soil moisture stress to ameliorate the damaging effects of reactive oxygen species produced under stress conditions. Results also indicate that L. serriola can survive and produce seeds under water stress as more than 6000 seeds were produced per plant in all WHC treatments. In this study, there was no significant difference in the seed weight, number of seeds produced and their germination ability. This can have a huge impact on agricultural systems as the species can survive both under low and high soil moisture conditions. We therefore suggest that the demonstrated ability of L. serriola to complete its life cycle and produce biomass and seeds under water stressed conditions leads to the introduction of strategies that minimize weed survival while maximizing irrigation efficiency for the crop. A clear understanding of the ecological and biological characteristics of this weed will help land managers take appropriate control measures to mitigate the effect of this species on economic crop productivity.
Germination ecology of Chloris truncata and its implication for weed management
- Authors: Chauhan, Bhagirath , Manalil, Sudheesh , Florentine, Singarayer , Jha, Prashant
- Date: 2018
- Type: Text , Journal article
- Relation: PLoS ONE Vol. 13, no. 7 (2018), p. 1-13
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- Description: Chloris truncata is a significant weed in summer crops in the subtropical region of Australia. A study was conducted to evaluate the effect of environmental factors on germination and emergence of two populations of C. truncata. Overall, germination was not affected by the populations. Seeds germinated at a wide range of alternating day/night temperatures, suggesting that seeds can germinate throughout the spring, winter and autumn seasons. Seed germination was stimulated by the presence of light; however, 51 to 71% of these seeds still germinated in the dark. The sodium chloride concentration and osmotic potential required to inhibit germination of 50% of the population were 179 mM and -0.52 MPa, respectively. A high proportion of seeds germinated over a wide pH range (4 to 10). Seeds placed on the soil surface had greatest germination (67%) and a burial depth of 3 cm resulted in complete inhibition of emergence. The sorghum residue amount required to reduce emergence by 50% was 1.8 t ha-1. The results suggest that, although this weed will be favored in no-till systems, residue retention on the soil surface will help in reducing its infestation. Seed bank buildup can be managed by burying seeds below the depth of emergence. © 2018 Chauhan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The association of Social Anxiety Disorder, Alcohol Use Disorder and reproduction: Results from four nationally representative samples of adults in the USA
- Authors: Miloyan, Beyon , Bulley, Adam , Brilot, Ben , Suddendorf, Thomas
- Date: 2017
- Type: Text , Journal article
- Relation: PLoS ONE Vol. 12, no. 11 (2017), p.
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- Description: Social Anxiety Disorder (SAD) and Alcohol Use Disorder (AUD) are highly prevalent and frequently co-occur. The results of population studies suggest that SAD tends to precede AUD, and the results of laboratory studies suggest that alcohol use facilitates social behaviors in socially anxious individuals. Therefore, we posited that, in a modern context, a tendency to consume alcohol may be positively selected for among socially anxious individuals by its effect on the likelihood of finding a partner and reproducing. We tested the hypothesis that a higher proportion of individuals with a lifetime diagnosis of SAD and AUD reproduce (i.e., have at least one child) relative to individuals with SAD absent AUD in an individual participant meta-analysis based on over 65,000 adults derived from four nationally representative cross-sectional samples. We then cross-validated these findings against the results of a 10-year follow up of one of these surveys. Lifetime history of SAD was not associated with reproduction whereas lifetime history of AUD was positively associated with reproduction. There was no statistically detectable difference in the proportion of individuals with a lifetime history of SAD with or without AUD who reproduced. There was considerable heterogeneity in all of the analyses involving SAD, suggesting that there are likely to be other pertinent variables relating to SAD and reproduction that should be delineated.
Confirmatory factor analysis and exploratory structural equation modelling of the factor structure of the depression anxiety and stress scales-21
- Authors: Gomez, Rapson , Stavropoulos, Vasileios , Griffiths, Mark
- Date: 2020
- Type: Text , Journal article
- Relation: Plos One Vol. 15, no. 6 (Jun 2020), p. 16
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- Description: The Depression Anxiety and Stress Scales-21 (DASS-21) involves a simple structure first-order three-factor oblique model, with factors for depression, anxiety, and stress. Recently, concerns have been raised over the value of using confirmatory factor analysis (CFA) for studying the factor structure of scales in general. However, such concerns can be circumvented using exploratory structural equation modeling (ESEM). Consequently, the present study used CFA and ESEM with target rotation to examine the factor structure of the DASS-21 among an adult community. It compared first-order CFA, ESEM with target rotation, bi-factor CFA (BCFA), and bi-factor BESEM with target rotation models with group/specific factors for depression, anxiety, and stress. A total of 738 adults (males = 374, and females = 364; M = 25.29 years; SD = 7.61 years) completed the DASS-21. While all models examined showed good global fit values, one or more of the group/specific factors in the BCFA, ESEM with target rotation and BESEM with target rotation models were poorly defined. As the first-order CFA model was most parsimonious, with well-defined factors that were also supported in terms of their reliabilities and validities, this model was selected as the preferred DASS-21 model. The implications of the findings for use and revision of the DASS-21 are discussed.
Molecular phylogeny of Burkholderia pseudomallei from a remote region of Papua New Guinea
- Authors: Baker, Anthony , Pearson, Talima , Price, Erin , Dale, Julia , Keim, Paul , Hornstra, Heidie , Greenhill, Andrew , Padilla, Gabriel , Warner, Jeffrey
- Date: 2011
- Type: Text , Journal article
- Relation: PLoS ONE Vol. 6, no. 3 (2011), p.
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- Description: Background: The island of New Guinea is located midway between the world's two major melioidosis endemic regions of Australia and Southeast Asia. Previous studies in Papua New Guinea have demonstrated autochthonous melioidosis in Balimo, Western province. In contrast to other regions of endemicity, isolates recovered from both environmental and clinical sources demonstrate narrow genetic diversity over large spatial and temporal scales. Methodology/Principal Findings: We employed molecular typing techniques to determine the phylogenetic relationships of these isolates to each other and to others worldwide to aid in understanding the origins of the Papua New Guinean isolates. Multi-locus sequence typing of the 39 isolates resolved three unique sequence types. Phylogenetic reconstruction and Structure analysis determined that all isolates were genetically closer to those from Australia than those from Southeast Asia. Gene cluster analysis however, identified a Yersinia-like fimbrial gene cluster predominantly found among Burkholderia pseudomallei derived from Southeast Asia. Higher resolution VNTR typing and phylogenetic reconstruction of the Balimo isolates resolved 24 genotypes with long branch lengths. These findings are congruent with long term persistence in the region and a high level of environmental stability. Conclusions/Significance: Given that anthropogenic influence has been hypothesized as a mechanism for the dispersal of B. pseudomallei, these findings correlate with limited movement of the indigenous people in the region. The palaeogeographical and anthropogenic history of Australasia and the results from this study indicate that New Guinea is an important region for the further study of B. pseudomallei origins and dissemination.
Investigating cumulative effects of preperformance routine interventions in beach volleyball serving
- Authors: Wergin, Vanessa , Beckmann, Jurgen , Gröpel, Peter , Mesagno, Christopher
- Date: 2020
- Type: Text , Journal article
- Relation: PLoS ONE Vol. 15, no. 1 (2020), p.
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- Description: Pre-performance routines (PPRs) can be used in certain sports to minimize the effects of choking under pressure. This study aimed to investigate the cumulative effectiveness of PPR interventions on the accuracy of beach volleyball serves. Fifty-four beach volleyball players were randomly assigned to one of three PPR intervention groups or a control group. Participants performed 10 serves at a target on the opposite side of the beach volleyball court (pretest), were educated on a PPR intervention, and then completed 10 serves at the target under pressure that was induced through videotaping and ego-relevant instructions (posttest). The results indicated no difference in post-test serving accuracy among the intervention groups and the wait-list control group and no difference in effectiveness between cumulative and isolated PPR use. A possible explanation may be the inefficiency of the pressure manipulation. However, the null results related to isolated and cumulative PPR use under general (i.e., no pressure) conditions are still an important research finding. Future research should investigate the effectiveness of cumulative and other PPRs in other sports in general and under pressure. © 2020 Wergin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Lower dormancy with rapid germination is an important strategy for seeds in an arid zone with unpredictable rainfall
- Authors: Duncan, Corrine , Schultz, Nick , Lewandrowski, Wolfgang , Good, Megan , Cook, Simon
- Date: 2019
- Type: Text , Journal article
- Relation: PLoS ONE Vol. 14, no. 9 (2019), p.
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- Description: Seed germination traits are key drivers of population dynamics, yet they are under-represented in community ecology studies, which have predominately focussed on adult plant and seed morphological traits. We studied the seed traits and germination strategy of eight woody plant species to investigate regeneration strategies in the arid zone of eastern Australia. To cope with stochastic and minimal rainfall, we predict that arid seeds will either have rapid germination across a wide range of temperatures, improved germination under cooler temperatures, or dormancy and/or longevity traits to delay or stagger germination across time. To understand how temperature affects germination responses, seeds of eight keystone arid species were germinated under laboratory conditions, and under three diurnal temperatures (30/20°C, 25/15°C and 17/7°C) for 30 days. We also tested for decline in seed viability across 24 months in a dry-aging treatment (
Environmental factors effecting the germination and seedling emergence of two populations of an aggressive agricultural weed; Nassella trichotoma
- Authors: Humphries, Talia , Chauhan, Bhagirath , Florentine, Singarayer
- Date: 2018
- Type: Text , Journal article
- Relation: PLoS ONE Vol. 13, no. 7 (2018), p. 1-25
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- Description: Nassella trichotoma (Nees) Hack. ex Arechav. (Serrated tussock) is an aggressive globally significant weed to agricultural and natural ecosystems. Herbicide resistant populations of this C3 perennial weed have emerged, increasing the need for effective wide-scale cultural control strategies. A thorough seed ecology study on two spatially distinct populations of N. trichotoma was conducted on this weed to identify differences in important environmental factors (drought, salinity, alternating temperature, photoperiod, burial depth, soil pH, artificial seed aging, and radiant heat) which influence seed dormancy. Seeds were collected from two spatially distinct populations; Gnarwarre (38 O 9’ 8.892’’ S, 144 O 7’ 38.784’’ E) and Ingliston (37O 40’ 4.44’’ S, 144 O 18’ 39.24’’ E) in December 2016 and February 2017, respectively. Twenty sterilized seeds were placed into Petri dishes lined with a single Whatman® No. 10 filter paper dampened with the relevant treatments solution and then incubated under the identified optimal alternating temperature and photoperiod regime of 25C/ 15C (light/dark, 12h/12h). For the burial depth treatment, 20 seeds were placed into plastic containers (10cm in diameter and 6cm in depth) and buried to the relevant depth in sterilized soil. All trials were monitored for 30 days and germination was indicated by 5mm exposure of the radicle and emergence was indicated by the exposure of the cotyledon. Each treatment had three replicates for each population, and each treatment was repeated to give a total of six replicates per treatment, per population. Nassella trichotoma was identified to be non-photoblastic, with germination (%) being similar under alternating light and dark and complete darkness conditions. With an increase of osmotic potential and salinity, a significant decline in germination was observed. There was no effect of pH on germination. Exposure to a radiant heat of 120C for 9 minutes resulted in the lowest germination in the Ingliston population (33%) and the Gnarwarre population (60%). In the burial depth treatment, the Ingliston population and the Gnarwarre population had highest emergence of 75% and 80%, respectively at a depth of 1cm. Variation between the two populations was observed for the burial depth treatments; Gnarwarre had greater emergence than Ingliston from the 4cm burial depth, while Ingliston had greater emergence at the soil surface than Gnarwarre. The Gnarwarre population had greater overall germination than Ingliston, which could be attributed to the greater seed mass (0.86mg compared to 0.76mg, respectively). This study identifies that spatial variations in N. trichotoma’s seed ecology are present between spatially distinct populations.
Characterization of the gut microbiota of Papua New Guineans using reverse transcription quantitative PCR
- Authors: Greenhill, Andrew , Tsuji, Hirokazu , Ogata, Kiyohito , Natsuhara, Kazumi , Morita, Ayako , Soli, Kevin , Larkins, Jo-Ann , Tadokoro, Kiyoshi , Odani, Shingo , Baba, Jun , Naito, Yuichi , Tomitsuka, Eriko , Nomoto, Kriko , Siba, Peter , Horwood, Paul , Umezaki, Masahiro
- Date: 2015
- Type: Text , Journal article
- Relation: PLoS ONE Vol. 10, no. 2 (2015), p. 1-15
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- Description: There has been considerable interest in composition of gut microbiota in recent years, leading to a better understanding of the role the gut microbiota plays in health and disease. Most studies have been limited in their geographical and socioeconomic diversity to high-income settings, and have been conducted using small sample sizes. To date, few analyses have been conducted in low-income settings, where a better understanding of the gut microbiome could lead to the greatest return in terms of health benefits. Here, we have used quantitative real-time polymerase chain reaction targeting dominant and sub-dominant groups of microorganisms associated with human gut microbiome in 115 people living a subsistence lifestyle in rural areas of Papua New Guinea. Quantification of Clostridium coccoides group, C. leptum subgroup, C. perfringens, Bacteroides fragilis group, Bifidobacterium, Atopobium cluster, Prevotella, Enterobacteriaceae, Enterococcus, Staphylococcus, and Lactobacillus spp. was conducted. Principle coordinates analysis (PCoA) revealed two dimensions with Prevotella, clostridia, Atopobium, Enterobacteriaceae, Enterococcus and Staphylococcus grouping in one dimension, while B. fragilis, Bifidobacterium and Lactobacillus grouping in the second dimension. Highland people had higher numbers of most groups of bacteria detected, and this is likely a key factor for the differences revealed by PCoA between highland and lowland study participants. Age and sex were not major determinants in microbial population composition. The study demonstrates a gut microbial composition with some similarities to those observed in other low-income settings where traditional diets are consumed, which have previously been suggested to favor energy extraction from a carbohydrate rich diet. © 2015 PLOS ONE.
Belief in conspiracy theories : the predictive role of schizotypy, machiavellianism, and primary psychopathy
- Authors: March, Evita , Springer
- Date: 2019
- Type: Text , Journal article
- Relation: PLoS ONE Vol. 14, no. 12 (2019), p.
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- Description: A conspiracy theory refers to an alternative explanation of an event involving a conspirator plot organised by powerful people or organisations. Belief in conspiracy theories is related to negative societal outcomes such as poor medical decisions and a decrease in prosocial behaviour. Given these negative outcomes, researchers have explored predictors of belief in conspiracy theories in an attempt to understand and possibly manage these beliefs. In the current study, we explored the utility of personality in predicting belief in conspiracy theories. The aim of the current study was to explore the utility of the odd beliefs/magical thinking subtype of schizotypy, Machiavellianism, grandiose narcissism, vulnerable narcissism, primary psychopathy, and secondary psychopathy in predicting belief in conspiracy theories. Participants (N = 230; 44.7% male, 55.3% female) completed an anonymous, confidential online questionnaire which comprised demographics and measures of personality traits and belief in conspiracy theories. The total regression model indicated odd beliefs/magical thinking, trait Machiavellianism, and primary psychopathy were significant, positive predictors of belief in conspiracy theories. No other predictors reached significance. Results of the current study highlight individuals who might be more susceptible to believing conspiracy theories. Specifically, these results indicate that the individual more likely to believe in conspiracy theories may have unusual patterns of thinking and cognitions, be strategic and manipulative, and display interpersonal and affective deficits. © 2019 March, Springer. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A configural model of expert judgement as a preliminary epidemiological study of injury problems: An application to drowning
- Authors: Morgan, Damian , Ozanne-Smith, Joan
- Date: 2019
- Type: Text , Journal article
- Relation: PLoS ONE Vol. 14, no. 10 (2019), p.
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- Description: Robust epidemiological studies identifying determinants of negative health outcomes require significant research effort. Expert judgement is proposed as an efficient alternative or preliminary research design for risk factor identification associated with unintentional injury. This proposition was tested in a multi-factorial balanced experimental design using specialist judges (N = 18), lifeguards and surfers, to assess the risk contribution to drowning for swimming ability, surf bathing experience, and wave height. All factors provided unique contributions to drowning risk (p < .001). An interaction (p = .02) indicated that occasional surf bathers face a proportionally increased risk of drowning at increased wave heights relative to experienced surf bathers. Although findings were limited by strict criteria, and no gold standard comparison data were available, the study provides new evidence on causal risk factors for a drowning scenario. Countermeasures based on these factors are proposed. Further application of the method may assist in developing new interventions to reduce unintentional injury. © 2019 Morgan, Ozanne-Smith. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The effect of human amnion epithelial cells on lung development and inflammation in preterm lambs exposed to antenatal inflammation
- Authors: Papagianis, Paris , Ahmadi-Noorbakhsh, Siavash , Lim, Rebecca , Wallace, Euan , Polglase, Graeme , Pillow, J. Jane , Moss, Timothy
- Date: 2021
- Type: Text , Journal article
- Relation: PloS one Vol. 16, no. 6 (2021), p. e0253456-e0253456
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- Description: Lung inflammation and impaired alveolarization are hallmarks of bronchopulmonary dysplasia (BPD). We hypothesize that human amnion epithelial cells (hAECs) are anti-inflammatory and reduce lung injury in preterm lambs born after antenatal exposure to inflammation. Pregnant ewes received either intra-amniotic lipopolysaccharide (LPS, from E.coli 055:B5 4mg) or saline (Sal) on day 126 of gestation. Lambs were delivered by cesarean section at 128 d gestation (term ~150 d). Lambs received intravenous hAECs (LPS/hAECs: n = 7 30x10.sup.6 cells) or equivalent volumes of saline (LPS/Sal, n = 10 or Sal/Sal, n = 9) immediately after birth. Respiratory support was gradually de-escalated, aimed at early weaning from mechanical ventilation towards unassisted respiration. Lung tissue was collected 1 week after birth. Lung morphology was assessed and mRNA levels for inflammatory mediators were measured. Respiratory support required by LPS/hAEC lambs was not different to Sal/Sal or LPS/Sal lambs. Lung tissue:airspace ratio was lower in the LPS/Sal compared to Sal/Sal lambs (P<0.05), but not LPS/hAEC lambs. LPS/hAEC lambs tended to have increased septation in their lungs versus LPS/Sal (P = 0.08). Expression of inflammatory cytokines was highest in LPS/hAECs lambs. Postnatal administration of a single dose of hAECs stimulates a pulmonary immune response without changing ventilator requirements in preterm lambs born after intrauterine inflammation.