- Title
- Distinct Streptococcus pneumoniae cause invasive disease in Papua New Guinea
- Creator
- Mellor, Kate; Lo, Stephanie; Yoannes, Mition; Michael, Audrey; Orami, Tilda; Greenhill, Andrew; Breiman, Robert; Hawkins, Paulina; McGee, Lesley; Bentley, Stephen; Ford, Rebecca; Lehmann, Deborah
- Date
- 2022
- Type
- Text; Journal article
- Identifier
- http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/189483
- Identifier
- vital:17456
- Identifier
-
https://doi.org/10.1099/mgen.0.000835
- Identifier
- ISSN:2057-5858 (ISSN)
- Abstract
- Streptococcus pneumoniae is a key contributor to childhood morbidity and mortality in Papua New Guinea (PNG). For the first time, whole genome sequencing of 174 isolates has enabled detailed characterisation of diverse S. pneumoniae causing invasive disease in young children in PNG, 1989-2014. This study captures the baseline S. pneumoniae population prior to the introduction of 13-valent pneumococcal conjugate vaccine (PCV13) into the national childhood immunisation programme in 2014. Relationships amongst lineages, serotypes and antimicrobial resistance traits were characterised, and the population was viewed in the context of a global collection of isolates. The analyses highlighted adiverse S. pneumoniae population associated with invasive disease in PNG, with 45 unique Global Pneumococcal Sequence Clusters (GPSCs) observed amongst the 174 isolates reflecting multiple lineages observed in PNG that have not been identified in other geographic locations. The majority of isolates were from children with meningitis, of which 52% (n=72) expressed non-PCV13 serotypes. Over a third of isolates were predicted to be resistant to at least one antimicrobial. PCV13 serotype isolates had 10.1 times the odds of being multidrug-resistant (MDR) compared to non-vaccine serotype isolates, and no isolates with GPSCs unique to PNG were MDR. Serotype 2 was the most commonly identified serotype; we identified a highly clonal cluster of serotype 2 isolates unique to PNG, and a distinct second cluster indicative of long-distance transmission. Ongoing surveillance, including whole-genome sequencing, is needed to ascertain the impact of the national PCV13 programme upon the S. pneumoniae population, including serotype replacement and antimicrobial resistance traits. © 2022 The Authors.
- Publisher
- Microbiology Society
- Relation
- Microbial Genomics Vol. 8, no. 7 (2022), p.
- Rights
- All metadata describing materials held in, or linked to, the repository is freely available under a CC0 licence
- Rights
- https://creativecommons.org/licenses/by/4.0/
- Rights
- Copyright © 2022 The Authors
- Rights
- Open Access
- Subject
- 3105 Genetics; 3107 Microbiology; Invasive disease; Papua New Guinea; Streptococcus pneumoniae
- Full Text
- Reviewed
- Funder
- This study was co-funded by the Bill and Melinda Gates Foundation (grant code OPP1034556), the Wellcome Sanger Institute (core Wellcome grants 098051 and 206194), and the USA Centres for Disease Control and Prevention. The funding sources had no role in isolate selection, analysis, or data interpretation.
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