- Title
- Validating the use of non-invasively sourced DNA for population genetic studies using pedigree data
- Creator
- Wedrowicz, Faye; Mosse, Jennifer; Wright, Wendy; Hogan, Fiona
- Date
- 2017
- Type
- Text; Journal article
- Identifier
- http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/155330
- Identifier
- vital:11307
- Identifier
-
https://doi.org/10.5194/we-17-9-2017
- Identifier
- ISSN:1399-1183
- Abstract
- Non-invasive genetic sampling has provided valuable ecological data for many species - data which may have been unobtainable using invasive sampling methods. However, DNA obtained non-invasively may be prone to increased levels of amplification failure and genotyping error. Utilizing genotype data from 32 pedigreed koalas, this study aimed to validate the reliability of final consensus genotypes obtained using DNA isolated from koala scats. Pedigree analysis, duplicate genotyping, analysis of mismatched loci and tests for null alleles were used to look for evidence of errors. All genetically confirmed parent-offspring relationships were found to follow Mendelian rules of inheritance. Duplicate genotypes matched in all cases and there was no evidence of null alleles. Related individuals always had different 12-marker genotypes having a minimum of three unique loci (in one full sibling pair), a mode of seven unique loci and a maximum of 11 unique loci. This study demonstrates the capacity of DNA recovered from koala scats to provide reliable genotypes that can unequivocally discriminate individuals and infer parentage, provided data are missing from no more than two loci. Validating data obtained using non-invasive sampling is an important step, allowing potential problems to be identified at an early stage. © Author(s) 2017. CC Attribution 3.0 License.
- Publisher
- Copernicus GmbH
- Relation
- Web Ecology Vol. 17, no. 1 (2017), p. 9-18
- Rights
- Copyright © Author(s) 2017. CC Attribution 3.0 License (http://creativecommons.org/licenses/by/3.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- Rights
- Open Access
- Rights
- This metadata is freely available under a CCO license
- Subject
- Phascolarctidae; DNA; Population genetics; Ecological data; Validating data
- Full Text
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