- Title
- Neurotrophic biomarker change after physical activity and mindfulness interventions
- Creator
- England, Gina
- Date
- 2017
- Type
- Text; Thesis; Masters
- Identifier
- http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/166439
- Identifier
- vital:13425
- Identifier
- https://library.federation.edu.au/record=b2743566
- Abstract
- BACKGROUND AND AIM AND HYPOTHESIS: BDNF, FGF2 and NGF are neurotrophins associated with neuroplasticity, nervous system development and psychiatric disorder in the literature. BDNF in particular is suggested as a useful biomarker of mood disorder. Both mindfulness and physical activity are shown to improve mood, reduce stress and are widely used as part of a multi-component treatment approach, reducing distressing symptoms of mood and affect disorders. The utility of protein level as a biomarker has been controversial in the literature following issues concerning the assessment of peripheral levels as a proxy for central levels. The principal aim of this study was to investigate the gene expression of three neurotrophins BDNF, FGF2 and NGF as potential biomarkers of mood disorder, at an early stage of these disorders, which are now widely recognised as having pathogenesis related to dysregulation in the neuro-immuno-endocrine axis. In addition, the study will explore the effect of both physical activity and mindfulness on neurotrophin expression clarifying the associations between the success of these interventions seen in the literature and their effect on the change of neurotrophin expression. Current literature reports increased levels of BDNF protein both centrally and peripherally following mood disorder treatment and participation in both physical exercise and mindfulness activities. Based on similarity of structure and function amongst the three neurotrophins, this thesis will hypothesize an increase in BDNF and potentially FGF2 and NGF mRNA following participation in the two interventional modules designed to improve wellbeing in clinical and non-clinical communities. METHOD: In this independent measures design, 28 non-clinical volunteers were randomly allocated to an 8 week intervention, comprising digital health tracking modules and participation in an unstructured increase in Physical Activity or Mindfulness program, to assess the effect of these interventions on levels of mRNA expression. RTqPCR was used to compare relative mRNA abundance in peripheral blood at baseline and 8 week time interval. The control group were allocated to a waitlist for the period of the 8 week study, followed by access to the program of their choice. Change in emotional state was measured using the DASS. RESULT: BDNF expression is shown significantly increased (p 0.01, n=5) in the Physical Activity group, and significantly decreased in the Mindfulness group (p 0.01, n=11). FGF2 and waitlisted controls showed no significant change. In the case of NGF no expression was seen in human peripheral blood either before or after the intervention. DASS scores revealed a significant decrease in negative affect in the Mindfulness group p = 0.03. CONCLUSION: This study revealed a significant positive association between physical activity and BDNF mRNA, although no significant reduction in distressing mood symptoms was shown. This was potentially due to the small group size. Mindfulness was significantly associated with decreasing negative affect, despite an unexpected decrease in BDNF mRNA consistent with pathophysiology of depression, likely related to neuro-immunoendocrine axis disturbance as suggested in the published literature. It is suggested decreasing mRNA levels reflect lower numbers of immune activated leucocytes present in the blood following mood improvement, albeit not verified in the study. This study suggests even in a small non-clinical sample there may be potential benefits to well-being by increasing levels of physical activity or becoming mindful, and that BDNF has potential as a biomarker of emotional state.; Masters by Research
- Publisher
- Federation University Australia
- Rights
- Copyright Gina England
- Rights
- Open Access
- Rights
- This metadata is freely available under a CCO license
- Subject
- Neurotrophic biomarker; Physical activity; Mindfulness; Mood disorder
- Full Text
- Thesis Supervisor
- Charchar, Fadi
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