Specific humoral response of hosts with variable schistosomiasis susceptibility
- Driguez, Patrick, McWilliam, Hamish, Gaze, Soraya, Piedrafita, David, Pearson, Mark, Nakajima, Rie, Duke, Mary, Trieu, Angela, Doolan, Denise, Cardoso, Fernanda, Jasinskas, Algis, Gobert, Geoffrey, Felgner, Philip, Loukas, Alex, Meeusen, Els, McManus, Donald
- Authors: Driguez, Patrick , McWilliam, Hamish , Gaze, Soraya , Piedrafita, David , Pearson, Mark , Nakajima, Rie , Duke, Mary , Trieu, Angela , Doolan, Denise , Cardoso, Fernanda , Jasinskas, Algis , Gobert, Geoffrey , Felgner, Philip , Loukas, Alex , Meeusen, Els , McManus, Donald
- Date: 2016
- Type: Text , Journal article
- Relation: Immunology and Cell Biology Vol. 94, no. 1 (2016), p. 52-65
- Full Text:
- Reviewed:
- Description: The schistosome blood flukes are some of the largest global causes of parasitic morbidity. Further study of the specific antibody response during schistosomiasis may yield the vaccines and diagnostics needed to combat this disease. Therefore, for the purposes of antigen discovery, sera and antibody-secreting cell (ASC) probes from semi-permissive rats and sera from susceptible mice were used to screen a schistosome protein microarray. Following Schistosoma japonicum infection, rats had reduced pathology, increased antibody responses and broader antigen recognition profiles compared with mice. With successive infections, rat global serological reactivity and the number of recognized antigens increased. The local antibody response in rat skin and lung, measured with ASC probes, increased after parasite migration and contributed antigen-specific antibodies to the multivalent serological response. In addition, the temporal variation of anti-parasite serum antibodies after infection and reinfection followed patterns that appear related to the antigen driving the response. Among the 29 antigens differentially recognized by the infected hosts were numerous known vaccine candidates, drug targets and several S. japonicum homologs of human schistosomiasis resistance markers - the tegument allergen-like proteins. From this set, we prioritized eight proteins that may prove to be novel schistosome vaccine and diagnostic antigens. © 2016 Australasian Society for Immunology Inc. All rights reserved.
- Authors: Driguez, Patrick , McWilliam, Hamish , Gaze, Soraya , Piedrafita, David , Pearson, Mark , Nakajima, Rie , Duke, Mary , Trieu, Angela , Doolan, Denise , Cardoso, Fernanda , Jasinskas, Algis , Gobert, Geoffrey , Felgner, Philip , Loukas, Alex , Meeusen, Els , McManus, Donald
- Date: 2016
- Type: Text , Journal article
- Relation: Immunology and Cell Biology Vol. 94, no. 1 (2016), p. 52-65
- Full Text:
- Reviewed:
- Description: The schistosome blood flukes are some of the largest global causes of parasitic morbidity. Further study of the specific antibody response during schistosomiasis may yield the vaccines and diagnostics needed to combat this disease. Therefore, for the purposes of antigen discovery, sera and antibody-secreting cell (ASC) probes from semi-permissive rats and sera from susceptible mice were used to screen a schistosome protein microarray. Following Schistosoma japonicum infection, rats had reduced pathology, increased antibody responses and broader antigen recognition profiles compared with mice. With successive infections, rat global serological reactivity and the number of recognized antigens increased. The local antibody response in rat skin and lung, measured with ASC probes, increased after parasite migration and contributed antigen-specific antibodies to the multivalent serological response. In addition, the temporal variation of anti-parasite serum antibodies after infection and reinfection followed patterns that appear related to the antigen driving the response. Among the 29 antigens differentially recognized by the infected hosts were numerous known vaccine candidates, drug targets and several S. japonicum homologs of human schistosomiasis resistance markers - the tegument allergen-like proteins. From this set, we prioritized eight proteins that may prove to be novel schistosome vaccine and diagnostic antigens. © 2016 Australasian Society for Immunology Inc. All rights reserved.
Current status for gastrointestinal nematode diagnosis in small ruminants: where are we and where are we going?
- Preston, Sarah, Sandeman, Mark, Gonzalez, Jorge, Piedrafita, David
- Authors: Preston, Sarah , Sandeman, Mark , Gonzalez, Jorge , Piedrafita, David
- Date: 2014
- Type: Text , Journal article
- Relation: Journal of immunology research Vol. 2014, no. September (2014), p. Article no. 210350
- Full Text:
- Reviewed:
- Description: Gastrointestinal nematode (GIN) parasites pose a significant economic burden particularly in small ruminant production systems. Anthelmintic resistance is a serious concern to the effective control of GIN parasites and has fuelled the focus to design and promote sustainable control of practices of parasite control. Many facets of sustainable GIN parasite control programs rely on the ability to diagnose infection both qualitatively and quantitatively. Diagnostics are required to determine anthelmintic efficacies, for targeted treatment programs and selection of animals for parasite resistant breeding. This review describes much of the research investigated to date to improve the current diagnostic for the above practices which is based on counting the number of parasite eggs in faeces.
- Authors: Preston, Sarah , Sandeman, Mark , Gonzalez, Jorge , Piedrafita, David
- Date: 2014
- Type: Text , Journal article
- Relation: Journal of immunology research Vol. 2014, no. September (2014), p. Article no. 210350
- Full Text:
- Reviewed:
- Description: Gastrointestinal nematode (GIN) parasites pose a significant economic burden particularly in small ruminant production systems. Anthelmintic resistance is a serious concern to the effective control of GIN parasites and has fuelled the focus to design and promote sustainable control of practices of parasite control. Many facets of sustainable GIN parasite control programs rely on the ability to diagnose infection both qualitatively and quantitatively. Diagnostics are required to determine anthelmintic efficacies, for targeted treatment programs and selection of animals for parasite resistant breeding. This review describes much of the research investigated to date to improve the current diagnostic for the above practices which is based on counting the number of parasite eggs in faeces.
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