- Title
- Xenopus LAP2β protein knockdown affects location of lamin B and nucleoporins and has effect on assembly of cell nucleus and cell viability
- Creator
- Dubińska-Magiera, M.; Chmielewska, M.; Kozioł, K.; Machowska, M.; Hutchison, C. J.; Goldberg, M. W.; Rzepecki, R.
- Date
- 2016
- Type
- Text; Journal article
- Identifier
- http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/170828
- Identifier
- vital:14207
- Identifier
-
https://doi.org/10.1007/s00709-015-0861-y
- Identifier
- ISBN:0033183X (ISSN)
- Abstract
- Xenopus LAP2β protein is the single isoform expressed in XTC cells. The protein localizes on heterochromatin clusters both at the nuclear envelope and inside a cell nucleus. The majority of XLAP2β fraction neither colocalizes with TPX2 protein during interphase nor can be immunoprecipitated with XLAP2β antibody. Knockdown of the XLAP2β protein expression in XTC cells by synthetic siRNA and plasmid encoded siRNA resulted in nuclear abnormalities including changes in shape of nuclei, abnormal chromatin structure, loss of nuclear envelope, mislocalization of integral membrane proteins of INM such as lamin B2, mislocalization of nucleoporins, and cell death. Based on timing of cell death, we suggest mechanism associated with nucleus reassembly or with entry into mitosis. This confirms that Xenopus LAP2 protein is essential for the maintenance of cell nucleus integrity and the process of its reassembly after mitosis. © 2015, The Author(s).
- Publisher
- Springer-Verlag Wien
- Relation
- Protoplasma Vol. 253, no. 3 (2016), p. 943-956
- Rights
- This metadata is freely available under a CCO license
- Subject
- Cell nucleus; Knockdown; Lamin B; LAP2; Nucleoporin; TPX2; Xenopus laevis; Cell cycle protein; DNA binding protein; Lamina-associated polypeptide 2; Membrane protein; Microtubule associated protein; Nuclear protein; Phosphoprotein; Small interfering RNA; TPX2 protein, Xenopus; Xenopus protein; Animal; Cell line; Cell survival; Gene silencing; Genetics; Interphase; Metabolism; mitosis; Ultrastructure; Animals; Cell Cycle Proteins; DNA-Binding Proteins; Gene Knockdown Techniques; Lamin Type B; Membrane Proteins; Microtubule-Associated Proteins; Nuclear Pore Complex Proteins; Nuclear Proteins; Phosphoproteins; RNA, Small Interfering; Xenopus Proteins
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