- Title
- Comparison of Cytokine profile between Indonesian thin-tailed and merino sheep during A primary infection with fasciola gigantica
- Creator
- Wiedosari,Ening; Piedrafita, David
- Date
- 2012
- Type
- Text; Journal article
- Identifier
- http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/103368
- Identifier
- vital:10931
- Identifier
- http://ojs.unud.ac.id/index.php/jvet/article/view/2133/1320
- Identifier
- ISSN:1411-8327
- Abstract
- The aim of this study was to investigate the expression of cytokines profiles interferon-? (IFN-?),interleukin-5 (IL-5) and IL-10 in Rasciola gigantica resistant Indonesian thin-tailed (ITT) sheep comparedto susceptible merino sheep infected with Fasciola gigantica. A total of ten ITT and merino sheep wererandomly allocated into infected (n=5) and control (n=5) groups, sheep were infected with 250 viablemetacercariae of F. gigantica. The cytokines were determined by isolated mRNA from hepatic lymph nodeby semi-quantitative RT-PCR (Reverse Trancriptase -Polymerase Chain Reaction). The result showedITT sheep produced significantly higher IL-5 and 10 ( P<0,05 ) than merino sheep, while ITT sheep produced less IFN-? ( P<0,05) than merino sheep at 10 weeks post infection. It could be concluded that merino sheep tend to develop T type 1 cells, while the ITT sheep tend to develop T type 2 cells whicheffectively killed F. gigantica.
- Relation
- Jurnal Veteriner Vol. 13, no. 1 (2012), p.20-25
- Rights
- This work is licensed under a Creative Commons Attribution 4.0 International License. p-ISSN: 1411-8327 e-ISSN: 2477-5665. http://creativecommons.org/licenses/by/4.0/
- Rights
- Open Access
- Rights
- This metadata is freely available under a CCO license
- Subject
- Fasciola gigantica; Indonesian thin-tailed sheep; Merino sheep; IL-5; IL-10
- Full Text
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