Evaluation of colorimetric detection methods for Shigella, Salmonella, and Vibrio cholerae by loop-mediated isothermal amplification

Title: Evaluation of colorimetric detection methods for Shigella, Salmonella, and Vibrio cholerae by loop-mediated isothermal amplification
Creator: Soli, Kevin; Kas, Monalisa; Maure, Tobias; Umezaki, Masahiro; Morita, Ayako; Siba, Peter; Greenhill, Andrew; Horwood, Paul
Date: 2013
Type: Text; Journal article
Identifier: http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/70532
Identifier: vital:6527
Identifier: https://doi.org/10.1016/j.diagmicrobio.2013.09.009
Identifier: ISSN:0732-8893
Abstract: We evaluated loop-mediated isothermal amplification end-point detection methods for Salmonella, Shigella, and Vibrio cholerae. Detection sensitivities were comparable to real-time PCR methods. The colorimetric dyes hydroxynaphthol blue and SYBR Green I showed increased sensitivity when compared to visual and automated turbidity readings. End-point colorimetric dyes promise great utility in developing settings.
Relation: Diagnostic Microbiology and Infectious Disease Vol. 77, no. 4 (2013), p. 321-323
Rights: Copyright Elsevier
Rights: This metadata is freely available under a CCO license
Subject: 0605 Microbiology; 1108 Medical Microbiology; LAMP; Loop-mediated isothermal amplification; Colorimetric detection; Hydroxynaphthol blue; SYBR Green; Salmonella; Shigella; Vibrio cholerae
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