- Title
- The effect of different adjuvants on immune parameters and protection following vaccination of sheep with a larval-specific antigen of the gastrointestinal nematode, Haemonchus contortus
- Creator
- Piedrafita, David; Preston, Sarah; Kemp, Joanna; De Veer, Michael; Sherrard, Jayne; Kraska, Troy; Elhay, Martin; Meeusen, Els
- Date
- 2013
- Type
- Text; Journal article
- Identifier
- http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/70380
- Identifier
- vital:6548
- Identifier
-
https://doi.org/10.1371/journal.pone.0078357
- Identifier
- ISSN:19326203
- Abstract
- It has recently been recognised that vaccine adjuvants play a critical role in directing the nature of a vaccine induced effector response. In the present study, several adjuvants were evaluated for their ability to protect sheep after field vaccination with the larval-specific Haemonchus contortus antigen, HcsL3. Using a suboptimal antigen dose, aluminium adjuvant was shown to reduce the cumulative faecal egg counts (cFEC) and worm burden by 23% and 25% respectively, in agreement with a previous study. The addition of Quil A to the aluminium-adjuvanted vaccine brought cFEC back to control levels. Vaccination with the adjuvant DEAE-dextran almost doubled the protection compared to the aluminium-adjuvanted vaccine resulting in 40% and 41% reduction in cFEC and worm counts compared to controls. Examination of skin responses following i.d. injection of exsheathed L3, revealed that cFEC was negatively correlated with wheal size and tissue eosinophils for the DEAE-dextran and aluminium-adjuvanted groups respectively. These studies have for the first time shown the potential of DEAE-dextran adjuvant for helminth vaccines, and discovered significant cellular correlates of vaccine-induced protection. Funding: Australian Research Council Linkage grant LP0668945 and the ARC-Centre of Excellence in Structural and Functional Microbial Genomics. The funders have no role in study design, data collection and analysis, decision to publish, or preparation of the manuscrip
- Relation
- PLoS ONE. Vol. 8, no. 10, (Art. no.e78357) (2013), p. 1-8
- Rights
- Copyright 2013 Piedrafita et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- Rights
- Open Access
- Rights
- This metadata is freely available under a CCO license
- Subject
- Md Multidisciplinary
- Full Text
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