- Title
- Evaluation of serological diagnostic tests for typhoid fever in Papua New Guinea using a composite reference standard
- Creator
- Siba, Valentine; Horwood, Paul; Vanuga, Kilagi; Wapling, Johanna; Sehuko, Rebecca; Siba, Peter; Greenhill, Andrew
- Date
- 2012
- Type
- Text; Journal article
- Identifier
- http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/53459
- Identifier
- vital:6146
- Identifier
-
https://doi.org/10.1128/CVI.00380-12
- Identifier
- ISSN:1833 - 1837
- Abstract
- Typhoid fever remains a major global health problem. A major impediment to improving outcomes is the lack of appropriate diagnostic tools, which have not significantly improved in low-income settings for 100 years. We evaluated two commercially available rapid diagnostic tests (Tubex and TyphiDot), a prototype (TyphiRapid TR-02), and the commonly used single-serum Widal test in a previously reported high-burden area of Papua New Guinea. Samples were collected from 530 outpatients with axillary temperatures of ≥37.5°C, and analysis was conducted on all malaria-negative samples (n = 500). A composite reference standard of blood culture and PCR was used, by which 47 participants (9.4%) were considered typhoid fever positive. The sensitivity and specificity of the Tubex (51.1% and 88.3%, respectively) and TyphiDot (70.0% and 80.1%, respectively) tests were not high enough to warrant their ongoing use in this setting; however, the sensitivity and specificity for the TR-02 prototype were promising (89.4% and 85.0%, respectively). An axillary temperature of ≥38.5°C correlated with typhoid fever (P = 0.014). With an appropriate diagnostic test, conducting typhoid fever diagnosis only on patients with high-grade fever could dramatically decrease the costs associated with diagnosis while having no detrimental impact on the ability to accurately diagnose the illness.
- Relation
- Clinical and Vaccine Immunology Vol. 19, no. 11 (2012), p.
- Rights
- Copyright American Society for Microbiology
- Rights
- This metadata is freely available under a CCO license
- Subject
- 1107 Immunology
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