Survival and fermentation activity of probiotic bacteria and oxidative stability of omega-3 oil in co-microcapsules during storage
- Authors: Eratte, Divya , Wang, Bo , Dowling, Kim , Barrow, Colin , Adhikari, Benu
- Date: 2016
- Type: Text , Journal article
- Relation: Journal of Functional Foods Vol. 23, no. (2016), p. 485-496
- Full Text: false
- Reviewed:
- Description: Tuna oil (O) and probiotic bacteria Lactobacillus casei (P) were co-microencapsulated in whey protein isolate (WPI)-gum Arabic (GA) complex coacervate. The co-microcapsules (WPI-P-O-GA), L. casei microcapsules (WPI-P-GA) and tuna oil microcapsules (WPI-O-GA) were converted into powder using spray and freeze drying. The interaction between probiotic bacteria and omega-3 oil in co-microcapsules, particularly in terms of oxidative stability of omega-3 oil and vitality/viability of probiotic bacteria and any synergistic outcome, was studied. The effect of storage temperature (5 and 25 °C) and time (90 days) on the survival and fermentation activity of L. casei and oxidative stability of tuna oil in the microcapsules/co-microcapsules was determined. A synergism between oxidative stability of omega-3 oil and vitality of probiotic bacteria was observed, when they were co-microencapsulated and spray dried. These co-microcapsules will likely have utility in functional food formulations due to simple and cost effective stabilisation and delivery of two important functional ingredients. © 2016 Elsevier Ltd.
Co-encapsulation of Omega-3 fatty acids and probiotic bacteria through complex coacervation
- Authors: Eratte, Divya
- Date: 2016
- Type: Text , Thesis , PhD
- Full Text:
- Description: The research described in this thesis investigated the microencapsulation of omega-3 oil and probiotic bacteria together in a protein-polysaccharide complex coacervate matrix. The synergistic or competitive interactions between the probiotic bacteria and omega-3 fatty acids when packaged in a single microcapsule was determined including how best to utilise such interaction to achieve improved oxidative stability of omega-3 fatty acid and better survival of the probiotic bacteria. Encapsulation and co-encapsulation of tuna oil (O) and Lactobacillus casei 431 (P) as models of omega-3 and probiotic bacteria, respectively, were carried out and the works is described in this thesis in five distinct sections. (1) The optimisation of the complex coacervation process between whey protein isolate (WPI) and gum Arabic (GA). (2) Microencapsulation of tuna oil (O) in WPI-GA complex coacervates followed by spray and freeze drying to produce microcapsules (WPI-O-GA). (3) Microencapsulation of probiotic bacteria L. casei 431 (P) in WPI-GA complex coacervates followed by spray and freeze drying to produce microcapsules (WPI-P-GA). (4) Co-encapsulation of omega-3 oil and L. casei 431 together in WPI-GA coacervate matrix followed by spray and freeze drying to produce co-microcapsules (WPI-P-O-GA). (5) In-vitro digestion evaluation of co-microcapsules and microcapsules to indicate bioavailability. The viability of L. casei was significantly higher in WPI-P-O-GA co-microcapsules than in WPI-P-GA microcapsules in both spray and freeze dried microcapsules. The oxidative stability of tuna oil was significantly higher in spray dried co-capsules. Also, co-microencapsulation increased the survivability of L. casei during simulated digestion. There was no significant influence observed on the release properties of omega-3 oil due to co-microencapsulation. However, the total omega-3 fatty acids in the released oil during in-vitro digestion were found to be higher, when co-microencapsulated. Hence, co-microencapsulation was shown to protect the L. casei and deliver both viable cells and omega-3 oil to human intestine without any significant adverse effect on their functionality and properties.
- Description: Doctor of Philosophy
Co-encapsulation and characterisation of omega-3 fatty acids and probiotic bacteria in whey protein isolate-gum Arabic complex coacervates
- Authors: Eratte, Divya , McKnight, Stafford , Gengenbach, Thomas , Dowling, Kim , Barrow, Colin , Adhikari, Benu
- Date: 2015
- Type: Text , Journal article
- Relation: Journal of Functional Foods Vol. 19, no. (2015), p. 882-892
- Full Text: false
- Reviewed:
- Description: Omega-3 fatty acids and probiotic bacteria were co-encapsulated in a single whey protein isolate (WPI)-gum Arabic (GA) complex coacervate microcapsule. Tuna oil (0) and Lactobacillus casei 431 (P) were used as models of omega-3 and probiotic bacteria, respectively. The co-microcapsules (WPI-P-O-GA) and L. casei containing microcapsules (WPI-P-GA) were converted into powder by using spray and freeze drying. The viability of L. casei was significantly higher in WPI-P-O-GA co-microcapsules than in WPI-P-GA. The oxidative stability of tuna oil was significantly higher in spray dried co-capsules than in freeze dried ones. Crown Copyright (C) 2015 Published by Elsevier Ltd. All rights reserved.