- Title
- The optimization of loop-mediated isothermal amplification (LAMP) as a diagnostic tool for low-density, asymptomatic malaria infections
- Creator
- Surrao, Leanna
- Date
- 2020
- Type
- Text; Thesis; PhD
- Identifier
- http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/172068
- Identifier
- vital:14458
- Abstract
- Current diagnostic tools for malaria lack the sensitivity to identify individuals with low-density infections. Asymptomatic low-density infections are common in malaria endemic regions and these individuals provide an important reservoir of infection that enables transmission to mosquitoes. Failure to detect these individuals threatens the global health goal of malaria elimination. Loop-mediated isothermal amplification (LAMP) is a technique to amplify DNA and has the potential to diagnose these individuals. The LAMP assay was assessed in a field study in rural Vietnam. 5421 samples were collected and tested with a commercially available LAMP assay in Commune Health Care Centres in Binh Phuoc and Dak Nong Provinces. 101 positive LAMP cases (asymptomatic, smear, and RDT negative) were identified, with the proportion of positives ranging from 0.18% and 3.25% across five communes. In order for LAMP to be used as a screening tool, it must be cost effective and have a workflow suitable for minimally trained end users. To achieve this, an in-house LAMP assay was developed and compared to PCR. The assay was combined with instrument detection to simplify decision making for the end user and improve sensitivity. The in-house assay was as sensitive as the PCR assay and cost US$0.60 per reaction compared to US$3.57 for PCR and US$8.23 for the commercial LAMP. An integrated single cartridge, called T1, was assessed to further simplify this workflow of sample preparation, LAMP amplification and detection. Further development of the cartridge and the assay will be required for future deployment. The LAMP assay is suitable for detection of low density infections in asymptomatically infected individuals in field settings and has the potential for cost effective population based screening.; Doctor of Philosophy
- Publisher
- Federation University Australia
- Rights
- Copyright Leanna Surrao
- Rights
- Open Access
- Rights
- This metadata is freely available under a CCO license
- Subject
- Loop-mediated isothermal amplification (LAMP); Diagnostic; Asymptomatic; Malaria; High-sensitivity
- Full Text
- Thesis Supervisor
- Andrew Greenhill
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