Epigenetic modifications in essential hypertension
- Authors: Wise, Ingrid
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Background: Hypertension (HTN) is a complex, multifactorial, quantitative trait under polygenic control that affects more than one billion people globally. Despite advances in our understanding of the pathophysiology of HTN and the implementation of more effective treatment and prevention strategies, HTN remains one of the world’s great public health problems. The accepted inference from genome-wide association studies (GWAS) is that the genetic code lays the foundation for transcriptomic changes and in turn physiological change. On the other side of the coin, environmental factors (smoking, diet, chemical exposure) can in turn affect DNA itself in genes relevant to blood pressure (BP). Variation in epigenetic forms of modification may thus explain additional phenotypic variation in BP and provide new clues to the physiological processes influencing its regulation. DNA methylation is one of these epigenetic mechanisms responsible for changes to gene expression, activated by interaction with environmental triggers. DNA methylation is a reversible epigenetic modifier of specific dinucleotide sites called CpGs, which consists of a transfer of a methyl group derived from S-adenosyl-L-methionine to position five of a cytosine ring, forming 5mC. Pathophysiologically, the kidney is known as the key organ of BP regulation and one of the most important contributors to HTN. According to the hypothesis put forward by Guyton, over 40 years ago, the control of BP in the steady-state and longer-term is critically dependent on renal mechanisms. In fact, almost all monogenic forms of HTN are driven by rare mutations in genes involved in salt handling in the distal nephron. It is therefore crucial to understand kidney DNA methylation changes that may drive gene expression in kidney and lead to HTN. Hypothesis: The central hypothesis underpinning this PhD thesis is that alterations in kidney specific DNA methylation plays a fundamental role in modulating gene expression changes involved in the regulation of BP and pathophysiology of EH. Aims: This PhD thesis focuses on characterising the role of DNA methylation in the hypertensive kidney using array and RNA-sequencing methods. Three major aims are addressed: • Aim 1: To characterise blood and kidney global DNA methylation dynamics and its functional role in the hypertensive population (Chapter 3). • Aim 2: To determine the role of genome-wide, loci specific DNA methylation in the hypertensive human kidney (Chapter 4). • Aim 3: To understand the relationship between DNA methylation and differential expression of genes associated with BP and HTN in the human kidney (Chapter 5). Results: In Aim 1 global DNA methylation changes were characterised in peripheral blood leukocyte and kidney DNA of the hypertensive (HT) population using he ELISA method. We found no association between HTN diagnosis and global methylation percentage in either peripheral blood leukocytes or kidney DNA. However, a negative correlation was found between global methylation and diastolic blood pressure (DBP), yet this relationship was not evident after adjustment for the effect of antihypertensive medication. Furthermore, we investigated the sensitivity of ELISA-based global methylation detection by calculating the percentage of global methylation in kidney using array based methods; the results were similar, demonstrating no association between HTN diagnosis and median kidney methylation
- Description: Doctor of Philosophy
- Authors: Wise, Ingrid
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Background: Hypertension (HTN) is a complex, multifactorial, quantitative trait under polygenic control that affects more than one billion people globally. Despite advances in our understanding of the pathophysiology of HTN and the implementation of more effective treatment and prevention strategies, HTN remains one of the world’s great public health problems. The accepted inference from genome-wide association studies (GWAS) is that the genetic code lays the foundation for transcriptomic changes and in turn physiological change. On the other side of the coin, environmental factors (smoking, diet, chemical exposure) can in turn affect DNA itself in genes relevant to blood pressure (BP). Variation in epigenetic forms of modification may thus explain additional phenotypic variation in BP and provide new clues to the physiological processes influencing its regulation. DNA methylation is one of these epigenetic mechanisms responsible for changes to gene expression, activated by interaction with environmental triggers. DNA methylation is a reversible epigenetic modifier of specific dinucleotide sites called CpGs, which consists of a transfer of a methyl group derived from S-adenosyl-L-methionine to position five of a cytosine ring, forming 5mC. Pathophysiologically, the kidney is known as the key organ of BP regulation and one of the most important contributors to HTN. According to the hypothesis put forward by Guyton, over 40 years ago, the control of BP in the steady-state and longer-term is critically dependent on renal mechanisms. In fact, almost all monogenic forms of HTN are driven by rare mutations in genes involved in salt handling in the distal nephron. It is therefore crucial to understand kidney DNA methylation changes that may drive gene expression in kidney and lead to HTN. Hypothesis: The central hypothesis underpinning this PhD thesis is that alterations in kidney specific DNA methylation plays a fundamental role in modulating gene expression changes involved in the regulation of BP and pathophysiology of EH. Aims: This PhD thesis focuses on characterising the role of DNA methylation in the hypertensive kidney using array and RNA-sequencing methods. Three major aims are addressed: • Aim 1: To characterise blood and kidney global DNA methylation dynamics and its functional role in the hypertensive population (Chapter 3). • Aim 2: To determine the role of genome-wide, loci specific DNA methylation in the hypertensive human kidney (Chapter 4). • Aim 3: To understand the relationship between DNA methylation and differential expression of genes associated with BP and HTN in the human kidney (Chapter 5). Results: In Aim 1 global DNA methylation changes were characterised in peripheral blood leukocyte and kidney DNA of the hypertensive (HT) population using he ELISA method. We found no association between HTN diagnosis and global methylation percentage in either peripheral blood leukocytes or kidney DNA. However, a negative correlation was found between global methylation and diastolic blood pressure (DBP), yet this relationship was not evident after adjustment for the effect of antihypertensive medication. Furthermore, we investigated the sensitivity of ELISA-based global methylation detection by calculating the percentage of global methylation in kidney using array based methods; the results were similar, demonstrating no association between HTN diagnosis and median kidney methylation
- Description: Doctor of Philosophy
First World War Avenues of Honour : Social history through the landscape
- Authors: Taffe, Michael
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: This thesis argues that avenues of honour were the first choice of memorial to the Great War created by Australians. Despite not being the first such avenue, the thesis argues that, by virtue of the massive amount of publicity it brought to focus on this form of memorial, the Ballarat Avenue of Honour was a significant cultural statement by Australians during the Great War. The Ballarat Avenue of Honour was inspirational and pivotal to the establishment of a movement that saw similar memorial avenues planted throughout Australia and also in the U.S.A., U.K., Canada and New Zealand. Using examples from municipal council minutes, correspondence and newspaper reports the spread of this form of memorial is followed from its infancy in South Australia through the Ballarat experience to Britain, North America and New Zealand. Following Australia‘s first plantings in 1915, there was a groundswell from many communities throughout Australia who adopted this form of memorialisation. Australian communities took control of their own need to honour their heroes, their local volunteers, in avenue of honour plantings. Following the example of Ballarat after 1917, this desire to plant memorial avenues became a movement. Examples of the growth of this memorial movement, while government aimed to control spending by curtailing ‗waste‘ on memorials, are outlined and analysed. The thesis also examines the symbolism of avenues against the perceived superior ‗worthiness‘ of later built memorials. By the time the movement declined in Australia, other countries were continued to plant avenues. The diminution, and eventual fall, from memory of many of these heritage landscapes is explored as a part of the politics of identity. In examining the arguments, the links between Ballarat‘s avenue and others throughout Australia, the respective Commonwealth countries as well as the U.S.A. are developed.
- Description: Doctor of Philosophy
- Authors: Taffe, Michael
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: This thesis argues that avenues of honour were the first choice of memorial to the Great War created by Australians. Despite not being the first such avenue, the thesis argues that, by virtue of the massive amount of publicity it brought to focus on this form of memorial, the Ballarat Avenue of Honour was a significant cultural statement by Australians during the Great War. The Ballarat Avenue of Honour was inspirational and pivotal to the establishment of a movement that saw similar memorial avenues planted throughout Australia and also in the U.S.A., U.K., Canada and New Zealand. Using examples from municipal council minutes, correspondence and newspaper reports the spread of this form of memorial is followed from its infancy in South Australia through the Ballarat experience to Britain, North America and New Zealand. Following Australia‘s first plantings in 1915, there was a groundswell from many communities throughout Australia who adopted this form of memorialisation. Australian communities took control of their own need to honour their heroes, their local volunteers, in avenue of honour plantings. Following the example of Ballarat after 1917, this desire to plant memorial avenues became a movement. Examples of the growth of this memorial movement, while government aimed to control spending by curtailing ‗waste‘ on memorials, are outlined and analysed. The thesis also examines the symbolism of avenues against the perceived superior ‗worthiness‘ of later built memorials. By the time the movement declined in Australia, other countries were continued to plant avenues. The diminution, and eventual fall, from memory of many of these heritage landscapes is explored as a part of the politics of identity. In examining the arguments, the links between Ballarat‘s avenue and others throughout Australia, the respective Commonwealth countries as well as the U.S.A. are developed.
- Description: Doctor of Philosophy
From gold field to municipality : The establishment of Ballarat West 1855-1857
- Authors: Cartledge, Graeme
- Date: 2018
- Type: Text , Thesis , Masters
- Full Text:
- Description: This thesis examines the establishment of the Ballarat West Municipality in the years of 1855 – 1857 and the factors that contributed to the introduction of local self-government in the immediate aftermath of the Eureka Stockade. Underlying the study is the changing administrative requirements necessitated by the transition from a temporary gold field to a permanent city. A central theme explored in relation to this development is that it was a consequence of the emerging culture of modernity of that era precipitating radical political changes in local government that began with the 1835 British Municipal Corporations Act. This theme is expanded to highlight the reform of local government in the Victorian era in response to urbanization and the need for modern and rationalised methods of managing the new towns and growing cities. The difficulty in making and sustaining such progressive changes in Britain is contrasted with the eager adoption of the concept of progress and the new Victorian Municipal Corporations Act of 1854 on the Ballarat goldfields. The question as to why the Municipality was established is answered by exploring the connection between the failure of the Goldfields Commission at the end of 1854 and the belief held by many, that taxes should be accompanied with political representation and should be spent where they were collected. This study exposes the remarkable story of how the first elected councillors, starting from scratch, quickly established administrative systems and brought order to a community emerging out of turmoil. The process of how the municipality was established is uncovered by an extensive survey of the council minutes, the media, council correspondence and public records.
- Description: Masters by Research
- Authors: Cartledge, Graeme
- Date: 2018
- Type: Text , Thesis , Masters
- Full Text:
- Description: This thesis examines the establishment of the Ballarat West Municipality in the years of 1855 – 1857 and the factors that contributed to the introduction of local self-government in the immediate aftermath of the Eureka Stockade. Underlying the study is the changing administrative requirements necessitated by the transition from a temporary gold field to a permanent city. A central theme explored in relation to this development is that it was a consequence of the emerging culture of modernity of that era precipitating radical political changes in local government that began with the 1835 British Municipal Corporations Act. This theme is expanded to highlight the reform of local government in the Victorian era in response to urbanization and the need for modern and rationalised methods of managing the new towns and growing cities. The difficulty in making and sustaining such progressive changes in Britain is contrasted with the eager adoption of the concept of progress and the new Victorian Municipal Corporations Act of 1854 on the Ballarat goldfields. The question as to why the Municipality was established is answered by exploring the connection between the failure of the Goldfields Commission at the end of 1854 and the belief held by many, that taxes should be accompanied with political representation and should be spent where they were collected. This study exposes the remarkable story of how the first elected councillors, starting from scratch, quickly established administrative systems and brought order to a community emerging out of turmoil. The process of how the municipality was established is uncovered by an extensive survey of the council minutes, the media, council correspondence and public records.
- Description: Masters by Research
Gas chromatographic-mass spectrometry analysis of volatile organic compounds from cancer cell cultures - The effect of hypoxia
- Authors: Kalluri, Usha
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Early diagnosis of lung cancer improves patient outcomes which has led to a search for non-invasive diagnostic tests suitable for population screening. Volatile organic compounds (VOCs) in exhaled breath have shown potential, however, confirmation of the metabolic origins and disease specificity of candidate markers is required. Cell culture metabolomics can identify disease biomarkers and their origins. To date VOC profiles from in vitro cultured cancer cells have little similarity to cancer breath VOC profiles. In vivo, cancer cells experience hypoxia whereas in vitro cells are cultured under normoxic conditions. Since hypoxia influences cell metabolism, we hypothesize that cancer cells cultured under hypoxic conditions will have altered cell metabolism and produce VOC profiles more typical of cancer breathe. This study investigates the effect of hypoxia on metabolic reprogramming in A549 lung cancer cells cultured under standard normoxic (atmospheric oxygen) or hypoxic (2% oxygen) conditions. Results from quantitative RT-PCR demonstrated a significant upregulation in hypoxia of the glucose transporter (GLUT1) and the key TCA regulatory gene PDHK1, demonstrating that hypoxia plays a pivotal role in regulating metabolism in A549 cells. A ratio-metric assessment of Lipid Peroxidation (LPO) and the production of reactive oxygen species (ROS) showed an increase in LPO and a slight decrease in the production of ROS in hypoxic cultures, the combined effect of which may serve to equip the cells to adapt to and proliferate under low oxygen. Finally, the comparison of endogenous VOCs produced by A549 cells under hypoxic and normoxic conditions identified twelve VOCs unique to cells grown under hypoxic conditions including n-pentane, a marker of LPO and cancer, and 3-methyl hexane, which has been reported as a biomarker of cancer. This data is consistent with the hypothesis that a hypoxic tumour microenvironment may influence cell metabolism leading to a unique and diagnostic cancer VOC profile.
- Description: Doctor of Philosophy
- Authors: Kalluri, Usha
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Early diagnosis of lung cancer improves patient outcomes which has led to a search for non-invasive diagnostic tests suitable for population screening. Volatile organic compounds (VOCs) in exhaled breath have shown potential, however, confirmation of the metabolic origins and disease specificity of candidate markers is required. Cell culture metabolomics can identify disease biomarkers and their origins. To date VOC profiles from in vitro cultured cancer cells have little similarity to cancer breath VOC profiles. In vivo, cancer cells experience hypoxia whereas in vitro cells are cultured under normoxic conditions. Since hypoxia influences cell metabolism, we hypothesize that cancer cells cultured under hypoxic conditions will have altered cell metabolism and produce VOC profiles more typical of cancer breathe. This study investigates the effect of hypoxia on metabolic reprogramming in A549 lung cancer cells cultured under standard normoxic (atmospheric oxygen) or hypoxic (2% oxygen) conditions. Results from quantitative RT-PCR demonstrated a significant upregulation in hypoxia of the glucose transporter (GLUT1) and the key TCA regulatory gene PDHK1, demonstrating that hypoxia plays a pivotal role in regulating metabolism in A549 cells. A ratio-metric assessment of Lipid Peroxidation (LPO) and the production of reactive oxygen species (ROS) showed an increase in LPO and a slight decrease in the production of ROS in hypoxic cultures, the combined effect of which may serve to equip the cells to adapt to and proliferate under low oxygen. Finally, the comparison of endogenous VOCs produced by A549 cells under hypoxic and normoxic conditions identified twelve VOCs unique to cells grown under hypoxic conditions including n-pentane, a marker of LPO and cancer, and 3-methyl hexane, which has been reported as a biomarker of cancer. This data is consistent with the hypothesis that a hypoxic tumour microenvironment may influence cell metabolism leading to a unique and diagnostic cancer VOC profile.
- Description: Doctor of Philosophy
Green bonds : Do they bring sustainability to environmentally friendly projects?
- Authors: Nanayakkara, Madurika
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: This study was motivated by the urgent requirement to scale up the global green bond (GB) market to meet the trillion-dollar financial requirement of environmental resilience projects worldwide, and by the absence of prior scientific investigations to determine solutions to the GB market’s problems from different perspectives. Three empirical studies were conducted to provide a holistic view of the GB market. The first study investigated the perception gaps of different stakeholders in the GB market in terms of assessing the ‘greenness’ of projects funded through GBs, investors’ expectations of GB investments, and the factors hindering the growth of the GB market. A qualitative method was employed with document review, followed by interviews, a questionnaire survey and peer debriefing. The study found that both the demand and supply sides of the GB market are inadequate to meet the world’s green investment requirements. The main obstacles hindering the market growth are fear for greenwashing, definitions that lack clarity, the absence of large-scale issues, and the high costs associated with complex reporting processes and third-party certifications. The second study assessed the influence of GB principles on investor demand for GBs, using global GBs issued for the period 2007 to 2016. Bid–ask spread and yield spread were used to measure the investor demand. The results indicated a significant positive relationship between the degree of compliance and investor demand. The third study compared the credit spreads of corporate GBs and conventional bonds, measured by option-adjusted spread daily data for the period 2016 to 2017 worldwide. Hybrid method of panel data regression was employed to analyse the data, and found that GBs are traded at a premium in the world market, compared with conventional bonds. This thesis suggests several policy implications based on the findings of these studies to scale up the GB market as a new source of financing.
- Description: Doctor of Philosophy
- Authors: Nanayakkara, Madurika
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: This study was motivated by the urgent requirement to scale up the global green bond (GB) market to meet the trillion-dollar financial requirement of environmental resilience projects worldwide, and by the absence of prior scientific investigations to determine solutions to the GB market’s problems from different perspectives. Three empirical studies were conducted to provide a holistic view of the GB market. The first study investigated the perception gaps of different stakeholders in the GB market in terms of assessing the ‘greenness’ of projects funded through GBs, investors’ expectations of GB investments, and the factors hindering the growth of the GB market. A qualitative method was employed with document review, followed by interviews, a questionnaire survey and peer debriefing. The study found that both the demand and supply sides of the GB market are inadequate to meet the world’s green investment requirements. The main obstacles hindering the market growth are fear for greenwashing, definitions that lack clarity, the absence of large-scale issues, and the high costs associated with complex reporting processes and third-party certifications. The second study assessed the influence of GB principles on investor demand for GBs, using global GBs issued for the period 2007 to 2016. Bid–ask spread and yield spread were used to measure the investor demand. The results indicated a significant positive relationship between the degree of compliance and investor demand. The third study compared the credit spreads of corporate GBs and conventional bonds, measured by option-adjusted spread daily data for the period 2016 to 2017 worldwide. Hybrid method of panel data regression was employed to analyse the data, and found that GBs are traded at a premium in the world market, compared with conventional bonds. This thesis suggests several policy implications based on the findings of these studies to scale up the GB market as a new source of financing.
- Description: Doctor of Philosophy
Investigating bird responses to fire in the Heathy Dry Forests of Victoria, Australia
- Authors: Kuchinke, Diana
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Globally, forest birds are under pressure, from agriculture and urban development fragmenting the landscape. Adding to these pressures, changes in the patterns of global climate drivers give rise to an increase in the frequency of extreme weather events. In Victoria, Australia, changing weather conditions are resulting in increases in the frequency and extent of bushfires. Furthermore, prescribed burning is applied to the landscape in attempts to not only ameliorate the impacts from bushfire, but as part of a ‘pyrodiversity begets biodiversity’ protocol. These pressures all impact birds by reducing resources for: food, nesting and protection from predators. This thesis investigated bird responses to fire in the Heathy Dry Forests of Victoria, against variables of time-since-fire and fire frequency. Bird responses were modelled in terms of: community, foraging guilds and individual species. As a community, birds showed a resilience to both time since fire and fire frequency. Responses by foraging guilds and individual species highlighted some different responses. The common species from each foraging guild showed responses that broadly represent their guild. This thesis highlighted that an effective tool in adaptive management is to predict the trends of our common forest birds, as surrogates for entire bird communities, not just for fire responses, but for a broader reflection on the health of the landscape. The modelling of one species, the Laughing Kookaburra, showed a response to both time since fire and fire frequency, with a reduced abundance in post-fire new-growth vegetation. As this species is noted as being in decline down the east coast of Australia, it is flagged in this thesis as a species of concern. Further, this thesis investigated alpha and beta responses by the forest birds to prescribed burns of different severities. Results suggest that forest birds show little response to small prescribed burns in the landscape, regardless of severity. This may be a result of Heathy Dry Forests’ rapid regeneration post-fire. However, one species that exhibits site fidelity, the White-throated Treecreeper, left areas impacted by high severity prescribed burns. The White-throated Treecreeper’s response flagged the importance of tree hollows being maintained in the landscape, essential for the species that require hollows for roosting and nesting.
- Description: Doctor of Philosophy
- Description: Globally, forest birds are under pressure, from agriculture and urban development fragmenting the landscape. Adding to these pressures, changes in the patterns of global climate drivers give rise to an increase in the frequency of extreme weather events. In Victoria, Australia, changing weather conditions are resulting in increases in the frequency and extent of bushfires. Furthermore, prescribed burning is applied to the landscape in attempts to not only ameliorate the impacts from bushfire, but as part of a ‘pyrodiversity begets biodiversity’ protocol. These pressures all impact birds by reducing resources for: food, nesting and protection from predators. This thesis investigated bird responses to fire in the Heathy Dry Forests of Victoria, against variables of time-since-fire and fire frequency. Bird responses were modelled in terms of: community, foraging guilds and individual species. As a community, birds showed a resilience to both time since fire and fire frequency. Responses by foraging guilds and individual species highlighted some different responses. The common species from each foraging guild showed responses that broadly represent their guild. This thesis highlighted that an effective tool in adaptive management is to predict the trends of our common forest birds, as surrogates for entire bird communities, not just for fire responses, but for a broader reflection on the health of the landscape. The modelling of one species, the Laughing Kookaburra, showed a response to both time since fire and fire frequency, with a reduced abundance in post-fire new-growth vegetation. As this species is noted as being in decline down the east coast of Australia, it is flagged in this thesis as a species of concern. Further, this thesis investigated alpha and beta responses by the forest birds to prescribed burns of different severities. Results suggest that forest birds show little response to small rescribed burns in the landscape, regardless of severity. This may be a result of Heathy Dry Forests’ rapid regeneration post-fire. However, one species that exhibits site fidelity, the White-throated Treecreeper, left areas impacted by high severity prescribed burns. The White-throated Treecreeper’s response flagged the importance of tree hollows being maintained in the landscape, essential for the species that require hollows for roosting and nesting.
- Authors: Kuchinke, Diana
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Globally, forest birds are under pressure, from agriculture and urban development fragmenting the landscape. Adding to these pressures, changes in the patterns of global climate drivers give rise to an increase in the frequency of extreme weather events. In Victoria, Australia, changing weather conditions are resulting in increases in the frequency and extent of bushfires. Furthermore, prescribed burning is applied to the landscape in attempts to not only ameliorate the impacts from bushfire, but as part of a ‘pyrodiversity begets biodiversity’ protocol. These pressures all impact birds by reducing resources for: food, nesting and protection from predators. This thesis investigated bird responses to fire in the Heathy Dry Forests of Victoria, against variables of time-since-fire and fire frequency. Bird responses were modelled in terms of: community, foraging guilds and individual species. As a community, birds showed a resilience to both time since fire and fire frequency. Responses by foraging guilds and individual species highlighted some different responses. The common species from each foraging guild showed responses that broadly represent their guild. This thesis highlighted that an effective tool in adaptive management is to predict the trends of our common forest birds, as surrogates for entire bird communities, not just for fire responses, but for a broader reflection on the health of the landscape. The modelling of one species, the Laughing Kookaburra, showed a response to both time since fire and fire frequency, with a reduced abundance in post-fire new-growth vegetation. As this species is noted as being in decline down the east coast of Australia, it is flagged in this thesis as a species of concern. Further, this thesis investigated alpha and beta responses by the forest birds to prescribed burns of different severities. Results suggest that forest birds show little response to small prescribed burns in the landscape, regardless of severity. This may be a result of Heathy Dry Forests’ rapid regeneration post-fire. However, one species that exhibits site fidelity, the White-throated Treecreeper, left areas impacted by high severity prescribed burns. The White-throated Treecreeper’s response flagged the importance of tree hollows being maintained in the landscape, essential for the species that require hollows for roosting and nesting.
- Description: Doctor of Philosophy
- Description: Globally, forest birds are under pressure, from agriculture and urban development fragmenting the landscape. Adding to these pressures, changes in the patterns of global climate drivers give rise to an increase in the frequency of extreme weather events. In Victoria, Australia, changing weather conditions are resulting in increases in the frequency and extent of bushfires. Furthermore, prescribed burning is applied to the landscape in attempts to not only ameliorate the impacts from bushfire, but as part of a ‘pyrodiversity begets biodiversity’ protocol. These pressures all impact birds by reducing resources for: food, nesting and protection from predators. This thesis investigated bird responses to fire in the Heathy Dry Forests of Victoria, against variables of time-since-fire and fire frequency. Bird responses were modelled in terms of: community, foraging guilds and individual species. As a community, birds showed a resilience to both time since fire and fire frequency. Responses by foraging guilds and individual species highlighted some different responses. The common species from each foraging guild showed responses that broadly represent their guild. This thesis highlighted that an effective tool in adaptive management is to predict the trends of our common forest birds, as surrogates for entire bird communities, not just for fire responses, but for a broader reflection on the health of the landscape. The modelling of one species, the Laughing Kookaburra, showed a response to both time since fire and fire frequency, with a reduced abundance in post-fire new-growth vegetation. As this species is noted as being in decline down the east coast of Australia, it is flagged in this thesis as a species of concern. Further, this thesis investigated alpha and beta responses by the forest birds to prescribed burns of different severities. Results suggest that forest birds show little response to small rescribed burns in the landscape, regardless of severity. This may be a result of Heathy Dry Forests’ rapid regeneration post-fire. However, one species that exhibits site fidelity, the White-throated Treecreeper, left areas impacted by high severity prescribed burns. The White-throated Treecreeper’s response flagged the importance of tree hollows being maintained in the landscape, essential for the species that require hollows for roosting and nesting.
Investigating the impact of participation in alternative education from a positive youth development perspective : A case study of the School for Student Leadership
- Authors: Joyce, Susan
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: The School for Student Leadership (SSL) has existed since 2001 as an alternative residential educational setting for Year 9 students in Victoria, Australia. It was designed in response to growing calls for more effective ways of engaging middle school students. A review of the literature illustrated a dearth of research regarding alternative programs and schools, particularly from a positive youth development (PYD) perspective, and relating to long term effects of participation. This study investigated student participants’ perceived development in relation to the Five Cs - Confidence, Competence, Character, Connection and Care, concepts derived from the framework of PYD. It also examined differences in perceptions of students attending a shorter five-week program, compared to the standard nine-week program, as well as differences between male and female participants. This case study was undertaken utilising a mixed methods approach, using pre and post program surveys and interviews. Between 2013 and 2014, 385 students were surveyed and 58 interviews conducted over nine program offerings. The participants were then followed up one year post program, with 172 surveys returned and 14 interviews conducted. With the assistance of computer software programs, both the quantitative and qualitative data was analysed and then triangulated to form the findings. Participants’ ratings for each of the Five Cs were higher at the end of the program, and effect sizes, though generally small, when combined with the qualitative data indicated the program as having a significant positive impact, although this diminished slightly one year post program. Little discernible difference was found between the five- and the nine-week programs, and only slight differences between the two genders. The set of recommendations that emanated from this research should be of assistance for both the SSL and other schools, particularly in relation to enhancing attributes such as the Five Cs.
- Description: Doctor of Philosophy
- Authors: Joyce, Susan
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: The School for Student Leadership (SSL) has existed since 2001 as an alternative residential educational setting for Year 9 students in Victoria, Australia. It was designed in response to growing calls for more effective ways of engaging middle school students. A review of the literature illustrated a dearth of research regarding alternative programs and schools, particularly from a positive youth development (PYD) perspective, and relating to long term effects of participation. This study investigated student participants’ perceived development in relation to the Five Cs - Confidence, Competence, Character, Connection and Care, concepts derived from the framework of PYD. It also examined differences in perceptions of students attending a shorter five-week program, compared to the standard nine-week program, as well as differences between male and female participants. This case study was undertaken utilising a mixed methods approach, using pre and post program surveys and interviews. Between 2013 and 2014, 385 students were surveyed and 58 interviews conducted over nine program offerings. The participants were then followed up one year post program, with 172 surveys returned and 14 interviews conducted. With the assistance of computer software programs, both the quantitative and qualitative data was analysed and then triangulated to form the findings. Participants’ ratings for each of the Five Cs were higher at the end of the program, and effect sizes, though generally small, when combined with the qualitative data indicated the program as having a significant positive impact, although this diminished slightly one year post program. Little discernible difference was found between the five- and the nine-week programs, and only slight differences between the two genders. The set of recommendations that emanated from this research should be of assistance for both the SSL and other schools, particularly in relation to enhancing attributes such as the Five Cs.
- Description: Doctor of Philosophy
- Authors: Ranjbar Pouya, Kaveh
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text: false
- Description: Coode Island Silt (CIS) is one of the predominant geological units in Melbourne, Australia. Having high compressibility and low shear strength, CIS is considered a problematic soft soil that challenges the construction of infrastructure in the region. To tackle such challenges, one practical approach is the application of ground improvement techniques such as in situ soil mixing. This PhD study focuses on the application of Cutter Soil Mixing (CSM) for the construction of excavation retaining walls in CIS. Although cement is widely used in most CSM projects, this study investigates the suitability of different lime types available in the Australian market as a potential alternative to cement for the stabilisation of CIS. To investigate the effect of lime stabilisation, a comprehensive geotechnical characterisation of untreated and lime treated CIS is performed. Four different lime types are used: agricultural lime, quicklime, hydrated lime and slag lime. Based on the results obtained from strength tests, slag lime was found to be the most effective among the four types that were tested. The optimum slag lime to CIS ratio is then found for the construction of retaining walls in CIS. Having the geotechnical characterisation of untreated and treated CIS from the laboratory experiments, a series of two-dimensional and three-dimensional finite element method (FEM) analyses were conducted to investigate the applicability and reliability of the selected mixing ratio for the construction of CSM excavation retaining walls in CIS. A nonlinear constitutive soil model was employed, calibrated and verified to be used in FEM analyses to investigate both the stability factor of safety and excavation-induced deformations. The results obtained for both undrained and fully coupled flow deformation analyses prove that CSM panels can be constructed by mixing slag lime and CIS to act as retaining walls to allow for deep excavation in CIS.
- Description: Doctor of Philosophy
Measuring depression in deaf adults : Adaptation and validation of the Patient Health Questionnaire 9 (PHQ-9) for Auslan users
- Authors: Lake, Shane
- Date: 2018
- Type: Text , Thesis , Masters
- Full Text:
- Description: There is limited research into the prevalence of depression in the deaf population. Results are inconsistent and research has been hampered by the use of measures not specifically designed for individuals who are deaf. Deaf adults, who use Australian sign language (Auslan) to communicate, may not have the spoken and written language skills in English that are required to understand standard psychological measures. The aim of this research was to adapt and validate a measure of depression, the 9 item Patient Health Questionnaire (PHQ-9), for deaf Auslan users. Following established guidelines, an Auslan version of the PHQ-9, that was conceptually equivalent to the original measure, was produced. A community sample of 34 deaf adults, who use Auslan to communicate, and 278 hearing adults, were recruited from the Australian population. Deaf participants completed an Auslan online survey that included the Auslan version of the PHQ-9 and a previously adapted measure, an Auslan version of the Depression Anxiety Stress Scales-21 (DASS-21-Auslan). Hearing participants completed a written English version of the online survey. The Auslan version of the PHQ-9 demonstrated good internal reliability. Concurrent validity was established using the DASS-21-Auslan, with significant correlations found between the two measures. Principal components analysis identified a single factor structure for the Auslan PHQ-9. Differential item functioning was examined using a parametric technique (ordinal logistic regression) and a non-parametric kernel smoothing technique (TestGraf). No evidence of item bias was found. This research has provided promising results for an Auslan version of the PHQ-9 as a culturally appropriate measure for Auslan users. The Auslan PHQ-9 has the potential to provide mental health practitioners and researchers with a more accurate method of assessing and monitoring depression and depressive symptoms in deaf adults who are Auslan users.
- Description: Masters by Research
- Authors: Lake, Shane
- Date: 2018
- Type: Text , Thesis , Masters
- Full Text:
- Description: There is limited research into the prevalence of depression in the deaf population. Results are inconsistent and research has been hampered by the use of measures not specifically designed for individuals who are deaf. Deaf adults, who use Australian sign language (Auslan) to communicate, may not have the spoken and written language skills in English that are required to understand standard psychological measures. The aim of this research was to adapt and validate a measure of depression, the 9 item Patient Health Questionnaire (PHQ-9), for deaf Auslan users. Following established guidelines, an Auslan version of the PHQ-9, that was conceptually equivalent to the original measure, was produced. A community sample of 34 deaf adults, who use Auslan to communicate, and 278 hearing adults, were recruited from the Australian population. Deaf participants completed an Auslan online survey that included the Auslan version of the PHQ-9 and a previously adapted measure, an Auslan version of the Depression Anxiety Stress Scales-21 (DASS-21-Auslan). Hearing participants completed a written English version of the online survey. The Auslan version of the PHQ-9 demonstrated good internal reliability. Concurrent validity was established using the DASS-21-Auslan, with significant correlations found between the two measures. Principal components analysis identified a single factor structure for the Auslan PHQ-9. Differential item functioning was examined using a parametric technique (ordinal logistic regression) and a non-parametric kernel smoothing technique (TestGraf). No evidence of item bias was found. This research has provided promising results for an Auslan version of the PHQ-9 as a culturally appropriate measure for Auslan users. The Auslan PHQ-9 has the potential to provide mental health practitioners and researchers with a more accurate method of assessing and monitoring depression and depressive symptoms in deaf adults who are Auslan users.
- Description: Masters by Research
Negotiating policy - renegotiating practice : Understanding language, literacy and numeracy teachers' professional identities within discursive weather systems
- Authors: Krusche, Julianne
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: This thesis examines the professional identities of teachers in light of Australian vocational educational policy reform since the late 1980s. Although the reform has been national, this study is located within Victoria. It is specifically interested in the professional identities of Language, Literacy and Numeracy (LLN) teachers who work within the Vocational Education and Training sector. This study brings the voices of LLN specialists to the forefront. Poststructural theory, with particular emphasis on the work of Michel Foucault and narrative inquiry, is used to make sense of these voices, collectively known as the ‘voice of practice’. This study treats professional identity as a multiple term encompassing a range of assigned roles and chosen identities; as such, it argues that professional identity should be seen as fluid and dynamic and as something that continues to evolve. The teachers involved in this study actively negotiated various discourses related to the shaping of professional identity. The effects of this were threefold: LLN teachers ascribed to certain identity positions in line with government policy and institute directives; they preserved other identities; and they forged new identities based on opportunism and a resistance to policy discourse. While there has been a decline in the Adult and Community Education voice in policy development, within practice, this study found that teachers have retained a voice through the maintenance and creation of teaching practices that sit outside policy. Further, this study found that although numerous stakeholders invest in the provision of LLN, it is learners who hold a lot of the power; indeed, the needs of learners, feedback from learners and their transformative learning experiences are the primary drivers in teacher motivation and identity. Finally, this study found a misalignment between policy discourse and the voice of practice that requires attention if LLN policies are to be successfully implemented.
- Description: Doctor of Philosophy
- Authors: Krusche, Julianne
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: This thesis examines the professional identities of teachers in light of Australian vocational educational policy reform since the late 1980s. Although the reform has been national, this study is located within Victoria. It is specifically interested in the professional identities of Language, Literacy and Numeracy (LLN) teachers who work within the Vocational Education and Training sector. This study brings the voices of LLN specialists to the forefront. Poststructural theory, with particular emphasis on the work of Michel Foucault and narrative inquiry, is used to make sense of these voices, collectively known as the ‘voice of practice’. This study treats professional identity as a multiple term encompassing a range of assigned roles and chosen identities; as such, it argues that professional identity should be seen as fluid and dynamic and as something that continues to evolve. The teachers involved in this study actively negotiated various discourses related to the shaping of professional identity. The effects of this were threefold: LLN teachers ascribed to certain identity positions in line with government policy and institute directives; they preserved other identities; and they forged new identities based on opportunism and a resistance to policy discourse. While there has been a decline in the Adult and Community Education voice in policy development, within practice, this study found that teachers have retained a voice through the maintenance and creation of teaching practices that sit outside policy. Further, this study found that although numerous stakeholders invest in the provision of LLN, it is learners who hold a lot of the power; indeed, the needs of learners, feedback from learners and their transformative learning experiences are the primary drivers in teacher motivation and identity. Finally, this study found a misalignment between policy discourse and the voice of practice that requires attention if LLN policies are to be successfully implemented.
- Description: Doctor of Philosophy
Of railways, wine and flowers : A biography of George William Knight
- Authors: Hunter, Robyn
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: This thesis examines misunderstandings surrounding class and reveals Knight to have been a member of the petty bourgeoisie - aspirational, yet understanding and empathetic towards working people. Knight was an erudite, respectable gentleman, an intellectual, a man of culture, committed to liberal values and the code of the gentleman, and a defender of the oppressed. Highlighted in this study are the high tides, low ebbs and pivotal turning points that beset Knight's life. A noted writer and raconteur, he was an influential figure in Bendigo and the colony. Covering more than ninety years, Knight's story encompasses London 1832-56, early colonial Victoria to 1923, and shines a light on one of the many oft-forgotten people who helped to build the colony.
- Description: Doctor of Philosophy
- Description: The title page photograph
- Authors: Hunter, Robyn
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: This thesis examines misunderstandings surrounding class and reveals Knight to have been a member of the petty bourgeoisie - aspirational, yet understanding and empathetic towards working people. Knight was an erudite, respectable gentleman, an intellectual, a man of culture, committed to liberal values and the code of the gentleman, and a defender of the oppressed. Highlighted in this study are the high tides, low ebbs and pivotal turning points that beset Knight's life. A noted writer and raconteur, he was an influential figure in Bendigo and the colony. Covering more than ninety years, Knight's story encompasses London 1832-56, early colonial Victoria to 1923, and shines a light on one of the many oft-forgotten people who helped to build the colony.
- Description: Doctor of Philosophy
- Description: The title page photograph
Pandemic Influenza at Oodnadatta, 1919 : Aspects of treatment and care in a multiracial community
- Authors: Bullen, Heatheranne
- Date: 2018
- Type: Text , Thesis , Masters
- Full Text:
- Description: On 24 January 1919, a thirty-two-year-old nurse from Sydney, Jean Williamson, disembarked at the railway station at Oodnadatta in the far north of South Australia to commence her new role as sister in charge of the Australian Inland Mission (AIM) hostel. On 18 April that year, Williamson greeted thirty-four-year-old minister from Melbourne, Coledge Harland, who had arrived by train to take up a three-year post as padre for the AIM’s central Australian parish. Just over a month later, an influenza pandemic that had already killed untold numbers of people worldwide reached the isolated township. Drawing on primary documents, including an extensive collection of previously unseen photographs, letter and diaries from Harland and Williamson, this thesis examines the management and care of pandemic influenza at Oodnadatta from May to late July 1919. Intercultural aspects of the management and care of European, Afghan, Chinese and Aboriginal patients are examined in the context of the health and lifestyle of local residents, nursing practices, medicines, foods, accommodation and the contribution of individuals, groups and their roles. This intimate microhistory sheds light on a relatively unknown, yet important group of people in Australia’s frontier history: the missioners and others who cared for seriously ill Aboriginal and non-Aboriginal patients at Oodnadatta, provided culturally sensitive care that afforded respect, dignity and compassion to all. At the time, the gravity of the world wide situation and the sheer need to provide care saw individual efforts go unnoticed; however, in hindsight, it is possible to see and appreciate the significance of what they achieved under the most difficult of circumstances.
- Description: Masters by Research
- Authors: Bullen, Heatheranne
- Date: 2018
- Type: Text , Thesis , Masters
- Full Text:
- Description: On 24 January 1919, a thirty-two-year-old nurse from Sydney, Jean Williamson, disembarked at the railway station at Oodnadatta in the far north of South Australia to commence her new role as sister in charge of the Australian Inland Mission (AIM) hostel. On 18 April that year, Williamson greeted thirty-four-year-old minister from Melbourne, Coledge Harland, who had arrived by train to take up a three-year post as padre for the AIM’s central Australian parish. Just over a month later, an influenza pandemic that had already killed untold numbers of people worldwide reached the isolated township. Drawing on primary documents, including an extensive collection of previously unseen photographs, letter and diaries from Harland and Williamson, this thesis examines the management and care of pandemic influenza at Oodnadatta from May to late July 1919. Intercultural aspects of the management and care of European, Afghan, Chinese and Aboriginal patients are examined in the context of the health and lifestyle of local residents, nursing practices, medicines, foods, accommodation and the contribution of individuals, groups and their roles. This intimate microhistory sheds light on a relatively unknown, yet important group of people in Australia’s frontier history: the missioners and others who cared for seriously ill Aboriginal and non-Aboriginal patients at Oodnadatta, provided culturally sensitive care that afforded respect, dignity and compassion to all. At the time, the gravity of the world wide situation and the sheer need to provide care saw individual efforts go unnoticed; however, in hindsight, it is possible to see and appreciate the significance of what they achieved under the most difficult of circumstances.
- Description: Masters by Research
Physical activity promotion in physiotherapy practice
- Authors: Kunstler, Breanne
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Australian physiotherapists promote non-treatment physical activity, which is physical activity used to improve or maintain general health, to patients with musculoskeletal conditions. However, it is unclear how physiotherapists promote non-treatment physical activity and the behaviour change techniques they use to do this. This thesis used four studies to: (i) review the efficacy of physiotherapist-led physical activity interventions; (ii) investigate the factors that influence physiotherapists’ choice to promote non-treatment physical activity; and (iii) identify the behaviour change techniques that private practice and outpatient physiotherapists use to promote non-treatment physical activity. Two systematic reviews identified that physiotherapist-led physical activity interventions are efficacious. However, effects were small and not maintained. Additionally, physiotherapists only used a small number of behaviour change techniques when promoting physical activity. National survey and interview studies were used to identify the factors that influence physiotherapists’ choice to promote non-treatment physical activity. The survey found that having poor knowledge of how to promote non-treatment physical activity, prioritising other patient problems before non-treatment physical activity and using promotion methods that were not compatible with daily practice significantly and independently reduced the odds of physiotherapists promoting non-treatment physical activity. Interpretative phenomenological analysis was used to design interviews that showed that having a perceived inability to motivate an unmotivated patient and believing that patients expect hands-on therapy instead of non-treatment physical activity promotion complicated treatment choices. The behaviour change techniques Australian physiotherapists used to promote non-treatment physical activity were compared to those used to encourage adherence to rehabilitation exercises in the survey too. The survey found that physiotherapists used similar behaviour change techniques to promote non-treatment physical activity and encourage adherence to rehabilitation exercises. This thesis provides clinicians and researchers with an understanding of the factors that influence Australian physiotherapists’ decision to promote non-treatment physical activity and the behaviour change techniques they use.
- Description: Doctor of Philosophy
- Authors: Kunstler, Breanne
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Australian physiotherapists promote non-treatment physical activity, which is physical activity used to improve or maintain general health, to patients with musculoskeletal conditions. However, it is unclear how physiotherapists promote non-treatment physical activity and the behaviour change techniques they use to do this. This thesis used four studies to: (i) review the efficacy of physiotherapist-led physical activity interventions; (ii) investigate the factors that influence physiotherapists’ choice to promote non-treatment physical activity; and (iii) identify the behaviour change techniques that private practice and outpatient physiotherapists use to promote non-treatment physical activity. Two systematic reviews identified that physiotherapist-led physical activity interventions are efficacious. However, effects were small and not maintained. Additionally, physiotherapists only used a small number of behaviour change techniques when promoting physical activity. National survey and interview studies were used to identify the factors that influence physiotherapists’ choice to promote non-treatment physical activity. The survey found that having poor knowledge of how to promote non-treatment physical activity, prioritising other patient problems before non-treatment physical activity and using promotion methods that were not compatible with daily practice significantly and independently reduced the odds of physiotherapists promoting non-treatment physical activity. Interpretative phenomenological analysis was used to design interviews that showed that having a perceived inability to motivate an unmotivated patient and believing that patients expect hands-on therapy instead of non-treatment physical activity promotion complicated treatment choices. The behaviour change techniques Australian physiotherapists used to promote non-treatment physical activity were compared to those used to encourage adherence to rehabilitation exercises in the survey too. The survey found that physiotherapists used similar behaviour change techniques to promote non-treatment physical activity and encourage adherence to rehabilitation exercises. This thesis provides clinicians and researchers with an understanding of the factors that influence Australian physiotherapists’ decision to promote non-treatment physical activity and the behaviour change techniques they use.
- Description: Doctor of Philosophy
Semantic manipulation and business context in big data analytics
- Authors: Dinh, Loan
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Business organisations receive a huge amount of data from many sources every day. These data are known as big data. Since they are mostly unstructured, big data creates a complex problem of how to capture, manage, analyse and then derive meaningful information from them. To deal with the challenges that big data has brought, this research proposes a new technique in big data analytics in the business area to integrate semantically meaningful information relevant to textual queries and business context. To achieve this aim, this study makes three major related contributions. Firstly, the relationship between business processes and strategies is established using the concept of a rule-based inference model via facts and annotations. This relationship is required to determine the importance of a big data query for a business organisation. Secondly, we introduce approaches to determine the significance level of a query, by incorporating the processstrategy relationship, process contributions and priority of business strategies. Thirdly, the proposed data analytic technique embeds business context into the bedrock of data collection and analysis process. The first two contributions were implemented using Python programming language including the Pyke package (Pyke is built in the Python environment and has an artificial intelligence tool for the development of expert systems) and their performances were analysed based on a business use case. The last contribution was implemented mainly in the Hadoop and Java programs. Results show that the first contribution successfully establishes the processstrategy relationship, the second calculates the significance level of a query in relation to a business organisation, while the third reveals the huge impact of query significance level and business context on big data collection and captures deep business insights.
- Description: Doctor of Philosophy
- Authors: Dinh, Loan
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Business organisations receive a huge amount of data from many sources every day. These data are known as big data. Since they are mostly unstructured, big data creates a complex problem of how to capture, manage, analyse and then derive meaningful information from them. To deal with the challenges that big data has brought, this research proposes a new technique in big data analytics in the business area to integrate semantically meaningful information relevant to textual queries and business context. To achieve this aim, this study makes three major related contributions. Firstly, the relationship between business processes and strategies is established using the concept of a rule-based inference model via facts and annotations. This relationship is required to determine the importance of a big data query for a business organisation. Secondly, we introduce approaches to determine the significance level of a query, by incorporating the processstrategy relationship, process contributions and priority of business strategies. Thirdly, the proposed data analytic technique embeds business context into the bedrock of data collection and analysis process. The first two contributions were implemented using Python programming language including the Pyke package (Pyke is built in the Python environment and has an artificial intelligence tool for the development of expert systems) and their performances were analysed based on a business use case. The last contribution was implemented mainly in the Hadoop and Java programs. Results show that the first contribution successfully establishes the processstrategy relationship, the second calculates the significance level of a query in relation to a business organisation, while the third reveals the huge impact of query significance level and business context on big data collection and captures deep business insights.
- Description: Doctor of Philosophy
Spatial epidemiological investigation of sport and leisure injuries in Victoria, Australia
- Authors: Singh, Himalaya
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Sport and leisure injuries are recognised as a public health issue in Australia. Despite the many health benefits associated with sport and leisure participation, there is a risk of sustaining injury during participation. To keep Australia active, there is a critical need to prevent injury occurrence. Epidemiological investigations in sport and leisure injuries have been largely examined by grouping of sports, age groups, sex and level of play. In addition, intrinsic (person-level) factors have been considered, such as strength, flexibility or previous injury history. These factors may not be sufficient to identify injury burden or prevent an increase in injury incidences. In the broader injury literature (e.g., road traffic crashes or drowning), it is known that injuries often cluster within specific places (i.e., road intersections or bodies of water). These specific geographic locations may also relate to sport and leisure injuries (e.g., sports grounds or facilities). Similarly, population-level factors such as socio-economic status or cultural groups within an area could influence the types of sports and leisure activities people participate in and consequently, the injuries that occur. A review presented in this PhD thesis revealed that there is very limited sport and leisure injury epidemiological information from a geographical perspective. To address this gap, and determine whether there is a spatial pattern in sport/leisure injuries, the aim of this PhD was to examine the geospatial distribution of sport/leisure injury hospitalisations and their association with a broad range of social and economic characteristics. This thesis uses spatial epidemiological methods to answer questions such as ‘Where do sports and leisure injuries occur?’ and ‘In whom do sports/leisure injuries occur?’ The main chapters present the results of the application of spatial epidemiological methods to describe the problem, to test hypotheses and to explore associations with possible explanatory variables. The findings showed a significant variation across metropolitan, regional and rural areas in the pattern and clustering of injuries when examining different sports, age groups and other variables such as education level. A secondary aim of this thesis was to consider the dissemination of sport and injury epidemiological data. As emphasised in the literature, there is limited spatial epidemiological information available to decision-makers and key stakeholders. At best, descriptive maps might be included in a report or research paper. However, these are static and limited to the results that the author chooses to present. Therefore, an important output from this PhD is a web-GIS application that has been specifically built to enable the exploratory analysis of sport/leisure injuries in Victoria. Sport and leisure injury prevention strategies and policy development relies on information about where, when, to whom and how sport/leisure injuries occur. This thesis demonstrates that a spatial epidemiological approach is an important and novel way to address epidemiological questions from a geographical perspective.
- Description: Doctor of Philosophy
- Authors: Singh, Himalaya
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Sport and leisure injuries are recognised as a public health issue in Australia. Despite the many health benefits associated with sport and leisure participation, there is a risk of sustaining injury during participation. To keep Australia active, there is a critical need to prevent injury occurrence. Epidemiological investigations in sport and leisure injuries have been largely examined by grouping of sports, age groups, sex and level of play. In addition, intrinsic (person-level) factors have been considered, such as strength, flexibility or previous injury history. These factors may not be sufficient to identify injury burden or prevent an increase in injury incidences. In the broader injury literature (e.g., road traffic crashes or drowning), it is known that injuries often cluster within specific places (i.e., road intersections or bodies of water). These specific geographic locations may also relate to sport and leisure injuries (e.g., sports grounds or facilities). Similarly, population-level factors such as socio-economic status or cultural groups within an area could influence the types of sports and leisure activities people participate in and consequently, the injuries that occur. A review presented in this PhD thesis revealed that there is very limited sport and leisure injury epidemiological information from a geographical perspective. To address this gap, and determine whether there is a spatial pattern in sport/leisure injuries, the aim of this PhD was to examine the geospatial distribution of sport/leisure injury hospitalisations and their association with a broad range of social and economic characteristics. This thesis uses spatial epidemiological methods to answer questions such as ‘Where do sports and leisure injuries occur?’ and ‘In whom do sports/leisure injuries occur?’ The main chapters present the results of the application of spatial epidemiological methods to describe the problem, to test hypotheses and to explore associations with possible explanatory variables. The findings showed a significant variation across metropolitan, regional and rural areas in the pattern and clustering of injuries when examining different sports, age groups and other variables such as education level. A secondary aim of this thesis was to consider the dissemination of sport and injury epidemiological data. As emphasised in the literature, there is limited spatial epidemiological information available to decision-makers and key stakeholders. At best, descriptive maps might be included in a report or research paper. However, these are static and limited to the results that the author chooses to present. Therefore, an important output from this PhD is a web-GIS application that has been specifically built to enable the exploratory analysis of sport/leisure injuries in Victoria. Sport and leisure injury prevention strategies and policy development relies on information about where, when, to whom and how sport/leisure injuries occur. This thesis demonstrates that a spatial epidemiological approach is an important and novel way to address epidemiological questions from a geographical perspective.
- Description: Doctor of Philosophy
Stay or go? Young people’s agency and mobility in and out of small towns
- Authors: Parkin, Ember
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: This doctoral thesis examines young people’s place attachments in two small Victorian towns. This qualitative ethnographic study uses auto-driven photo-elicitation to understand young people’s sense of place and futures in their home towns of Castlemaine and Maryborough. These case study towns are of a similar size, geography and heritage fabric. However, they are home to starkly different social indicators and economic policy contexts. The study seeks to understand how the cultural features of small towns affect young people’s place attachment and also how place relationships might subsequently affect young people’s sense of futures through their desired and intended locations and aspirations. To achieve this, the thesis explores young people’s social constructions of place. The photoelicitation method enables close attention to be paid to young people’s engagement with their home towns. This thesis argues that agency or lack of agency is a significant factor in strengthening or diminishing young people’s place attachments. Previous research suggests that one result of place attachment is that people will seek to remain being in a place. For young people in this study there appears to be an inverse relationship. Young people who had a broad and holistic sense of place engagement and attachment also had a broad sense of future possibilities and thus, intended to leave their home towns in pursuit of personal growth and education. Whereas young people who had a more limited sense of attachment or engagement had a narrower sense of future possibilities and were less likely to desire to leave their home town. The study contributes to knowledge about the ways in which place engagement can affect young people’s social and physical mobility.
- Description: Doctor of Philosophy
- Authors: Parkin, Ember
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: This doctoral thesis examines young people’s place attachments in two small Victorian towns. This qualitative ethnographic study uses auto-driven photo-elicitation to understand young people’s sense of place and futures in their home towns of Castlemaine and Maryborough. These case study towns are of a similar size, geography and heritage fabric. However, they are home to starkly different social indicators and economic policy contexts. The study seeks to understand how the cultural features of small towns affect young people’s place attachment and also how place relationships might subsequently affect young people’s sense of futures through their desired and intended locations and aspirations. To achieve this, the thesis explores young people’s social constructions of place. The photoelicitation method enables close attention to be paid to young people’s engagement with their home towns. This thesis argues that agency or lack of agency is a significant factor in strengthening or diminishing young people’s place attachments. Previous research suggests that one result of place attachment is that people will seek to remain being in a place. For young people in this study there appears to be an inverse relationship. Young people who had a broad and holistic sense of place engagement and attachment also had a broad sense of future possibilities and thus, intended to leave their home towns in pursuit of personal growth and education. Whereas young people who had a more limited sense of attachment or engagement had a narrower sense of future possibilities and were less likely to desire to leave their home town. The study contributes to knowledge about the ways in which place engagement can affect young people’s social and physical mobility.
- Description: Doctor of Philosophy
Susceptibility of rehabilitated mine batter surface to mass movement
- Authors: Allen, Tristan
- Date: 2018
- Type: Text , Thesis , Masters
- Full Text:
- Description: The goal of the research is to quantify coal properties that may affect the processes and controls governing rehabilitated brown coal mine surface mass movements. The research investigates weathering of coal and assesses the difference in strength characteristics between fresh and weathered coal. In addition to quantifying the mechanical properties of coal surfaces in a rehabilitated slope, permeability changes due to weathering of coal are also investigated. Changes in coal strength influence sliding resistance. Changes in coal permeability impact pore pressures above the coal surface, which may also affect sliding resistance on the coal – cover interface. To assess these issues, direct and residual shear tests were used to investigate the changes in shear strength due to weathering at low normal stresses applicable to shallow cover materials. Testing was undertaken with abrasive surfaces to simulate sliding on the contact coal surface beneath cover materials assuming that the cover material is stronger than the coal. The roughness of the abrasive surface proved to be unimportant for large strain shear strength. The shear strength for coal with different weathering and normal effective stresses was examined. Coal cohesion was found to be low, but some rebinding of coal would occur with time. A coal residual friction angle of 39.1 and 37.0 degrees was found for the unsaturated and saturated tested coal respectively. Permeability tests using oxygenated water were undertaken to investigate changes to brown coal permeability as a result of weathering. Even with low levels of oxidation achievable with the permeability test apparatus, coal permeability dropped over time. While the magnitude of the reduction was not large for low oxidation magnitudes, the impact on permeability was demonstrated. A weathering index was developed as part of the study to provide a quantitative basis for assessing the weathered state of coal samples. The index employed changes to Fourier Transform Infrared Spectroscopy (FTIR) spectra to define the state of weathering. To assess the rate and magnitude of weathering of coal through oxidation an autoclave was used to artificially weather brown coal. Fourier Transform Infrared Spectroscopy and Gas Chromatography used to analyse the results. As for the permeability testing the autoclave experiments could not be run for sufficient time to progress to complete weathering by oxidation. Nevertheless the principles of the test and the equipment specifications were developed so that they could be used in future to complete the determination of weathering rates. The research has demonstrated the importance of understanding coal weathering at the upper boundary of a rehabilitated coal surface to the potential for cover mass movements due to sliding at the coal cover interface.
- Description: Masters by Research
- Authors: Allen, Tristan
- Date: 2018
- Type: Text , Thesis , Masters
- Full Text:
- Description: The goal of the research is to quantify coal properties that may affect the processes and controls governing rehabilitated brown coal mine surface mass movements. The research investigates weathering of coal and assesses the difference in strength characteristics between fresh and weathered coal. In addition to quantifying the mechanical properties of coal surfaces in a rehabilitated slope, permeability changes due to weathering of coal are also investigated. Changes in coal strength influence sliding resistance. Changes in coal permeability impact pore pressures above the coal surface, which may also affect sliding resistance on the coal – cover interface. To assess these issues, direct and residual shear tests were used to investigate the changes in shear strength due to weathering at low normal stresses applicable to shallow cover materials. Testing was undertaken with abrasive surfaces to simulate sliding on the contact coal surface beneath cover materials assuming that the cover material is stronger than the coal. The roughness of the abrasive surface proved to be unimportant for large strain shear strength. The shear strength for coal with different weathering and normal effective stresses was examined. Coal cohesion was found to be low, but some rebinding of coal would occur with time. A coal residual friction angle of 39.1 and 37.0 degrees was found for the unsaturated and saturated tested coal respectively. Permeability tests using oxygenated water were undertaken to investigate changes to brown coal permeability as a result of weathering. Even with low levels of oxidation achievable with the permeability test apparatus, coal permeability dropped over time. While the magnitude of the reduction was not large for low oxidation magnitudes, the impact on permeability was demonstrated. A weathering index was developed as part of the study to provide a quantitative basis for assessing the weathered state of coal samples. The index employed changes to Fourier Transform Infrared Spectroscopy (FTIR) spectra to define the state of weathering. To assess the rate and magnitude of weathering of coal through oxidation an autoclave was used to artificially weather brown coal. Fourier Transform Infrared Spectroscopy and Gas Chromatography used to analyse the results. As for the permeability testing the autoclave experiments could not be run for sufficient time to progress to complete weathering by oxidation. Nevertheless the principles of the test and the equipment specifications were developed so that they could be used in future to complete the determination of weathering rates. The research has demonstrated the importance of understanding coal weathering at the upper boundary of a rehabilitated coal surface to the potential for cover mass movements due to sliding at the coal cover interface.
- Description: Masters by Research
Teachers' emotional intelligence as a predisposition for discrimiation against students with severe emotional and behavioural disorders
- Authors: Metaxas, Melinda
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Students with Emotional Behavioural Disorders (EBD) are among the most challenging students to teach. Personality Trait Theory predicts teachers’ level of Emotional Intelligence (EI) would affect their cognitive-affective-behavioural reactions towards students with EBDs, and influence level of academic achievement and difficult behaviour of these students. This research explores which teachers are more predisposed to discriminate against EBD students, and identifies the most ‘effective’, supportive EI teacher traits. Underlying psychological processes, such as genetic EI make-up of teachers, may prove to be most valuable in determining whether more practical strategies for dealing with students’ behaviour/emotions are effectively applied and successful. An Attribution Model framework helped assess teacher reactions towards students. Two hundred and sixty one teachers from 51 Victorian schools participated in the study by completing self-report questionnaires, including the Trait Emotional Intelligence Questionnaire. A quantitative survey methodology used vignettes, with each school contact person randomly giving 50/50 surveys to their teachers (depicting a student with either mild or severe EBD symptoms). Pathway analysis revealed that teachers with higher EI reported less stigmatising and punitive intentions and likely greater helping behaviours. A new EI Process Model of Stigmatisation was proposed to measure teacher reactions through an affective-cognitive-behavioural sequence, rather than a cognitive-affective-behavioural sequence. Teachers’ EI levels also related to their own levels of psychological distress and/or compassion stress, which influenced likely helping or punitive outcomes. Despite behavioural severity of EBD students, teachers higher in EI still indicate more supportive helping behaviours. Specifically-identified ‘ideal’ teacher EI traits should lead to greater helping and be psychologically beneficial to both students and teachers. These results assisted development of an assessment tool (ASET – Assessment Screen for Emotionally Intelligent Teachers), which lays a sound foundation for schools and others to profile or recruit teachers with best ‘qualities’ to effectively teach students EBD students.
- Description: Doctor of Philosophy
- Authors: Metaxas, Melinda
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Students with Emotional Behavioural Disorders (EBD) are among the most challenging students to teach. Personality Trait Theory predicts teachers’ level of Emotional Intelligence (EI) would affect their cognitive-affective-behavioural reactions towards students with EBDs, and influence level of academic achievement and difficult behaviour of these students. This research explores which teachers are more predisposed to discriminate against EBD students, and identifies the most ‘effective’, supportive EI teacher traits. Underlying psychological processes, such as genetic EI make-up of teachers, may prove to be most valuable in determining whether more practical strategies for dealing with students’ behaviour/emotions are effectively applied and successful. An Attribution Model framework helped assess teacher reactions towards students. Two hundred and sixty one teachers from 51 Victorian schools participated in the study by completing self-report questionnaires, including the Trait Emotional Intelligence Questionnaire. A quantitative survey methodology used vignettes, with each school contact person randomly giving 50/50 surveys to their teachers (depicting a student with either mild or severe EBD symptoms). Pathway analysis revealed that teachers with higher EI reported less stigmatising and punitive intentions and likely greater helping behaviours. A new EI Process Model of Stigmatisation was proposed to measure teacher reactions through an affective-cognitive-behavioural sequence, rather than a cognitive-affective-behavioural sequence. Teachers’ EI levels also related to their own levels of psychological distress and/or compassion stress, which influenced likely helping or punitive outcomes. Despite behavioural severity of EBD students, teachers higher in EI still indicate more supportive helping behaviours. Specifically-identified ‘ideal’ teacher EI traits should lead to greater helping and be psychologically beneficial to both students and teachers. These results assisted development of an assessment tool (ASET – Assessment Screen for Emotionally Intelligent Teachers), which lays a sound foundation for schools and others to profile or recruit teachers with best ‘qualities’ to effectively teach students EBD students.
- Description: Doctor of Philosophy
Tertiary student connectedness : Intervention influence on student connectedness as measured in health and academic behaviours of regional tertiary students
- Authors: Young, Patricia
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: At a time when university student diversity is heightened and when national government regulations have shifted their quality focus from support improvements to student achievement, little is known about the mechanism of the latter. University efforts to support quality, caring interactions between students and staff, known as connectedness, are currently favoured, and this thesis examines connectedness, mood, emotional well-being and academic behaviours of commencing students at a regional Australian university. This is done through the lens of a first semester intervention, known as Tertiary Learning Communities (TLC). Drawing on existing connectedness research, across both school and university settings and guided by a whole-school framework, details of the establishment of a suitable working party, the development of survey, pilot and intervention activities, and monitoring the ability of a cross-campus intervention to influence student connectedness and behaviour markers, are highlighted to advance further understanding of the mechanics of connectedness in a university setting. The developed survey, which collected data from undergraduate students early in their first semester, effectively captured perceptions of connectedness across a broad range of sources in addition to their mood, emotional well-being and academic student behaviours. Paired sample-tests assessed connectedness changes, and chi square analysis assessed behaviour changes when comparing the experimental and control groups on two occasions. A single intervention aligned to a first year core unit to support academic and social interactions, was shown to be ineffective in enhancing student connectedness during the semester of the intervention or the semester following the intervention. However, the level of connectedness decreases measured across ‘personal’, ‘other students’ and ‘lecturers’ were indeed significant, as was the finding that connectedness decreases for intervention participants exceeded the decreases of the control group. Furthermore, mood and emotional well-being challenges and the slow emergence of academic behaviours were also revealed. These findings provided support for future inclusive student support initiatives, maintained the involvement of working party members and extended support beyond the first semester to across first year.
- Description: Doctor of Philosophy
- Authors: Young, Patricia
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: At a time when university student diversity is heightened and when national government regulations have shifted their quality focus from support improvements to student achievement, little is known about the mechanism of the latter. University efforts to support quality, caring interactions between students and staff, known as connectedness, are currently favoured, and this thesis examines connectedness, mood, emotional well-being and academic behaviours of commencing students at a regional Australian university. This is done through the lens of a first semester intervention, known as Tertiary Learning Communities (TLC). Drawing on existing connectedness research, across both school and university settings and guided by a whole-school framework, details of the establishment of a suitable working party, the development of survey, pilot and intervention activities, and monitoring the ability of a cross-campus intervention to influence student connectedness and behaviour markers, are highlighted to advance further understanding of the mechanics of connectedness in a university setting. The developed survey, which collected data from undergraduate students early in their first semester, effectively captured perceptions of connectedness across a broad range of sources in addition to their mood, emotional well-being and academic student behaviours. Paired sample-tests assessed connectedness changes, and chi square analysis assessed behaviour changes when comparing the experimental and control groups on two occasions. A single intervention aligned to a first year core unit to support academic and social interactions, was shown to be ineffective in enhancing student connectedness during the semester of the intervention or the semester following the intervention. However, the level of connectedness decreases measured across ‘personal’, ‘other students’ and ‘lecturers’ were indeed significant, as was the finding that connectedness decreases for intervention participants exceeded the decreases of the control group. Furthermore, mood and emotional well-being challenges and the slow emergence of academic behaviours were also revealed. These findings provided support for future inclusive student support initiatives, maintained the involvement of working party members and extended support beyond the first semester to across first year.
- Description: Doctor of Philosophy
The acute effects of aerobic exercise on Leukocyte Telomere biology
- Authors: Chilton, Warrick
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Doctor of Philosophy
- Description: Habitual exercise is unequivocally associated with decreased all-cause mortality and morbidity. Despite the strength of the association, a large part of the decreased risk is physiologically unaccounted for. Accumulating evidence indicates that leukocyte telomere length (LTL) may be one such explanatory mechanism. Telomeres are specialized deoxyribonucleic acid (DNA) sequences located at chromosomal ends where they protect the genomic DNA from enzymatic degradation. Excessive and/or premature telomere shortening in leukocytes is associated with a host of chronic diseases and impaired immune function. Observational associations exist between LTL and habitual physical activity/exercise in multiple cohorts. However, correlation does not imply causal story and the underpinning mechanisms behind the association are unclear. The current consensus is that long-term exercise-induced reductions in oxidative stress and inflammation mediate the association. The acute dynamics of telomere biology are poorly understood; however, a growing body of evidence suggests that telomeres may be amenable to acute modulation via expression of telomereassociated genes and microRNAs. Accordingly, the overarching aim of this thesis was to characterize the acute effects of aerobic exercise on leukocyte telomere biology.
- Authors: Chilton, Warrick
- Date: 2018
- Type: Text , Thesis , PhD
- Full Text:
- Description: Doctor of Philosophy
- Description: Habitual exercise is unequivocally associated with decreased all-cause mortality and morbidity. Despite the strength of the association, a large part of the decreased risk is physiologically unaccounted for. Accumulating evidence indicates that leukocyte telomere length (LTL) may be one such explanatory mechanism. Telomeres are specialized deoxyribonucleic acid (DNA) sequences located at chromosomal ends where they protect the genomic DNA from enzymatic degradation. Excessive and/or premature telomere shortening in leukocytes is associated with a host of chronic diseases and impaired immune function. Observational associations exist between LTL and habitual physical activity/exercise in multiple cohorts. However, correlation does not imply causal story and the underpinning mechanisms behind the association are unclear. The current consensus is that long-term exercise-induced reductions in oxidative stress and inflammation mediate the association. The acute dynamics of telomere biology are poorly understood; however, a growing body of evidence suggests that telomeres may be amenable to acute modulation via expression of telomereassociated genes and microRNAs. Accordingly, the overarching aim of this thesis was to characterize the acute effects of aerobic exercise on leukocyte telomere biology.