- Title
- The screening and morphological quantification of bacterial production of calcium carbonate
- Creator
- Seifan, Mostafa; Samani, Ali Khajeh; Berenjian, Aydin; Eckersley, Joshua
- Date
- 2016
- Type
- Text; Conference paper
- Identifier
- http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/162512
- Identifier
- vital:12679
- Abstract
- In recent years, the microbial production of calcium carbonate has emerged as a promising way to address the structural issues associated with concrete structures. Although calcium carbonate can be produced by some genera of bacteria through autotrophic and heterotrophic pathways, the significance level of nutritional and energy sources has remained a challenge to determine. In this study, a Placket-Burman design was used to screen significant factors on the production of calcium carbonate by fermentation; the experimental data was analyzed using Analysis of variance (ANOVA) to determine the significance of each factor. To find the optimum level of nutrients and operating conditions, different media were prepared by altering the concentrations of seven variable factors including inoculum volume, nutrients and calcium sources as well as three constant operating conditions (incubation time, agitation and temperature). Since calcium carbonate polymorphs (calcite, vaterite and aragonite) have different mechanical properties, a morphological quantification using X-ray diffraction (XRD) was developed. It was concluded that peptone and calcium lactate have a negative effect while, remaining variables including urea, yeast extract, and calcium chloride have a positive effect on the production of calcium carbonate by Bacillus sphaericus.
- Relation
- Chemeca 2016 p. 12-22
- Rights
- This metadata is freely available under a CCO license
- Subject
- 0914 Resources Engineering and Extractive Metallurgy; Bacteria; Bacillus sphaericus; Calcium carbonate; Concrete; Morphological quantification; Screening
- Reviewed
- Hits: 1006
- Visitors: 942
- Downloads: 0
| Thumbnail | File | Description | Size | Format |
|---|