- Title
- CHROMagar COL-APSE : A selective bacterial culture medium for the isolation and differentiation of colistin-resistant gram-negative pathogens
- Creator
- Abdul Momin, Muhd; Bean, David; Hendriksen, Rene; Haenni, Marisa; Phee, Lynette; Wareham, David
- Date
- 2017
- Type
- Text; Journal article
- Identifier
- http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/164143
- Identifier
- vital:12986
- Identifier
-
https://doi.org/10.1099/jmm.0.000602
- Identifier
- ISBN:0022-2615
- Abstract
- Purpose. A selective chromogenic culture medium for the laboratory isolation and differentiation of colistin resistant Acinetobacter, Pseudomonas, Stenotrophomonas and Enterobacteriaceae spp. (CHROMagar COL-APSE) was developed, evaluated and compared to an existing selective bacterial culture medium (SuperPolymyxin). Methodology. The medium was challenged with 84 isolates, including polymyxin B (POL B)-susceptible and-resistant type strains and colistin (COL)-resistant organisms recovered from human and animal samples. Susceptibility to COL and POL B was determined by agar dilution and broth microtitre dilution. The lower limit for the detection of COL-resistant organisms was also calculated for both CHROMagar COL-APSE and SuperPolymyxin media. The ability to isolate and correctly differentiate COL-resistant organisms within mixed cultures was also assessed and compared using both media. Results. Using CHROMagar COL-APSE, Gram-negative pathogens (n=71) with intrinsic (n=8) or acquired COL (n=63) resistance were recovered with 100% specificity down to the lower limit of detection of 101 colony-forming units (c.f.u.). The growth on SuperPolymyxin was similar, but notably weaker for COL-resistant non-fermentative bacteria (Acinetobacter, Pseudomonas and Stenotrophomonas). CHROMagar COL-APSE was also more sensitive in supporting the growth of Enterobacteriaceae with COL resistance associated with the carriage of mcr-1. Conclusion. CHROMagar COL-APSE is a sensitive and specific medium for the growth of COL-resistant bacterial pathogens. Due to the low limit of detection (101 c.f.u.), it may be useful as a primary isolation medium in the surveillance and recovery of COL-resistant bacteria from complex human, veterinary and environmental samples, especially those with plasmidmediated MCR-1 or novel mechanisms of polymyxin resistance. © 2017 The Authors.
- Publisher
- Microbiology Society
- Relation
- Journal of Medical Microbiology Vol. 66, no. 11 (2017), p. 1554-1561
- Rights
- Copyright © 2017 The Authors.
- Rights
- This metadata is freely available under a CCO license
- Subject
- 06 Biological Sciences; 07 Agricultural and Veterinary Sciences; 11 Medical and Health Sciences; CHROMagar COL-APSE; Colistin; MCR-1; Resistance
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