Changes in knee joint biomechanics following balance and technique training and a season of Australian football
- Authors: Donnelly, Cyril , Elliot, Bruce , Doyle, Tim , Finch, Caroline , Dempsey, Alasdair , Lloyd, David
- Date: 2012
- Type: Text , Journal article
- Relation: British Journal of Sports Medicine Vol. 46, no. 13 (October 2012 2012), p. 917-922
- Full Text: false
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- Description: Purpose: Determine if balance and technique training (BTT) implemented adjunct to normal Australian football (AF) training reduces external knee loading during sidestepping. Additionally, the authors determined if an athlete's knee joint kinematics and kinetics change over a season of AF. Methodology: Eight amateur-level AF clubs (n=1,001 males) volunteered to participate in either 28 weeks of BTT or a ‘sham’ training (ST) adjunct to their normal preseason and regular training. A subset of 34 athletes (BTT, n=20; ST, n=14) were recruited for biomechanical testing in weeks 1–7 and 18–25 of the 28-week training intervention. During biomechanical testing, participants completed a series running, preplanned (PpSS) and unplanned sidestepping (UnSS) tasks. A linear mixed model (a=0.05) was used to determine if knee kinematics and peak moments during PpSS and UnSS were influenced by BTT and/or a season of AF Results: Both training groups significantly (p=0.025) decreased their peak internal-rotation knee moments during PpSS, and significantly (p=0.022) increased their peak valgus knee moments during UnSS following their respective training interventions. Conclusions: BTT was not effective in changing an athlete's knee joint biomechanics during sidestepping when conducted in ‘real-world’ training environments. Following normal AF training, the players had different changes to their knee joint biomechanics during both preplanned and unplanned sidestepping. When performing an unplanned sidestepping task in the latter half of a playing season, athletes are at an increased risk of ACL injury. The authors therefore recommend both sidestepping tasks are performed during biomechanical testing when assessing the effectiveness of prophylactic training protocols.
Compliance with return-to-play regulations following concussion in Australian schoolboy and community rugby union players
- Authors: Hollis, Stephanie , Stevenson, Mark , McIntosh, Andrew , Shores, E Arthur , Finch, Caroline
- Date: 2012
- Type: Text , Journal article
- Relation: British Journal of Sports Medicine Vol. 46, no. 10 (2012), p. 735-740
- Full Text: false
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- Description: Background: There is a risk of concussion when playing rugby union. Appropriate management of concussion includes compliance with the return-to-play regulations of the sports body for reducing the likelihood of premature return-to-play by injured players. Purpose: To describe the proportion of rugby union players who comply with the sports body's regulations on returning to play postconcussion. Study design: Prospective cohort study. Methods: 1958 community rugby union players (aged 15–48 years) in Sydney (Australia) were recruited from schoolboy, grade and suburban competitions and followed over ≥1 playing seasons. Club doctors/physiotherapists/coaches or trained injury recorders who attended the game reported players who sustained a concussion. Concussed players were followed up over a 3-month period and the dates when they returned to play (including either a game or training session) were recorded, as well as any return-to-play advice they received. Results: 187 players sustained ≥1 concussion throughout the follow-up. The median number of days before players returned to play (competition game play or training) following concussion was 3 (range 1–84). Most players (78%) did not receive return-to-play advice postconcussion, and of those who received correct advice, all failed to comply with the 3-week stand-down regulation. Conclusions: The paucity of return-to-play advice received by community rugby union players postconcussion and the high level of non-compliance with return-to-play regulations highlight the need for better dissemination and implementation of the return-to-play regulations and improved understanding of the underlying causes of why players do not adhere to return-to-play practices.
Do parents' and children's concerns about sports safety and injury risk relate to how much physical activity children do?
- Authors: Telford, Amanda , Finch, Caroline , Barnett, Lisa , Abbott, Gavin , Salmon, Jo
- Date: 2012
- Type: Text , Journal article
- Relation: British Journal of Sports Medicine Vol. 46, no. 15 (2012), p. 1084-1088
- Relation: http://purl.org/au-research/grants/nhmrc/565900
- Full Text: false
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- Description: Objective: To see whether concerns about injury risk relate to children's physical activity (PA). Methods: Two cohorts were recruited from 19 Australian schools and assessed in 2001 (T1), 2004 (T2) and 2006 (T3). The younger (n=162) was assessed at 6, 9 and 11years old, and the older (n=259) at 11, 14 and 16 years old. At T1 and T2, parents of the younger cohort reported on fear of child being injured, and whether child would be at risk of injury if they played organised sport; the older cohort self-reported injury fear. Accelerometers assessed PA at each time point. Linear regression models examined cross-sectional associations, and also associations between T1 injury fear and risk and T2 PA, and T2 injury fear and risk and T3 PA. Results: In the younger cohort at T2 (9 years), fear and risk were both negatively associated with moderate to vigorous PA (MVPA) (β=−0.17, 95% CI −0.30 to −0.03 and β=−0.26, 95% CI −0.41 to −0.10) and also vigorous PA (VPA). Fear was also associated with moderate PA (MPA). For the older cohort at T1, injury fear was negatively associated with MVPA (β=−0.21, 95% CI −0.35 to −0.07) and also MPA and VPA. Parental perception of risk at T1 (6 years) was negatively associated with children’s MPA at T2 (9 years) (β= −0.17, 95% CI −0.32 to −0.02). Sex did not moderate any association. Conclusions Younger children and their parents need to know which sports have low injury risks. Some children may need increased confidence to participate.
- Description: Objective: To see whether concerns about injury risk relate to children's physical activity (PA). Methods: Two cohorts were recruited from 19 Australian schools and assessed in 2001 (T1), 2004 (T2) and 2006 (T3). The younger (n=162) was assessed at 6, 9 and 11years old, and the older (n=259) at 11, 14 and 16 years old. At T1 and T2, parents of the younger cohort reported on fear of child being injured, and whether child would be at risk of injury if they played organised sport; the older cohort self-reported injury fear. Accelerometers assessed PA at each time point. Linear regression models examined cross-sectional associations, and also associations between T1 injury fear and risk and T2 PA, and T2 injury fear and risk and T3 PA. Results: In the younger cohort at T2 (9 years), fear and risk were both negatively associated with moderate to vigorous PA (MVPA) (
Do retirement villages enhance social connectedness?
- Authors: Blume, Suzanne , Macgill, Jennifer
- Date: 2012
- Type: Text , Conference paper
- Relation: Australian Association of Gerontology, 45th National Conference, Australasian Journal on Ageing Vol. 31, p. 6-6
- Full Text: false
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- Description: C1
- Description: In Australia, there is a growing trend toward early retirement at 50 plus years. Many early retirees also make lifestyle changes and move into cluster housing ‘retirement villages’. Some retirement villages offer a range of care services to support inhabitants as they age. The cluster housing concept is designed to enhance security, social collectiveness and connection for older people. This research will look at the relationship of social connectedness and retirement village design through exploration of theoretical concepts and analysis of inhabitants’ stories about life and living in a regional retirement village in Victoria, Australia.
Effect of comorbidity on relative survival following hospitalisation for fall-related hip fracture in older people
- Authors: Hindmarsh, Diane , Loh, Ming , Finch, Caroline , Hayen, Andrew , Close, Jacqueline
- Date: 2012
- Type: Text , Journal article
- Relation: Australasian Journal on Ageing Vol. 33, no. 3 (2012), p. E1-E7
- Relation: http://purl.org/au-research/grants/nhmrc/565900
- Full Text: false
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- Description: To assess the effect of comorbidity on relative survival after hip fracture.
Fetal sex affects expression of renin-angiotensin system components in term human decidua
- Authors: Wang, Yu , Pringle, Kirsty , Sykes, Shane , Marques, Francine , Morris, Brian , Zakar, Tamas , Lumbers, Eugenie
- Date: 2012
- Type: Text , Journal article
- Relation: Endocrinology Vol. 153, no. 1 (January 2012), p. 462-468
- Full Text: false
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- Description: The maternal decidua expresses the genes of the renin-angiotensin system (RAS). Human decidua was collected at term either before labor (i.e. cesarean delivery) or after spontaneous labor. The mRNA for prorenin (REN), prorenin receptor (ATP6AP2), angiotensinogen (AGT), angiotensinconverting enzymes 1 and 2 (ACE1 and ACE2), angiotensin II type 1 receptor (AGTR1), and angiotensin 1-7 receptor (MAS1) were measured by quantitative real-time RT-PCR. Decidual explants were cultured in duplicate for 24 and 48 h, and all RAS mRNA, and the secretion of prorenin, angiotensin II, and angiotensin 1-7 was measured using quantitative real-time RT-PCR, ELISA, and radioimmunoassay, respectively. In the decidua collected before labor, REN mRNA levels were higher if the fetus was female. In addition, REN, ATP6AP2, AGT, and MAS1 mRNA abundance was greater in decidual explants collected from women carrying a female fetus, as was prorenin protein. After 24 h, ACE1 mRNA was higher in the decidual explants from women with a male fetus, whereas after 48 h, both ACE1 and ACE2 mRNA was higher in decidual explants from women with a female fetus. Angiotensin II was present in all explants, but angiotensin 1-7 levels often registered below the lower limits of sensitivity for the assay. After labor, decidua, when compared with nonlaboring decidua, demonstrated lower REN expression when the fetus was female. Therefore, the maternal decidual RAS is regulated in a sex-specific manner, suggesting that it may function differently when the fetus is male than when it is female.
- Description: C1
Field vaccination of sheep with a larval-specific antigen of the gastrointestinal nematode, Haemonchus contortus, confers significant protection against an experimental challenge infection
- Authors: Piedrafita, David , De Veer, Michael , Lydall, Jayne , Kraska, Troy , Elhay, Martin , Meeusen, Els
- Date: 2012
- Type: Text , Journal article
- Relation: Vaccine Vol. 30, no. 50 (2012), p. 7199-7204
- Full Text: false
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- Description: The availability of effective vaccines would add a valuable tool to the management of gastrointestinal nematode infections in livestock. While some experimental vaccines have shown protection in laboratory trials, few have been tested in the field. In the present study, eight month old sheep kept on pasture were treated with anthelmintic 8 weeks before vaccination with a larval surface antigen of the nematode parasite, Haemonchus contortus, under a commercially acceptable protocol, i.e. 2 immunizations using a commercial adjuvant; they were then given a controlled challenge infection 4 weeks later in indoor pens. Vaccination of sheep with 4 increasing doses of antigen resulted in significant reductions of 61% and 27% in cumulative faecal egg counts in the two highest dose groups, and a 69% reduction in worm burden in the highest dose group. Blood loss, as determined by packed cell volume, was also significantly reduced in the highest dose group of sheep. One outlier sheep showed an unusual increase in egg count without a concomitant increase in worm burden compared to the control sheep, indicating a vaccine-induced stress response. Antigen-specific serum antibody levels steadily increased in sheep while on pasture and decreased when transported to indoor pens. No difference in antibody levels could be detected between vaccinated and unvaccinated sheep, but all showed increased antibody levels compared to uninfected control sheep kept in indoors pens for 2–3 months, suggesting sheep were sensitized to the larval antigen either from low dose pasture contamination or cross reaction with pasture-related antigens. The results of these studies confirm the protective properties of the larval surface antigen and its protective effect when vaccinations are performed in the field.
Getting sports injury prevention on to public health agendas – addressing the shortfalls in current information sources
- Authors: Finch, Caroline
- Date: 2012
- Type: Text , Journal article
- Relation: British Journal of Sports Medicine Vol. 46, no. 1 (January 2012 2012), p. 70-74
- Full Text: false
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- Description: Public health policy is a successful population-level strategy for injury prevention but it is yet to be widely applied to the sports sector. Such policy is generally coordinated by government health departments concerned with the allocation of limited resources to health service delivery and preventive programs for addressing large community health issues. Prioritisation of sports injury prevention (SIP) requires high-quality evidence about the size of the problem and its public health burden; identification of at-risk vulnerable groups; confirmed effective prevention solutions; evidence of intervention cost-effectiveness; and quantifi cation of both financial and policy implications of inaction. This paper argues that the major reason for a lack of sports injury policy by government departments for health or sport to date is a lack of relevant information available for policy makers to make their decisions. Key information gaps evident in Australia are used to highlight this problem. SIP policy does not yet rank highly because, relative to other health/injury issues, there is very little hard evidence to support: claims for its priority ranking, the existence of solutions that can be implemented and which will work, and potential cost-savings to government agencies. Moreover, policy action needs to be integrated across government portfolios, including sport, health and others. Until sports medicine research generates high-quality population-level information of direct relevance and importance to policy makers, especially intervention costing and implementation cost-benefit estimates, and fully engage in policy-informing partnerships, SIP will continue to be left off the public health agenda.
Health belief dualism in the postnatal practices of rural Swazi women : An ethnographic account
- Authors: Thwala, Siphiwe , Holroyd, Eleanor , Jones, Linda
- Date: 2012
- Type: Text , Journal article
- Relation: Women and Birth Vol. 25, no. 4 (2012), p. e68-e74
- Full Text: false
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- Description: Objective: This study explores and describes the values, beliefs, and practices of rural Swazi women regarding childbearing in the postpartum period. Method: A retrospective ethnographic research design was used. A snowballing sampling method was used to recruit fifteen participants. Face-to-face unstructured audio-taped interviews and field notes were utilised to gather data. Findings: Results showed that rural Swazi women held a dual health belief system of modern and traditional medicinal use; practiced lengthy periods of postpartum confinement; customarily gave regular enemas and traditional medicines to their babies; undertook the specific cultural practice of taking the baby to enyonini [a tree struck by lightening] to perform specific rituals; used self-prescribed pharmacy medicines; used both traditional and modern contraception; as well as practiced breastfeeding. Conclusion: Rural Swazi women observe modern health practices alongside traditional customary practices that are inherent to their health belief and value systems in the postnatal period. These customary beliefs and values underpin their birth practices postpartum. Recommendations include the need to consider including formal knowledge on cultural aspects of childbirth and postpartum care into midwifery education; a review of maternal care practices and policies to incorporate widely practised traditional elements including redressing the use of self-prescribed pharmacy medicines to ensure a higher level of safety. © 2011 Australian College of Midwives.
Identifying context-specific competencies required by community Australian Football sports trainers
- Authors: Donaldson, Alex , Finch, Caroline
- Date: 2012
- Type: Text , Journal article
- Relation: British Journal of Sports Medicine Vol. 46, no. 10 (August 2012 2012), p. 759-765
- Relation: http://purl.org/au-research/grants/nhmrc/565900
- Relation: http://purl.org/au-research/grants/nhmrc/565907
- Full Text: false
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- Description: Background: First-aid is a recommended injury prevention and risk management strategy in community sport; however, little is known about the sport-specific competencies required by first-aid providers. Objective: To achieve expert consensus on the competencies required by community Australian Football (community-AF) sports trainers. Study design: A three-round online Delphi process. Setting: Community-AF. Participants: 16 Australian sports first-aid and community-AF experts. Outcome measures: Rating of competencies as either ‘essential’, ‘expected’, ‘ideal’ or ‘not required’. Results: After Round 3, 47 of the 77 (61%) competencies were endorsed as ‘essential’ or ‘expected’ for a sports trainer to effectively perform the activities required to the standards expected at a community-AF club by ≥75% of experts. These competencies covered: the role of the sports trainer; the responsibilities of the sports trainer; emergency management; injury and illness assessment and immediate management; taping; and injury prevention and risk management. Four competencies (5%) were endorsed as ‘ideal’ or ‘not required’ by ≥85% of experts and were excluded from further consideration. The 26 competencies where consensus was not reached were retained as second-tier, optional competencies. Conclusions: Sports trainers are important members of on-field first-aid teams, providing support to both injured players and other sports medicine professionals. The competencies identified in this study provide the basis of a proposed two-tiered community-AF–specific sports trainer education structure that can be implemented by the peak sports body. This includes six mandatory modules, relating to the ‘required’ competencies, and a further six optional modules covering competencies on which consensus was not reached.
International medical graduates in Australia : a historical perspective (1930-1950s)
- Authors: Terry, Daniel , Woodroffe, Jessica , Le, Quynh , Ogden, Kathryn
- Date: 2012
- Type: Text , Journal article , Editorial Material
- Relation: Medical Journal of Australia Vol. 197, no. 2 (Jul 2012), p. 84-86
- Full Text: false
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Is there a role for rare variants in DRD4 gene in the susceptibility for ADHD Searching for an effect of allelic heterogeneity
- Authors: Tovo-Rodrigues, Luciana , Rohde, Luis , Roman, Tatiana , Schmitz, Marcelo , Polanczyk, Guilherme , Zeni, Cristian , Marques, Francine , Contini, Veronica , Grevet, Eugenio , Belmonte-De-Abreu, Paulo , Bau, Claiton , Hutz, Mara
- Date: 2012
- Type: Text , Journal article
- Relation: Molecular Psychiatry Vol. 17, no. 5 (May 2012), p. 520-526
- Full Text: false
- Reviewed:
- Description: Although several studies have demonstrated an association between the 7-repeat (7R) allele in the 48-bp variable number of tandem repeats (VNTRs) in the exon 3 at dopamine receptor D4 (DRD4) gene and attention-deficit/ hyperactivity disorder (ADHD), others failed to replicate this finding. In this study, a total of 786 individuals with ADHD were genotyped for DRD4 exon 3 VNTR. All 7R homozygous subjects were selected for VNTR re-sequencing. Subjects homozygous for the 4R allele were selected paired by age, ancestry and disorder subtypes in order to have a sample as homogeneous as possible with 7R/7R individuals. Using these criteria, 103 individuals (66 with ADHD and 37 control individuals) were further investigated. An excess of rare variants were observed in the 7R alleles of ADHD patient when compared with controls (P = 0.031). This difference was not observed in 4R allele. Furthermore, nucleotide changes that predict synonymous and non-synonymous substitutions were more common in the 7R sample (P = 0.008 for total substitutions and P = 0.043 for non-synonymous substitutions). In silico prediction of structural/functional alterations caused by these variants have also been observed. Our findings suggest that not only repeat length but also DNA sequence should be assessed to better understand the role of DRD4 exon 3 VNTR in ADHD genetic susceptibility.
- Description: C1
Looking beyond the obvious: Intra expertise differences are harder to see!
- Authors: Berry, Jason , Carlon, Todd , Young, Warren
- Date: 2012
- Type: Text , Conference paper
- Relation: Journal of Sport & Exercise Psychology Vol. 34, p. S68-S68
- Full Text: false
- Reviewed:
- Description: Sport expertise research provides a robust body of knowledge on the characteristics that separate experts from those less skilled. Perceptual skill is recognized as an important factor in agility performance in team sports like Australian Football (AF). However, perceptual-agility research to date has concentrated on inter expertise skill differences (i.e., elite vs. novice). This study investigated the presence of any intra-group differences within a cohort of elite AF athletes on a simulated perceptual-agility task. Specific aims were to 1) identify if AF athletes predetermined as high agility displayed superior perceptual-agility skill compared to low agility AF athletes, and 2) identify if high experience AF athletes displayed superior perceptual-agility skill compared to low experience AF athletes. Fourteen AF athletes performed a video-based Perceptual-Agility Test (PAT) that assessed the athletes’ decision time and decision accuracy in response to intercepting an attacking player on the projected simulation. Part 1 of the analysis; the athletes were divided into two groups (n = 7 each) by way of median split according to their in-game defensive agility performance scores. T-tests were conducted to reveal any differences between the groups in decision time and decision accuracy (Part 1 and Part 2). No significant differences were found in decision time or decision accuracy between the high and low agility AF athletes. Part 2 of the analysis; the 14 athletes (high experience) were compared to a group of low experience athletes (n = 8) on their PAT performance. No significant differences were found in decision time or decision accuracy between the high and low experience AF athletes. While there were no observable differences within the cohort of elite AF athletes using the predetermined classifications of agility and experience, this study does, however, confirm the difficulty of revealing intra expertise performance indicators using assessment tools that routinely discriminate a priori levels of skill (i.e., expert vs. novice).
Planning for implementation and translation : seek first to understand the end-users’ perspectives
- Authors: Donaldson, Alex , Finch, Caroline
- Date: 2012
- Type: Text , Journal article
- Relation: British Journal of Sports Medicine Vol. 46, no. 5 (April 2012), p. 306-307
- Relation: http://purl.org/au-research/grants/nhmrc/565900
- Full Text: false
- Reviewed:
- Description: Translating research evidence into sustained improvements in population health outcomes is a current priority across many health research fields including sports medicine,1 injury prevention2 and physical activity promotion.3 Both the ‘traditional’ approach of publishing sports injury research findings in peer-reviewed journals and hoping for the best, and the more sophisticated approach of developing and disseminating consensus statements and related sport safety guidelines have been relatively unsuccessful to date.
Predictors of acute bacterial meningitis in children from a malaria-endemic area of Papua New Guinea
- Authors: Laman, Moses , Manning, Laurens , Greenhill, Andrew , Mare, Trevor , Michael, Audrey , Shem, Silas , Vince, John , Lagani, William , Hwaihwanje, Ilomo , Siba, Peter , Mueller, Ivo , Davis, Timothy
- Date: 2012
- Type: Text , Journal article
- Relation: American Journal of Tropical Medicine and Hygiene Vol. 86, no. 2 (2012), p. 240-245
- Full Text: false
- Reviewed:
- Description: Predictors of acute bacterial meningitis (ABM) were assessed in 554 children in Papua New Guinea 0.2-10 years of age who were hospitalized with culture-proven meningitis, probable meningitis, or non-meningitic illness investigated by lumbar puncture. Forty-seven (8.5%) had proven meningitis and 36 (6.5%) had probable meningitis. Neck stiffness, Kernig's and Brudzinski's signs and, in children < 18 months of age, a bulging fontanel had positive likelihood ratios (LRs) ≥ 4.3 for proven/probable ABM. Multiple seizures and deep coma were less predictive (LR = 1.5-2.1). Single seizures and malaria parasitemia had low LRs (≤ 0.5). In logistic regression including clinical variables, Kernig's sign and deep coma were positively associated with ABM, and a single seizure was negatively associated (P ≤ 0.01). In models including microscopy, neck stiffness and deep coma were positively associated with ABM and parasitemia was negatively associated with ABM (P ≤ 0.04). In young children, a bulging fontanel added to the model (P < 0.001). Simple clinical features predict ABM in children in Papua New Guinea but malaria microscopy augments diagnostic precision.
Sport as a setting for promoting health
- Authors: Donaldson, Alex , Finch, Caroline
- Date: 2012
- Type: Text , Journal article
- Relation: British Journal of Sports Medicine Vol. 46, no. 1 (January 2012 2012), p. 4-5
- Full Text: false
- Reviewed:
The NF-κB1 transcription factor prevents the intrathymic development of CD8 T cells with memory properties
- Authors: Gugasyan, Raffi , Horat, Elisha , Kinkel, Sarah , Ross, Fiona , Grigoriadis, George , Gray, Daniel , O'Keeffe, Meredith , Berzins, Stuart , Belz, Gabrielle , Grumont, Raelene , Banerjee, Ashish , Strasser, Andreas , Godfrey, Dale , Tsichlis, Phillip , Gerondakis, Steve
- Date: 2012
- Type: Text , Journal article
- Relation: EMBO Journal Vol. 31, no. 3 (2012), p. 692-706
- Full Text: false
- Reviewed:
- Description: The role of specific members of the NF-κB family of transcription factors in CD8 T-cell selection and development is largely unknown. Here, we show that mice lacking NF-κB1 develop a unique population of conventional CD8 single-positive (SP) thymocytes with memory T cell-like properties that populate peripheral immune organs. Development of this memory-like population is not due to PLZF + thymocytes and instead coincides with changes in CD8 T-cell selection. These include a reduction in the efficiency of negative selection and a dependence on MHC class Ia or Ib expressed by haematopoietic cells. These findings indicate that NF-κB1 regulates multiple events in the thymus that collectively inhibit the excess development of CD8 + thymocytes with memory cell characteristics. © 2012 European Molecular Biology Organization | All Rights Reserved.
A roadmap to generate renewable protein binders to the human proteome
- Authors: Colwill, Karen , Persson, Helena , Jarvik, Nicholas , Wyrzucki, Arkadiusz , Wojcik, John , Koide, Akiko , Kossiakoff, Anthony , Koide, Shohei , Sidhu, Sachdev , Dyson, Michael , Pershad, Kritika , Pavlovic, John , Karatt-Vellatt, Aneesh , Schofield, Darren , Kay, Brian , McCafferty, John , Mersmann, Michael , Meier, Doris , Mersmann, Jana , Helmsing, Saskia , Hust, Michael , Dubel, Stefan , Berkowicz, Susan , Freemantle, Alexia , Spiegel, Michael , Sawyer, Alan , Layton, Daniel , Nice, Edouard , Dai, Anna , Rocks, Oliver , Williton, Kelly , Fellouse, Frederic , Hersi, Kadija , Pawson, Tony , Nilsson, Peter , Sundberg, Marten , Sjoberg, Ronald , Sivertsson, Asa , Schwenk, Jochen , Takanen, Jenny , Hober, Sophia , Uhlen, Mathias , Dahlgren, Lars-Goran , Flores, Alex , Johansson, Ida , Weigelt, Johan , Crombet, Lissette , Loppnau, Peter , Kozieradzki, Ivona , Cossar, Doug , Arrowsmith, C. , Edwards, Aled , Graslund, Susanne
- Date: 2011
- Type: Text , Journal article
- Relation: Nature Methods Vol. 8, no. 7 (2011), p. 551-558
- Full Text: false
- Reviewed:
- Description: Despite the wealth of commercially available antibodies to human proteins, research is often hindered by their inconsistent validation, their poor performance and the inadequate coverage of the proteome. These issues could be addressed by systematic, genome-wide efforts to generate and validate renewable protein binders. We report a multicenter study to assess the potential of hybridoma and phage-display technologies in a coordinated large-scale antibody generation and validation effort. We produced over 1,000 antibodies targeting 20 SH2 domain proteins and evaluated them for potency and specificity by enzyme-linked immunosorbent assay (ELISA), protein microarray and surface plasmon resonance (SPR). We also tested selected antibodies in immunoprecipitation, immunoblotting and immunofluorescence assays. Our results show that high-affinity, high-specificity renewable antibodies generated by different technologies can be produced quickly and efficiently. We believe that this work serves as a foundation and template for future larger-scale studies to create renewable protein binders.
ADRA2A polymorphisms and ADHD in adults : Possible mediating effect of personality
- Authors: de Cerqueira, Caio , Polina, Evelise , Contini, Veronica , Marques, Francine , Grevet, Eugenio , Salgado, Carlos , da Silva, Paula , Picon, Felipe , Belmonte-De-Abreu, Paulo , Bau, Claiton
- Date: 2011
- Type: Text , Journal article
- Relation: Psychiatry Research Vol. 186, no. 2-3 (2011), p. 345-350
- Full Text: false
- Reviewed:
- Description: Several studies have tested for the association between polymorphisms in the ADRA2A gene and childhood ADHD. A meta-analysis of these results, however, has pointed towards a significant heterogeneity, raising the need for explanatory studies. As the effect of other relevant clinical characteristics could be a possible source, we studied three polymorphisms in the ADRA2A gene (−1291 C>G–MspI or rs1800544; −262 G>A–HhaI or rs1800544; 1780 C>T–DraI or rs553668) in 403 adult patients with ADHD assessed in relation to comorbidity and personality characteristics, as well as in 232 controls. The diagnosis followed DSM-IV criteria, and personality dimensions were evaluated with the Temperament and Character Inventory (TCI). There were no significant differences in allele and genotype frequencies between cases and controls. Patients carrying the G allele of rs1800544 presented lower scores in harm avoidance, and carriers of the T allele of rs553668 had more novelty seeking and less harm avoidance and persistence. Additionally, the haplotype carrying the G-G-T alleles (rs1800544–rs1800545–rs553668) was associated with lower scores in harm avoidance and persistence, and higher scores in novelty seeking compared to other haplotypes. These findings suggest that the conflicting findings obtained in association studies between ADRA2A polymorphisms and ADHD might be related to temperament profiles, and support additional studies addressing these effects in larger samples.
Attitude and behaviour of junior rugby union players towards tackling during training and match play
- Authors: Finch, Caroline
- Date: 2011
- Type: Text , Journal article
- Relation: Safety Science Vol. 50, no. 4 (April 2012), p. 1157
- Full Text: false
- Reviewed: