- Title
- Tuneable hybrid hydrogels via complementary self-assembly of a bioactive peptide with a robust polysaccharide
- Creator
- Firipis, Kate; Boyd-Moss, Mitchell; Long, Benjamin; Dekiwadia, Chaitali; Hoskin, William
- Date
- 2021
- Type
- Text; Journal article
- Identifier
- http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/178815
- Identifier
- vital:15466
- Identifier
-
https://doi.org/10.1021/acsbiomaterials.1c00675
- Identifier
- ISBN:2373-9878 (ISSN)
- Abstract
- Synthetic materials designed for improved biomimicry of the extracellular matrix must contain fibrous, bioactive, and mechanical cues. Self-assembly of low molecular weight gelator (LMWG) peptides Fmoc-DIKVAV (Fmoc-aspartic acid-isoleucine-lysine-valine-alanine-valine) and Fmoc-FRGDF (Fmoc-phenylalanine-arginine-glycine-aspartic acid-phenylalanine) creates fibrous and bioactive hydrogels. Polysaccharides such as agarose are biocompatible, degradable, and non-toxic. Agarose and these Fmoc-peptides have both demonstrated efficacy in vitro and in vivo. These materials have complementary properties; agarose has known mechanics in the physiological range but is inert and would benefit from bioactive and topographical cues found in the fibrous, protein-rich extracellular matrix. Fmoc-DIKVAV and Fmoc-FRGDF are synthetic self-assembling peptides that present bioactive cues "IKVAV"and "RGD"designed from the ECM proteins laminin and fibronectin. The work presented here demonstrates that the addition of agarose to Fmoc-DIKVAV and Fmoc-FRGDF results in physical characteristics that are dependent on agarose concentration. The networks are peptide-dominated at low agarose concentrations, and agarose-dominated at high agarose concentrations, resulting in distinct changes in structural morphology. Interestingly, at mid-range agarose concentration, a hybrid network is formed with structural similarities to both peptide and agarose systems, demonstrating reinforced mechanical properties. Bioactive-LMWG polysaccharide hydrogels demonstrate controllable microenvironmental properties, providing the ability for tissue-specific biomaterial design for tissue engineering and 3D cell culture. © 2021 American Chemical Society.
- Publisher
- American Chemical Society
- Relation
- ACS Biomaterials Science and Engineering Vol. 7, no. 7 (2021), p. 3340-3350
- Rights
- All metadata describing materials held in, or linked to, the repository is freely available under a CC0 licence
- Rights
- Copyright © 2021 American Chemical Society
- Subject
- 0903 Biomedical Engineering; Agarose; Biomaterials; Biomimicry; Self-assembling peptide; Tunable scaffolds
- Reviewed
- Funder
- The authors also acknowledge the support of the St. Vincent’s Hospital, Melbourne Research Endowment Fund. K.F. is supported by an RMIT Research Stipend, an RMIT Engineering Scholarship and an Australian Government Research Training Program Scholarship. D.R.N. was supported by a NHMRC Dementia Research Leadership Fellowship (GNT1135687). This research was undertaken on the small-angle X-ray scattering beamline at the Australian Synchrotron, part of ANSTO (application AS182/SAXS/13541).
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