- Title
- Metabolic profiling and in vitro assessment of anthelmintic fractions of Picria fel-terrae Lour
- Creator
- Kumarasingha, Rasika; Karpe, Avinash; Preston, Sarah; Yeo, Tiong-Chia; Lim, Diana; Tu, Chu-Lee; Luu, Jennii; Simpson, Kaylene; Shaw, Jillian; Gasser, Robin; Beale, David; Morrison, Paul; Palombo, Enzo; Boag, Peter
- Date
- 2016
- Type
- Text; Journal article
- Identifier
- http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/167827
- Identifier
- vital:13736
- Identifier
-
https://doi.org/10.1016/j.ijpddr.2016.08.002
- Identifier
- ISBN:2211-3207
- Abstract
- Anthelmintic resistance is widespread in gastrointestinal nematode populations, such that there is a consistent need to search for new anthelmintics. However, the cost of screening for new compounds is high and has a very low success rate. Using the knowledge of traditional healers from Borneo Rainforests (Sarawak, Malaysia), we have previously shown that some traditional medicinal plants are a rich source of potential new anthelmintic drug candidates. In this study, Picria fel-terrae Lour. plant extract, which has previously shown promising anthelmintic activities, was fractionated via the use of a solid phase extraction cartridge and each isolated fraction was then tested on free-living nematode Caenorhabditis elegans and the parasitic nematode Haemonchus contortus. We found that a single fraction was enriched for nematocidal activity, killing ≥90% of C. elegans adults and inhibiting the motility of exsheathed L3 of H. contortus, while having minimal cytotoxic activity in mammalian cell culture. Metabolic profiling and chemometric analysis of the effective fraction indicated medium chained fatty acids and phenolic acids were highly represented. Image 1 •Chemical fractionation of Picria fel-terrae Lour. plant extract.•Anthelmintic activity against Caenorhabditis elegans and Haemonchus contortus.•Metabolic profiling and chemometric analysis of active fraction.•Active fraction has minimal mammalian cytotoxicity.
- Relation
- International Journal for Parasitology: Drugs and Drug Resistance Vol. 6, no. 3 (2016), p. 171-178
- Rights
- http://creativecommons.org/licenses/by-nc-nd/4
- Rights
- Crown Copyright ©2016 Published by Elsevier Ltd on behalf of Australian Society for Parasitology. This is an open access article under the CC BY-NC-ND license
- Rights
- Open Access
- Rights
- This metadata is freely available under a CCO license
- Subject
- Picria Fel-Terrae Lour.; Caenorhabditis Elegans; Haemonchus Contortus; Metabolomics; Traditional Medicines; 0605 Microbiology; 0608 Zoology; 0707 Veterinary Sciences
- Full Text
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