The penetration of methanol into bovine cardiac and hepatic tissues is faster than ethanol and formalin
- Authors: Steicke, Michelle , Yang, Guang , Dinh, Tam , Dunster-Jones, Matthew , Sargisson, Owen , Ahmady, Farah , Golledge, Jonathan , Wang, Yutang
- Date: 2018
- Type: Text , Journal article
- Relation: European Journal of Histochemistry Vol. 62, no. 1 (2018), p. 98-104
- Relation: http://purl.org/au-research/grants/nhmrc/1062671
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- Description: Methanol, ethanol and formalin are commonly used as fixatives to preserve biological tissues from decay in the preparation of histological sections. Fixation of the inner layers of the tissue depends on the ability of the fixative to diffuse into the tissue. It is unknown whether methanol penetrates tissues at similar rates to other fixatives. This study aimed to compare the penetration rates of methanol, ethanol and formalin into bovine heart and liver tissues. The penetration distance and tissue shrinkage or expansion were measured by analysing the digital images of tissue before and after immersion in different fixatives for 1, 2, 6 or 10 h. Data were analysed using two-way ANOVA, followed by Bonferroni’s post-hoc test. The penetration distance of methanol was significantly greater in both heart and liver tissues compared with that of ethanol (N=4, P<0.001). Methanol or ethanol immersion led to similar shrinkage of both tissues (P>0.05). The penetration rate of formalin was similar to that of ethanol in both tissues however it was significantly slower than methanol (N=4, P<0.005 in the heart; P<0.001 in the liver). The mean penetration coefficients of methanol, formalin and ethanol in the heart tissue were 2.609, 1.994 and 1.801, respectively, and 3.012, 2.153 and 2.113, respectively, in the liver tissue. The penetration coefficient of methanol was significantly greater than that of ethanol or formalin in both tissues (P<0.001 for each comparison). In conclusion, methanol penetrates tissue significantly faster than ethanol and formalin.
A review on chemical diagnosis techniques for transformer paper insulation degradation
- Authors: Abu Bakar, Norazhar , Abu Siada, Ahmed , Islam, Syed
- Date: 2013
- Type: Text , Conference proceedings , Conference paper
- Relation: 2013 Australasian Universities Power Engineering Conference, AUPEC 2013; Hobart, Australia; 29th September-3rd October 2013 p. 1-6
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- Description: Energized parts within power transformer are isolated using paper insulation and are immersed in insulating oil. Hence, transformer oil and paper insulation are essential sources to detect incipient and fast developing power transformer faults. Several chemical diagnoses techniques are developed to examine the condition of paper insulation such as degree of polymerization, carbon oxides, furanic compounds and methanol. The principle and limitation of these diagnoses are discussed and compared in this paper.