- Title
- The effect of human amnion epithelial cells on lung development and inflammation in preterm lambs exposed to antenatal inflammation
- Creator
- Papagianis, Paris; Ahmadi-Noorbakhsh, Siavash; Lim, Rebecca; Wallace, Euan; Polglase, Graeme; Pillow, J. Jane; Moss, Timothy
- Date
- 2021
- Type
- Text; Journal article
- Identifier
- http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/187086
- Identifier
- vital:17010
- Identifier
-
https://doi.org/10.1371/journal.pone.0253456
- Identifier
- ISBN:1932-6203
- Abstract
- Lung inflammation and impaired alveolarization are hallmarks of bronchopulmonary dysplasia (BPD). We hypothesize that human amnion epithelial cells (hAECs) are anti-inflammatory and reduce lung injury in preterm lambs born after antenatal exposure to inflammation. Pregnant ewes received either intra-amniotic lipopolysaccharide (LPS, from E.coli 055:B5 4mg) or saline (Sal) on day 126 of gestation. Lambs were delivered by cesarean section at 128 d gestation (term ~150 d). Lambs received intravenous hAECs (LPS/hAECs: n = 7 30x10.sup.6 cells) or equivalent volumes of saline (LPS/Sal, n = 10 or Sal/Sal, n = 9) immediately after birth. Respiratory support was gradually de-escalated, aimed at early weaning from mechanical ventilation towards unassisted respiration. Lung tissue was collected 1 week after birth. Lung morphology was assessed and mRNA levels for inflammatory mediators were measured. Respiratory support required by LPS/hAEC lambs was not different to Sal/Sal or LPS/Sal lambs. Lung tissue:airspace ratio was lower in the LPS/Sal compared to Sal/Sal lambs (P<0.05), but not LPS/hAEC lambs. LPS/hAEC lambs tended to have increased septation in their lungs versus LPS/Sal (P = 0.08). Expression of inflammatory cytokines was highest in LPS/hAECs lambs. Postnatal administration of a single dose of hAECs stimulates a pulmonary immune response without changing ventilator requirements in preterm lambs born after intrauterine inflammation.
- Publisher
- San Francisco: Public Library of Science
- Relation
- PloS one Vol. 16, no. 6 (2021), p. e0253456-e0253456
- Rights
- All metadata describing materials held in, or linked to, the repository is freely available under a CC0 licence
- Rights
- https://creativecommons.org/licenses/by/4.0/
- Rights
- Copyright © 2021 Papagianis et al.
- Rights
- Open Access
- Subject
- MD Multidisciplinary; Amnion; Amniotic sac; Biology and Life Sciences; Birth; Care and treatment; Cesarean section; Cytokines; Dysplasia; E coli; Engineering and Technology; Epithelial cells; Epithelium; Ethics; Gestation; Gestational age; Gynecology; Health aspects; Health sciences; Immune response; Inflammation; Injury prevention; Intravenous administration; Lambs; Lipopolysaccharides; Lung morphology; Lungs; Medical research; Medicine and Health Sciences; mRNA; Newborn babies; Obstetrics; Septation; Ventilation; Ventilators; Weaning
- Full Text
- Reviewed
- Funder
- This research was supported by an NHMRC Project Grant (1077769), NHMRC Centre for Research Excellence (1057514), two NHMRC Senior Research Fellowships (JJP; 1077691: TJM 1043294), the Victorian Government's Operational Infrastructure Support Program, and the West Australian Government's Medical and Health Research Infrastructure Fund. Unrestricted equipment and consumable support was provided by Chiesi Farmaceutici S.p.A. (poractant alfa); Fisher & Paykel Healthcare (ventilator circuits); and ICU Medical (arterial monitoring lines).
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