Application of high-speed countercurrent chromatography for the isolation of sulforaphane from broccoli seed meal
- Authors: Liang, Hao , Li, Chunfang , Yuan, Qipeng , Vriesekoop, Frank
- Date: 2008
- Type: Text , Journal article
- Relation: Journal of Agricultural and Food Chemistry Vol. 56, no. 17 (2008), p. 7746-7749
- Full Text: false
- Description: In order to produce large amounts of pure sulforaphane for research purposes, a novel method using high-speed countercurrent chromatography (HSCCC) was developed. Without any initial cleanup steps, sultoraphane was successfully purified from the ethyl acetate extract of the broccoli seed meal by HSCCC. The separation was performed with two-phase solvent systems: n-hexane/ethyl acetate/ methanol/water (1:5:1:5, v/v/v/v). From 850 mg of the ethyl acetate extract, 186 mg of sultoraphane was isolated with the solvent system. The purified compound was over 97% purity as determined by HPLC analysis, and the chemical structure was confirmed by MS and 1H and 13C NMR. © 2008 American Chemical Society.
Separation and purification of sulforaphane from broccoli seeds by solid phase extraction and preparative high-performance liquid chromatography
- Authors: Liang, Hao , Li, Chunfang , Yuan, Qipeng , Vriesekoop, Frank
- Date: 2007
- Type: Text , Journal article
- Relation: Journal of Agricultural and Food Chemistry Vol. 55, no. 20 (2007), p. 8047-8053
- Full Text: false
- Reviewed:
- Description: A novel, rapid, and economical method to isolate and purify natural sulforaphane from broccoli seeds is described. The procedure involves solvent extraction of autolyzed seed meal, followed by separation by solid phase extraction (SPE) and purification by preparative high-performance liquid chromatography (HPLC). The SPE method provides higher yield of sulforaphane from crude extracts compared to conventional liquid-liquid extraction. High purity and recovery of sulforaphane product can be obtained by preparative HPLC with a C18 column and 30% methanol in water as the mobile phase. The purified compound was characterized by MS and 1H and 13C NMR. The techniques described here are useful tools in the preparative-scale isolation of sulforaphane in a fast, cost-effective, and waste-conscious manner. © 2007 American Chemical Society.
- Description: C1
- Description: 2003004835