- Title
- Modulation of TCR signalling components occurs prior to positive selection and lineage commitment in iNKT cells
- Creator
- Dinh, Xuyen; Stanley, Dragana; Smith, Letitia; Moreau, Morgane; Berzins, Stuart
- Date
- 2021
- Type
- Text; Journal article
- Identifier
- http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/182601
- Identifier
- vital:16138
- Identifier
-
https://doi.org/10.1038/s41598-021-02885-w
- Identifier
- ISBN:2045-2322 (ISSN)
- Abstract
- iNKT cells play a critical role in controlling the strength and character of adaptive and innate immune responses. Their unique functional characteristics are induced by a transcriptional program initiated by positive selection mediated by CD1d expressed by CD4+CD8+ (double positive, DP) thymocytes. Here, using a novel Vα14 TCR transgenic strain bearing greatly expanded numbers of CD24hiCD44loNKT cells, we examined transcriptional events in four immature thymic iNKT cell subsets. A transcriptional regulatory network approach identified transcriptional changes in proximal components of the TCR signalling cascade in DP NKT cells. Subsequently, positive and negative selection, and lineage commitment, occurred at the transition from DP NKT to CD4 NKT. Thus, this study introduces previously unrecognised steps in early NKT cell development, and separates the events associated with modulation of the T cell signalling cascade prior to changes associated with positive selection and lineage commitment. © 2021, The Author(s). **Please note that there are multiple authors for this article therefore only the name of the first 5 including Federation University Australia affiliate “Stuart Berzins” is provided in this record**
- Publisher
- Nature Research
- Relation
- Scientific Reports Vol. 11, no. 1 (2021), p.
- Rights
- All metadata describing materials held in, or linked to, the repository is freely available under a CC0 licence
- Rights
- http://creativecommons.org/licenses/by/4.0/
- Rights
- Copyright © The Author(s) 2021
- Rights
- Open Access
- Subject
- MD Multidisciplinary
- Full Text
- Reviewed
- Funder
- XTD was supported by an Australian Development Scholarship. MAJ was supported by an MS Research Australia/NHMRC Research Betty Cuthbert Fellowship. AGB was supported by an Australian National Health and Medical Research Council (NHMRC) Research Fellowship. This project was funded by the NHMRC and intramural funding from James Cook University.
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