- Title
- Dexamethasone leads to Zn2+ accumulation and increased unbound Zn2+ in C2C12 muscle and 3T3-L1 adipose cells
- Creator
- Maier, Michelle; Nankervis, Scott; Wallace, Morgan; Develyn, Tamekha; Myers, Mark
- Date
- 2023
- Type
- Text; Journal article
- Identifier
- http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/193676
- Identifier
- vital:18212
- Identifier
-
https://doi.org/10.1002/jcb.30376
- Identifier
- ISSN:0730-2312 (ISSN)
- Abstract
- Skeletal muscle atrophy is associated with increases in circulating glucocorticoid levels and insulin resistance. Zinc accumulates in atrophic muscle, but the relationship between atrophy, insulin resistance, and Zn2+ homeostasis remains unclear. In this study, the effect of the glucocorticoid dexamethasone (DEX) on insulin and Zn2+ homeostasis was explored. Treatment of differentiated C2C12 skeletal myotubes and 3T3-L1 adipocytes with DEX significantly increased mRNA expression of the metal-binding proteins Mt1 and 2 and altered energy storage as shown by the increased size of lipid droplets in 3T3-L1 cells. In C2C12 cells the total cellular Zn2+ was higher after DEX treatment, and in both C2C12 and 3T3-L1 adipocytes, free unbound Zn2+ was increased. Insulin treatment led to a gradual increase in free Zn2+ in C2C12 cells, and no significant change in DEX-treated cells such that concentrations were similar 10 min after insulin treatment. These data demonstrate that DEX disturbs Zn2+ homeostasis in muscle and fat cells. Further study of the molecular pathways involved to identify novel therapeutic targets for treatment of skeletal muscle atrophy is warranted. © 2023 The Authors. Journal of Cellular Biochemistry published by Wiley Periodicals LLC.
- Publisher
- John Wiley and Sons Inc
- Relation
- Journal of Cellular Biochemistry Vol. 124, no. 3 (2023), p. 409-420
- Rights
- All metadata describing materials held in, or linked to, the repository is freely available under a CC0 licence
- Rights
- Copyright © 2023 The Authors
- Rights
- Open Access
- Rights
- https://creativecommons.org/licenses/by/4.0/
- Subject
- 3101 Biochemistry and cell biology; Dexamethasone; Glucocorticoids; Insulin; Zinc
- Full Text
- Reviewed
- Funder
- Open access publishing facilitated by Federation University Australia, as part of the Wiley - Federation University Australia agreement via the Council of Australian University Librarians.
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