Insight into key obstacles and technological strategy for enzymatic digestion of full cellulose fraction from poplar sawdust

Guo, Jianming; Li, Jing; Liu, Dylan; Xu, Yong

Title: Insight into key obstacles and technological strategy for enzymatic digestion of full cellulose fraction from poplar sawdust
Creator: Guo, Jianming; Li, Jing; Liu, Dylan; Xu, Yong
Date: 2024
Type: Text; Journal article
Identifier: http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/199893
Identifier: vital:19275
Identifier: https://doi.org/10.1016/j.biortech.2023.129994
Identifier: ISSN:0960-8524 (ISSN)
Abstract: Lignocellulosic biomass mainly consists of hemicellulose, lignin, and cellulose, which differently affect the enzymatic digestibility of cellulose. As for the typical representative for inert woody biomass, three components of cellulose were proposed conceptually for poplar sawdust, i.e., active cellulose, inert cellulose, and resistant cellulose. Dilute sulfuric acid pretreatment, hydrogen peroxide-sulfuric acid delignification, and sulfuric acid-assisted glycerol swelling were, respectively, proven to break the three obstacle mechanisms that affect the cellulase of poplar. The removal of key obstacles improved the cellulase digestibility of poplar enzyme-hydrolyzed residues by 188.7 %, and glucose yield increased from 34.6 % to 99.9 %. Therefore, a total of 39.5 g glucose was obtained from 100 g poplar sawdust by integrating the above three technologies. This work presented insight into and removed the key obstacles to enzymatic digestibility of poplar cellulose and developed an integrated technology to effectively convert full cellulose fraction to glucose from woody biomass. © 2023 Elsevier Ltd
Publisher: Elsevier Ltd
Relation: Bioresource Technology Vol. 391, no. (2024), p.
Rights: All metadata describing materials held in, or linked to, the repository is freely available under a CC0 licence
Subject: 3001 Agricultural biotechnology; 3106 Industrial biotechnology; 3107 Microbiology; Hydrogen peroxide-sulfuric acid delignification; Poplar cellulose; Sulfuric acid pretreatment; Sulfuric acid-assisted glycerol swelling
Reviewed
Funder: The research was supported by the National Key R&D Program of China (2022YFD1300903), and the National Natural Science Foundation of China (32171730, 32211530071).

Hits: 87
Visitors: 87
Downloads: 0