- Title
- Cloning, expression, purification and crystallographic studies of galectin-11 from domestic sheep (Ovis aries)
- Creator
- Sakthivel, Dhanasekaran; Littler, Dene; Shahine, Adam; Troy, Sally; Johnson, Matthew; Rossjohn, Jamie; Piedrafita, David; Beddoe, Travis
- Date
- 2015
- Type
- Text; Journal article
- Identifier
- http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/155399
- Identifier
- vital:11293
- Identifier
-
https://doi.org/10.1107/S2053230X15010195
- Identifier
- ISSN:2053-230X
- Abstract
- Galectins are an evolutionarily conserved family of proteins that translate glycan recognition into cellular effects. Galectin-11 is a unique member of the galectin family that is only expressed in ruminants such as sheep, goat and cattle and that plays a critical role in several important biological processes, such as reproduction and parasite-mediated innate immune responses. Currently, these two areas are of major importance for the sustainability of ruminant livestock production. Despite the emerging biological significance of galectin-11, no structural information is available. It is expected that structural studies will unravel the functional mechanisms of galectin-11 activity. Here, the expression, purification and crystallization of the ruminant-specific galectin-11 from domestic sheep and the collection of X-ray data to 2.0 Å resolution are reported. © 2015.
- Publisher
- International Union of Crystallography
- Relation
- Acta Crystallographica Section:F Structural Biology Communications Vol. 71, no. (2015), p. 993-997
- Rights
- Copyright © 2015 International Union of Crystallography
- Rights
- Open Access
- Rights
- This metadata is freely available under a CCO license
- Subject
- Galectin; Glycan; Livestock; Parasite; 03 Chemical Sciences; 06 Biological Sciences
- Full Text
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