Overcoming liver fluke as a constraint to ruminant production in South-East Asia
- Authors: Piedrafita, David , Estuningsih, Endah , Spithill, Terry , Pleasance, Jill , Widjajanti, Sri , Suharyanta, S. , Partoutomo, Sutijono , Kingsford, Nicola , Hansen, D. , Clery, Dianne , Prowse, Rhoda , Meeusen, Els , Raadsma, Herman
- Date: 2008
- Type: Text , Book
- Full Text: false
- Reviewed:
Innate and adaptive resistance of Indonesian Thin Tail sheep to liver fluke: A comparative analysis of Fasciola gigantica and Fasciola hepatica infection
- Authors: Pleasance, Jill , Raadsma, Herman , Estuningsih, S. , Widjajanti, Sri , Meeusen, Els , Piedrafita, David
- Date: 2011
- Type: Text , Journal article
- Relation: Veterinary Parasitology Vol. 178, no. 3-4 (2011), p. 264-272
- Full Text: false
- Reviewed:
- Description: In the current study, three independent trials directly compared Fasciola gigantica and Fasciola hepatica infection of ITT sheep. In all trials, F. hepatica infection resulted in higher worm burden recoveries and greater physiological damage to ITT sheep. Developmental differences of the two Fasciola species were also observed during the first twelve weeks of a primary infection, where the migration and growth of F. hepatica was more rapid than F. gigantica. Various immunological blood parameters were measured and indicated similar kinetics in the humoral and cellular responses during the time course of infection with each Fasciola species. In contrast to F. hepatica infection, we demonstrate an innate and adaptive comparative ability of ITT sheep to resist the early stages of infection with F. gigantica infection. Unraveling the mechanisms leading to this differential resistance may potentially lead to new methods for the control of fasciolosis and other human liver flukes.
The kinetics of local cytokine and galectin expression after challenge infection with the gastrointestinal nematode, Haemonchus contortus
- Authors: Robinson, Nicholas , Pleasance, Jill , Piedrafita, David , Meeusen, Els
- Date: 2011
- Type: Text , Journal article
- Relation: International Journal for Parasitology Vol. 41, no. 5 (2011 2011), p. 487-493
- Full Text: false
- Reviewed:
- Description: Gastrointestinal nematode parasites undergo several developmental stages within their mammalian host, each presenting different antigenic challenges to the immune system. To examine the expression of different immune mediators over time, biopsy samples were collected from the cannulated abomasum (true stomach) of immune sheep at several times after a challenge infection with Haemonchus contortus L3s. IL-5 and IL-13 mRNA expression levels were significantly increased above saline-challenged control levels at 5 and 7 days post challenge, while IL-4 showed an earlier peak at day 2 post challenge. IL-5, IL-13 and IL-4, as well as IFN-
Resistance to liver fluke infection in the natural sheep host is correlated with a type-1 cytokine response
- Authors: Pleasance, Jill , Wiedosari, E. , Raadsma, Herman , Meeusen, Els , Piedrafita, David
- Date: 2011
- Type: Text , Journal article
- Relation: Parasite Immunology Vol. 33, no. 9 (2011), p. 495-505
- Full Text: false
- Reviewed:
- Description: Indonesian thin-tail (ITT) sheep can resist infection with Fasciola gigantica but not F. hepatica and presents an ideal model to investigate the mechanisms of liver fluke resistance in a natural host. This study examines the local and systemic immune responses of sheep during Fasciola infection and demonstrates that different anatomical tissues display distinct cytokine profiles consistent with liver fluke migration. The study also reveals a significant difference in the cytokine and antibody profiles of ITT sheep infected with F. gigantica compared with F. hepatica, with a higher ratio of IL-4/IFN-