Consecutive bouts of diverse contractile activity alter acute responses in human skeletal muscle

- Coffey, V. G., Pilegaard, H., Garnham, A. P., O'Brien, Brendan, Hawley, J. A.

Title
Consecutive bouts of diverse contractile activity alter acute responses in human skeletal muscle
Creator
Coffey, V. G.; Pilegaard, H.; Garnham, A. P.; O'Brien, Brendan; Hawley, J. A.
Date
2009
Type
Text; Journal article
Identifier
http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/45029
Identifier
vital:1915
Identifier
https://doi.org/10.1152/japplphysiol.91221.2008
Identifier
ISSN:8750-7587
Abstract
We examined acute molecular responses in skeletal muscle to divergent exercise stimuli by combining consecutive bouts of resistance and endurance exercise. Eight men [22.9 ± 6.3 yr, body mass of 73.2 ± 4.5 kg, peak O2 uptake (V̇O2peak) of 54.0 ± 5.7 ml·kg-1·min-1] were randomly assigned to complete trials consisting of either resistance exercise (8 x 5 leg extension, 80% 1 repetition maximum) followed by a bout of endurance exercise (30 min cycling, 70% V̇O2peak) or vice versa. Muscle biopsies were obtained from the vastus lateralis at rest, 15 min after each exercise bout, and after 3 h of passive recovery to determine early signaling and mRNA responses. Phosphorylation of Akt and Akt1Ser473 were elevated 15 min after resistance exercise compared with cycling, with the greatest increase observed when resistance exercise followed cycling (∼55%; P < 0.01). TSC2-mTOR-S6 kinase phosphorylation 15 min after each bout of exercise was similar regardless of the exercise mode. The cumulative effect of combined exercise resulted in disparate mRNA responses. IGF-I mRNA content was reduced when cycling preceded resistance exercise (-42%), whereas muscle ring finger mRNA was elevated when cycling was undertaken after resistance exercise (∼52%; P < 0.05). The hexokinase II mRNA level was higher after resistance cycling (∼45%; P < 0.05) than after cycling-resistance exercise, whereas modest increases in peroxisome proliferator-activated receptor gamma coactivator-1α mRNA did not reveal an order effect. We conclude that acute responses to diverse bouts of contractile activity are modified by the exercise order. Moreover, undertaking divergent exercise in close proximity influences the acute molecular profile and likely exacerbates acute "interference." Copyright © 2009 the American Physiological Society.
Relation
Journal of Applied Physiology Vol. 106, no. 4 (2009), p. 1187-1197
Rights
Copyright American Physiological Society
Rights
Open Access
Rights
This metadata is freely available under a CCO license
Subject
Acute responses; Concurrent training; Endurance
Full Text
Reviewed
  • Hits: 1128
  • Visitors: 1501
  • Downloads: 430
Thumbnail File Description Size Format
SOURCE1 Published Version 514 KB Adobe Acrobat PDF