Reducing climate change related fugitive greenhouse gas emissions from operational longwall coal mines
- Authors: Holmes, Robert
- Date: 2017
- Type: Text , Thesis , PhD
- Full Text:
- Description: The aim of this research is to quantify and validate a method which can significantly reduce fugitive greenhouse gas emissions from collieries in Australia, both cost-effectively and safely. Methane (CH₄) is controlled in collieries currently only for safety, statutory compliance or for capture and use reasons. But today, there is pressure on collieries to reduce not only mining costs but their greenhouse gas emissions. It is known that 65% of greenhouse gas (GHG) emissions associated with collieries come from fugitive ventilation air methane (VAM). The oxidising machinery to mitigate these fugitive emissions is expensive, has safety concerns and is not widely used at present for these reasons. But widespread concern over GHG emissions means that it is desirable to lower VAM emissions now. One safe, low-cost and non-gas drainage solution explored herein to reduce emissions, is a method to prevent some CH₄ from entering the mine airstream and becoming VAM in the first place. This emissions reduction method underwent a 12-month trial in a colliery in the Hunter Valley using six different quantified and costed non-gas drainage measures. All relevant data was retained, and with the mine’s permission has been processed and published here as a part of this research. A reduction in fugitive emissions of 95,398 t/CO₂-e below that projected for the subsequent 12 months was quantified, at a mitigation cost of A$1.08 t/CO₂-e. The level of mitigation achieved, represents approximately 20% of the mine’s VAM emissions. This research has also further tested the method used in the Hunter Valley trial, by visiting two other large collieries in Queensland, and assessing the two most successful mitigation measures from the Hunter Valley trial (roadway sealing and pressure balancing of sealed panels) against operational conditions at these collieries by ventilation modelling, using their measured gas, airflow and seal pressure data.
- Description: Doctor of Philosophy
- Authors: Holmes, Robert
- Date: 2017
- Type: Text , Thesis , PhD
- Full Text:
- Description: The aim of this research is to quantify and validate a method which can significantly reduce fugitive greenhouse gas emissions from collieries in Australia, both cost-effectively and safely. Methane (CH₄) is controlled in collieries currently only for safety, statutory compliance or for capture and use reasons. But today, there is pressure on collieries to reduce not only mining costs but their greenhouse gas emissions. It is known that 65% of greenhouse gas (GHG) emissions associated with collieries come from fugitive ventilation air methane (VAM). The oxidising machinery to mitigate these fugitive emissions is expensive, has safety concerns and is not widely used at present for these reasons. But widespread concern over GHG emissions means that it is desirable to lower VAM emissions now. One safe, low-cost and non-gas drainage solution explored herein to reduce emissions, is a method to prevent some CH₄ from entering the mine airstream and becoming VAM in the first place. This emissions reduction method underwent a 12-month trial in a colliery in the Hunter Valley using six different quantified and costed non-gas drainage measures. All relevant data was retained, and with the mine’s permission has been processed and published here as a part of this research. A reduction in fugitive emissions of 95,398 t/CO₂-e below that projected for the subsequent 12 months was quantified, at a mitigation cost of A$1.08 t/CO₂-e. The level of mitigation achieved, represents approximately 20% of the mine’s VAM emissions. This research has also further tested the method used in the Hunter Valley trial, by visiting two other large collieries in Queensland, and assessing the two most successful mitigation measures from the Hunter Valley trial (roadway sealing and pressure balancing of sealed panels) against operational conditions at these collieries by ventilation modelling, using their measured gas, airflow and seal pressure data.
- Description: Doctor of Philosophy
Reflective leadership : A self-study of practice
- Authors: Ludvigsen, Donna
- Date: 2017
- Type: Text , Thesis , PhD
- Full Text:
- Description: Encouraging reflective leadership practice is a recognised focus for coaches and consultants in primary schools. Despite the wealth of knowledge on coaching and consulting in organisational learning, there is less known about the transition between these two interactions and the purpose behind this. This self-study of my practice as a regional school improvement officer in Victoria, Australia, details my interaction with six teacher-leaders, who considered school improvement strategies by constructing a pattern-block representation of the structures within their schools. The research questions posed were: “What do teacher-leaders reflect upon when considering school improvement approaches?” and “How does the external facilitator encourage teacherleaders to reflect upon their practice?” Research undertaken for this study was used to develop a range of statements related to Donald Schön’s theory of reflective practice, particularly reflection-in-action. The statements were presented as a stimulus for action in practice and encompassed a range of tensions. Tensions included; orchestrating change by moving between the current situation and a preferred future; making connections by acknowledging the complexity of the situation whilst attempting to create order; developing a strategy from the consideration of a shared vision and a written plan; and undertaking leadership by moving between hierarchical, distributive and self-organising structures. Tacit and explicit knowledge was evident when reflecting-inaction. Finally I explored how, as the external facilitator, I encouraged reflection by moving between coaching and consulting interactions. I began this research by examining the practice of others. However, the greatest learning I achieved related to how I reflected-in-action and my understanding of self-study in practice. Initially in this study, I placed myself at the centre of the research and considered this a selfin- study; however, as the study progressed, I examined both my practice and the research simultaneously, terming this self-and-study, and, finally, from my analysis of the research when the study concluded, I developed a series of statements that could be used as a stimulus for future action, which I refer to as self-from-study. The larger implication for this research therefore relates to my understanding of my own reflection-in-action and how by embracing a number of tensions one can consider a range of possibilities for improvement.
- Description: Doctor of Philosophy
- Authors: Ludvigsen, Donna
- Date: 2017
- Type: Text , Thesis , PhD
- Full Text:
- Description: Encouraging reflective leadership practice is a recognised focus for coaches and consultants in primary schools. Despite the wealth of knowledge on coaching and consulting in organisational learning, there is less known about the transition between these two interactions and the purpose behind this. This self-study of my practice as a regional school improvement officer in Victoria, Australia, details my interaction with six teacher-leaders, who considered school improvement strategies by constructing a pattern-block representation of the structures within their schools. The research questions posed were: “What do teacher-leaders reflect upon when considering school improvement approaches?” and “How does the external facilitator encourage teacherleaders to reflect upon their practice?” Research undertaken for this study was used to develop a range of statements related to Donald Schön’s theory of reflective practice, particularly reflection-in-action. The statements were presented as a stimulus for action in practice and encompassed a range of tensions. Tensions included; orchestrating change by moving between the current situation and a preferred future; making connections by acknowledging the complexity of the situation whilst attempting to create order; developing a strategy from the consideration of a shared vision and a written plan; and undertaking leadership by moving between hierarchical, distributive and self-organising structures. Tacit and explicit knowledge was evident when reflecting-inaction. Finally I explored how, as the external facilitator, I encouraged reflection by moving between coaching and consulting interactions. I began this research by examining the practice of others. However, the greatest learning I achieved related to how I reflected-in-action and my understanding of self-study in practice. Initially in this study, I placed myself at the centre of the research and considered this a selfin- study; however, as the study progressed, I examined both my practice and the research simultaneously, terming this self-and-study, and, finally, from my analysis of the research when the study concluded, I developed a series of statements that could be used as a stimulus for future action, which I refer to as self-from-study. The larger implication for this research therefore relates to my understanding of my own reflection-in-action and how by embracing a number of tensions one can consider a range of possibilities for improvement.
- Description: Doctor of Philosophy
The assumed divide : Examining gender, feminism and relationships through visual art
- Authors: Hollis, Sylvia
- Date: 2017
- Type: Text , Thesis , Masters
- Full Text: false
- Description: The Assumed Divide is a visual arts based, practice-led research project which explores expectations of gender within the context of interpersonal relationships, structured through feminist theory. I created a collection of small, figurative sculptures inspired by experiences of animosity and misunderstanding, to locate areas of gendered interpersonal ambivalence. This thesis proposes that feminism might enhance gender relations by deconstructing harmful stereotypes and by encouraging empathy and respect for diversity. Issues such as reproductive choice, intergenerational debate, and uncertainty in aims are discussed as apparent impediments to the unity of feminism, against a patriarchal tradition that grants men categorical universality. I argue that by dismantling the perception of male unification and by elucidating the multitudinous similarities and variances of human experience, feminism makes advances in eliminating sexism. I also examine how binary gender division, with an assumption of distinct difference between male and female, generates conflict and power dynamics advantageous to men, diminishing the quality of heterosexual and social relationships. The observation that overlapping gender traits blur boundaries of male and female, and that rigid categorisations are not indisputably representative of all people, may offer more points of connection to bridge the divide of gender. Each artwork in The Assumed Divide reflects on gendered experience, with sculpted depictions of disintegrated flesh acting as visual manifestations of the psyches and social conditions of the figures. Working realistically on a small scale, and integrating found silver trays as motifs of domesticity and relationships, my intent is to directly stimulate contemplation of the themes in context to viewers’ lives, recognising that the audience is free to find their own meaning in the works. Discussion of these pieces, alongside works by other artists who broach topics of feminism, gender, and relationships, exemplifies the capacity for art to infuse theory with personal insight, inspired and derived from the lives of both artist and viewer.
- Description: Masters by Research
The development of the Malaysian vocational education and training system
- Ramanathan Chettiar, Palaniappan
- Authors: Ramanathan Chettiar, Palaniappan
- Date: 2017
- Type: Text , Thesis , PhD
- Full Text:
- Description: The vocational education and training (VET) sector in Malaysia has experienced significant change over the last 10 years. This thesis aimed to identify the challenges facing VET development in Malaysia and consider the lessons that can be learnt from Australian and Singaporean VET experiences. This research explored the challenges using a mixed methods approach (qualitative and quantitative) to answer the research question from a multiple stakeholder perspective. The 42 respondents included policymakers, chief executive officers (CEOs) from industry, CEOs from registered training organisations (RTOs), VET instructors and trainees. There were 34 respondents from Malaysia involved in the interviews. Two policymakers and two CEOs of RTOs from Singapore and Australia were also involved in the interviews. An online survey was answered by 209 Malaysian respondents. Significantly, the literature review, including literature from Malaysia, Australia, Singapore, the United Kingdom, Europe and the United States, focused on the development of VET policy and the ideas associated with vocational education theory, human capital theory, systems theory and threshold theory, program theory, employability theory, collective skills formation, stakeholder engagement and the capability approach. The literature review provided an overview of the motivations behind government initiates to expand skills development initiatives using different models, the shifting of responsibility for employability to the employee and promoting inclusivity within the nation. While VET objectives were overt, the need for socially inclusive results were not. There is a need to consider economic, social and education contexts when developing VET systems, given the diversity of Malaysian society. The argument is made within the thesis that the development of VET policy has been largely led by government and legislation, rather than industry. The thesis suggests that challenges inherent in the development of a VET system arise from the gaps between VET policy and practice, rhetoric and implementation. While the presence of policy mechanisms for system efficiency was acknowledged by stakeholders, VET policy was characterised by respondents as incremental rather than long term. Most stakeholders acknowledged the importance of lifelong learning and for the need for Malaysia to become a learning nation. The lack of a single agency to monitor skills development was considered an obstacle for effective skills development within the country. Accordingly, this thesis proposes a set of recommendations to address these challenges and provides directions for future research.
- Description: Doctor of Philosophy
- Authors: Ramanathan Chettiar, Palaniappan
- Date: 2017
- Type: Text , Thesis , PhD
- Full Text:
- Description: The vocational education and training (VET) sector in Malaysia has experienced significant change over the last 10 years. This thesis aimed to identify the challenges facing VET development in Malaysia and consider the lessons that can be learnt from Australian and Singaporean VET experiences. This research explored the challenges using a mixed methods approach (qualitative and quantitative) to answer the research question from a multiple stakeholder perspective. The 42 respondents included policymakers, chief executive officers (CEOs) from industry, CEOs from registered training organisations (RTOs), VET instructors and trainees. There were 34 respondents from Malaysia involved in the interviews. Two policymakers and two CEOs of RTOs from Singapore and Australia were also involved in the interviews. An online survey was answered by 209 Malaysian respondents. Significantly, the literature review, including literature from Malaysia, Australia, Singapore, the United Kingdom, Europe and the United States, focused on the development of VET policy and the ideas associated with vocational education theory, human capital theory, systems theory and threshold theory, program theory, employability theory, collective skills formation, stakeholder engagement and the capability approach. The literature review provided an overview of the motivations behind government initiates to expand skills development initiatives using different models, the shifting of responsibility for employability to the employee and promoting inclusivity within the nation. While VET objectives were overt, the need for socially inclusive results were not. There is a need to consider economic, social and education contexts when developing VET systems, given the diversity of Malaysian society. The argument is made within the thesis that the development of VET policy has been largely led by government and legislation, rather than industry. The thesis suggests that challenges inherent in the development of a VET system arise from the gaps between VET policy and practice, rhetoric and implementation. While the presence of policy mechanisms for system efficiency was acknowledged by stakeholders, VET policy was characterised by respondents as incremental rather than long term. Most stakeholders acknowledged the importance of lifelong learning and for the need for Malaysia to become a learning nation. The lack of a single agency to monitor skills development was considered an obstacle for effective skills development within the country. Accordingly, this thesis proposes a set of recommendations to address these challenges and provides directions for future research.
- Description: Doctor of Philosophy
The immanence of traumatic rupture : From the extra/ordinary to the originary
- Authors: Pedersen, Cassie
- Date: 2017
- Type: Text , Thesis , PhD
- Full Text:
- Description: This thesis critically intervenes into the interdisciplinary space of trauma theory by both identifying and circumventing the tendency of theorists to posit trauma in a relation of either transcendence or immanence to the contexts in which it occurs. In the classical trauma theories of Shoshana Felman, Dori Laub, and Cathy Caruth, trauma is broadly defined as a disruptive and aporetic event that shatters the cognitive, experiential, and representational frameworks necessary for making sense of the occurrence. These theorists conceptualise trauma as transcendent, seeing trauma as existing “outside” or “beyond” the frameworks in which it comes into being. However, more recent critics enter a polemic with classical trauma theorists by reconceptualising trauma as immanent to the all too human frameworks that facilitate its occurrence in the first place. I contend that the mutual exclusive insistence that trauma need either be conceived as immanent to, or transcendent of, the frameworks in which it occurs has led to a conceptual impasse in trauma theory that is rooted in a false dichotomy between these extremes. Tracing this oppositional tendency across a broad disciplinary spectrum, engaging contributions to trauma theory from philosophy, literary theory, and history, the major aim of this thesis to move beyond the false dichotomy between the immanent and the transcendent by revealing that these terms are inextricably bound. Drawing on the works of Hannah Arendt, Giorgio Agamben, Sigmund Freud, Jacques Lacan, Jean Laplanche, and Jean-François Lyotard (to name only a few), this thesis revitalises the space of trauma theory by offering a series of interlocking arguments that conceptualise the alterity of trauma as being immanent to the frameworks it transcends. This paradoxical logic is at the crux of what I refer to as the immanence of traumatic rupture.
- Description: Doctor of Philosophy
- Authors: Pedersen, Cassie
- Date: 2017
- Type: Text , Thesis , PhD
- Full Text:
- Description: This thesis critically intervenes into the interdisciplinary space of trauma theory by both identifying and circumventing the tendency of theorists to posit trauma in a relation of either transcendence or immanence to the contexts in which it occurs. In the classical trauma theories of Shoshana Felman, Dori Laub, and Cathy Caruth, trauma is broadly defined as a disruptive and aporetic event that shatters the cognitive, experiential, and representational frameworks necessary for making sense of the occurrence. These theorists conceptualise trauma as transcendent, seeing trauma as existing “outside” or “beyond” the frameworks in which it comes into being. However, more recent critics enter a polemic with classical trauma theorists by reconceptualising trauma as immanent to the all too human frameworks that facilitate its occurrence in the first place. I contend that the mutual exclusive insistence that trauma need either be conceived as immanent to, or transcendent of, the frameworks in which it occurs has led to a conceptual impasse in trauma theory that is rooted in a false dichotomy between these extremes. Tracing this oppositional tendency across a broad disciplinary spectrum, engaging contributions to trauma theory from philosophy, literary theory, and history, the major aim of this thesis to move beyond the false dichotomy between the immanent and the transcendent by revealing that these terms are inextricably bound. Drawing on the works of Hannah Arendt, Giorgio Agamben, Sigmund Freud, Jacques Lacan, Jean Laplanche, and Jean-François Lyotard (to name only a few), this thesis revitalises the space of trauma theory by offering a series of interlocking arguments that conceptualise the alterity of trauma as being immanent to the frameworks it transcends. This paradoxical logic is at the crux of what I refer to as the immanence of traumatic rupture.
- Description: Doctor of Philosophy
The lost opportunity of Melbourne's outer circle railway
- Authors: McKenna, Trevor
- Date: 2017
- Type: Text , Thesis , Masters
- Full Text:
- Description: This thesis examines a little known railway line in Melbourne, the Outer Circle Railway (OCR) running from Oakleigh in the south-east to Fairfield in the north. There is great significance to its east-north trajectory, because I will argue, it was conceptualised as a major part of the Melbourne system, for the future; a future that ostensibly lasted only two years, before the economic depression of the 1890s caused it to close in stages. It further brought in many more strands to the Melbourne transport nexus, as it circumnavigated the inner suburbs. I ask the key question ‘was the Outer Circle Railway a lost opportunity’? Though considered one of the great public transport cities of the world, Melbourne with its extensive rail and tramway networks succumbed to the winds of change, embodied by the Fordist principles of private car ownership, freeway building and traffic systems. The public transport budget was cut in the second half of the twentieth century – leaving Greater Melbourne in constant gridlock, in the twenty-first century. I use documentary analysis to examine primary and secondary documents, to comprehend whether the almost immediate denigration of the OCR, by newspapers and most historians, was warranted. Certainly I find many contemporaneous champions of the OCR, which was built to the highest British standard, as was the entire Victorian Railways system. My key finding is that the OCR rail line could be utilised in the modern era. The OCR route is pointed squarely towards Tullamarine airport, and could be used as a basis for the long-sought rail link to the airport. The decision of the Andrews Labor Government in 2014 to abandon the contentious East-West road tunnel in favour of rail projects reflects the need for a growing city to privilege public transport, as it did in the nineteenth century.
- Description: Masters by Research
- Authors: McKenna, Trevor
- Date: 2017
- Type: Text , Thesis , Masters
- Full Text:
- Description: This thesis examines a little known railway line in Melbourne, the Outer Circle Railway (OCR) running from Oakleigh in the south-east to Fairfield in the north. There is great significance to its east-north trajectory, because I will argue, it was conceptualised as a major part of the Melbourne system, for the future; a future that ostensibly lasted only two years, before the economic depression of the 1890s caused it to close in stages. It further brought in many more strands to the Melbourne transport nexus, as it circumnavigated the inner suburbs. I ask the key question ‘was the Outer Circle Railway a lost opportunity’? Though considered one of the great public transport cities of the world, Melbourne with its extensive rail and tramway networks succumbed to the winds of change, embodied by the Fordist principles of private car ownership, freeway building and traffic systems. The public transport budget was cut in the second half of the twentieth century – leaving Greater Melbourne in constant gridlock, in the twenty-first century. I use documentary analysis to examine primary and secondary documents, to comprehend whether the almost immediate denigration of the OCR, by newspapers and most historians, was warranted. Certainly I find many contemporaneous champions of the OCR, which was built to the highest British standard, as was the entire Victorian Railways system. My key finding is that the OCR rail line could be utilised in the modern era. The OCR route is pointed squarely towards Tullamarine airport, and could be used as a basis for the long-sought rail link to the airport. The decision of the Andrews Labor Government in 2014 to abandon the contentious East-West road tunnel in favour of rail projects reflects the need for a growing city to privilege public transport, as it did in the nineteenth century.
- Description: Masters by Research
The Port Phillip Lime Economy : The vessels, the industry and their decline
- Authors: Taylor, Peter
- Date: 2017
- Type: Text , Thesis , Masters
- Full Text:
- Description: The objective of this Master of Arts thesis is to address the previous knowledge gap that existed with regards to the unwritten history of the Port Phillip lime economy. The particular focus of this thesis concerns the participating craft that helped to drive that economy, the types of craft and a number of shipwrecks concerning lime craft.
- Description: Masters by Research
- Authors: Taylor, Peter
- Date: 2017
- Type: Text , Thesis , Masters
- Full Text:
- Description: The objective of this Master of Arts thesis is to address the previous knowledge gap that existed with regards to the unwritten history of the Port Phillip lime economy. The particular focus of this thesis concerns the participating craft that helped to drive that economy, the types of craft and a number of shipwrecks concerning lime craft.
- Description: Masters by Research
The role of Telomeres in the development of Cardiac Hypertrophy
- Authors: Booth, Scott
- Date: 2017
- Type: Text , Thesis , PhD
- Full Text:
- Description: Doctor of Philosophy
- Description: The overall aim of this thesis was to determine the role of telomeres in the development of cardiac hypertrophy. It was hypothesised that changes in cardiomyocyte telomere length and/or maintenance cause cardiac hypertrophy.
- Authors: Booth, Scott
- Date: 2017
- Type: Text , Thesis , PhD
- Full Text:
- Description: Doctor of Philosophy
- Description: The overall aim of this thesis was to determine the role of telomeres in the development of cardiac hypertrophy. It was hypothesised that changes in cardiomyocyte telomere length and/or maintenance cause cardiac hypertrophy.
Theoretical perspectives on using epidemiology and systems thinking to better understand the aetiology and prevention of distance running-related injury
- Authors: Hulme, Adam
- Date: 2017
- Type: Text , Thesis , PhD
- Full Text:
- Description: On a global scale, the sporting activity of distance running has increased in popularity. This is likely attributable to a growing societal concern for the documented rise in several lifestyle-related chronic diseases. As a form of exercise, running provides significant beneficial effects on a range of biomedical health indices, and is the preferred physical activity of choice for many people given its high accessibility and relatively low financial cost. Notwithstanding the many health-related benefits associated with running, the risk of sustaining a distance running-related injury (RRI) can be high. Therefore, from an injury prevention perspective, understanding why runners sustain RRI is of primary scientific importance. Over the last fifty years, the science behind RRI causation and prevention has attracted considerable interest amongst sports injury prevention researchers. During that time, there has been a concerted scholarly effort to understand the aetiology of RRI from an epidemiological and clinical research-based standpoint. Traditional scientific approaches have attempted to identify the effect of discrete trainingrelated, behavioural, and/or biomechanical exposures on the risk of developing either general or specific RRI. Despite what is now a considerable body of work, several descriptive and systematic reviews have found a history of previous injury to be the only definitive risk factor for subsequent RRI development. Alongside the continuing application of traditional scientific approaches, this PhD thesis promotes the use of a complementary ‘systems thinking’ theoretical perspective for better understanding the development and prevention of RRI. There are several contained chapters, the first of which is a systematic review of the RRI aetiological literature. After examining in closer detail the causal mechanism underpinning RRI development, a series of papers urge injury prevention scientists to consider drawing on alternative philosophical perspectives when planning and designing research. In building on the preceding arguments, the final chapters involve the construction of a systems ergonomics control structure model of the Australian distance running system, including the way RRI is managed and controlled.
- Description: Doctor of Philosophy
- Authors: Hulme, Adam
- Date: 2017
- Type: Text , Thesis , PhD
- Full Text:
- Description: On a global scale, the sporting activity of distance running has increased in popularity. This is likely attributable to a growing societal concern for the documented rise in several lifestyle-related chronic diseases. As a form of exercise, running provides significant beneficial effects on a range of biomedical health indices, and is the preferred physical activity of choice for many people given its high accessibility and relatively low financial cost. Notwithstanding the many health-related benefits associated with running, the risk of sustaining a distance running-related injury (RRI) can be high. Therefore, from an injury prevention perspective, understanding why runners sustain RRI is of primary scientific importance. Over the last fifty years, the science behind RRI causation and prevention has attracted considerable interest amongst sports injury prevention researchers. During that time, there has been a concerted scholarly effort to understand the aetiology of RRI from an epidemiological and clinical research-based standpoint. Traditional scientific approaches have attempted to identify the effect of discrete trainingrelated, behavioural, and/or biomechanical exposures on the risk of developing either general or specific RRI. Despite what is now a considerable body of work, several descriptive and systematic reviews have found a history of previous injury to be the only definitive risk factor for subsequent RRI development. Alongside the continuing application of traditional scientific approaches, this PhD thesis promotes the use of a complementary ‘systems thinking’ theoretical perspective for better understanding the development and prevention of RRI. There are several contained chapters, the first of which is a systematic review of the RRI aetiological literature. After examining in closer detail the causal mechanism underpinning RRI development, a series of papers urge injury prevention scientists to consider drawing on alternative philosophical perspectives when planning and designing research. In building on the preceding arguments, the final chapters involve the construction of a systems ergonomics control structure model of the Australian distance running system, including the way RRI is managed and controlled.
- Description: Doctor of Philosophy
Your stories, my stories, our stories : Power/knowledge relations and Koorie perspectives in discourses of Australian History Education
- Authors: Weuffen, Sara
- Date: 2017
- Type: Text , Thesis , PhD
- Full Text:
- Description: Over the past decade, popularised notions and approaches to the teaching and learning of Australia’s history have been overwhelmingly researched and written by non-Indigenous academics. This research challenges dominant non-Indigenous curriculum and research agendas by exploring how, why, and to what degree Koorie, and by extension Aboriginal and Torres Strait Islander perspectives, are taken up for the development and implementation of school-based curriculum aligned to the Year Nine Australian Curriculum: History. The research is guided by Michel Foucault’s poststructural theory to examine a range of discourses identified by year nine history teachers and three Koorie Elders in Ballarat and Greater Shepparton. It is supplemented through Martin Nakata’s ground breaking work on Indigenous Standpoint Theory to acknowledge and highlight the cross-cultural/racial power/knowledge relations of peoples who are involved in the research. It is a timely response to the 2013 mandatory implementation of the Australian Curriculum: History in Victorian state schools. The research builds upon academic research (see Clark, 2006; Harrison & Greenfield, 2011; Mackinlay & Barney, 2011; 2014b) about how teachers may engage critically with Aboriginal and Torres Strait Islander specific content. It contributes significantly to a field of research that has not received much attention over the past eleven years. The research is a striking contribution to understandings of Australian cross-cultural/racial research and education practices. It argues that teachers are not necessarily insensitive to cross-cultural/racial relations operating in Australia; rather, that more rigorous and comprehensive teacher education programs are required for the integration of Koorie perspectives on Australian history. The research clearly demonstrates that stories from local Koorie communities offers up a wealth of knowledge that may be drawn upon to reform curriculum agendas towards shared-history understandings of Australia’s history. Ultimately, it advocates for a more nuanced and mature conversation about contemporary cross-cultural/racial education practices in Australia.
- Description: Doctor of Philosophy
- Authors: Weuffen, Sara
- Date: 2017
- Type: Text , Thesis , PhD
- Full Text:
- Description: Over the past decade, popularised notions and approaches to the teaching and learning of Australia’s history have been overwhelmingly researched and written by non-Indigenous academics. This research challenges dominant non-Indigenous curriculum and research agendas by exploring how, why, and to what degree Koorie, and by extension Aboriginal and Torres Strait Islander perspectives, are taken up for the development and implementation of school-based curriculum aligned to the Year Nine Australian Curriculum: History. The research is guided by Michel Foucault’s poststructural theory to examine a range of discourses identified by year nine history teachers and three Koorie Elders in Ballarat and Greater Shepparton. It is supplemented through Martin Nakata’s ground breaking work on Indigenous Standpoint Theory to acknowledge and highlight the cross-cultural/racial power/knowledge relations of peoples who are involved in the research. It is a timely response to the 2013 mandatory implementation of the Australian Curriculum: History in Victorian state schools. The research builds upon academic research (see Clark, 2006; Harrison & Greenfield, 2011; Mackinlay & Barney, 2011; 2014b) about how teachers may engage critically with Aboriginal and Torres Strait Islander specific content. It contributes significantly to a field of research that has not received much attention over the past eleven years. The research is a striking contribution to understandings of Australian cross-cultural/racial research and education practices. It argues that teachers are not necessarily insensitive to cross-cultural/racial relations operating in Australia; rather, that more rigorous and comprehensive teacher education programs are required for the integration of Koorie perspectives on Australian history. The research clearly demonstrates that stories from local Koorie communities offers up a wealth of knowledge that may be drawn upon to reform curriculum agendas towards shared-history understandings of Australia’s history. Ultimately, it advocates for a more nuanced and mature conversation about contemporary cross-cultural/racial education practices in Australia.
- Description: Doctor of Philosophy
A conceptual re-alignment of methodology underpinning tax effect accounting : An Australian exploration of the contemporary normalising effect
- Authors: Morton, Elizabeth
- Date: 2016
- Type: Text , Thesis , PhD
- Full Text:
- Description: This research examines the presence and effectiveness of the ‘normalising effect’, traditionally offered as the main justification for tax effect accounting’s (TEA) adoption. TEA can be seen as a technical facet of accounting practice, ‘normalising’ the timing differences between the accounting and taxation systems. That is, income tax is recognised according to when transactions are recognised for accounting purposes in order to ‘normalise’ reported profits, thereby reflecting an income statement focus. It has been contended that this will improve the usefulness of financial reports by ‘correcting’ misleading and ‘unreal’ fluctuations in income tax. Australia’s adoption of AIFRS in 2005 entailed a major conceptual re-alignment of the methodology underpinning TEA, moving away from the income statement focus in favour of a balance sheet focus. This implied a different normalisation emphasis. It is within this contemporary setting, based on a study of 90 companies over the two regulatory periods between 2002 and 2011 (AGAAP and AIFRS), that a quantitative measure of the presence and effectiveness of the normalising effect was undertaken, additionally considering the subsequent balance sheet impact. Effective normalisation was revealed during the AGAAP period, whilst only effective after the removal of loss makers during the AIFRS period. These findings suggest that the relaxation of recognition criteria under AIFRS may have had a meaningful impact on the effectiveness of the new standard. However, when normalisation was given a more narrow definition in light of prima facie tax, deferred taxes had a more substantial impact, particularly during the AIFRS period. Such findings are consistent with the notion thatTEA enables reported tax to be ‘as if’ it were a function of accounting, without a substantial build up on the balance sheet as a consequence. These findings have implications for evaluating the efficacy of TEA and comprehending the nature of contemporary financial statements.
- Description: Doctor of Philosophy
- Authors: Morton, Elizabeth
- Date: 2016
- Type: Text , Thesis , PhD
- Full Text:
- Description: This research examines the presence and effectiveness of the ‘normalising effect’, traditionally offered as the main justification for tax effect accounting’s (TEA) adoption. TEA can be seen as a technical facet of accounting practice, ‘normalising’ the timing differences between the accounting and taxation systems. That is, income tax is recognised according to when transactions are recognised for accounting purposes in order to ‘normalise’ reported profits, thereby reflecting an income statement focus. It has been contended that this will improve the usefulness of financial reports by ‘correcting’ misleading and ‘unreal’ fluctuations in income tax. Australia’s adoption of AIFRS in 2005 entailed a major conceptual re-alignment of the methodology underpinning TEA, moving away from the income statement focus in favour of a balance sheet focus. This implied a different normalisation emphasis. It is within this contemporary setting, based on a study of 90 companies over the two regulatory periods between 2002 and 2011 (AGAAP and AIFRS), that a quantitative measure of the presence and effectiveness of the normalising effect was undertaken, additionally considering the subsequent balance sheet impact. Effective normalisation was revealed during the AGAAP period, whilst only effective after the removal of loss makers during the AIFRS period. These findings suggest that the relaxation of recognition criteria under AIFRS may have had a meaningful impact on the effectiveness of the new standard. However, when normalisation was given a more narrow definition in light of prima facie tax, deferred taxes had a more substantial impact, particularly during the AIFRS period. Such findings are consistent with the notion thatTEA enables reported tax to be ‘as if’ it were a function of accounting, without a substantial build up on the balance sheet as a consequence. These findings have implications for evaluating the efficacy of TEA and comprehending the nature of contemporary financial statements.
- Description: Doctor of Philosophy
A hand made wood object : Studio investigation into transformed nature
- Authors: Rein, Jeannette
- Date: 2016
- Type: Text , Thesis , Masters
- Full Text:
- Description: This research explores the importance of retaining traditional hand skills in terms of their relevance to contemporary and future art practice. I examine the hand made and the process of transforming timber into a wooden sculptural form. I investigate how the artist thinks with the material, and how this process gives the artist the ‘sight’ to identify new and original possibilities. Furthermore, I explore how the transformative approach perpetuates new knowledge, and how skills are modified and adapted to suit the changes. I describe this as a dialogic process. The research examines the correlation between the transformative process and the hand made object, the imprint of the maker and how their memories of the processes used, remain embedded in the object. In addition, this research investigates the transformation processes used in creating an object, to provide individualisation within our highly mechanised world, while providing a bridge connecting the past and the future. Through the examination of traditional hand skills, I demonstrate how such skills provide an anchor, a standard of quality and artisanship that connects artists from traditional wood practice, through contemporary praxis, to hand made digital art. My research focuses on the transformation process and traditional hand skills, the vital role they play in the creation of digital hand made objects; as digital processes utilize new materials, processes and machinery that interfaces with traditional analogue tools.
- Description: Masters by Research
- Authors: Rein, Jeannette
- Date: 2016
- Type: Text , Thesis , Masters
- Full Text:
- Description: This research explores the importance of retaining traditional hand skills in terms of their relevance to contemporary and future art practice. I examine the hand made and the process of transforming timber into a wooden sculptural form. I investigate how the artist thinks with the material, and how this process gives the artist the ‘sight’ to identify new and original possibilities. Furthermore, I explore how the transformative approach perpetuates new knowledge, and how skills are modified and adapted to suit the changes. I describe this as a dialogic process. The research examines the correlation between the transformative process and the hand made object, the imprint of the maker and how their memories of the processes used, remain embedded in the object. In addition, this research investigates the transformation processes used in creating an object, to provide individualisation within our highly mechanised world, while providing a bridge connecting the past and the future. Through the examination of traditional hand skills, I demonstrate how such skills provide an anchor, a standard of quality and artisanship that connects artists from traditional wood practice, through contemporary praxis, to hand made digital art. My research focuses on the transformation process and traditional hand skills, the vital role they play in the creation of digital hand made objects; as digital processes utilize new materials, processes and machinery that interfaces with traditional analogue tools.
- Description: Masters by Research
A philosophical analysis of the functions and operation of due process in accounting standard setting in Australia
- Authors: Mehta, Surinder
- Date: 2016
- Type: Text , Thesis , Masters
- Full Text:
- Description: The setting of accounting standards in the Australian context has undergone significant change over the past few decades because of both the introduction of international accounting standards and the outcomes of the Corporate Law Economic Reform Program (CLERP). One major thrust of these reforms was to widen the breadth of participation and to introduce new voices into the Australian accounting standard setting regime. Though there have been recent changes to Australian accounting standard setting arrangements, due process still remains as the underlying procedure for incorporating stakeholder participation. Standard setting resides within a socio-political and economic realm which lies between a capitalistic economic individualism premised upon self-interest and interest group preferences, and an idealistic paradigm of the public interest (common good). Both notions are espoused within modern civil society and present in the formulation of policy and regulation through participation. Recent times have seen the promulgation of accounting standards given over by delegated legislation to private sector interests who compete for desired outcomes and more recently this has been entrenched within the International Accounting Standards Board (IASB) which produces International Financial Reporting Standards (IFRS). This research presents a two-fold investigation of the due process which is applied when developing accounting standards in Australia. Firstly, this study questions whether such standards provide an adequate avenue for incorporating both appropriate attributes of participation and accountability in line with the historical understanding of due process as developed through common law. Secondly, this study questions whether such standards provide an appropriate vehicle for widening participation by introducing new voices into the Australian accounting standard setting process. On the former question, common law due process resonates within a paradigm that supports fair dealing and equity between individuals with themselves and with government. On the latter question, this research identifies low and declining stakeholder participation within the due process of accounting standard setting. This is accompanied by the declining interest of previous major participants. In examining the longitudinal participation trends of submitters to the accounting standard setting regime, this research contends that the due process finds itself embedded within the confines of a procedural construct to the detriment of accommodating natural justice through a more enlightened substantive approach. As such, when considered in light of its common law roots, it fails both to provide an adequate level of openness along with accommodating only a limited opportunity to participate, specifically when taking into account opinions and interests. The very construct of common law due process rests upon its ability both to subsume participation within the decision-making process, and for that participation to be substantive in the outcome of the process. That is, such participation gives meaning to the outcome. Instead the current due process as applied within the accounting standard setting environment resonates such participation through abolitionist type approaches. Here self-interested parties compete for advantages from property rights and private benefits in line with the more modern interpretation of the public interest. The approach taken in this thesis is to consider the due process from a philosophical perspective by assessing the questions of accountability and participation within the due process in terms of civil society, the public good, economic individualism, libertarianism, liberalism, and agency, rather than to embed the discussion within the expansive existing studies concerning due process in the accounting standard setting process. The conclusion from this perspective is that due process is clearly a process past its due date.
- Description: Masters by Research
- Authors: Mehta, Surinder
- Date: 2016
- Type: Text , Thesis , Masters
- Full Text:
- Description: The setting of accounting standards in the Australian context has undergone significant change over the past few decades because of both the introduction of international accounting standards and the outcomes of the Corporate Law Economic Reform Program (CLERP). One major thrust of these reforms was to widen the breadth of participation and to introduce new voices into the Australian accounting standard setting regime. Though there have been recent changes to Australian accounting standard setting arrangements, due process still remains as the underlying procedure for incorporating stakeholder participation. Standard setting resides within a socio-political and economic realm which lies between a capitalistic economic individualism premised upon self-interest and interest group preferences, and an idealistic paradigm of the public interest (common good). Both notions are espoused within modern civil society and present in the formulation of policy and regulation through participation. Recent times have seen the promulgation of accounting standards given over by delegated legislation to private sector interests who compete for desired outcomes and more recently this has been entrenched within the International Accounting Standards Board (IASB) which produces International Financial Reporting Standards (IFRS). This research presents a two-fold investigation of the due process which is applied when developing accounting standards in Australia. Firstly, this study questions whether such standards provide an adequate avenue for incorporating both appropriate attributes of participation and accountability in line with the historical understanding of due process as developed through common law. Secondly, this study questions whether such standards provide an appropriate vehicle for widening participation by introducing new voices into the Australian accounting standard setting process. On the former question, common law due process resonates within a paradigm that supports fair dealing and equity between individuals with themselves and with government. On the latter question, this research identifies low and declining stakeholder participation within the due process of accounting standard setting. This is accompanied by the declining interest of previous major participants. In examining the longitudinal participation trends of submitters to the accounting standard setting regime, this research contends that the due process finds itself embedded within the confines of a procedural construct to the detriment of accommodating natural justice through a more enlightened substantive approach. As such, when considered in light of its common law roots, it fails both to provide an adequate level of openness along with accommodating only a limited opportunity to participate, specifically when taking into account opinions and interests. The very construct of common law due process rests upon its ability both to subsume participation within the decision-making process, and for that participation to be substantive in the outcome of the process. That is, such participation gives meaning to the outcome. Instead the current due process as applied within the accounting standard setting environment resonates such participation through abolitionist type approaches. Here self-interested parties compete for advantages from property rights and private benefits in line with the more modern interpretation of the public interest. The approach taken in this thesis is to consider the due process from a philosophical perspective by assessing the questions of accountability and participation within the due process in terms of civil society, the public good, economic individualism, libertarianism, liberalism, and agency, rather than to embed the discussion within the expansive existing studies concerning due process in the accounting standard setting process. The conclusion from this perspective is that due process is clearly a process past its due date.
- Description: Masters by Research
A three tier forensic model for automatic identification of evidence of child exploitation by analysing the content of chat-logs
- Authors: Miah, Md Waliur Rahman
- Date: 2016
- Type: Text , Thesis , PhD
- Full Text:
- Description: Detection of child exploitation (CE) in Internet chatting by locating evidence in the chat-log is an important issue for the protection of children from prospective online paedophiles. The un-grammatical and informal nature of chat-text makes it difficult for existing formal language processing techniques to handle the problem. The methodology of the current research avoids those difficulties by developing a multi-tier digital forensic model bulit on new ideas of psychological similarity measures and ways of applying them to chat-texts. The model uses text classifiers in the beginning to identify shallow evidence of CE. For locating the particular evidence it is required to identify the behavioural pattern of CE chats consisting of documented CE psychological stages and associate the perpetrators' posts to them. Similarities among the posts of a chat play an important role for the task of differentiating and identifying these stages. To accomplish this task a novel similarity measure is constructed backed by a dictionary with terms associated with each CE stage. Using the new similarity measure is constructed backed by a dictionary with terms associated with each CE stage. Using the new similarity measure in a hieraarchial agglomerative algoritm a new clusterer is built to cluster the posts of a chat-log into the CE stages to learn whether it follows the CE pattern. Inspired by the field of recognition of textual entailment a new soft entailment technique is developed and implemented to locate the specific posts associated with the CE stages. Those specific posts of the perpetrator are extarcted as the particular evidence from the chat-log. It is anticipated that the developed methodology will have many future pratical implementations. It would assist in the development of forensic tools for digital forensic experts in law and enforcement agencies to conveniently locate evidence of online child grooming in a confiscated hard disk drive. Another future implementation would be a parental filter used by parents to protect their children from potential online offenders.
- Description: Doctor of Philosphy
- Authors: Miah, Md Waliur Rahman
- Date: 2016
- Type: Text , Thesis , PhD
- Full Text:
- Description: Detection of child exploitation (CE) in Internet chatting by locating evidence in the chat-log is an important issue for the protection of children from prospective online paedophiles. The un-grammatical and informal nature of chat-text makes it difficult for existing formal language processing techniques to handle the problem. The methodology of the current research avoids those difficulties by developing a multi-tier digital forensic model bulit on new ideas of psychological similarity measures and ways of applying them to chat-texts. The model uses text classifiers in the beginning to identify shallow evidence of CE. For locating the particular evidence it is required to identify the behavioural pattern of CE chats consisting of documented CE psychological stages and associate the perpetrators' posts to them. Similarities among the posts of a chat play an important role for the task of differentiating and identifying these stages. To accomplish this task a novel similarity measure is constructed backed by a dictionary with terms associated with each CE stage. Using the new similarity measure is constructed backed by a dictionary with terms associated with each CE stage. Using the new similarity measure in a hieraarchial agglomerative algoritm a new clusterer is built to cluster the posts of a chat-log into the CE stages to learn whether it follows the CE pattern. Inspired by the field of recognition of textual entailment a new soft entailment technique is developed and implemented to locate the specific posts associated with the CE stages. Those specific posts of the perpetrator are extarcted as the particular evidence from the chat-log. It is anticipated that the developed methodology will have many future pratical implementations. It would assist in the development of forensic tools for digital forensic experts in law and enforcement agencies to conveniently locate evidence of online child grooming in a confiscated hard disk drive. Another future implementation would be a parental filter used by parents to protect their children from potential online offenders.
- Description: Doctor of Philosphy
An investigation into the relationships between novel Y chromosome-linked long non-coding RNAs and coronary artery disease
- Authors: Molina, Elsa
- Date: 2016
- Type: Text , Thesis , PhD
- Full Text:
- Description: Coronary artery disease (CAD) is the most common type of cardiovascular disease and is one of the leading causes of morbidity and mortality globally. However, the pathogenesis of atherosclerosis which leads to CAD and results in heart attacks, heart failure and death is not well understood. In this context, studies have demonstrated a positive correlation between increased hepatic free fatty acids (FFAs) in atherosclerosis and CAD. Although CAD has welldefined environmental risk factors, genome-wide association studies (GWAS) have demonstrated a genetic influence on CAD. Previous studies have shown that genetic variation within the human Y chromosome is associated with an increased risk of developing CAD independent of traditional cardiovascular risk factors; possibly through a modulating effect of an adaptive immunity and inflammatory response by macrophages in men. However, no Y chromosome-linked gene has been investigated in this disease so far. Long non-coding RNAs (lncRNAs) have recently gained focused attention as a new class of regulatory RNA molecules involved in cardiovascular function and associated disease, particularly long intergenic noncoding RNAs (lincRNAs), the largest class within the lncRNA group so far. To date, Y chromosome-linked lincRNAs are poorly characterised and the potential link between these non-coding RNA molecules and CAD in men has not been investigated. In this context, I hypothesised that Y chromosome-linked lncRNAs may regulate pathways involved in lipid metabolism and trigger an over accumulation of FFAs in coronary arteries contributing to atherosclerosis, the underlying cause of CAD. The main objective of this thesis was to therefore further investigate the relationship between the Y chromosome, lncRNAs and CAD in light of the deficiencies within the literature to better understand the causative molecular mechanisms of CAD pathophysiology in men. In my first study (Chapter 2), I identified for the first time through gene expression analysis (real-time PCR) the expression of the following (unannotated in PubMed) Y chromosomelinked lincRNA transcripts: lnc-KDM5D-4:1, lnc-ZFY-1:1, lnc-ZFY-1:3, lnc-ZFY-2:1, lnc- RBMY1B-1:1, lnc-RBMY1B-1:4, lnc-RBMY1J-1:1, lnc-RBMY1J-1:2, and lnc-RBMY1J- 1:3, across 21 different normal, human tissues such as adipose, bladder, brain, cervix, colon, esophagus, heart, kidney, liver, lung, ovary, placenta, prostate, skeletal muscle, small intestine, spleen, testes, thymus, thyroid, trachea, and white blood cells (WBCs) (leukocytes). I found that Y-linked lincRNAs were expressed at low levels (with the lowest CT number equal at 24.5) with a high tissue-specificity for some. Also, the Y-linked RNA gene lnc-KDM5D-4 was widely expressed across male tissues while the Y-linked RNA gene lnc-RBMY1J-1 was specific to the testes. Furthermore, this study presents the first evidence through gene expression analysis that the Y chromosome-linked lincRNA transcripts, lnc-KDM5D-4:1, lnc- ZFY-1:1, lnc-ZFY-1:3, lnc-ZFY-2:1, lnc-RBMY1B-1:1, lnc-RBMY1B-1:4, and lnc- RBMY1J-1:3 are expressed in male leukocytes. Hence, these lincRNAs could be potential non-protein coding gene candidates for CAD research. Knowing that the Y chromosome contributes to lipid levels in humans, to further explore the potential function of these Y-linked lincRNAs in CAD in men, I then studied their expression in a fatty liver context (steatosis-associated atherosclerosis) (Chapter 3). This was performed using the human hepatocellular liver carcinoma cell line, HepG2; the human model of liver cells in CAD research. This study showed for the first time that the Y-linked lincRNA transcripts lnc-KDM5D-4:1, lnc-ZFY-1:1, lnc-ZFY-2:1, lnc-RBMY1B-1:1, and lncxix RBMY1B-1:4 were expressed in HepG2 cells, hence in hepatocellular carcinoma (HCC). Furthermore, this study demonstrated that lnc-KDM5D-4 is a nuclear-retained lincRNA using RNA fluorescence in situ hybridisation (RNA FISH), and is upregulated in palmitate-induced steatosis within hepatocytes (Fold Change = 2.16; p-value = 0.00216). The human Atherosclerosis RT2 Profiler™ PCR Array determined that the silencing of lnc-KDM5D-4 in HepG2 cells was triggering the upregulation of the inhibitor of apoptosis (IAP) gene baculoviral IAP repeat containing 3 (BIRC3) (Fold Change = 12.45, p-value = 0.000025), a well-described protein-coding gene expressed by vascular smooth muscle cells and macrophage foam cells of the inflamed vascular wall of atherosclerotic arteries. Furthermore, perilipin 2 (PLIN2), a gene known to be implicated in lipid metabolism, was also found upregulated. Therefore, this study provides the first evidence for the involvement of a Ychromosome- linked lincRNA, lnc-KDM5D-4, in steatosis-associated atherosclerosis and its retained-nuclear cellular localisation in human hepatocytes, suggesting a function which takes place in the cell nucleus and may play a role in regulating metabolic processes in the liver that are implicated in atherosclerosis. Having shown that a Y chromosome-linked lincRNA could be involved in the determination of lipid level and hence atherosclerosis in men, and to further explore the role of lnc-KDM5D- 4, the expression of this Y-linked lincRNA was studied in human coronary artery smooth muscle cells, especially in atherosclerotic coronary artery cells (Chapter 4). The expression of other non-coding RNAs were also studied such as the protein kinase, Y-linked, pseudogene (PRKY) - previously considered as a new functional candidate for the development of CAD. By analysing the transcriptome of human atherosclerotic and non-atherosclerotic coronary artery smooth muscle cells, I established evidence for the implication of the human Y chromosome in atherosclerosis and CAD. This study exposed the general underexpression of the transcripts from the Y chromosome in atherosclerotic cells implicating a loss or a repression of this chromosome in relation to CAD. Furthermore, this research determined by RNA sequencing a significant downregulation of seven transcripts from Y chromosome genes, including RPS4Y1, USP9Y, DDX3Y, TXLNGY, NLGN4Y and PRKY. RNA FISH determined the subcellular localisation of PRKY in smooth muscle cells by showing a nuclear and a cytoplasmic expression. Furthermore, qPCR gene expression analysis demonstrated that lnc- KDM5D-4 is significantly downregulated in atherosclerotic cells in comparison to the nonatherosclerotic cells. Together, these results showed that lnc-KDM5D-4 is a potential regulator of PLIN2 and BIRC3 genes. Therefore, the downregulation of lnc-KDM5D-4 in atherosclerotic coronary artery smooth muscle cells suggests that this downregulation could be linked to the inflammation of the vascular smooth muscle cells in pathophysiology of CAD via the inhibition of apoptosis of the vascular smooth muscle cells triggered by the upregulation of BIRC3 in these cells. Overall, this study is the first to emphasise a potential involvement of a Y-specific lincRNA, called lnc-KDM5D-4, as a potential contributor to physiology in males. Lnc-KDM5D-4 knockdown resulted in an upregulation of anti-apoptosis and lipid metabolism-related genes. Collectively, our data suggest that the male–specific lnc-KDM5D-4 may regulate key processes in cellular inflammation that trigger atherosclerosis and CAD in men. Accordingly, this data suggests that lnc-KDM5D-4 may provide a novel molecular biomarker for atherosclerotic arteries, and could potentially lead to revolutionary treatment modalities on Y-linked lincRNA as therapeutic agents to manipulate CAD-causing genes in men.
- Description: Doctor of Philosophy
- Authors: Molina, Elsa
- Date: 2016
- Type: Text , Thesis , PhD
- Full Text:
- Description: Coronary artery disease (CAD) is the most common type of cardiovascular disease and is one of the leading causes of morbidity and mortality globally. However, the pathogenesis of atherosclerosis which leads to CAD and results in heart attacks, heart failure and death is not well understood. In this context, studies have demonstrated a positive correlation between increased hepatic free fatty acids (FFAs) in atherosclerosis and CAD. Although CAD has welldefined environmental risk factors, genome-wide association studies (GWAS) have demonstrated a genetic influence on CAD. Previous studies have shown that genetic variation within the human Y chromosome is associated with an increased risk of developing CAD independent of traditional cardiovascular risk factors; possibly through a modulating effect of an adaptive immunity and inflammatory response by macrophages in men. However, no Y chromosome-linked gene has been investigated in this disease so far. Long non-coding RNAs (lncRNAs) have recently gained focused attention as a new class of regulatory RNA molecules involved in cardiovascular function and associated disease, particularly long intergenic noncoding RNAs (lincRNAs), the largest class within the lncRNA group so far. To date, Y chromosome-linked lincRNAs are poorly characterised and the potential link between these non-coding RNA molecules and CAD in men has not been investigated. In this context, I hypothesised that Y chromosome-linked lncRNAs may regulate pathways involved in lipid metabolism and trigger an over accumulation of FFAs in coronary arteries contributing to atherosclerosis, the underlying cause of CAD. The main objective of this thesis was to therefore further investigate the relationship between the Y chromosome, lncRNAs and CAD in light of the deficiencies within the literature to better understand the causative molecular mechanisms of CAD pathophysiology in men. In my first study (Chapter 2), I identified for the first time through gene expression analysis (real-time PCR) the expression of the following (unannotated in PubMed) Y chromosomelinked lincRNA transcripts: lnc-KDM5D-4:1, lnc-ZFY-1:1, lnc-ZFY-1:3, lnc-ZFY-2:1, lnc- RBMY1B-1:1, lnc-RBMY1B-1:4, lnc-RBMY1J-1:1, lnc-RBMY1J-1:2, and lnc-RBMY1J- 1:3, across 21 different normal, human tissues such as adipose, bladder, brain, cervix, colon, esophagus, heart, kidney, liver, lung, ovary, placenta, prostate, skeletal muscle, small intestine, spleen, testes, thymus, thyroid, trachea, and white blood cells (WBCs) (leukocytes). I found that Y-linked lincRNAs were expressed at low levels (with the lowest CT number equal at 24.5) with a high tissue-specificity for some. Also, the Y-linked RNA gene lnc-KDM5D-4 was widely expressed across male tissues while the Y-linked RNA gene lnc-RBMY1J-1 was specific to the testes. Furthermore, this study presents the first evidence through gene expression analysis that the Y chromosome-linked lincRNA transcripts, lnc-KDM5D-4:1, lnc- ZFY-1:1, lnc-ZFY-1:3, lnc-ZFY-2:1, lnc-RBMY1B-1:1, lnc-RBMY1B-1:4, and lnc- RBMY1J-1:3 are expressed in male leukocytes. Hence, these lincRNAs could be potential non-protein coding gene candidates for CAD research. Knowing that the Y chromosome contributes to lipid levels in humans, to further explore the potential function of these Y-linked lincRNAs in CAD in men, I then studied their expression in a fatty liver context (steatosis-associated atherosclerosis) (Chapter 3). This was performed using the human hepatocellular liver carcinoma cell line, HepG2; the human model of liver cells in CAD research. This study showed for the first time that the Y-linked lincRNA transcripts lnc-KDM5D-4:1, lnc-ZFY-1:1, lnc-ZFY-2:1, lnc-RBMY1B-1:1, and lncxix RBMY1B-1:4 were expressed in HepG2 cells, hence in hepatocellular carcinoma (HCC). Furthermore, this study demonstrated that lnc-KDM5D-4 is a nuclear-retained lincRNA using RNA fluorescence in situ hybridisation (RNA FISH), and is upregulated in palmitate-induced steatosis within hepatocytes (Fold Change = 2.16; p-value = 0.00216). The human Atherosclerosis RT2 Profiler™ PCR Array determined that the silencing of lnc-KDM5D-4 in HepG2 cells was triggering the upregulation of the inhibitor of apoptosis (IAP) gene baculoviral IAP repeat containing 3 (BIRC3) (Fold Change = 12.45, p-value = 0.000025), a well-described protein-coding gene expressed by vascular smooth muscle cells and macrophage foam cells of the inflamed vascular wall of atherosclerotic arteries. Furthermore, perilipin 2 (PLIN2), a gene known to be implicated in lipid metabolism, was also found upregulated. Therefore, this study provides the first evidence for the involvement of a Ychromosome- linked lincRNA, lnc-KDM5D-4, in steatosis-associated atherosclerosis and its retained-nuclear cellular localisation in human hepatocytes, suggesting a function which takes place in the cell nucleus and may play a role in regulating metabolic processes in the liver that are implicated in atherosclerosis. Having shown that a Y chromosome-linked lincRNA could be involved in the determination of lipid level and hence atherosclerosis in men, and to further explore the role of lnc-KDM5D- 4, the expression of this Y-linked lincRNA was studied in human coronary artery smooth muscle cells, especially in atherosclerotic coronary artery cells (Chapter 4). The expression of other non-coding RNAs were also studied such as the protein kinase, Y-linked, pseudogene (PRKY) - previously considered as a new functional candidate for the development of CAD. By analysing the transcriptome of human atherosclerotic and non-atherosclerotic coronary artery smooth muscle cells, I established evidence for the implication of the human Y chromosome in atherosclerosis and CAD. This study exposed the general underexpression of the transcripts from the Y chromosome in atherosclerotic cells implicating a loss or a repression of this chromosome in relation to CAD. Furthermore, this research determined by RNA sequencing a significant downregulation of seven transcripts from Y chromosome genes, including RPS4Y1, USP9Y, DDX3Y, TXLNGY, NLGN4Y and PRKY. RNA FISH determined the subcellular localisation of PRKY in smooth muscle cells by showing a nuclear and a cytoplasmic expression. Furthermore, qPCR gene expression analysis demonstrated that lnc- KDM5D-4 is significantly downregulated in atherosclerotic cells in comparison to the nonatherosclerotic cells. Together, these results showed that lnc-KDM5D-4 is a potential regulator of PLIN2 and BIRC3 genes. Therefore, the downregulation of lnc-KDM5D-4 in atherosclerotic coronary artery smooth muscle cells suggests that this downregulation could be linked to the inflammation of the vascular smooth muscle cells in pathophysiology of CAD via the inhibition of apoptosis of the vascular smooth muscle cells triggered by the upregulation of BIRC3 in these cells. Overall, this study is the first to emphasise a potential involvement of a Y-specific lincRNA, called lnc-KDM5D-4, as a potential contributor to physiology in males. Lnc-KDM5D-4 knockdown resulted in an upregulation of anti-apoptosis and lipid metabolism-related genes. Collectively, our data suggest that the male–specific lnc-KDM5D-4 may regulate key processes in cellular inflammation that trigger atherosclerosis and CAD in men. Accordingly, this data suggests that lnc-KDM5D-4 may provide a novel molecular biomarker for atherosclerotic arteries, and could potentially lead to revolutionary treatment modalities on Y-linked lincRNA as therapeutic agents to manipulate CAD-causing genes in men.
- Description: Doctor of Philosophy
Applying prospect-refuge theory to a local environment : My house and the nature strip outside my house
- Authors: Ryan, Vin
- Date: 2016
- Type: Text , Thesis , PhD
- Full Text:
- Description: Jay Appleton’s prospect-refuge theory asserts that there are innate, evolutionary factors that influence how we respond to the landscapes around us. Appleton identifies an instinctive desire for prospect and refuge as significant factors that influence the way that we read and respond to our surroundings. Appleton’s notion of prospect is one that encapsulates our desire to seek opportunity and interest from our surroundings. His notion of refuge refers to a desire to seek safety and shelter from within the same environment. Where Appleton seeks meaning from historical notions of landscape, subsequent writers and practitioners have applied prospect-refuge theory to broader categories that encapsulate a notion of environment, this is particularly the case within the field of architecture. Since prospect-refuge theory is largely forgotten within the field of fine art, this project seeks to test its currency as an applicable and useful tool for a contemporary visual art practitioner. My aim here is to test the limits of prospect-refuge theory by applying its universal principles to a set of parochial and idiosyncratic concerns that form part of my visual arts practice. Specifically I look at a personal set of associations that I have with the spaces of my suburban home and nature strip. I seek to use prospect-refuge theory as a means through which to contextualise these personal responses within a broader and more complex discourse.
- Description: Doctor of Philosophy
- Authors: Ryan, Vin
- Date: 2016
- Type: Text , Thesis , PhD
- Full Text:
- Description: Jay Appleton’s prospect-refuge theory asserts that there are innate, evolutionary factors that influence how we respond to the landscapes around us. Appleton identifies an instinctive desire for prospect and refuge as significant factors that influence the way that we read and respond to our surroundings. Appleton’s notion of prospect is one that encapsulates our desire to seek opportunity and interest from our surroundings. His notion of refuge refers to a desire to seek safety and shelter from within the same environment. Where Appleton seeks meaning from historical notions of landscape, subsequent writers and practitioners have applied prospect-refuge theory to broader categories that encapsulate a notion of environment, this is particularly the case within the field of architecture. Since prospect-refuge theory is largely forgotten within the field of fine art, this project seeks to test its currency as an applicable and useful tool for a contemporary visual art practitioner. My aim here is to test the limits of prospect-refuge theory by applying its universal principles to a set of parochial and idiosyncratic concerns that form part of my visual arts practice. Specifically I look at a personal set of associations that I have with the spaces of my suburban home and nature strip. I seek to use prospect-refuge theory as a means through which to contextualise these personal responses within a broader and more complex discourse.
- Description: Doctor of Philosophy
Assessing productive soil - landscapes in Victoria using digital soil mapping
- Authors: Robinson, Nathan
- Date: 2016
- Type: Text , Thesis , PhD
- Full Text:
- Description: Spatial soil information is used to support questions on agriculture and the environment from global to local scales. Historically, soil mapping has been used to inform and guide a multitude of land users with their decisions. Demand for specific spatial soil information is increasing in response from a wider range of users operating across agricultural and environmental domains. To satisfy these demands, users must be provided with practical and relevant spatial soil information. Novel approaches are required to deal with global deficiencies in available soil information. A major limitation to this is the plethora of incongruent legacy data with poor spatial and temporal coverage. This research study initially identifies the specific needs of users for spatial soil information with a focus on the requirements of biophysical modellers. Secondly, error sources that hamper Digital Soil Mapping (DSM) are identified, described and assessed using pH in practical and relevant examples. A final aim is to spatially predict soil properties (e.g. clay mineralogy) that underpin soil chemical behaviour. This is achieved by harmonising legacy data in combination with new spectroscopy techniques and a spatial inference approach. The spatial soil information needs of biophysical modellers in Victoria, Australia were found to be consistent with global needs for information including soil water characteristics, organic carbon and effective rooting depth. To accommodate stochastic and epistemic uncertainties in spatial soil information, uncertainty frameworks proved effective to deal with, and understand the limitations of legacy data in spatial inference models. Robust and reliable spectroscopic models for properties that are linked to functions and services delivered by soil were achieved and used in 3D spatial models. These findings will enable a tactical response through the delivery of pertinent spatial soil information that is contemporary, quality assured and sought by users. Learnings presented should enable producers of spatial soil information to be more comprehensive in their delivery of products that are easy to use, accessible and understood by a growing user community.
- Description: Doctor of Philosphy
- Authors: Robinson, Nathan
- Date: 2016
- Type: Text , Thesis , PhD
- Full Text:
- Description: Spatial soil information is used to support questions on agriculture and the environment from global to local scales. Historically, soil mapping has been used to inform and guide a multitude of land users with their decisions. Demand for specific spatial soil information is increasing in response from a wider range of users operating across agricultural and environmental domains. To satisfy these demands, users must be provided with practical and relevant spatial soil information. Novel approaches are required to deal with global deficiencies in available soil information. A major limitation to this is the plethora of incongruent legacy data with poor spatial and temporal coverage. This research study initially identifies the specific needs of users for spatial soil information with a focus on the requirements of biophysical modellers. Secondly, error sources that hamper Digital Soil Mapping (DSM) are identified, described and assessed using pH in practical and relevant examples. A final aim is to spatially predict soil properties (e.g. clay mineralogy) that underpin soil chemical behaviour. This is achieved by harmonising legacy data in combination with new spectroscopy techniques and a spatial inference approach. The spatial soil information needs of biophysical modellers in Victoria, Australia were found to be consistent with global needs for information including soil water characteristics, organic carbon and effective rooting depth. To accommodate stochastic and epistemic uncertainties in spatial soil information, uncertainty frameworks proved effective to deal with, and understand the limitations of legacy data in spatial inference models. Robust and reliable spectroscopic models for properties that are linked to functions and services delivered by soil were achieved and used in 3D spatial models. These findings will enable a tactical response through the delivery of pertinent spatial soil information that is contemporary, quality assured and sought by users. Learnings presented should enable producers of spatial soil information to be more comprehensive in their delivery of products that are easy to use, accessible and understood by a growing user community.
- Description: Doctor of Philosphy
Bi what means : Paratextual and filmic representations of bisexuality in contemporary cinema
- Authors: Benson, Chloe
- Date: 2016
- Type: Text , Thesis , PhD
- Full Text:
- Description: The question of how bisexuality, which is predominantly nonvisual and lacks a coherent cinematic code, can be represented in film remains a consistent concern within bisexual cinema studies. Scholars have tended to approach this problem by concentrating on the ways that the film text itself codes bisexuality or encourages a bisexual reading. This approach can offer important insights into the potential for and problems of screening bisexuality. However, this thesis argues that in order to more fully explore how bisexual meaning is constructed, critical attention must extend beyond the confines of the film text to engage in what Jonathon Gray describes as a form of “off-screen studies” (7). By developing a sustained engagement between paratextual theory and bisexual cinema studies this project develops a new methodological approach to filmic representations of bisexuality. Two samples of bisexual films and the official entryway paratexts - such as posters, trailers, and festival program notes - used to promote them are examined. These samples comprise films screened in the period from 2012-2014 on either the Melbourne general release circuit or at the Melbourne Queer Film Festival that have been identified online by viewers as incorporating bisexual meanings. Considering these texts in relation to their exhibition contexts, the thesis demonstrates that bisexual films can be found in diverse settings. It also establishes the impact that these settings have on the ways the films are framed paratextually. Close textual readings illustrate that paratexts can function as discrete texts that circulate bisexual meanings, as well as framings with the potential to prime viewers’ receptivity to onscreen bisexuality. The thesis reveals that the promotional impetus of paratexts can lead to the amplifying or subduing of bisexual readings across exhibition contexts and argues that an understanding of filmic bisexuality must acknowledge this. In sum, the thesis proposes that paratexts play a formative role in the production and circulation of bisexual meanings both on screen and off, within the niche realm of the queer film festival and on the general release circuit.
- Description: Doctor of Philosophy
- Authors: Benson, Chloe
- Date: 2016
- Type: Text , Thesis , PhD
- Full Text:
- Description: The question of how bisexuality, which is predominantly nonvisual and lacks a coherent cinematic code, can be represented in film remains a consistent concern within bisexual cinema studies. Scholars have tended to approach this problem by concentrating on the ways that the film text itself codes bisexuality or encourages a bisexual reading. This approach can offer important insights into the potential for and problems of screening bisexuality. However, this thesis argues that in order to more fully explore how bisexual meaning is constructed, critical attention must extend beyond the confines of the film text to engage in what Jonathon Gray describes as a form of “off-screen studies” (7). By developing a sustained engagement between paratextual theory and bisexual cinema studies this project develops a new methodological approach to filmic representations of bisexuality. Two samples of bisexual films and the official entryway paratexts - such as posters, trailers, and festival program notes - used to promote them are examined. These samples comprise films screened in the period from 2012-2014 on either the Melbourne general release circuit or at the Melbourne Queer Film Festival that have been identified online by viewers as incorporating bisexual meanings. Considering these texts in relation to their exhibition contexts, the thesis demonstrates that bisexual films can be found in diverse settings. It also establishes the impact that these settings have on the ways the films are framed paratextually. Close textual readings illustrate that paratexts can function as discrete texts that circulate bisexual meanings, as well as framings with the potential to prime viewers’ receptivity to onscreen bisexuality. The thesis reveals that the promotional impetus of paratexts can lead to the amplifying or subduing of bisexual readings across exhibition contexts and argues that an understanding of filmic bisexuality must acknowledge this. In sum, the thesis proposes that paratexts play a formative role in the production and circulation of bisexual meanings both on screen and off, within the niche realm of the queer film festival and on the general release circuit.
- Description: Doctor of Philosophy
Capital budgeting practices and firm performance : A comparative study of Australia and Sri Lanka
- Puwanenthiren, Pratheepkanth
- Authors: Puwanenthiren, Pratheepkanth
- Date: 2016
- Type: Text , Thesis , PhD
- Full Text:
- Description: This thesis disentangles two elements from the complex interdependent suite of key drivers of firm sophistication in capital-budgeting. Specifically, the relative sophistication of a firm (i.e. its nature) and the development level of the nation in which a firm is embedded (i.e. the nurture experienced by the firm). This research should enhance the development focus and process of nations (e.g., to what degree should national development be about raising the ability of individual firms or will raising national development act as a rising tide [that] raises all boats). The comparative data used in this study comes from 150 Australian (ASX200-index-listed) firms and 150 Sri Lankan (Colombo-stock-exchange-listed firms). The research questions are answered via a quantitative research design that uses primary and secondary data. The response rate to the questionnaire survey of firms was, 45 and 73 completed questionnaires from, respectively, Australia and Sri Lanka (an effective response rate of, respectively, 31.5 and 48.7 percent). Secondary data for 2003-12 are obtained from the ASX, CSE’s and SIRCA databases and are used to calculate return on assets, return on equity, Tobin Q, and earnings per share for the sampled firms. It was found that Australian firms tend to rely heavily on sophisticated capital-budgeting practices, but Sri Lankan relatively small firms prefer simple analysis methods and the larger firms tend to be as adept at sophisticated capital budgeting as their Australian counterparts. The choice of whether to use more sophisticated practices or simpler alternatives varies with a firm’s attributes as well as the level of economic and financial market development in its environment. Also, Australian firms tend to use capital-budget models with good-to-strong predictive power (except for ROE) and Sri Lankan firms tend to use capital-budget models with fair-to-poor predictive power. Further, the analysis of Australian firms tends to yield stronger and more statistically-significant results, than those generated by Sri Lankan firms.
- Description: Doctor of Philosophy
- Authors: Puwanenthiren, Pratheepkanth
- Date: 2016
- Type: Text , Thesis , PhD
- Full Text:
- Description: This thesis disentangles two elements from the complex interdependent suite of key drivers of firm sophistication in capital-budgeting. Specifically, the relative sophistication of a firm (i.e. its nature) and the development level of the nation in which a firm is embedded (i.e. the nurture experienced by the firm). This research should enhance the development focus and process of nations (e.g., to what degree should national development be about raising the ability of individual firms or will raising national development act as a rising tide [that] raises all boats). The comparative data used in this study comes from 150 Australian (ASX200-index-listed) firms and 150 Sri Lankan (Colombo-stock-exchange-listed firms). The research questions are answered via a quantitative research design that uses primary and secondary data. The response rate to the questionnaire survey of firms was, 45 and 73 completed questionnaires from, respectively, Australia and Sri Lanka (an effective response rate of, respectively, 31.5 and 48.7 percent). Secondary data for 2003-12 are obtained from the ASX, CSE’s and SIRCA databases and are used to calculate return on assets, return on equity, Tobin Q, and earnings per share for the sampled firms. It was found that Australian firms tend to rely heavily on sophisticated capital-budgeting practices, but Sri Lankan relatively small firms prefer simple analysis methods and the larger firms tend to be as adept at sophisticated capital budgeting as their Australian counterparts. The choice of whether to use more sophisticated practices or simpler alternatives varies with a firm’s attributes as well as the level of economic and financial market development in its environment. Also, Australian firms tend to use capital-budget models with good-to-strong predictive power (except for ROE) and Sri Lankan firms tend to use capital-budget models with fair-to-poor predictive power. Further, the analysis of Australian firms tends to yield stronger and more statistically-significant results, than those generated by Sri Lankan firms.
- Description: Doctor of Philosophy
Co-encapsulation of Omega-3 fatty acids and probiotic bacteria through complex coacervation
- Authors: Eratte, Divya
- Date: 2016
- Type: Text , Thesis , PhD
- Full Text:
- Description: The research described in this thesis investigated the microencapsulation of omega-3 oil and probiotic bacteria together in a protein-polysaccharide complex coacervate matrix. The synergistic or competitive interactions between the probiotic bacteria and omega-3 fatty acids when packaged in a single microcapsule was determined including how best to utilise such interaction to achieve improved oxidative stability of omega-3 fatty acid and better survival of the probiotic bacteria. Encapsulation and co-encapsulation of tuna oil (O) and Lactobacillus casei 431 (P) as models of omega-3 and probiotic bacteria, respectively, were carried out and the works is described in this thesis in five distinct sections. (1) The optimisation of the complex coacervation process between whey protein isolate (WPI) and gum Arabic (GA). (2) Microencapsulation of tuna oil (O) in WPI-GA complex coacervates followed by spray and freeze drying to produce microcapsules (WPI-O-GA). (3) Microencapsulation of probiotic bacteria L. casei 431 (P) in WPI-GA complex coacervates followed by spray and freeze drying to produce microcapsules (WPI-P-GA). (4) Co-encapsulation of omega-3 oil and L. casei 431 together in WPI-GA coacervate matrix followed by spray and freeze drying to produce co-microcapsules (WPI-P-O-GA). (5) In-vitro digestion evaluation of co-microcapsules and microcapsules to indicate bioavailability. The viability of L. casei was significantly higher in WPI-P-O-GA co-microcapsules than in WPI-P-GA microcapsules in both spray and freeze dried microcapsules. The oxidative stability of tuna oil was significantly higher in spray dried co-capsules. Also, co-microencapsulation increased the survivability of L. casei during simulated digestion. There was no significant influence observed on the release properties of omega-3 oil due to co-microencapsulation. However, the total omega-3 fatty acids in the released oil during in-vitro digestion were found to be higher, when co-microencapsulated. Hence, co-microencapsulation was shown to protect the L. casei and deliver both viable cells and omega-3 oil to human intestine without any significant adverse effect on their functionality and properties.
- Description: Doctor of Philosophy
- Authors: Eratte, Divya
- Date: 2016
- Type: Text , Thesis , PhD
- Full Text:
- Description: The research described in this thesis investigated the microencapsulation of omega-3 oil and probiotic bacteria together in a protein-polysaccharide complex coacervate matrix. The synergistic or competitive interactions between the probiotic bacteria and omega-3 fatty acids when packaged in a single microcapsule was determined including how best to utilise such interaction to achieve improved oxidative stability of omega-3 fatty acid and better survival of the probiotic bacteria. Encapsulation and co-encapsulation of tuna oil (O) and Lactobacillus casei 431 (P) as models of omega-3 and probiotic bacteria, respectively, were carried out and the works is described in this thesis in five distinct sections. (1) The optimisation of the complex coacervation process between whey protein isolate (WPI) and gum Arabic (GA). (2) Microencapsulation of tuna oil (O) in WPI-GA complex coacervates followed by spray and freeze drying to produce microcapsules (WPI-O-GA). (3) Microencapsulation of probiotic bacteria L. casei 431 (P) in WPI-GA complex coacervates followed by spray and freeze drying to produce microcapsules (WPI-P-GA). (4) Co-encapsulation of omega-3 oil and L. casei 431 together in WPI-GA coacervate matrix followed by spray and freeze drying to produce co-microcapsules (WPI-P-O-GA). (5) In-vitro digestion evaluation of co-microcapsules and microcapsules to indicate bioavailability. The viability of L. casei was significantly higher in WPI-P-O-GA co-microcapsules than in WPI-P-GA microcapsules in both spray and freeze dried microcapsules. The oxidative stability of tuna oil was significantly higher in spray dried co-capsules. Also, co-microencapsulation increased the survivability of L. casei during simulated digestion. There was no significant influence observed on the release properties of omega-3 oil due to co-microencapsulation. However, the total omega-3 fatty acids in the released oil during in-vitro digestion were found to be higher, when co-microencapsulated. Hence, co-microencapsulation was shown to protect the L. casei and deliver both viable cells and omega-3 oil to human intestine without any significant adverse effect on their functionality and properties.
- Description: Doctor of Philosophy