- Title
- Microencapsulation of omega-3 fatty acids from flaxseed oil in flaxseed protein and flaxseed gum based matrix
- Creator
- Kaushik, Pratibha
- Date
- 2016
- Type
- Text; Thesis; PhD
- Identifier
- http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/154235
- Identifier
- vital:11066
- Identifier
- http://library.federation.edu.au/record=b2684898
- Abstract
- The overarching goal of this research was to develop microencapsulated flaxseed oil as a plant based source of omega-3 fatty acids. To accomplish this, flaxseed oil was microencapsulated in a novel matrix composed of flaxseed protein isolate (FPI) and flaxseed gum (FG) and converted into a powder by freeze drying and spray drying. The primary objectives were: a) To evaluate the physicochemical and functional properties of FPI and FG; b) to optimise the process of complex coacervation between FPI and FG to maximise the yield of complex coacervates; c) to characterise the flaxseed oil microcapsules obtained through complex coacervation followed by freeze drying or spray drying. FPI and FG were extracted from whole flaxseeds at optimised temperatures to obtain 90% purity. The physicochemical and functional properties of FPI were found superior to most of the commonly used proteins. Lower extraction temperatures (30, 50 °C) of FG yielded higher levels of neutral monosaccharides and lower levels of acidic monosaccharides. The functional properties of FG, such as EAI and WAC, were negatively affected by the rise in extraction temperature. Electrostatic complexation studies between these two biopolymers showed that the optimum FPI-to-FG ratio is 3:1 and the optimum pH is 3.1. The complex coacervates of FPI-FG were used to microencapsulate flaxseed oil at different core to wall ratios (1:2, 1:3 and 1:4), and converted to powder through spray drying and freeze drying. The spray dried solid microcapsules had higher oil microencapsulation efficiency, lower surface oil content and higher oxidation stability compared to the freeze dried microcapsules. The oxidation stability obtained from spray dried microcapsules at core-to-wall ratio of 1:4 was nearly double to that of the unencapsulated flaxseed oil. This study affirms the potential of a solely plant based encapsulating matrix that returns superior nutritional outcomes to other commonly used wall materials.; Doctor of Philosophy
- Publisher
- Federation University Australia
- Rights
- Copyright Pratibha Kaushik
- Rights
- Open Access
- Rights
- This metadata is freely available under a CCO license
- Subject
- Microencapsulation; Omega-3 fatty acids; Flaxseed
- Full Text
- Thesis Supervisor
- Dowling, Kim
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