Gas chromatographic-mass spectrometry analysis of volatile organic compounds from cancer cell cultures - The effect of hypoxia
- Authors: Kalluri, Usha
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Early diagnosis of lung cancer improves patient outcomes which has led to a search for non-invasive diagnostic tests suitable for population screening. Volatile organic compounds (VOCs) in exhaled breath have shown potential, however, confirmation of the metabolic origins and disease specificity of candidate markers is required. Cell culture metabolomics can identify disease biomarkers and their origins. To date VOC profiles from in vitro cultured cancer cells have little similarity to cancer breath VOC profiles. In vivo, cancer cells experience hypoxia whereas in vitro cells are cultured under normoxic conditions. Since hypoxia influences cell metabolism, we hypothesize that cancer cells cultured under hypoxic conditions will have altered cell metabolism and produce VOC profiles more typical of cancer breathe. This study investigates the effect of hypoxia on metabolic reprogramming in A549 lung cancer cells cultured under standard normoxic (atmospheric oxygen) or hypoxic (2% oxygen) conditions. Results from quantitative RT-PCR demonstrated a significant upregulation in hypoxia of the glucose transporter (GLUT1) and the key TCA regulatory gene PDHK1, demonstrating that hypoxia plays a pivotal role in regulating metabolism in A549 cells. A ratio-metric assessment of Lipid Peroxidation (LPO) and the production of reactive oxygen species (ROS) showed an increase in LPO and a slight decrease in the production of ROS in hypoxic cultures, the combined effect of which may serve to equip the cells to adapt to and proliferate under low oxygen. Finally, the comparison of endogenous VOCs produced by A549 cells under hypoxic and normoxic conditions identified twelve VOCs unique to cells grown under hypoxic conditions including n-pentane, a marker of LPO and cancer, and 3-methyl hexane, which has been reported as a biomarker of cancer. This data is consistent with the hypothesis that a hypoxic tumour microenvironment may influence cell metabolism leading to a unique and diagnostic cancer VOC profile.
- Description: Doctor of Philosophy
- Authors: Kalluri, Usha
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Early diagnosis of lung cancer improves patient outcomes which has led to a search for non-invasive diagnostic tests suitable for population screening. Volatile organic compounds (VOCs) in exhaled breath have shown potential, however, confirmation of the metabolic origins and disease specificity of candidate markers is required. Cell culture metabolomics can identify disease biomarkers and their origins. To date VOC profiles from in vitro cultured cancer cells have little similarity to cancer breath VOC profiles. In vivo, cancer cells experience hypoxia whereas in vitro cells are cultured under normoxic conditions. Since hypoxia influences cell metabolism, we hypothesize that cancer cells cultured under hypoxic conditions will have altered cell metabolism and produce VOC profiles more typical of cancer breathe. This study investigates the effect of hypoxia on metabolic reprogramming in A549 lung cancer cells cultured under standard normoxic (atmospheric oxygen) or hypoxic (2% oxygen) conditions. Results from quantitative RT-PCR demonstrated a significant upregulation in hypoxia of the glucose transporter (GLUT1) and the key TCA regulatory gene PDHK1, demonstrating that hypoxia plays a pivotal role in regulating metabolism in A549 cells. A ratio-metric assessment of Lipid Peroxidation (LPO) and the production of reactive oxygen species (ROS) showed an increase in LPO and a slight decrease in the production of ROS in hypoxic cultures, the combined effect of which may serve to equip the cells to adapt to and proliferate under low oxygen. Finally, the comparison of endogenous VOCs produced by A549 cells under hypoxic and normoxic conditions identified twelve VOCs unique to cells grown under hypoxic conditions including n-pentane, a marker of LPO and cancer, and 3-methyl hexane, which has been reported as a biomarker of cancer. This data is consistent with the hypothesis that a hypoxic tumour microenvironment may influence cell metabolism leading to a unique and diagnostic cancer VOC profile.
- Description: Doctor of Philosophy
Green bonds : Do they bring sustainability to environmentally friendly projects?
- Authors: Nanayakkara, Madurika
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: This study was motivated by the urgent requirement to scale up the global green bond (GB) market to meet the trillion-dollar financial requirement of environmental resilience projects worldwide, and by the absence of prior scientific investigations to determine solutions to the GB market’s problems from different perspectives. Three empirical studies were conducted to provide a holistic view of the GB market. The first study investigated the perception gaps of different stakeholders in the GB market in terms of assessing the ‘greenness’ of projects funded through GBs, investors’ expectations of GB investments, and the factors hindering the growth of the GB market. A qualitative method was employed with document review, followed by interviews, a questionnaire survey and peer debriefing. The study found that both the demand and supply sides of the GB market are inadequate to meet the world’s green investment requirements. The main obstacles hindering the market growth are fear for greenwashing, definitions that lack clarity, the absence of large-scale issues, and the high costs associated with complex reporting processes and third-party certifications. The second study assessed the influence of GB principles on investor demand for GBs, using global GBs issued for the period 2007 to 2016. Bid–ask spread and yield spread were used to measure the investor demand. The results indicated a significant positive relationship between the degree of compliance and investor demand. The third study compared the credit spreads of corporate GBs and conventional bonds, measured by option-adjusted spread daily data for the period 2016 to 2017 worldwide. Hybrid method of panel data regression was employed to analyse the data, and found that GBs are traded at a premium in the world market, compared with conventional bonds. This thesis suggests several policy implications based on the findings of these studies to scale up the GB market as a new source of financing.
- Description: Doctor of Philosophy
- Authors: Nanayakkara, Madurika
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: This study was motivated by the urgent requirement to scale up the global green bond (GB) market to meet the trillion-dollar financial requirement of environmental resilience projects worldwide, and by the absence of prior scientific investigations to determine solutions to the GB market’s problems from different perspectives. Three empirical studies were conducted to provide a holistic view of the GB market. The first study investigated the perception gaps of different stakeholders in the GB market in terms of assessing the ‘greenness’ of projects funded through GBs, investors’ expectations of GB investments, and the factors hindering the growth of the GB market. A qualitative method was employed with document review, followed by interviews, a questionnaire survey and peer debriefing. The study found that both the demand and supply sides of the GB market are inadequate to meet the world’s green investment requirements. The main obstacles hindering the market growth are fear for greenwashing, definitions that lack clarity, the absence of large-scale issues, and the high costs associated with complex reporting processes and third-party certifications. The second study assessed the influence of GB principles on investor demand for GBs, using global GBs issued for the period 2007 to 2016. Bid–ask spread and yield spread were used to measure the investor demand. The results indicated a significant positive relationship between the degree of compliance and investor demand. The third study compared the credit spreads of corporate GBs and conventional bonds, measured by option-adjusted spread daily data for the period 2016 to 2017 worldwide. Hybrid method of panel data regression was employed to analyse the data, and found that GBs are traded at a premium in the world market, compared with conventional bonds. This thesis suggests several policy implications based on the findings of these studies to scale up the GB market as a new source of financing.
- Description: Doctor of Philosophy
Investigating bird responses to fire in the Heathy Dry Forests of Victoria, Australia
- Authors: Kuchinke, Diana
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Globally, forest birds are under pressure, from agriculture and urban development fragmenting the landscape. Adding to these pressures, changes in the patterns of global climate drivers give rise to an increase in the frequency of extreme weather events. In Victoria, Australia, changing weather conditions are resulting in increases in the frequency and extent of bushfires. Furthermore, prescribed burning is applied to the landscape in attempts to not only ameliorate the impacts from bushfire, but as part of a ‘pyrodiversity begets biodiversity’ protocol. These pressures all impact birds by reducing resources for: food, nesting and protection from predators. This thesis investigated bird responses to fire in the Heathy Dry Forests of Victoria, against variables of time-since-fire and fire frequency. Bird responses were modelled in terms of: community, foraging guilds and individual species. As a community, birds showed a resilience to both time since fire and fire frequency. Responses by foraging guilds and individual species highlighted some different responses. The common species from each foraging guild showed responses that broadly represent their guild. This thesis highlighted that an effective tool in adaptive management is to predict the trends of our common forest birds, as surrogates for entire bird communities, not just for fire responses, but for a broader reflection on the health of the landscape. The modelling of one species, the Laughing Kookaburra, showed a response to both time since fire and fire frequency, with a reduced abundance in post-fire new-growth vegetation. As this species is noted as being in decline down the east coast of Australia, it is flagged in this thesis as a species of concern. Further, this thesis investigated alpha and beta responses by the forest birds to prescribed burns of different severities. Results suggest that forest birds show little response to small prescribed burns in the landscape, regardless of severity. This may be a result of Heathy Dry Forests’ rapid regeneration post-fire. However, one species that exhibits site fidelity, the White-throated Treecreeper, left areas impacted by high severity prescribed burns. The White-throated Treecreeper’s response flagged the importance of tree hollows being maintained in the landscape, essential for the species that require hollows for roosting and nesting.
- Description: Doctor of Philosophy
- Description: Globally, forest birds are under pressure, from agriculture and urban development fragmenting the landscape. Adding to these pressures, changes in the patterns of global climate drivers give rise to an increase in the frequency of extreme weather events. In Victoria, Australia, changing weather conditions are resulting in increases in the frequency and extent of bushfires. Furthermore, prescribed burning is applied to the landscape in attempts to not only ameliorate the impacts from bushfire, but as part of a ‘pyrodiversity begets biodiversity’ protocol. These pressures all impact birds by reducing resources for: food, nesting and protection from predators. This thesis investigated bird responses to fire in the Heathy Dry Forests of Victoria, against variables of time-since-fire and fire frequency. Bird responses were modelled in terms of: community, foraging guilds and individual species. As a community, birds showed a resilience to both time since fire and fire frequency. Responses by foraging guilds and individual species highlighted some different responses. The common species from each foraging guild showed responses that broadly represent their guild. This thesis highlighted that an effective tool in adaptive management is to predict the trends of our common forest birds, as surrogates for entire bird communities, not just for fire responses, but for a broader reflection on the health of the landscape. The modelling of one species, the Laughing Kookaburra, showed a response to both time since fire and fire frequency, with a reduced abundance in post-fire new-growth vegetation. As this species is noted as being in decline down the east coast of Australia, it is flagged in this thesis as a species of concern. Further, this thesis investigated alpha and beta responses by the forest birds to prescribed burns of different severities. Results suggest that forest birds show little response to small rescribed burns in the landscape, regardless of severity. This may be a result of Heathy Dry Forests’ rapid regeneration post-fire. However, one species that exhibits site fidelity, the White-throated Treecreeper, left areas impacted by high severity prescribed burns. The White-throated Treecreeper’s response flagged the importance of tree hollows being maintained in the landscape, essential for the species that require hollows for roosting and nesting.
- Authors: Kuchinke, Diana
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Globally, forest birds are under pressure, from agriculture and urban development fragmenting the landscape. Adding to these pressures, changes in the patterns of global climate drivers give rise to an increase in the frequency of extreme weather events. In Victoria, Australia, changing weather conditions are resulting in increases in the frequency and extent of bushfires. Furthermore, prescribed burning is applied to the landscape in attempts to not only ameliorate the impacts from bushfire, but as part of a ‘pyrodiversity begets biodiversity’ protocol. These pressures all impact birds by reducing resources for: food, nesting and protection from predators. This thesis investigated bird responses to fire in the Heathy Dry Forests of Victoria, against variables of time-since-fire and fire frequency. Bird responses were modelled in terms of: community, foraging guilds and individual species. As a community, birds showed a resilience to both time since fire and fire frequency. Responses by foraging guilds and individual species highlighted some different responses. The common species from each foraging guild showed responses that broadly represent their guild. This thesis highlighted that an effective tool in adaptive management is to predict the trends of our common forest birds, as surrogates for entire bird communities, not just for fire responses, but for a broader reflection on the health of the landscape. The modelling of one species, the Laughing Kookaburra, showed a response to both time since fire and fire frequency, with a reduced abundance in post-fire new-growth vegetation. As this species is noted as being in decline down the east coast of Australia, it is flagged in this thesis as a species of concern. Further, this thesis investigated alpha and beta responses by the forest birds to prescribed burns of different severities. Results suggest that forest birds show little response to small prescribed burns in the landscape, regardless of severity. This may be a result of Heathy Dry Forests’ rapid regeneration post-fire. However, one species that exhibits site fidelity, the White-throated Treecreeper, left areas impacted by high severity prescribed burns. The White-throated Treecreeper’s response flagged the importance of tree hollows being maintained in the landscape, essential for the species that require hollows for roosting and nesting.
- Description: Doctor of Philosophy
- Description: Globally, forest birds are under pressure, from agriculture and urban development fragmenting the landscape. Adding to these pressures, changes in the patterns of global climate drivers give rise to an increase in the frequency of extreme weather events. In Victoria, Australia, changing weather conditions are resulting in increases in the frequency and extent of bushfires. Furthermore, prescribed burning is applied to the landscape in attempts to not only ameliorate the impacts from bushfire, but as part of a ‘pyrodiversity begets biodiversity’ protocol. These pressures all impact birds by reducing resources for: food, nesting and protection from predators. This thesis investigated bird responses to fire in the Heathy Dry Forests of Victoria, against variables of time-since-fire and fire frequency. Bird responses were modelled in terms of: community, foraging guilds and individual species. As a community, birds showed a resilience to both time since fire and fire frequency. Responses by foraging guilds and individual species highlighted some different responses. The common species from each foraging guild showed responses that broadly represent their guild. This thesis highlighted that an effective tool in adaptive management is to predict the trends of our common forest birds, as surrogates for entire bird communities, not just for fire responses, but for a broader reflection on the health of the landscape. The modelling of one species, the Laughing Kookaburra, showed a response to both time since fire and fire frequency, with a reduced abundance in post-fire new-growth vegetation. As this species is noted as being in decline down the east coast of Australia, it is flagged in this thesis as a species of concern. Further, this thesis investigated alpha and beta responses by the forest birds to prescribed burns of different severities. Results suggest that forest birds show little response to small rescribed burns in the landscape, regardless of severity. This may be a result of Heathy Dry Forests’ rapid regeneration post-fire. However, one species that exhibits site fidelity, the White-throated Treecreeper, left areas impacted by high severity prescribed burns. The White-throated Treecreeper’s response flagged the importance of tree hollows being maintained in the landscape, essential for the species that require hollows for roosting and nesting.
Investigating the impact of participation in alternative education from a positive youth development perspective : A case study of the School for Student Leadership
- Authors: Joyce, Susan
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: The School for Student Leadership (SSL) has existed since 2001 as an alternative residential educational setting for Year 9 students in Victoria, Australia. It was designed in response to growing calls for more effective ways of engaging middle school students. A review of the literature illustrated a dearth of research regarding alternative programs and schools, particularly from a positive youth development (PYD) perspective, and relating to long term effects of participation. This study investigated student participants’ perceived development in relation to the Five Cs - Confidence, Competence, Character, Connection and Care, concepts derived from the framework of PYD. It also examined differences in perceptions of students attending a shorter five-week program, compared to the standard nine-week program, as well as differences between male and female participants. This case study was undertaken utilising a mixed methods approach, using pre and post program surveys and interviews. Between 2013 and 2014, 385 students were surveyed and 58 interviews conducted over nine program offerings. The participants were then followed up one year post program, with 172 surveys returned and 14 interviews conducted. With the assistance of computer software programs, both the quantitative and qualitative data was analysed and then triangulated to form the findings. Participants’ ratings for each of the Five Cs were higher at the end of the program, and effect sizes, though generally small, when combined with the qualitative data indicated the program as having a significant positive impact, although this diminished slightly one year post program. Little discernible difference was found between the five- and the nine-week programs, and only slight differences between the two genders. The set of recommendations that emanated from this research should be of assistance for both the SSL and other schools, particularly in relation to enhancing attributes such as the Five Cs.
- Description: Doctor of Philosophy
- Authors: Joyce, Susan
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: The School for Student Leadership (SSL) has existed since 2001 as an alternative residential educational setting for Year 9 students in Victoria, Australia. It was designed in response to growing calls for more effective ways of engaging middle school students. A review of the literature illustrated a dearth of research regarding alternative programs and schools, particularly from a positive youth development (PYD) perspective, and relating to long term effects of participation. This study investigated student participants’ perceived development in relation to the Five Cs - Confidence, Competence, Character, Connection and Care, concepts derived from the framework of PYD. It also examined differences in perceptions of students attending a shorter five-week program, compared to the standard nine-week program, as well as differences between male and female participants. This case study was undertaken utilising a mixed methods approach, using pre and post program surveys and interviews. Between 2013 and 2014, 385 students were surveyed and 58 interviews conducted over nine program offerings. The participants were then followed up one year post program, with 172 surveys returned and 14 interviews conducted. With the assistance of computer software programs, both the quantitative and qualitative data was analysed and then triangulated to form the findings. Participants’ ratings for each of the Five Cs were higher at the end of the program, and effect sizes, though generally small, when combined with the qualitative data indicated the program as having a significant positive impact, although this diminished slightly one year post program. Little discernible difference was found between the five- and the nine-week programs, and only slight differences between the two genders. The set of recommendations that emanated from this research should be of assistance for both the SSL and other schools, particularly in relation to enhancing attributes such as the Five Cs.
- Description: Doctor of Philosophy
Lime stabilisation of an Australian silty clay and its application in construction of excavation retaining walls by cutter soil mixing
- Authors: Ranjbar Pouya, Kaveh
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Coode Island Silt (CIS) is one of the predominant geological units in Melbourne, Australia. Having high compressibility and low shear strength, CIS is considered a problematic soft soil that challenges the construction of infrastructure in the region. To tackle such challenges, one practical approach is the application of ground improvement techniques such as in situ soil mixing. This PhD study focuses on the application of Cutter Soil Mixing (CSM) for the construction of excavation retaining walls in CIS. Although cement is widely used in most CSM projects, this study investigates the suitability of different lime types available in the Australian market as a potential alternative to cement for the stabilisation of CIS. To investigate the effect of lime stabilisation, a comprehensive geotechnical characterisation of untreated and lime treated CIS is performed. Four different lime types are used: agricultural lime, quicklime, hydrated lime and slag lime. Based on the results obtained from strength tests, slag lime was found to be the most effective among the four types that were tested. The optimum slag lime to CIS ratio is then found for the construction of retaining walls in CIS. Having the geotechnical characterisation of untreated and treated CIS from the laboratory experiments, a series of two-dimensional and three-dimensional finite element method (FEM) analyses were conducted to investigate the applicability and reliability of the selected mixing ratio for the construction of CSM excavation retaining walls in CIS. A nonlinear constitutive soil model was employed, calibrated and verified to be used in FEM analyses to investigate both the stability factor of safety and excavation-induced deformations. The results obtained for both undrained and fully coupled flow deformation analyses prove that CSM panels can be constructed by mixing slag lime and CIS to act as retaining walls to allow for deep excavation in CIS.
- Description: Doctor of Philosophy
- Authors: Ranjbar Pouya, Kaveh
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Coode Island Silt (CIS) is one of the predominant geological units in Melbourne, Australia. Having high compressibility and low shear strength, CIS is considered a problematic soft soil that challenges the construction of infrastructure in the region. To tackle such challenges, one practical approach is the application of ground improvement techniques such as in situ soil mixing. This PhD study focuses on the application of Cutter Soil Mixing (CSM) for the construction of excavation retaining walls in CIS. Although cement is widely used in most CSM projects, this study investigates the suitability of different lime types available in the Australian market as a potential alternative to cement for the stabilisation of CIS. To investigate the effect of lime stabilisation, a comprehensive geotechnical characterisation of untreated and lime treated CIS is performed. Four different lime types are used: agricultural lime, quicklime, hydrated lime and slag lime. Based on the results obtained from strength tests, slag lime was found to be the most effective among the four types that were tested. The optimum slag lime to CIS ratio is then found for the construction of retaining walls in CIS. Having the geotechnical characterisation of untreated and treated CIS from the laboratory experiments, a series of two-dimensional and three-dimensional finite element method (FEM) analyses were conducted to investigate the applicability and reliability of the selected mixing ratio for the construction of CSM excavation retaining walls in CIS. A nonlinear constitutive soil model was employed, calibrated and verified to be used in FEM analyses to investigate both the stability factor of safety and excavation-induced deformations. The results obtained for both undrained and fully coupled flow deformation analyses prove that CSM panels can be constructed by mixing slag lime and CIS to act as retaining walls to allow for deep excavation in CIS.
- Description: Doctor of Philosophy
Negotiating policy - renegotiating practice : Understanding language, literacy and numeracy teachers' professional identities within discursive weather systems
- Authors: Krusche, Julianne
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: This thesis examines the professional identities of teachers in light of Australian vocational educational policy reform since the late 1980s. Although the reform has been national, this study is located within Victoria. It is specifically interested in the professional identities of Language, Literacy and Numeracy (LLN) teachers who work within the Vocational Education and Training sector. This study brings the voices of LLN specialists to the forefront. Poststructural theory, with particular emphasis on the work of Michel Foucault and narrative inquiry, is used to make sense of these voices, collectively known as the ‘voice of practice’. This study treats professional identity as a multiple term encompassing a range of assigned roles and chosen identities; as such, it argues that professional identity should be seen as fluid and dynamic and as something that continues to evolve. The teachers involved in this study actively negotiated various discourses related to the shaping of professional identity. The effects of this were threefold: LLN teachers ascribed to certain identity positions in line with government policy and institute directives; they preserved other identities; and they forged new identities based on opportunism and a resistance to policy discourse. While there has been a decline in the Adult and Community Education voice in policy development, within practice, this study found that teachers have retained a voice through the maintenance and creation of teaching practices that sit outside policy. Further, this study found that although numerous stakeholders invest in the provision of LLN, it is learners who hold a lot of the power; indeed, the needs of learners, feedback from learners and their transformative learning experiences are the primary drivers in teacher motivation and identity. Finally, this study found a misalignment between policy discourse and the voice of practice that requires attention if LLN policies are to be successfully implemented.
- Description: Doctor of Philosophy
- Authors: Krusche, Julianne
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: This thesis examines the professional identities of teachers in light of Australian vocational educational policy reform since the late 1980s. Although the reform has been national, this study is located within Victoria. It is specifically interested in the professional identities of Language, Literacy and Numeracy (LLN) teachers who work within the Vocational Education and Training sector. This study brings the voices of LLN specialists to the forefront. Poststructural theory, with particular emphasis on the work of Michel Foucault and narrative inquiry, is used to make sense of these voices, collectively known as the ‘voice of practice’. This study treats professional identity as a multiple term encompassing a range of assigned roles and chosen identities; as such, it argues that professional identity should be seen as fluid and dynamic and as something that continues to evolve. The teachers involved in this study actively negotiated various discourses related to the shaping of professional identity. The effects of this were threefold: LLN teachers ascribed to certain identity positions in line with government policy and institute directives; they preserved other identities; and they forged new identities based on opportunism and a resistance to policy discourse. While there has been a decline in the Adult and Community Education voice in policy development, within practice, this study found that teachers have retained a voice through the maintenance and creation of teaching practices that sit outside policy. Further, this study found that although numerous stakeholders invest in the provision of LLN, it is learners who hold a lot of the power; indeed, the needs of learners, feedback from learners and their transformative learning experiences are the primary drivers in teacher motivation and identity. Finally, this study found a misalignment between policy discourse and the voice of practice that requires attention if LLN policies are to be successfully implemented.
- Description: Doctor of Philosophy
Of railways, wine and flowers : A biography of George William Knight
- Authors: Hunter, Robyn
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: This thesis examines misunderstandings surrounding class and reveals Knight to have been a member of the petty bourgeoisie - aspirational, yet understanding and empathetic towards working people. Knight was an erudite, respectable gentleman, an intellectual, a man of culture, committed to liberal values and the code of the gentleman, and a defender of the oppressed. Highlighted in this study are the high tides, low ebbs and pivotal turning points that beset Knight's life. A noted writer and raconteur, he was an influential figure in Bendigo and the colony. Covering more than ninety years, Knight's story encompasses London 1832-56, early colonial Victoria to 1923, and shines a light on one of the many oft-forgotten people who helped to build the colony.
- Description: Doctor of Philosophy
- Description: The title page photograph
- Authors: Hunter, Robyn
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: This thesis examines misunderstandings surrounding class and reveals Knight to have been a member of the petty bourgeoisie - aspirational, yet understanding and empathetic towards working people. Knight was an erudite, respectable gentleman, an intellectual, a man of culture, committed to liberal values and the code of the gentleman, and a defender of the oppressed. Highlighted in this study are the high tides, low ebbs and pivotal turning points that beset Knight's life. A noted writer and raconteur, he was an influential figure in Bendigo and the colony. Covering more than ninety years, Knight's story encompasses London 1832-56, early colonial Victoria to 1923, and shines a light on one of the many oft-forgotten people who helped to build the colony.
- Description: Doctor of Philosophy
- Description: The title page photograph
Physical activity promotion in physiotherapy practice
- Authors: Kunstler, Breanne
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Australian physiotherapists promote non-treatment physical activity, which is physical activity used to improve or maintain general health, to patients with musculoskeletal conditions. However, it is unclear how physiotherapists promote non-treatment physical activity and the behaviour change techniques they use to do this. This thesis used four studies to: (i) review the efficacy of physiotherapist-led physical activity interventions; (ii) investigate the factors that influence physiotherapists’ choice to promote non-treatment physical activity; and (iii) identify the behaviour change techniques that private practice and outpatient physiotherapists use to promote non-treatment physical activity. Two systematic reviews identified that physiotherapist-led physical activity interventions are efficacious. However, effects were small and not maintained. Additionally, physiotherapists only used a small number of behaviour change techniques when promoting physical activity. National survey and interview studies were used to identify the factors that influence physiotherapists’ choice to promote non-treatment physical activity. The survey found that having poor knowledge of how to promote non-treatment physical activity, prioritising other patient problems before non-treatment physical activity and using promotion methods that were not compatible with daily practice significantly and independently reduced the odds of physiotherapists promoting non-treatment physical activity. Interpretative phenomenological analysis was used to design interviews that showed that having a perceived inability to motivate an unmotivated patient and believing that patients expect hands-on therapy instead of non-treatment physical activity promotion complicated treatment choices. The behaviour change techniques Australian physiotherapists used to promote non-treatment physical activity were compared to those used to encourage adherence to rehabilitation exercises in the survey too. The survey found that physiotherapists used similar behaviour change techniques to promote non-treatment physical activity and encourage adherence to rehabilitation exercises. This thesis provides clinicians and researchers with an understanding of the factors that influence Australian physiotherapists’ decision to promote non-treatment physical activity and the behaviour change techniques they use.
- Description: Doctor of Philosophy
- Authors: Kunstler, Breanne
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Australian physiotherapists promote non-treatment physical activity, which is physical activity used to improve or maintain general health, to patients with musculoskeletal conditions. However, it is unclear how physiotherapists promote non-treatment physical activity and the behaviour change techniques they use to do this. This thesis used four studies to: (i) review the efficacy of physiotherapist-led physical activity interventions; (ii) investigate the factors that influence physiotherapists’ choice to promote non-treatment physical activity; and (iii) identify the behaviour change techniques that private practice and outpatient physiotherapists use to promote non-treatment physical activity. Two systematic reviews identified that physiotherapist-led physical activity interventions are efficacious. However, effects were small and not maintained. Additionally, physiotherapists only used a small number of behaviour change techniques when promoting physical activity. National survey and interview studies were used to identify the factors that influence physiotherapists’ choice to promote non-treatment physical activity. The survey found that having poor knowledge of how to promote non-treatment physical activity, prioritising other patient problems before non-treatment physical activity and using promotion methods that were not compatible with daily practice significantly and independently reduced the odds of physiotherapists promoting non-treatment physical activity. Interpretative phenomenological analysis was used to design interviews that showed that having a perceived inability to motivate an unmotivated patient and believing that patients expect hands-on therapy instead of non-treatment physical activity promotion complicated treatment choices. The behaviour change techniques Australian physiotherapists used to promote non-treatment physical activity were compared to those used to encourage adherence to rehabilitation exercises in the survey too. The survey found that physiotherapists used similar behaviour change techniques to promote non-treatment physical activity and encourage adherence to rehabilitation exercises. This thesis provides clinicians and researchers with an understanding of the factors that influence Australian physiotherapists’ decision to promote non-treatment physical activity and the behaviour change techniques they use.
- Description: Doctor of Philosophy
Semantic manipulation and business context in big data analytics
- Authors: Dinh, Loan
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Business organisations receive a huge amount of data from many sources every day. These data are known as big data. Since they are mostly unstructured, big data creates a complex problem of how to capture, manage, analyse and then derive meaningful information from them. To deal with the challenges that big data has brought, this research proposes a new technique in big data analytics in the business area to integrate semantically meaningful information relevant to textual queries and business context. To achieve this aim, this study makes three major related contributions. Firstly, the relationship between business processes and strategies is established using the concept of a rule-based inference model via facts and annotations. This relationship is required to determine the importance of a big data query for a business organisation. Secondly, we introduce approaches to determine the significance level of a query, by incorporating the processstrategy relationship, process contributions and priority of business strategies. Thirdly, the proposed data analytic technique embeds business context into the bedrock of data collection and analysis process. The first two contributions were implemented using Python programming language including the Pyke package (Pyke is built in the Python environment and has an artificial intelligence tool for the development of expert systems) and their performances were analysed based on a business use case. The last contribution was implemented mainly in the Hadoop and Java programs. Results show that the first contribution successfully establishes the processstrategy relationship, the second calculates the significance level of a query in relation to a business organisation, while the third reveals the huge impact of query significance level and business context on big data collection and captures deep business insights.
- Description: Doctor of Philosophy
- Authors: Dinh, Loan
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Business organisations receive a huge amount of data from many sources every day. These data are known as big data. Since they are mostly unstructured, big data creates a complex problem of how to capture, manage, analyse and then derive meaningful information from them. To deal with the challenges that big data has brought, this research proposes a new technique in big data analytics in the business area to integrate semantically meaningful information relevant to textual queries and business context. To achieve this aim, this study makes three major related contributions. Firstly, the relationship between business processes and strategies is established using the concept of a rule-based inference model via facts and annotations. This relationship is required to determine the importance of a big data query for a business organisation. Secondly, we introduce approaches to determine the significance level of a query, by incorporating the processstrategy relationship, process contributions and priority of business strategies. Thirdly, the proposed data analytic technique embeds business context into the bedrock of data collection and analysis process. The first two contributions were implemented using Python programming language including the Pyke package (Pyke is built in the Python environment and has an artificial intelligence tool for the development of expert systems) and their performances were analysed based on a business use case. The last contribution was implemented mainly in the Hadoop and Java programs. Results show that the first contribution successfully establishes the processstrategy relationship, the second calculates the significance level of a query in relation to a business organisation, while the third reveals the huge impact of query significance level and business context on big data collection and captures deep business insights.
- Description: Doctor of Philosophy
Spatial epidemiological investigation of sport and leisure injuries in Victoria, Australia
- Authors: Singh, Himalaya
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Sport and leisure injuries are recognised as a public health issue in Australia. Despite the many health benefits associated with sport and leisure participation, there is a risk of sustaining injury during participation. To keep Australia active, there is a critical need to prevent injury occurrence. Epidemiological investigations in sport and leisure injuries have been largely examined by grouping of sports, age groups, sex and level of play. In addition, intrinsic (person-level) factors have been considered, such as strength, flexibility or previous injury history. These factors may not be sufficient to identify injury burden or prevent an increase in injury incidences. In the broader injury literature (e.g., road traffic crashes or drowning), it is known that injuries often cluster within specific places (i.e., road intersections or bodies of water). These specific geographic locations may also relate to sport and leisure injuries (e.g., sports grounds or facilities). Similarly, population-level factors such as socio-economic status or cultural groups within an area could influence the types of sports and leisure activities people participate in and consequently, the injuries that occur. A review presented in this PhD thesis revealed that there is very limited sport and leisure injury epidemiological information from a geographical perspective. To address this gap, and determine whether there is a spatial pattern in sport/leisure injuries, the aim of this PhD was to examine the geospatial distribution of sport/leisure injury hospitalisations and their association with a broad range of social and economic characteristics. This thesis uses spatial epidemiological methods to answer questions such as ‘Where do sports and leisure injuries occur?’ and ‘In whom do sports/leisure injuries occur?’ The main chapters present the results of the application of spatial epidemiological methods to describe the problem, to test hypotheses and to explore associations with possible explanatory variables. The findings showed a significant variation across metropolitan, regional and rural areas in the pattern and clustering of injuries when examining different sports, age groups and other variables such as education level. A secondary aim of this thesis was to consider the dissemination of sport and injury epidemiological data. As emphasised in the literature, there is limited spatial epidemiological information available to decision-makers and key stakeholders. At best, descriptive maps might be included in a report or research paper. However, these are static and limited to the results that the author chooses to present. Therefore, an important output from this PhD is a web-GIS application that has been specifically built to enable the exploratory analysis of sport/leisure injuries in Victoria. Sport and leisure injury prevention strategies and policy development relies on information about where, when, to whom and how sport/leisure injuries occur. This thesis demonstrates that a spatial epidemiological approach is an important and novel way to address epidemiological questions from a geographical perspective.
- Description: Doctor of Philosophy
- Authors: Singh, Himalaya
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Sport and leisure injuries are recognised as a public health issue in Australia. Despite the many health benefits associated with sport and leisure participation, there is a risk of sustaining injury during participation. To keep Australia active, there is a critical need to prevent injury occurrence. Epidemiological investigations in sport and leisure injuries have been largely examined by grouping of sports, age groups, sex and level of play. In addition, intrinsic (person-level) factors have been considered, such as strength, flexibility or previous injury history. These factors may not be sufficient to identify injury burden or prevent an increase in injury incidences. In the broader injury literature (e.g., road traffic crashes or drowning), it is known that injuries often cluster within specific places (i.e., road intersections or bodies of water). These specific geographic locations may also relate to sport and leisure injuries (e.g., sports grounds or facilities). Similarly, population-level factors such as socio-economic status or cultural groups within an area could influence the types of sports and leisure activities people participate in and consequently, the injuries that occur. A review presented in this PhD thesis revealed that there is very limited sport and leisure injury epidemiological information from a geographical perspective. To address this gap, and determine whether there is a spatial pattern in sport/leisure injuries, the aim of this PhD was to examine the geospatial distribution of sport/leisure injury hospitalisations and their association with a broad range of social and economic characteristics. This thesis uses spatial epidemiological methods to answer questions such as ‘Where do sports and leisure injuries occur?’ and ‘In whom do sports/leisure injuries occur?’ The main chapters present the results of the application of spatial epidemiological methods to describe the problem, to test hypotheses and to explore associations with possible explanatory variables. The findings showed a significant variation across metropolitan, regional and rural areas in the pattern and clustering of injuries when examining different sports, age groups and other variables such as education level. A secondary aim of this thesis was to consider the dissemination of sport and injury epidemiological data. As emphasised in the literature, there is limited spatial epidemiological information available to decision-makers and key stakeholders. At best, descriptive maps might be included in a report or research paper. However, these are static and limited to the results that the author chooses to present. Therefore, an important output from this PhD is a web-GIS application that has been specifically built to enable the exploratory analysis of sport/leisure injuries in Victoria. Sport and leisure injury prevention strategies and policy development relies on information about where, when, to whom and how sport/leisure injuries occur. This thesis demonstrates that a spatial epidemiological approach is an important and novel way to address epidemiological questions from a geographical perspective.
- Description: Doctor of Philosophy
Stay or go? Young people’s agency and mobility in and out of small towns
- Authors: Parkin, Ember
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: This doctoral thesis examines young people’s place attachments in two small Victorian towns. This qualitative ethnographic study uses auto-driven photo-elicitation to understand young people’s sense of place and futures in their home towns of Castlemaine and Maryborough. These case study towns are of a similar size, geography and heritage fabric. However, they are home to starkly different social indicators and economic policy contexts. The study seeks to understand how the cultural features of small towns affect young people’s place attachment and also how place relationships might subsequently affect young people’s sense of futures through their desired and intended locations and aspirations. To achieve this, the thesis explores young people’s social constructions of place. The photoelicitation method enables close attention to be paid to young people’s engagement with their home towns. This thesis argues that agency or lack of agency is a significant factor in strengthening or diminishing young people’s place attachments. Previous research suggests that one result of place attachment is that people will seek to remain being in a place. For young people in this study there appears to be an inverse relationship. Young people who had a broad and holistic sense of place engagement and attachment also had a broad sense of future possibilities and thus, intended to leave their home towns in pursuit of personal growth and education. Whereas young people who had a more limited sense of attachment or engagement had a narrower sense of future possibilities and were less likely to desire to leave their home town. The study contributes to knowledge about the ways in which place engagement can affect young people’s social and physical mobility.
- Description: Doctor of Philosophy
- Authors: Parkin, Ember
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: This doctoral thesis examines young people’s place attachments in two small Victorian towns. This qualitative ethnographic study uses auto-driven photo-elicitation to understand young people’s sense of place and futures in their home towns of Castlemaine and Maryborough. These case study towns are of a similar size, geography and heritage fabric. However, they are home to starkly different social indicators and economic policy contexts. The study seeks to understand how the cultural features of small towns affect young people’s place attachment and also how place relationships might subsequently affect young people’s sense of futures through their desired and intended locations and aspirations. To achieve this, the thesis explores young people’s social constructions of place. The photoelicitation method enables close attention to be paid to young people’s engagement with their home towns. This thesis argues that agency or lack of agency is a significant factor in strengthening or diminishing young people’s place attachments. Previous research suggests that one result of place attachment is that people will seek to remain being in a place. For young people in this study there appears to be an inverse relationship. Young people who had a broad and holistic sense of place engagement and attachment also had a broad sense of future possibilities and thus, intended to leave their home towns in pursuit of personal growth and education. Whereas young people who had a more limited sense of attachment or engagement had a narrower sense of future possibilities and were less likely to desire to leave their home town. The study contributes to knowledge about the ways in which place engagement can affect young people’s social and physical mobility.
- Description: Doctor of Philosophy
Teachers' emotional intelligence as a predisposition for discrimiation against students with severe emotional and behavioural disorders
- Authors: Metaxas, Melinda
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Students with Emotional Behavioural Disorders (EBD) are among the most challenging students to teach. Personality Trait Theory predicts teachers’ level of Emotional Intelligence (EI) would affect their cognitive-affective-behavioural reactions towards students with EBDs, and influence level of academic achievement and difficult behaviour of these students. This research explores which teachers are more predisposed to discriminate against EBD students, and identifies the most ‘effective’, supportive EI teacher traits. Underlying psychological processes, such as genetic EI make-up of teachers, may prove to be most valuable in determining whether more practical strategies for dealing with students’ behaviour/emotions are effectively applied and successful. An Attribution Model framework helped assess teacher reactions towards students. Two hundred and sixty one teachers from 51 Victorian schools participated in the study by completing self-report questionnaires, including the Trait Emotional Intelligence Questionnaire. A quantitative survey methodology used vignettes, with each school contact person randomly giving 50/50 surveys to their teachers (depicting a student with either mild or severe EBD symptoms). Pathway analysis revealed that teachers with higher EI reported less stigmatising and punitive intentions and likely greater helping behaviours. A new EI Process Model of Stigmatisation was proposed to measure teacher reactions through an affective-cognitive-behavioural sequence, rather than a cognitive-affective-behavioural sequence. Teachers’ EI levels also related to their own levels of psychological distress and/or compassion stress, which influenced likely helping or punitive outcomes. Despite behavioural severity of EBD students, teachers higher in EI still indicate more supportive helping behaviours. Specifically-identified ‘ideal’ teacher EI traits should lead to greater helping and be psychologically beneficial to both students and teachers. These results assisted development of an assessment tool (ASET – Assessment Screen for Emotionally Intelligent Teachers), which lays a sound foundation for schools and others to profile or recruit teachers with best ‘qualities’ to effectively teach students EBD students.
- Description: Doctor of Philosophy
- Authors: Metaxas, Melinda
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Students with Emotional Behavioural Disorders (EBD) are among the most challenging students to teach. Personality Trait Theory predicts teachers’ level of Emotional Intelligence (EI) would affect their cognitive-affective-behavioural reactions towards students with EBDs, and influence level of academic achievement and difficult behaviour of these students. This research explores which teachers are more predisposed to discriminate against EBD students, and identifies the most ‘effective’, supportive EI teacher traits. Underlying psychological processes, such as genetic EI make-up of teachers, may prove to be most valuable in determining whether more practical strategies for dealing with students’ behaviour/emotions are effectively applied and successful. An Attribution Model framework helped assess teacher reactions towards students. Two hundred and sixty one teachers from 51 Victorian schools participated in the study by completing self-report questionnaires, including the Trait Emotional Intelligence Questionnaire. A quantitative survey methodology used vignettes, with each school contact person randomly giving 50/50 surveys to their teachers (depicting a student with either mild or severe EBD symptoms). Pathway analysis revealed that teachers with higher EI reported less stigmatising and punitive intentions and likely greater helping behaviours. A new EI Process Model of Stigmatisation was proposed to measure teacher reactions through an affective-cognitive-behavioural sequence, rather than a cognitive-affective-behavioural sequence. Teachers’ EI levels also related to their own levels of psychological distress and/or compassion stress, which influenced likely helping or punitive outcomes. Despite behavioural severity of EBD students, teachers higher in EI still indicate more supportive helping behaviours. Specifically-identified ‘ideal’ teacher EI traits should lead to greater helping and be psychologically beneficial to both students and teachers. These results assisted development of an assessment tool (ASET – Assessment Screen for Emotionally Intelligent Teachers), which lays a sound foundation for schools and others to profile or recruit teachers with best ‘qualities’ to effectively teach students EBD students.
- Description: Doctor of Philosophy
Tertiary student connectedness : Intervention influence on student connectedness as measured in health and academic behaviours of regional tertiary students
- Authors: Young, Patricia
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: At a time when university student diversity is heightened and when national government regulations have shifted their quality focus from support improvements to student achievement, little is known about the mechanism of the latter. University efforts to support quality, caring interactions between students and staff, known as connectedness, are currently favoured, and this thesis examines connectedness, mood, emotional well-being and academic behaviours of commencing students at a regional Australian university. This is done through the lens of a first semester intervention, known as Tertiary Learning Communities (TLC). Drawing on existing connectedness research, across both school and university settings and guided by a whole-school framework, details of the establishment of a suitable working party, the development of survey, pilot and intervention activities, and monitoring the ability of a cross-campus intervention to influence student connectedness and behaviour markers, are highlighted to advance further understanding of the mechanics of connectedness in a university setting. The developed survey, which collected data from undergraduate students early in their first semester, effectively captured perceptions of connectedness across a broad range of sources in addition to their mood, emotional well-being and academic student behaviours. Paired sample-tests assessed connectedness changes, and chi square analysis assessed behaviour changes when comparing the experimental and control groups on two occasions. A single intervention aligned to a first year core unit to support academic and social interactions, was shown to be ineffective in enhancing student connectedness during the semester of the intervention or the semester following the intervention. However, the level of connectedness decreases measured across ‘personal’, ‘other students’ and ‘lecturers’ were indeed significant, as was the finding that connectedness decreases for intervention participants exceeded the decreases of the control group. Furthermore, mood and emotional well-being challenges and the slow emergence of academic behaviours were also revealed. These findings provided support for future inclusive student support initiatives, maintained the involvement of working party members and extended support beyond the first semester to across first year.
- Description: Doctor of Philosophy
- Authors: Young, Patricia
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: At a time when university student diversity is heightened and when national government regulations have shifted their quality focus from support improvements to student achievement, little is known about the mechanism of the latter. University efforts to support quality, caring interactions between students and staff, known as connectedness, are currently favoured, and this thesis examines connectedness, mood, emotional well-being and academic behaviours of commencing students at a regional Australian university. This is done through the lens of a first semester intervention, known as Tertiary Learning Communities (TLC). Drawing on existing connectedness research, across both school and university settings and guided by a whole-school framework, details of the establishment of a suitable working party, the development of survey, pilot and intervention activities, and monitoring the ability of a cross-campus intervention to influence student connectedness and behaviour markers, are highlighted to advance further understanding of the mechanics of connectedness in a university setting. The developed survey, which collected data from undergraduate students early in their first semester, effectively captured perceptions of connectedness across a broad range of sources in addition to their mood, emotional well-being and academic student behaviours. Paired sample-tests assessed connectedness changes, and chi square analysis assessed behaviour changes when comparing the experimental and control groups on two occasions. A single intervention aligned to a first year core unit to support academic and social interactions, was shown to be ineffective in enhancing student connectedness during the semester of the intervention or the semester following the intervention. However, the level of connectedness decreases measured across ‘personal’, ‘other students’ and ‘lecturers’ were indeed significant, as was the finding that connectedness decreases for intervention participants exceeded the decreases of the control group. Furthermore, mood and emotional well-being challenges and the slow emergence of academic behaviours were also revealed. These findings provided support for future inclusive student support initiatives, maintained the involvement of working party members and extended support beyond the first semester to across first year.
- Description: Doctor of Philosophy
The acute effects of aerobic exercise on Leukocyte Telomere biology
- Authors: Chilton, Warrick
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Doctor of Philosophy
- Description: Habitual exercise is unequivocally associated with decreased all-cause mortality and morbidity. Despite the strength of the association, a large part of the decreased risk is physiologically unaccounted for. Accumulating evidence indicates that leukocyte telomere length (LTL) may be one such explanatory mechanism. Telomeres are specialized deoxyribonucleic acid (DNA) sequences located at chromosomal ends where they protect the genomic DNA from enzymatic degradation. Excessive and/or premature telomere shortening in leukocytes is associated with a host of chronic diseases and impaired immune function. Observational associations exist between LTL and habitual physical activity/exercise in multiple cohorts. However, correlation does not imply causal story and the underpinning mechanisms behind the association are unclear. The current consensus is that long-term exercise-induced reductions in oxidative stress and inflammation mediate the association. The acute dynamics of telomere biology are poorly understood; however, a growing body of evidence suggests that telomeres may be amenable to acute modulation via expression of telomereassociated genes and microRNAs. Accordingly, the overarching aim of this thesis was to characterize the acute effects of aerobic exercise on leukocyte telomere biology.
- Authors: Chilton, Warrick
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Doctor of Philosophy
- Description: Habitual exercise is unequivocally associated with decreased all-cause mortality and morbidity. Despite the strength of the association, a large part of the decreased risk is physiologically unaccounted for. Accumulating evidence indicates that leukocyte telomere length (LTL) may be one such explanatory mechanism. Telomeres are specialized deoxyribonucleic acid (DNA) sequences located at chromosomal ends where they protect the genomic DNA from enzymatic degradation. Excessive and/or premature telomere shortening in leukocytes is associated with a host of chronic diseases and impaired immune function. Observational associations exist between LTL and habitual physical activity/exercise in multiple cohorts. However, correlation does not imply causal story and the underpinning mechanisms behind the association are unclear. The current consensus is that long-term exercise-induced reductions in oxidative stress and inflammation mediate the association. The acute dynamics of telomere biology are poorly understood; however, a growing body of evidence suggests that telomeres may be amenable to acute modulation via expression of telomereassociated genes and microRNAs. Accordingly, the overarching aim of this thesis was to characterize the acute effects of aerobic exercise on leukocyte telomere biology.
The contribution of silverfish (insecta: zygentoma) to Australian invertebrate biodiversity and endemism
- Authors: Smith, Graeme
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Silverfish (Order Zygentoma) are quite abundant in Australia but have been largely overlooked. This thesis examines the biodiversity of the Australian fauna at the level of genus, describing at least one representative species from each named genus and some new genera. The endemism of the fauna is evaluated and likely zoogeographic origins proposed. Over 4000 specimens were examined, either collected by the author, borrowed from or examined within museum collections or supplied by organisations and individuals conducting fauna surveys. Twenty- seven new native species are described and two redescribed, bringing the number of named species recorded in Australia to 74. Five new genera are described and four additional genera recorded in Australia for the first time including autochthonous representatives of three subfamilies previously unrecognised as native to Australia (Acrotelsatinae, Lepismatinae and Coletiniinae). No representatives of the families Maindroniidae, Tricholepidiidae and Protrinemuridae were found. The subfamily Acrotelsatinae was redefined following a revision of the enigmatic genus Anisolepisma Paclt, 1967 with the unique structure of the thoracic sterna identified as diagnostic for the subfamily. Contrary to existing opinion, it is suggested that this is a fundamentally different and plesiomorphic character, rather than an apomorphic reduction of the free thoracic sterna. A monograph of the Australian Zygentoma is presented, including a summary of the biology of the order, a key to and diagnoses of the genera, as well as information on the known habitat and distribution of each genus and a discussion of their zoogeography. At the suprageneric level the fauna is less diverse than seen in other zoogeographic regions but appears to be rich in the number of species. The fauna displays a high degree of endemism with 91% of described species and 52% of the genera known only from Australia. Some genera appear to be ancient and probably represent a Pangean element in the Australian fauna. Others appear to have emerged in the late Jurassic when Africa was still joined to Gondwana, while some may have appeared in the Cretaceous or Palaeocene when Australia and South America were connected to Gondwana. More recent links with the Asian fauna are limited and there appears to be no widespread highly mobile global species other than the six introduced anthropophilic species. Maps of the worldwide distribution records extracted from the taxonomic literature are used to discuss the zoogeography of the subfamilies and tribes present in Australia. Molecular data using two mitochondrial genes (16S and COI) as well as a nuclear gene (28S) were compared with detailed morphological and morphometric analysis to examine populations initially determined as Heterolepisma sclerophylla or close to it. Distances of 0.9– 1.8% or greater in 28S, and 7.2–14% in COI were associated with morphologically distinct species. A southern Queensland population was found to be genetically, morphometrically and morphologically very distinct from those collected in NSW and was described as new (Heterolepisma sp. B). Six well-defined barcode clusters (“lineages”) were identified within the NSW populations, each with >4% divergence in COI sequences and each geographically restricted. Intracluster divergences are also large, and despite the well-supported phylogeny no clear “barcode gap” (distinction between intracluster and intercluster distances) was found for three of the six NSW populations. The 28S data distinguished only four of the six COI clusters from NSW with essentially no variation within each cluster. The 28S data generally aligned well with morphological evidence, clearly identifying Heterolepisma sp. B as a distinct species, and supporting also the description of Heterolepisma sp. A even though it only appears to differ from H. sclerophylla in the number of styli. Similar genetic distances are observed in 28S data for H. sclerophylla populations from North Nowra, Glenbrook/Burralow/Nattai and Megalong, however the Broulee and Wellington populations have identical 28S sequences. The low levels of variation in 28S sequences between NSW populations accord with the lack of unambiguous morphological differences.
- Description: Doctor of Philosophy
- Authors: Smith, Graeme
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Silverfish (Order Zygentoma) are quite abundant in Australia but have been largely overlooked. This thesis examines the biodiversity of the Australian fauna at the level of genus, describing at least one representative species from each named genus and some new genera. The endemism of the fauna is evaluated and likely zoogeographic origins proposed. Over 4000 specimens were examined, either collected by the author, borrowed from or examined within museum collections or supplied by organisations and individuals conducting fauna surveys. Twenty- seven new native species are described and two redescribed, bringing the number of named species recorded in Australia to 74. Five new genera are described and four additional genera recorded in Australia for the first time including autochthonous representatives of three subfamilies previously unrecognised as native to Australia (Acrotelsatinae, Lepismatinae and Coletiniinae). No representatives of the families Maindroniidae, Tricholepidiidae and Protrinemuridae were found. The subfamily Acrotelsatinae was redefined following a revision of the enigmatic genus Anisolepisma Paclt, 1967 with the unique structure of the thoracic sterna identified as diagnostic for the subfamily. Contrary to existing opinion, it is suggested that this is a fundamentally different and plesiomorphic character, rather than an apomorphic reduction of the free thoracic sterna. A monograph of the Australian Zygentoma is presented, including a summary of the biology of the order, a key to and diagnoses of the genera, as well as information on the known habitat and distribution of each genus and a discussion of their zoogeography. At the suprageneric level the fauna is less diverse than seen in other zoogeographic regions but appears to be rich in the number of species. The fauna displays a high degree of endemism with 91% of described species and 52% of the genera known only from Australia. Some genera appear to be ancient and probably represent a Pangean element in the Australian fauna. Others appear to have emerged in the late Jurassic when Africa was still joined to Gondwana, while some may have appeared in the Cretaceous or Palaeocene when Australia and South America were connected to Gondwana. More recent links with the Asian fauna are limited and there appears to be no widespread highly mobile global species other than the six introduced anthropophilic species. Maps of the worldwide distribution records extracted from the taxonomic literature are used to discuss the zoogeography of the subfamilies and tribes present in Australia. Molecular data using two mitochondrial genes (16S and COI) as well as a nuclear gene (28S) were compared with detailed morphological and morphometric analysis to examine populations initially determined as Heterolepisma sclerophylla or close to it. Distances of 0.9– 1.8% or greater in 28S, and 7.2–14% in COI were associated with morphologically distinct species. A southern Queensland population was found to be genetically, morphometrically and morphologically very distinct from those collected in NSW and was described as new (Heterolepisma sp. B). Six well-defined barcode clusters (“lineages”) were identified within the NSW populations, each with >4% divergence in COI sequences and each geographically restricted. Intracluster divergences are also large, and despite the well-supported phylogeny no clear “barcode gap” (distinction between intracluster and intercluster distances) was found for three of the six NSW populations. The 28S data distinguished only four of the six COI clusters from NSW with essentially no variation within each cluster. The 28S data generally aligned well with morphological evidence, clearly identifying Heterolepisma sp. B as a distinct species, and supporting also the description of Heterolepisma sp. A even though it only appears to differ from H. sclerophylla in the number of styli. Similar genetic distances are observed in 28S data for H. sclerophylla populations from North Nowra, Glenbrook/Burralow/Nattai and Megalong, however the Broulee and Wellington populations have identical 28S sequences. The low levels of variation in 28S sequences between NSW populations accord with the lack of unambiguous morphological differences.
- Description: Doctor of Philosophy
The influence of controlled fire on the mobilization of Potentially Toxic Elements (PTE) in a mined landscape : Implications for land management and environmental health
- Authors: Abraham, Joji
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Controlled fires conducted in fire prone areas are an efficient and economic option to reduce the frequency and intensity of wild fires that result in damage to human property, infrastructure and ecosystems. However, in a similar way to wild fires, controlled fires affect many of the physical and bio-geochemical properties of the forest soil, and may remobilize Potentially Toxic Elements (PTE) from vegetation and soil organic matter. The objective of this study is to investigate the mobilization of PTEs in a mined landscape after a controlled fire and to describe their temporal variations in concentrations. Soil samples were collected two days before and two days after the controlled fire, at the end of each season, and after a major rainfall in September 2016, from a legacy mine site in Maldon, Central Victoria, Australia, and analysed for PTE concentrations. The results revealed PTE mobility after the controlled fire, and most of the PTEs (As, Cd, Cu, Mn, and Zn) evidenced an increase in concentration (1.2, 1.5, 1.1, 2.9, and 1.7 times respectively) and other PTEs (Hg, Cr and Pb) shown a decrease (1.4, 1.1 and 1.1 times respectively) immediately after the fire. The increase in PTE concentration immediately after the fire is postulated to be associated with the addition of PTE enriched ash to the soil and the reduction is due to the volatilization of elements during fire. The PTEs, which increased their concentrations immediately after the fire show a temporal decrease in concentration in the post-fire soil environment due to the removal of ash and surface soil by rainfall runoff, leaching and wind activity. However, Hg shows an increase in concentration after the major rainfall event. Although, median concentrations of As, Hg, Pb, Cu and Zn exceeded the Australian and Victorian top soil averages, only As and Hg are considered to be a risk to human and aquatic ecosystems health due to their elevated concentration and toxicity. Climate change and the resulting projection for increased forest fire frequency illustrates a growing concern given the expected concomitant increase in PTE mobilization. Preparing appropriate land and water management strategies, and addressing environmental health practice and policy, specifically at the legacy mining areas require a review. This study highlights the significant risk these sites pose.
- Description: Doctor of Philosophy
- Authors: Abraham, Joji
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Controlled fires conducted in fire prone areas are an efficient and economic option to reduce the frequency and intensity of wild fires that result in damage to human property, infrastructure and ecosystems. However, in a similar way to wild fires, controlled fires affect many of the physical and bio-geochemical properties of the forest soil, and may remobilize Potentially Toxic Elements (PTE) from vegetation and soil organic matter. The objective of this study is to investigate the mobilization of PTEs in a mined landscape after a controlled fire and to describe their temporal variations in concentrations. Soil samples were collected two days before and two days after the controlled fire, at the end of each season, and after a major rainfall in September 2016, from a legacy mine site in Maldon, Central Victoria, Australia, and analysed for PTE concentrations. The results revealed PTE mobility after the controlled fire, and most of the PTEs (As, Cd, Cu, Mn, and Zn) evidenced an increase in concentration (1.2, 1.5, 1.1, 2.9, and 1.7 times respectively) and other PTEs (Hg, Cr and Pb) shown a decrease (1.4, 1.1 and 1.1 times respectively) immediately after the fire. The increase in PTE concentration immediately after the fire is postulated to be associated with the addition of PTE enriched ash to the soil and the reduction is due to the volatilization of elements during fire. The PTEs, which increased their concentrations immediately after the fire show a temporal decrease in concentration in the post-fire soil environment due to the removal of ash and surface soil by rainfall runoff, leaching and wind activity. However, Hg shows an increase in concentration after the major rainfall event. Although, median concentrations of As, Hg, Pb, Cu and Zn exceeded the Australian and Victorian top soil averages, only As and Hg are considered to be a risk to human and aquatic ecosystems health due to their elevated concentration and toxicity. Climate change and the resulting projection for increased forest fire frequency illustrates a growing concern given the expected concomitant increase in PTE mobilization. Preparing appropriate land and water management strategies, and addressing environmental health practice and policy, specifically at the legacy mining areas require a review. This study highlights the significant risk these sites pose.
- Description: Doctor of Philosophy
Visiting Friends and Relatives (VFR) travel in Australia : An examination of the role of VFR hosts
- Authors: Yousuf, Mohammad
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Visiting Friends and Relatives (VFR) travel is a significant form of travel in terms of global travel numbers. However, research on VFR travel is small relative to its size. In particular, research regarding the role of hosts of VFR travellers in shaping their trips including travel decisions and activities has been examined by few researchers. No previous research explored the differences in hosting between immigrant and non-immigrant local residents despite VFR travel being commonly associated with migration in existing literature. Before this research, the differences between hosting friends and relatives had been neglected, resulting in VFR hosts being treated as one homogenous group. Previous research also failed to empirically test the influence of destination on the hosting of VFRs. Thus, this is the first study examining the hosting of VFRs through combining how migration, relationship types, and destination types, impact VFR travel experiences for hosts. Given that VFR travel is a significant component of Australia’s visitor numbers, and that it comprises a large immigrant population, Australia is a suitable setting for this study. Considering the multi-dimensional elements in the study, the “VFR Whole Tourism Systems Model” was used as the conceptual model for this study. Quantitative research was conducted nationally with 331 residents, collected through an online survey, assessing the differences and similarities in hosting behaviours. Qualitative research was undertaken through in-depth interviews with 34 local residents in three contrasting destinations in Victoria understanding the social interactions between VFR hosts and their visiting friends/relatives. Significant differences were found between immigrants and nonimmigrants regarding attracting VFRs and hosting experiences. Differences were also noted between hosting friends versus hosting relatives, and it was also determined that the destination types impact VFR hosting. Such findings have provided valuable insights regarding the economic and social benefits of promoting local marketing campaign targeting local residents.
- Description: Doctor of Philosophy
- Authors: Yousuf, Mohammad
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Visiting Friends and Relatives (VFR) travel is a significant form of travel in terms of global travel numbers. However, research on VFR travel is small relative to its size. In particular, research regarding the role of hosts of VFR travellers in shaping their trips including travel decisions and activities has been examined by few researchers. No previous research explored the differences in hosting between immigrant and non-immigrant local residents despite VFR travel being commonly associated with migration in existing literature. Before this research, the differences between hosting friends and relatives had been neglected, resulting in VFR hosts being treated as one homogenous group. Previous research also failed to empirically test the influence of destination on the hosting of VFRs. Thus, this is the first study examining the hosting of VFRs through combining how migration, relationship types, and destination types, impact VFR travel experiences for hosts. Given that VFR travel is a significant component of Australia’s visitor numbers, and that it comprises a large immigrant population, Australia is a suitable setting for this study. Considering the multi-dimensional elements in the study, the “VFR Whole Tourism Systems Model” was used as the conceptual model for this study. Quantitative research was conducted nationally with 331 residents, collected through an online survey, assessing the differences and similarities in hosting behaviours. Qualitative research was undertaken through in-depth interviews with 34 local residents in three contrasting destinations in Victoria understanding the social interactions between VFR hosts and their visiting friends/relatives. Significant differences were found between immigrants and nonimmigrants regarding attracting VFRs and hosting experiences. Differences were also noted between hosting friends versus hosting relatives, and it was also determined that the destination types impact VFR hosting. Such findings have provided valuable insights regarding the economic and social benefits of promoting local marketing campaign targeting local residents.
- Description: Doctor of Philosophy
Voices from the margins : A critical ethnography of conflict in female friendship in a regional Australian school
- Authors: Pappaluca, Kimberly
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: The way that interpersonal conflict is displayed and navigated is informed by broader discourses about the nature of gender roles, gender expectations, and understandings of what it means to be a girl in regional Australia. This research explores the role of conflict in everyday school interactions for the female students of one regional secondary school in the state of Victoria, Australia. For these female students, the nature of their interpersonal conflict was either widely discussed and scrutinised by teachers, adults and other students, or ignored and silenced by the same groups. For the young women of Rural Valley, their experience of conflict is intrinsically tied to the cultural spaces and places they occupy. In this thesis, young women’s voices and experiences of conflict in a regional secondary school are considered through a critical perspective situated within critical theory. A critical ethnography has been conducted drawing upon the notion of horizontal violence to develop understandings of the nature of conflict as experienced by young women from regional Australia. In order to illuminate the lived experiences of conflict for young women, narrative portraiture is used as a representational method to deconstruct traditional views of ethnographic writing. In doing so, this research provides a counter-narrative to dominant discourses about how young women experience and manage conflict and how they navigate their relationships when conflict arises. This research is significant because it challenges stereotypical notions of what conflict means to young girls in a regional secondary school context. The findings of this study highlight that young women use group-specific strategies to negotiate friendships and confront structural inequalities of a hegemonic education system. This research ultimately advocates for understandings of conflict that move away from deficit discourses to advance discussions concerned with the gendered nature of violence within Australian society.
- Description: Doctor of Philosophy
- Authors: Pappaluca, Kimberly
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: The way that interpersonal conflict is displayed and navigated is informed by broader discourses about the nature of gender roles, gender expectations, and understandings of what it means to be a girl in regional Australia. This research explores the role of conflict in everyday school interactions for the female students of one regional secondary school in the state of Victoria, Australia. For these female students, the nature of their interpersonal conflict was either widely discussed and scrutinised by teachers, adults and other students, or ignored and silenced by the same groups. For the young women of Rural Valley, their experience of conflict is intrinsically tied to the cultural spaces and places they occupy. In this thesis, young women’s voices and experiences of conflict in a regional secondary school are considered through a critical perspective situated within critical theory. A critical ethnography has been conducted drawing upon the notion of horizontal violence to develop understandings of the nature of conflict as experienced by young women from regional Australia. In order to illuminate the lived experiences of conflict for young women, narrative portraiture is used as a representational method to deconstruct traditional views of ethnographic writing. In doing so, this research provides a counter-narrative to dominant discourses about how young women experience and manage conflict and how they navigate their relationships when conflict arises. This research is significant because it challenges stereotypical notions of what conflict means to young girls in a regional secondary school context. The findings of this study highlight that young women use group-specific strategies to negotiate friendships and confront structural inequalities of a hegemonic education system. This research ultimately advocates for understandings of conflict that move away from deficit discourses to advance discussions concerned with the gendered nature of violence within Australian society.
- Description: Doctor of Philosophy
Wild deer as potential reservoirs of economically important gastrointestinal parasites in South Eastern Australia
- Authors: Panozzo, Jacqueline
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Gastrointestinal (GI) parasite infection of ruminant livestock can be a source of significant economic loss for farmers. Appropriate animal management and drug treatments can reduce the transmission and subsequent effect of GI parasite infections on ruminant livestock productivity. However, transmission of infection from outside sources, such as wildlife, can make GI parasite control measures in livestock ineffective. In Australia, particularly Victoria, the large population of wild deer now represents a significant biosecurity risk due to the potential ability of economically important GI parasites of livestock to infect deer. This thesis aimed to investigate the presence of GI parasites in three species of Victorian wild deer (fallow, hog and sambar) by screening deer faecal samples through the traditional parasitology technique of faecal egg count (FEC) and through DNA-based molecular techniques of polymerase chain reaction (PCR) and next-generation sequencing (NGS). Screening wild deer faecal samples for strongyle parasites via FEC resulted in 15.1% positive samples. DNA based techniques were significantly more sensitive than FEC, with 60.0% deer faecal samples positive for strongyles. NGS detected DNA of eleven different strongyle species in wild deer faecal samples, including the economically important parasite species of livestock of O. ostertagi, O. leptospicularis, Haemonchus spp., O. radiatum, Trichostrongylus spp. and C. oncophora. Infection with the economically important trematode parasite of livestock, Fasciola hepatica, was also confirmed by worm recovery in hog deer. The same economically important strongyle species were also detected in faecal samples from wild deer co-grazing with cattle. Collectively, this study has indicated that wild deer in Victoria may be significant reservoirs of parasitic diseases of livestock and warrants further investigation.
- Description: Doctor of Philosophy
- Authors: Panozzo, Jacqueline
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Gastrointestinal (GI) parasite infection of ruminant livestock can be a source of significant economic loss for farmers. Appropriate animal management and drug treatments can reduce the transmission and subsequent effect of GI parasite infections on ruminant livestock productivity. However, transmission of infection from outside sources, such as wildlife, can make GI parasite control measures in livestock ineffective. In Australia, particularly Victoria, the large population of wild deer now represents a significant biosecurity risk due to the potential ability of economically important GI parasites of livestock to infect deer. This thesis aimed to investigate the presence of GI parasites in three species of Victorian wild deer (fallow, hog and sambar) by screening deer faecal samples through the traditional parasitology technique of faecal egg count (FEC) and through DNA-based molecular techniques of polymerase chain reaction (PCR) and next-generation sequencing (NGS). Screening wild deer faecal samples for strongyle parasites via FEC resulted in 15.1% positive samples. DNA based techniques were significantly more sensitive than FEC, with 60.0% deer faecal samples positive for strongyles. NGS detected DNA of eleven different strongyle species in wild deer faecal samples, including the economically important parasite species of livestock of O. ostertagi, O. leptospicularis, Haemonchus spp., O. radiatum, Trichostrongylus spp. and C. oncophora. Infection with the economically important trematode parasite of livestock, Fasciola hepatica, was also confirmed by worm recovery in hog deer. The same economically important strongyle species were also detected in faecal samples from wild deer co-grazing with cattle. Collectively, this study has indicated that wild deer in Victoria may be significant reservoirs of parasitic diseases of livestock and warrants further investigation.
- Description: Doctor of Philosophy
'You Beauty' Alex Jesaulenko An historical exploration of the migrant who became a legend
- Authors: Eddy, Daniel
- Date: 2019
- Type: Text , Thesis , PhD
- Full Text: false
- Description: The Austrian-born Alex Jesaulenko played football in the Victorian Football League (VFL) from 1967 to 1981. His rise to national prominence emerged during a period of great change within both Australian society and Australian rules football. This thesis, through a critical biographical approach, examines for the first time Jesaulenko’s early life, looking at his migrant experience and the role that Australian rules football played in aiding his integration into Australian society. It is not a kick-and-handball analysis of Jesaulenko’s entire football career; that has been extensively covered within copious amounts of primary and secondary sources. Instead, it explores his migrant journey – an important aspect of Jesaulenko’s life which has been largely overlooked – and the key developmental years of sporting education prior to emerging as a VFL champion. It concludes with one of, if not the most, iconic moments in the game’s history: Jesaulenko’s mark in the 1970 VFL grand final, which cemented his name within the Australian sporting consciousness. Australian rules football, and sport more generally, holds a unique place within society. Footballers, it can be argued, are archetypes for our daily dreams and aspirations; exalted figures that we afford status which few will experience in their everyday lives. Therefore, it is through the prism of Jesaulenko’s journey that we can learn more about the role Australian rules football has played for migrants integrating into Australian society.
- Description: Doctor of Philosophy