Leukocyte telomere length variation due to DNA extraction method

Title: Leukocyte telomere length variation due to DNA extraction method
Creator: Denham, Joshua; Marques, Francine; Charchar, Fadi
Date: 2014
Type: Text; Journal article
Identifier: http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/167900
Identifier: vital:13727
Identifier: https://doi.org/10.1186/1756-0500-7-877
Identifier: ISBN:1756-0500
Abstract: Telomere length is indicative of biological age. Shorter telomeres have been associated with several disease and health states. There are inconsistencies throughout the literature amongst relative telomere length measured by quantitative PCR (qPCR) and different extraction methods or kits used. We quantified whole-blood leukocyte telomere length using the telomere to single copy gene (T/S) ratio by qPCR in 20 young (18-25 yrs) men after extracting DNA using three common extraction methods: Lahiri and Nurnberger (high salt) method, PureLink Genomic DNA Mini kit (Life Technologies) and QiaAmp DNA Mini kit (Qiagen). Telomere length differences of DNA extracted from the three extraction methods was assessed by one-way analysis of variance (ANOVA).
Relation: BMC research notes Vol. 7, no. (2014), p. 877
Rights: http://creativecommons.org/licenses/by/4.0
Rights: Open Access
Rights: This metadata is freely available under a CCO license
Subject: Telomere; DNA; Leukocytes; 1199 Other Medical and Health Sciences
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