Cloning, expression, purification and crystallographic studies of galectin-11 from domestic sheep (Ovis aries)
- Sakthivel, Dhanasekaran, Littler, Dene, Shahine, Adam, Troy, Sally, Johnson, Matthew, Rossjohn, Jamie, Piedrafita, David, Beddoe, Travis
- Authors: Sakthivel, Dhanasekaran , Littler, Dene , Shahine, Adam , Troy, Sally , Johnson, Matthew , Rossjohn, Jamie , Piedrafita, David , Beddoe, Travis
- Date: 2015
- Type: Text , Journal article
- Relation: Acta Crystallographica Section:F Structural Biology Communications Vol. 71, no. (2015), p. 993-997
- Full Text:
- Reviewed:
- Description: Galectins are an evolutionarily conserved family of proteins that translate glycan recognition into cellular effects. Galectin-11 is a unique member of the galectin family that is only expressed in ruminants such as sheep, goat and cattle and that plays a critical role in several important biological processes, such as reproduction and parasite-mediated innate immune responses. Currently, these two areas are of major importance for the sustainability of ruminant livestock production. Despite the emerging biological significance of galectin-11, no structural information is available. It is expected that structural studies will unravel the functional mechanisms of galectin-11 activity. Here, the expression, purification and crystallization of the ruminant-specific galectin-11 from domestic sheep and the collection of X-ray data to 2.0 Å resolution are reported. © 2015.
- Authors: Sakthivel, Dhanasekaran , Littler, Dene , Shahine, Adam , Troy, Sally , Johnson, Matthew , Rossjohn, Jamie , Piedrafita, David , Beddoe, Travis
- Date: 2015
- Type: Text , Journal article
- Relation: Acta Crystallographica Section:F Structural Biology Communications Vol. 71, no. (2015), p. 993-997
- Full Text:
- Reviewed:
- Description: Galectins are an evolutionarily conserved family of proteins that translate glycan recognition into cellular effects. Galectin-11 is a unique member of the galectin family that is only expressed in ruminants such as sheep, goat and cattle and that plays a critical role in several important biological processes, such as reproduction and parasite-mediated innate immune responses. Currently, these two areas are of major importance for the sustainability of ruminant livestock production. Despite the emerging biological significance of galectin-11, no structural information is available. It is expected that structural studies will unravel the functional mechanisms of galectin-11 activity. Here, the expression, purification and crystallization of the ruminant-specific galectin-11 from domestic sheep and the collection of X-ray data to 2.0 Å resolution are reported. © 2015.
High expression of oleoyl-ACP hydrolase underpins life-threatening respiratory viral diseases
- Jia, Xiaoxiao, Crawford, Jeremy, Gebregzabher, Deborah, Monson, Ebony, Mettelman, Robert, Wan, Yanmin, Ren, Yanqin, Chou, Janet, Novak, Tanya, McQuilten, Hayley, Clarke, Michele, Bachem, Annabell, Foo, Isabelle, Fritzlar, Svenja, Carrera Montoya, Julio, Trenerry, Alice, Nie, Shuai, Leeming, Michael, Nguyen, Thi, Kedzierski, Lukasz, Littler, Dene, Kueh, Andrew, Cardamone, Tina, Wong, Chinn, Hensen, Luca, Cabug, Aira, Laguna, Jaime, Agrawal, Mona, Flerlage, Tim, Berzins, Stuart
- Authors: Jia, Xiaoxiao , Crawford, Jeremy , Gebregzabher, Deborah , Monson, Ebony , Mettelman, Robert , Wan, Yanmin , Ren, Yanqin , Chou, Janet , Novak, Tanya , McQuilten, Hayley , Clarke, Michele , Bachem, Annabell , Foo, Isabelle , Fritzlar, Svenja , Carrera Montoya, Julio , Trenerry, Alice , Nie, Shuai , Leeming, Michael , Nguyen, Thi , Kedzierski, Lukasz , Littler, Dene , Kueh, Andrew , Cardamone, Tina , Wong, Chinn , Hensen, Luca , Cabug, Aira , Laguna, Jaime , Agrawal, Mona , Flerlage, Tim , Berzins, Stuart
- Date: 2024
- Type: Text , Journal article
- Relation: Cell Vol. 187, no. 17 (2024), p. 4586-4604.e20
- Full Text:
- Reviewed:
- Description: Respiratory infections cause significant morbidity and mortality, yet it is unclear why some individuals succumb to severe disease. In patients hospitalized with avian A(H7N9) influenza, we investigated early drivers underpinning fatal disease. Transcriptomics strongly linked oleoyl-acyl-carrier-protein (ACP) hydrolase (OLAH), an enzyme mediating fatty acid production, with fatal A(H7N9) early after hospital admission, persisting until death. Recovered patients had low OLAH expression throughout hospitalization. High OLAH levels were also detected in patients hospitalized with life-threatening seasonal influenza, COVID-19, respiratory syncytial virus (RSV), and multisystem inflammatory syndrome in children (MIS-C) but not during mild disease. In olah
- Authors: Jia, Xiaoxiao , Crawford, Jeremy , Gebregzabher, Deborah , Monson, Ebony , Mettelman, Robert , Wan, Yanmin , Ren, Yanqin , Chou, Janet , Novak, Tanya , McQuilten, Hayley , Clarke, Michele , Bachem, Annabell , Foo, Isabelle , Fritzlar, Svenja , Carrera Montoya, Julio , Trenerry, Alice , Nie, Shuai , Leeming, Michael , Nguyen, Thi , Kedzierski, Lukasz , Littler, Dene , Kueh, Andrew , Cardamone, Tina , Wong, Chinn , Hensen, Luca , Cabug, Aira , Laguna, Jaime , Agrawal, Mona , Flerlage, Tim , Berzins, Stuart
- Date: 2024
- Type: Text , Journal article
- Relation: Cell Vol. 187, no. 17 (2024), p. 4586-4604.e20
- Full Text:
- Reviewed:
- Description: Respiratory infections cause significant morbidity and mortality, yet it is unclear why some individuals succumb to severe disease. In patients hospitalized with avian A(H7N9) influenza, we investigated early drivers underpinning fatal disease. Transcriptomics strongly linked oleoyl-acyl-carrier-protein (ACP) hydrolase (OLAH), an enzyme mediating fatty acid production, with fatal A(H7N9) early after hospital admission, persisting until death. Recovered patients had low OLAH expression throughout hospitalization. High OLAH levels were also detected in patients hospitalized with life-threatening seasonal influenza, COVID-19, respiratory syncytial virus (RSV), and multisystem inflammatory syndrome in children (MIS-C) but not during mild disease. In olah
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